Agilent Technologies 1220 Infinity II LC Manual de usuario

Agilent Technologies

Agilent InfinityLab LC Series

1220 Infinity II LC System

User Manual

1220 Infinity II LC System User Manual

Notices

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from Agi-

lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G4280-90020 Rev. B

Edition

09/2016

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

Warranty

The material contained in this docu-

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in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

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liable for errors or for incidental or

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receive no greater than Restricted Rights as

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1987). U.S. Government users will receive

no greater than Limited Rights as defined in

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applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

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met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

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WARNING notice until the indi-

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understood and met.

1220 Infinity II LC System User Manual 3

In This Guide...

This manual covers the Agilent 1220 Infinity II LC System configurations:

•G4286B

•G4288B/C

•G4290B/C

•G4294B

1Introduction

This chapter provides an overview of the Agilent 1220 Infinity II LC available

configurations.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical

and performance specifications.

3 Installation

This chapter provides an overview on shipment content and installation.

4 LAN Configuration

This chapter provides information on connecting the instrument to the Agilent

ChemStation PC.

5 Solvent Delivery System Description

This chapter provides an overview on the operational principles of the solvent

delivery system (pump and optional degasser).

6InjectionSystemDescription

This chapter provides an overview of the operational principles of the

injection systems: manual injector and autosampler.

4 1220 Infinity II LC System User Manual

In This Guide...

7 Column Oven Description

This chapter provides an overview of the operational principles of the column

oven.

8 Detector Description

This chapter provides an overview of the operational principles of the

detector.

9 Test Functions and Calibration

This chapter describes the tests, calibrations and tools that are available with

the Instrument Utilities software or the Lab Advisor (Service and Diagnostics

Section).

10 Error Information

This chapter provides information on the error messages that might be

displayed, and gives the possible causes and suggestions on their solutions.

11 Maintenance and Repair

This chapter provides general information on maintenance and repair of the

instrument.

12 Parts for Maintenance and Repair

This chapter provides information on parts for maintenance and repair.

13 Upgrading the 1220 Infinity II LC

This chapter provides information for upgrading the LC system.

14 Identifying Cables

This chapter provides information on cables used with the Agilent 1200

Infinity Series modules.

15 Appendix

This chapter provides addition information on safety, legal and web.

1220 Infinity II LC System User Manual 5

Contents

1Introduction 9

Agilent 1220 Infinity II LC Configurations 10

Agilent 1220 Infinity II LC VL Configurations 11

Early Maintenance Feedback 12

2 Site Requirements and Specifications 15

Site Requirements 16

Physical Specifications 19

Performance Specifications 20

3 Installation 29

Unpacking Your System 30

Installing the Hardware 35

Connecting and Configuring the Instrument to the Chromatographic Data

System 44

Connecting the Agilent 1220 Infinity II LC to the PC 45

The Instrument Utility / Lab Advisor Software 47

Configuration of the Instrument After an Upgrade Installation 48

Priming the System and Performing the ‘Installation Check’ 49

Performing a ‘Checkout Run’ 50

Remove the Restriction Capillary 51

Install a Column 53

Remove a Column 55

Install the Restriction Capillary 57

4 LAN Configuration 59

To do firs t 60

TCP/IP parameter configuration 62

Configuration Switches 63

Initialization mode selection 64

Dynamic Host Configuration Protocol (DHCP) 66

Link configuration selection 69

6 1220 Infinity II LC System User Manual

Contents

Automatic Configuration with BootP 70

Storing the settings permanently with Bootp 79

Manual Configuration 80

5 Solvent Delivery System Description 85

Overview 86

Degasser 87

Principles of Operation 88

Compressibility Compensation 92

Variable Stroke Volume 95

Using the Pump 96

6 Injection System Description 99

Manual Injector 100

Autosampler 103

7 Column Oven Description 115

Column Oven 116

8 Detector Description 117

Detector Types 118

Agilent 1220 Infinity II LC Variable Wavelength Detector (VWD) 119

Agilent 1220 Infinity II LC Diode Array Detector (DAD) 120

Match the Flow Cell to the Column 136

9 Test Functions and Calibration 141

1220 Infinity II LC System 143

Solvent Delivery System 145

Autosampler 154

Column Oven 160

Variable Wavelength Detector (VWD) 162

Diode Array Detector (DAD) 171

1220 Infinity II LC System User Manual 7

Contents

10 Error Information 193

What are Error Messages? 196

General Error Messages 197

Pump Error Messages 203

Autosampler Error Messages 216

General Detector Error Messages 226

VWD Detector Error Messages 229

DAD Detector Error Messages 235

11 Maintenance and Repair 241

PM Scope of Work and Checklist 243

Cautions and Warnings 244

Solvent Delivery System 246

Manual Injector 267

Autosampler 271

Variable Wavelength Detector (VWD) 290

Diode Array Detector (DAD) 302

Algae Growth in HPLC Systems 323

Replacing the Module’s Firmware 325

12 Parts for Maintenance and Repair 327

1220 Infinity II LC System 328

Solvent Delivery System 331

Injection System 340

Column Oven 347

Detector 348

13 Upgrading the 1220 Infinity II LC 355

Oven Upgrade 356

Install the (optional) External Tray 359

Install the Active Inlet Valve 363

8 1220 Infinity II LC System User Manual

Contents

14 Identifying Cables 367

Cable Overview 368

Analog Cables 370

Remote Cables 372

BCD Cables 375

CAN/LAN Cables 377

Agilent 1200 module to PC 378

15 Appendix 379

General Safety Information 380

Solvent Information 383

Radio Interference 385

UV Radiation 386

Sound Emission 387

Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) 388

Declaration of Conformity for HOX2 Filter 389

Agilent Technologies on Internet 390

1220 Infinity II LC System User Manual

Agilent Technologies

Introduction

Agilent 1220 Infinity II LC Configurations 10

Agilent 1220 Infinity II LC VL Configurations 11

Early Maintenance Feedback 12

EMF counters for the pump 12

EMF counters for the autosampler 12

EMF counters for the variable wavelength detector 13

EMF counters for the diode array detector 14

This chapter provides an overview of the Agilent 1220 Infinity II LC available

configurations.

10 1220 Infinity II LC System User Manual

Introduction

Agilent 1220 Infinity II LC Configurations

Available configurations of Agilent 1220 Infinity II LC

The Agilent 1220 Infinity II LC is available in four different configurations.

Possible components include isocratic pump, dual-channel gradient pump

(with degasser), manual injector, autosampler, column oven and detector.

Each configuration comes with at least one pump, one injection system and

one detector and includes Agilent Instrument Utilities Software.

A Solvent Selection Valve (SSV) Upgrade Kit (G4280-68708) is available.

A 1220 Infinity II AIV Upgrade Kit (G5699B) is available to handle

applications with high buffer concentration.

Variable

wavelength

detector

Variable

wavelength

detector

Variable

wavelength

detector

Isocratic pump Gradient pump Gradient pump Gradient pump

Manual injector Manual injector Autosampler Autosampler

Column oven

Diode

array

detector

Column oven

G4286B G4288B G4290B G4294B

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