Agilent Technologies Varian CP-8400 Manual de usuario

Varian, Inc.

2700 Mitchell Drive

Walnut Creek, CA 94598-1675/USA

Varian, Inc. 2003 Printed in the Netherlands 03-914852-00:Rev. 4.1

CP-8400 AutoSampler and

CP-8410 AutoInjector

Operator's Manual

NOTICE: Varian, Inc. was acquired by Agilent

Technologies in May 2010. This document is provided

as a courtesy but is no longer kept current and thus

will contain historical references to Varian. For more

information, go to www.agilent.com/chem.

CP-8400 AutoSampler & CP-8410 AutoInjector i

Table of Contents

Introduction.............................................................................................................................. 1

Installing the CP-8400/8410 .................................................................................................... 3

Configuring your CP-8400/8410 to use SPME ...................................................................... 9

Setup and Calibration of the CP-8400/8410 ........................................................................ 11

Vial 0 / Vial 1 Calibration ......................................................................................................... 17

Vial Calibration tool.................................................................................................................. 19

Injector Calibration................................................................................................................... 21

Operation................................................................................................................................ 23

Parameters and Quick Reference ........................................................................................... 23

Automation Page Parameters ................................................................................................. 28

Single Method Automation Parameters ............................................................................ 28

Method Sequence Table Parameters ............................................................................... 32

Priority Sample .................................................................................................................. 34

Method Parameters ................................................................................................................. 35

Standard Split/Splitless and Standard On-Column Sampling Modes ............................... 36

Neat Sampling Mode......................................................................................................... 38

Volatile Sampling Mode .................................................................................................... 40

Viscous Sampling Mode.................................................................................................... 42

SPME Sampling Mode ...................................................................................................... 44

User Defined Sampling Mode ........................................................................................... 47

Instrument Menu...................................................................................................................... 54

CP-8400/8410 Status .............................................................................................................. 55

Special Uses........................................................................................................................... 57

Dual/Duplicate Mode Operation .............................................................................................. 57

Dual Run ........................................................................................................................... 57

Duplicate Run.................................................................................................................... 58

Assigning Methods to Vial Ranges.......................................................................................... 58

Automated Metering of Internal Standard................................................................................ 59

Priority Sample ........................................................................................................................ 59

CP-8400 AutoSampler & CP-8410 AutoInjector 1

Introduction

We strongly recommend that you read the entire manual before you start

using your CP-8400 AutoSampler or CP-8410 AutoInjector. The

CP-3800 will sense whether you have a CP-8400 or CP-8410 installed

automatically and will sense if the Auto Sampler is set to perform liquid

sampling with a syringe or SPME sampling. You will be presented with

the appropriate screens and limits for the specific Auto Sampler and

sampling mode configured on powering up the GC and Auto Sampler.

The CP-8400 is a 100 vial capacity AutoSampler designed to be used

with the CP-3800 GC. The CP-8410 is an AutoInjector based on the

CP-8400; it has a manually positioned tray and can accommodate up to

10, 6, and 5 each of 2 mL, 5 mL and 10 mL vials, respectively. Both the

AutoSampler and AutoInjector can inject into up to two injectors; the

injection may be made into either one or the other of the injectors or into

both (either from the same vial or from adjacent vials) during the same

run. You may select to sample just before injecting or you may choose to

have the Auto Sampler prepare the next injection before the current run

ends (Prep Ahead). Both the CP-8400 and CP-8410 are

preprogrammed with several injection modes (Standard On-Column,

Standard Split/Splitless, Viscous, Volatile, and Neat) that are optimized

for most common types of samples. The User Defined mode allows

access to all parameters and should be used with discretion since

injection accuracy and precision may be adversely affected by incorrect

choice of certain parameters. The SPME mode requires different

hardware (optional) and is selected through a switch on the Auto

Sampler’s syringe carriage. If the SPME mode is selected the other

injection modes will not be presented to you.

Once your CP-8400 or CP-8410 is installed and calibrated, you may

begin running immediately. To perform a single injection, complete the

following steps:

1. Press the green Start button located at the upper left corner of the

CP-3800 keypad.

2. Enter the vial number, number of replicate injections, the injection

volume, and which injector you wish to use.

3. Press the AutoInject softkey.

2 03-914852-00:4

Your AutoSampler or AutoInjector will execute the desired injection.

To set up an automated series of injections, simply complete the

following steps:

1. Press the Automation Select/Edit button on the CP-3800, choose

either Single Method automation to run all of your samples with a

single method, or choose Method Sequence Automation to run

specific methods with specific vials.

2. Press the Automation Select/Edit button again and press 2to

select Single Method, or 3to select Method Sequence and enter

the methods you wish to use, the vial range, the amount you

want to inject and the injector you wish to use (if you have more

than one injector installed).

3. Place your samples into the AutoSampler carrousel.

4. Press the Start button.

This manual will cover AutoSampler installation, software setup and

calibration to the correct sampling and injection positions, a description

of the operating parameters, and some examples of how you may wish

to use the CP-8400/8410 to accomplish your analyses. If, after reading

this manual you still have questions or encounter a problem that is not

covered, please contact Technical Support for further assistance.

CP-8400 AutoSampler & CP-8410 AutoInjector 3

Installing the CP-8400/8410

To use the CP-8400 with the CP-3800 Gas Chromatograph, your

CP-3800 must have Version 3.2 or later software installed. The CP-8410

requires version 3.3 or later. To use SPME mode sample introduction

with either the CP-8400 or CP-8410 you must have Version 3.3.5 or later

software installed. To check the software version installed in your GC,

press the Status key on the GC keyboard, press the Enter key to show

instrument status, and then press the Software Version softkey. The

software version will be shown on the CP-3800 display. If your GC was

manufactured before October 1999 and you have two or more injectors

installed, you will need to schedule a service call with your Varian

Technical Support Representative to have the injector positions

relocated so that the CP-8400 can effectively use them.

Power Requirements

19-24 Vac provided by Varian GC auxiliary (fused)

Input current max. 1A

Environmental Conditions

Temperature range 5 to 40 °C

Relative humidity 0 to 95%

Before installing the CP-8400 or CP-8410, make certain your CP-3800 is

equipped with the appropriate top cover. This cover will have a long

rectangular opening for the installation of the injector locator plate,

markings indicating the front and rear installation positions for the

AutoSampler, and a small rectangular opening to route the

CP-8400/8410 power/communication cable through.

There are two positions on the CP-3800 in which the CP-8400/8410 may

be installed: front and rear. The front position allows the CP-8400/8410

to use the front and middle injectors. The rear position allows the

CP-8400/8410 to use the middle and rear injectors. The CP-8400/8410 is

aligned to the top cover of the CP-3800 by a pair of pins protruding from

the bottom of the AutoSampler/AutoInjector base. These pins will fit into

holes on the top cover. The base is secured to the top cover via two

screws at the rear and three screws underneath the AutoSampler

carrousel/AutoInjector sample tray. The figures that accompany this

description depict a CP-8400 AutoSampler. They apply equally to both

the CP-8400 and CP-8410.

4 03-914852-00:4

CP-8400/8410 CP-3800 Top Cover

To install the CP-8400/8410, follow the steps below:

WARNING:

SHOCK HAZARD

Dangerous voltage exposed. Turn the CP-3800 GC power off. Failure to do

so may result in accidental contact with dangerous voltage.

1. Power down the GC and any other attached devices. Remove

any old equipment or old autosamplers from the top of the GC.

Place the CP-8400/8410 near the GC, manually rotate the

Injector tower so that it does not obstruct the carrousel.

2. Remove the old CP-3800 top cover by lifting up and removing

the Detector cover; set it aside in a location that is out of the

way. Remove the left side top cover by lifting the rear of the

cover and sliding it towards the rear of the GC and up. Remove

the 4 screws holding the injector cover down on the top of the

GC. Retain the screws and set the old top cover aside.

3. Locate the CP-8400/8410 power/communication cable and the

CP-8400/ CP-3800 top cover. Route the single D-shell connector

through the hole from the bottom of the cover to the top so that

the single D-shell is available from the top of the cover. Route

the power cable and CP-3800 25 pin D-shell connector across

the top of the GC to the left side of the GC. Plug the power cable

into Aux 1 (J46) and the 25-pin D-shell into the S.I.D. 1 (J4)

connector.

Injector Locato

Plate

CP-8400 in Front Position

Installing the CP-8400/8410

CP-8400 AutoSampler & CP-8410 AutoInjector 5

CP-8400/8410 Power/Communication Connection to CP-3800

Cable Routing for CP-3800

Back of CP-8400/8410

CP-8400/841-

Power Switch

ux 1

Cables routed through

hole in cove

6 03-914852-00:4

4. If the Injector locator plate is installed in the top cover, remove it

by removing the two screws located on either end of the locator

plate. Install the top cover so that the pins on the top cover fit

into the corresponding slots on the CP-3800 chassis. Screw the

top cover in place.

5. Install the Injector locator plate by fitting the circular holes over

the injectors. You may need to push the locator plate just a little

bit in order to get the injectors to fit. Do not use excessive force.

If the injectors do not fit conveniently, loosen the screws holding

the injector(s) to the CP-3800 chassis and adjust the injector(s)

positions so that they conveniently fit into the locator plate. The

top cover has small access holes to allow you to loosen the

retaining screws on the injectors. You may need to unscrew the

injector nut and remove the inject switch to have unimpeded

access to the injector retaining screws; replace the injector nut (if

removed) so that you have proper alignment when the locator

plate is installed. Once the injector(s) are properly aligned, screw

down the alignment plate and then screw down the injector

retaining screws. Remove the locator plate, replace the inject

switch on each of the injector(s) and reinstall the locator plate.

6. Place the CP-8400/8410 onto the top cover, aligning it with either

the front or rear mounting position as appropriate. Locate and

install the two small screws provided with your CP-8400/8410

and screw down the rear tab.

7. Locate the three long screws and install in appropriate screw

location under the carrousel (note that the CP-8410 does not

have the rollers or central hub but will have the same screw

locations).

Installing the CP-8400/8410

CP-8400 AutoSampler & CP-8410 AutoInjector 7

Carrousel Removed to Show Mounting Screw Locations

8. Plug the D-shell connector of the power/communications cable

into the CP-8400/8410.

9. Remove protective shipping materials from the injector carriage

and install the syringe into the AutoSampler injector tower by

opening the clear plastic door, carefully inserting the syringe into

its holder and the needle through its guide, then fasten the

plunger screw on the top and snap the retaining clip over the

syringe.

Screw Holes: Used

in mounting both

front and rear

utoSampler

positions.

Screw hole used in

mounting rear position

only.

Screw hole used in

mounting front position

only.

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