Agilent Technologies 1260 Infinity Manual de usuario

User Manual

Agilent 1260 Infinity

Variable Wavelength

Detector

2 Agilent 1260 Infinity Variable Wavelength Detector User Manual

Notices

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Manual Part Number

G1314-90011

Edition

Edition 06/2010

Printed in Germany

Agilent Technologies

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76337 Waldbronn, Germany

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operating procedure, practice, or

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Agilent 1260 Infinity Variable Wavelength Detector User Manual 3

In This Manual…

This manual covers the Agilent 1260 Infinity Variable

Wavelength Detectors

1 Introduction to the Variable Wavelength Detector

This chapter gives an introduction to the detector, instrument

overview and internal connectors.

2 Site Requirements and Specifications

This chapter gives information on environmental requirements,

physical and performance specifications.

3 Installing the Detector

This chapter provides information on unpacking, checking on

completeness, stack considerations and installation of the

module.

4 Using the Detector

This chapter provides information on how to set up the detector

for an analysis and explains the basic settings.

5 How to optimize the detector

This chapter gives hints on how to select the detector

parameters and the flow cell.

6 Troubleshooting and Diagnostics

This chapter describes the detector’s built in diagnostic signals.

G1314B Agilent 1260 Infinity Variable Wavelength Detector VL

G1314C Agilent 1260 Infinity Variable Wavelength Detector VL+

4 Agilent 1260 Infinity Variable Wavelength Detector User Manual

7 Error Information

This chapter describes the meaning of detector error messages,

and provides information on probable causes and suggested

actions how to recover from error conditions.

8TestFunctions

This chapter describes the detector’s built in test functions.

9 Maintenance and Repair

This chapter provides general information on maintenance and

repair of the detector.

10 Maintenance

This chapter describes the maintenance of the detector.

11 Parts and Materials for Maintenance

This chapter provides information on parts for maintenance.

12 Identifying Cables

This chapter provides information on cables used with the

Agilent 1260 Infinity modules.

A Appendix

This chapter provides addition information on safety, legal and

web.

Agilent 1260 Infinity Variable Wavelength Detector User Manual 5

Contents

1 Introduction to the Variable Wavelength Detector

Introduction to the Detector 12

Optical System Overview 13

Electrical Connections 17

Serial Number Information 19

Instrument Layout 20

Early Maintenance Feedback (EMF) 21

EMF Counter 21

Using the EMF Counters 21

Interfaces 23

Overview Interfaces 25

Setting the 8-bit Configuration Switch 29

Setting the 8-bit Configuration Switch (On-Board LAN) 29

Setting the 8-bit Configuration Switch (without On-Board

LAN) 31

Communication Settings for RS-232C 32

Special Settings 34

2 Site Requirements and Specifications

Site Requirements 38

Power Consideration 38

Power Cords 39

Bench Space 40

Environment 40

Physical Specifications 42

6 Agilent 1260 Infinity Variable Wavelength Detector User Manual

Performance Specifications 43

Specification Conditions 45

3 Installing the Detector

Unpacking the Detector 48

Delivery Checklist 49

Detector Accessory Kit Contents 49

Optimizing the Stack Configuration 50

One Stack Configuration 50

Two Stack Configuration 53

Installing the Detector 55

Flow Connections to the Detector 58

4 Using the Detector

Setting up an Analysis 62

Before Using the System 62

Requirements and Conditions 64

Optimization of the System 66

Preparing the HPLC System 67

Running the Sample and Verifying the Results 76

Special Settings of the Detector 77

Control Settings 77

Online Spectra 78

Scanning with the VWD 79

Analog Output Settings 81

Special Setpoints 82

Peakwidth Settings 83

Optimizing the Detector 85

Agilent 1260 Infinity Variable Wavelength Detector User Manual 7

5 How to optimize the detector

Optimizing the Detector Performance 88

Match the Flow Cell to the Column 88

Set the Detector Parameters 91

6 Troubleshooting and Diagnostics

Overview of the Detector’s Indicators and Test Functions 94

Status Indicators 95

Power Supply Indicator 95

Module Status Indicator 96

Available Tests vs User Interfaces 97

Agilent LabAdvisor Software 98

7 Error Information

What Are Error Messages 100

General Error Messages 101

Timeout 101

Shut-Down 102

Remote Timeout 102

Synchronization Lost 103

Leak 103

Leak Sensor Open 104

Leak Sensor Short 104

Compensation Sensor Open 105

Compensation Sensor Short 105

Fan Failed 106

Open Cover 106

Detector Error Messages 107

Lamp Current Missing 107

Lamp Voltage Missing 107

8 Agilent 1260 Infinity Variable Wavelength Detector User Manual

Lamp Ignition Failed 108

Heater Current Missing 108

Calibration Failed 109

Holmium Oxide Test Failed 110

Grating/Filter Motor Test Failed 110

Wavelength Check Failed 111

Filter Check Failed 111

8TestFunctions

Available Tests versus Interfaces 114

Intensity Test 115

Checking the Photocurrent 117

Wavelength Verification/Calibration 118

ASTM Drift and Noise Test 122

Quick Noise Test 123

Dark Current Test 124

Holmium Oxide Test 126

D/A Converter (DAC)Test 128

Grating and Filter Motor Test 131

Test Chromatog r a m 133

Procedure Using the Agilent LabAdvisor 133

9 Maintenance and Repair

Introduction to Maintenance 136

Warnings and Cautions 137

Agilent 1260 Infinity Variable Wavelength Detector User Manual 9

10 Maintenance

Overview of Maintenance 140

Cleaning the Module 141

Exchanging a Lamp 142

Exchanging a Flow Cell 145

Repairing the Flow Cells 148

Using the Cuvette Holder 151

Correcting Leaks 154

Replacing Leak Handling System Parts 155

Replacing the Interface Board 156

Replacing the Detector’s Firmware 157

11 Parts and Materials for Maintenance

Overview of Maintenance Parts 160

Standard Flow Cell 161

Micro Flow Cell 163

Semi-micro Flow Cell 164

High Pressure Flow Cell 166

Cuvette Holder 167

Leak Parts 168

Accessory Kit 169

10 Agilent 1260 Infinity Variable Wavelength Detector User Manual

12 Identifying Cables

Cable Overview 172

Analog Cables 174

Remote Cables 176

BCD Cables 179

CAN / LAN Cables 181

RS-232 Cable 182

External Contact Cable 183

A Appendix

General Safety Information 186

Safety Standards 186

Safety Symbols 188

Lithium Batteries Information 189

Radio Interference 190

Sound Emission 191

UV-Radiation 192

Solvent Information 193

Declaration of Conformity for HOX2 Filter 195

Agilent Technologies on Internet 196

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