Agilent Technologies 6500 Series Manual de funcionamiento

Agilent Technologies

Agilent 6500 Series

Q-TOF LC/MS system

Maintenance Guide

Research Use Only. Not for use in Diagnostic

Procedures.

Agilent 6500 Series Q-TOF LC/MS Maintenance Guide

Notices

No part of this manual may be reproduced in

any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G2581-90065

Edition

Revision B, December 2011

Printed in USA

Agilent Technologies, Inc.

5301 Stevens Creek Blvd.

Santa Clara, CA 95051

Warranty

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ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

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liable for errors or for incidental or

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Items) and DFARS 227.7202-3 (Rights in

Commercial Computer Software or Com-

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Safety Notices

CAUTION

A CAUTION notice denotes a haz-

ard. It calls attention to an operat-

ing procedure, practice, or the like

that, if not correctly performed or

adhered to, could result in damage

to the product or loss of important

data. Do not proceed beyond a

CAUTION notice until the indicated

conditions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in personal injury or death. Do not

proceed beyond a WARNING

notice until the indicated condi-

tions are fully understood and

met.

Patents

This product is covered by U.S. patents

6,107,628 and 6,803,565 licensed from

Battelle Memorial Institute.

Agilent 6500 Series Q-TOF LC/MS Maintenance Guide 3

Content

1 Basic Operation 7

Starting and Shutting Down 13

To start the system in Standby mode 15

To change from Standby to On 18

To change from On to Standby 18

To shut down the instrument 19

Changing Ion Sources 21

To remove an electrospray source 21

To remove the ESI with Agilent Jet Stream Technology 22

To remove a multimode source 22

To remove an APCI or APPI source 23

To remove a nano ESI source 24

To remove a dual nano ESI source 25

To install an electrospray source 26

To install an APCI source 27

To install an APPI source 28

To install a nano ESI, dual nano ESI or multimode source or HPLC-Chip/MS

interface 28

To install the Agilent Jet Stream inlet assembly 29

2 Maintenance 31

Calibrant Delivery System and Divert Valve 32

To prepare tuning mix 35

To prepare the reference mass solution 40

To check calibrant levels 43

To fill a tuning mix or reference mix bottle 44

To check for leaks 45

To replace the LC filter elements 46

To replace the MS selection valve rotor seal 47

4 Agilent 6500 Series Q-TOF LC/MS Maintenance Guide

Contents

Electrospray Ion Source 49

To flush the nebulizer 49

To clean the electrospray spray chamber daily 50

To clean the electrospray spray chamber weekly 53

To remove the electrospray nebulizer 57

To replace the electrospray nebulizer needle in the analytical or the reference

nebulizer sprayer 58

To adjust the electrospray nebulizer needle 61

To reinstall the electrospray nebulizer 63

To remove the desolvation assembly 64

To clean skimmer 1 (for 6520/6530/6538/6540) 66

To reinstall the desolvation assembly 68

ESI with Agilent Jet Stream Technology 69

To flush the nebulizer daily 69

To flush the nebulizer monthly 70

To clean the spray chamber daily for the ESI with Agilent Jet Stream 71

To clean the spray chamber weekly for the ESI with Agilent Jet Stream 74

To remove the nebulizer for the ESI with Agilent Jet Stream 78

To replace the nebulizer needle for the ESI with Agilent Jet Stream 79

To adjust the nebulizer needle for the ESI with Agilent Jet Stream 83

To reinstall the nebulizer for the ESI with Agilent Jet Stream 85

APCI Source 86

To clean the APCI spray chamber daily 86

To clean the APCI spray chamber weekly 89

To remove the APCI nebulizer 91

To replace the APCI nebulizer needle 92

To adjust the APCI nebulizer needle 95

To reinstall the APCI nebulizer 97

To clean the corona needle 98

To replace the corona needle 100

Multimode Source and HPLC-Chip/MS Interface 102

To clean the multimode source daily 102

Contents

Agilent 6500 Series Q-TOF LC/MS Maintenance Guide 5

To clean the multimode source weekly 104

To adjust the multimode nebulizer 106

To change HPLC-Chip capillaries 109

Ion Transfer Capillary 113

To remove the capillary 113

To clean the capillary 115

To reinstall the capillary 118

To clean skimmer 1 (for 6520/6530/6538/6540) 119

Manifold 121

To open the manifold 121

To close the manifold 122

To clean the ion optics assembly 123

High Pressure Ion Funnel (6550 only) 132

To remove the high pressure ion funnel 132

To clean the high pressure ion funnel 135

To reinstall the high pressure ion funnel process 138

Vacuum System 139

To check the rough pump fluid level 139

To check the oil mist filter 141

To add rough pump fluid 142

To replace the rough pump fluid 144

To replace the fuses 146

3 Reference 149

Safety 150

Environmental Conditions 151

Replaceable Fuses 151

6 Agilent 6500 Series Q-TOF LC/MS Maintenance Guide

Contents

Agilent 6500 Series Q-TOF LC/MS

Maintenance Guide

Agilent Technologies

Basic Operation

Starting and Shutting Down 13

Changing Ion Sources 21

This chapter describes procedures for the basic operation of your Q-TOF

LC/MS instrument.

Figure 1 6520 Q-TOF LC/MS covers. The main power switch is behind the service panel.

Source

CDS cover

Service panel

Power switch

Flight tube

Front cover

8 Agilent 6500 Series Q-TOF LC/MS Maintenance Guide

1 Basic Operation

Figure 2 6530 with Agilent Jet Stream Technology

For more details, see the site preparation guide that is sent to customers

before installation. If you move the instrument, you must follow and repeat the

steps detailed in the site preparation guide.

CDS cover

Service panel

Power switch

Flight tube

Front cover

Agilent 6500 Series Q-TOF LC/MS Maintenance Guide 9

Basic Operation 1

Figure 3 6538/6540 Q-TOF LC/MS

CDS cover

Service panel

Power switch

Flight tube

Front cover

10 Agilent 6500 Series Q-TOF LC/MS Maintenance Guide

1 Basic Operation

Figure 4 6550 Q-TOF LC/MS

Service panel

Power switch

Flight tube

Front cover

CDS bottles for tuning

and reference mixes

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