Agilent Technologies 1260 Infinity II Quaternary System Manual de usuario

Agilent Technologies

Agilent InfinityLab LC Series

1260 Infinity II Quaternary

System

Manual and Quick Guide

1260 Infinity II Quaternary System - Manual and Quick Guide

Notices

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tion into a foreign language) without prior

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lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G7111-90300

Edition

04/2016

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

Warranty

The material contained in this docu-

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in future editions. Further, to the max-

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Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

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met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

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result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

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1260 Infinity II Quaternary System - Manual and Quick Guide 3

In This Book

This book describes the Agilent InfinityLab LC Series 1260 Infinity II

Quaternary System.

1Introduction

This chapter gives an introduction to the Agilent 1260 Infinity II LC, the

underlying concepts and the features of the Agilent 1260 Infinity II LC.

2 Configuration Settings

This chapter describes how to configure the system.

3QuickStartGuide

This chapter provides information on running an Agilent 1260 Infinity II LC

System.

4 Parts and Consumables

This chapter provides information on additional parts and consumables.

5 Appendix

This chapter provides addition information on safety, legal, and web.

4 1260 Infinity II Quaternary System - Manual and Quick Guide

Contents

1Introduction 5

Product Description 6

Features of the Agilent 1260 Infinity II Quaternary LC 7

System Components 8

Solutions 20

Optimizing the Stack Configuration 24

Leak and Waste Handling 30

2 Configuration Settings 37

General Information on LAN Configuration 38

Instrument Configuration 39

Lab Advisor 41

3QuickStartGuide 47

Best Practices 48

Prepare a Run 49

Check Out the System 56

4 Parts and Consumables 69

Tool Kit 70

A-Line Quick Connect and Quick Turn Fittings 72

5 Appendix 77

Safety 78

Agilent Technologies on Internet 84

1260 Infinity II Quaternary System - Manual and Quick Guide

Agilent Technologies

Introduction

Product Description 6

Features of the Agilent 1260 Infinity II Quaternary LC 7

System Components 8

Quaternary Pump G7111B 9

Quaternary Pump VL G7111A 10

Isocratic Pump G7110B 11

Multisampler G7167A 12

Vialsampler G7129A 13

Multicolumn Thermostat G7116A 14

Diode Array Detector HS G7117C 15

Diode Array Detector WR G7115A 16

Variable Wavelength Detector G7114A 17

Fluorescence Detector G7121A 18

Refractive Index Detector G7162A 19

Solutions 20

Walk-up 20

Online SPE 20

1260 Infinity II Multimethod Solution 22

Optimizing the Stack Configuration 24

Agilent A-Line LC Flex Bench 24

One Stack Configuration 26

Two Stack Configuration 28

Leak and Waste Handling 30

Waste Guidance 34

Waste Concept 35

This chapter gives an introduction to the Agilent 1260 Infinity II LC, the

underlying concepts and the features of the Agilent 1260 Infinity II LC.

6 1260 Infinity II Quaternary System - Manual and Quick Guide

Introduction

Product Description

The Agilent 1260 Infinity II LC System is the trusted platform, taking you to

the next level of routine analysis, and giving you the instrument choice to

achieve best operational efficiency.

A broad range of reliable instrumentation matches with latest column

technologies guarantees robust separation and detection performance.

Highest instrument utilization and a fast turnaround cycle is achieves through

easy column handling and superior sample logistics from sample submission

to data analysis.

The designed for method transferability and stepwise upgrade capability

enables a risk-free integration in current infrastructure matching your budget.

1260 Infinity II Quaternary System - Manual and Quick Guide 7

Introduction

Features of the Agilent 1260 Infinity II Quaternary LC

With an operating pressure of up to 600 bar the 1260 Infinity II Quaternary

Pump is compatible with HPLC and UHPLC, i.e. handling 2.1, 3, and 4.6 mm ID

columns over the flow rate range (up to 5 mL/min) and semi preparative

analysis due to flow up to 10 mL/min.

Ultralow carryover – The 1260 Infinity II Multisampler is designed for low

carryover, you can take clean to a whole new level with the multi-wash

capability, cleaning all relevant injection parts between runs. This

sophisticated, integrated feature flushes the injection needle outside with

three solvents, and uses seat back flush procedures to reduce carryover to less

than 9 ppm.

Dual-needle injection – By running samples alternately through one or the

other injection path, you can reduce cycle times to mere seconds, virtually

eliminating conventional wait times—whether for large volume loadings or

flushing procedures.

Higher sample capacity per benchspace – Using shallow well-plate drawers,

the 1260 Infinity II Multisampler takes a maximum load of 16 microtiter plates

and up to 6144 samples—the most of any single system.

Advanced column capacity for up to 4 columns in a single 1260 Infinity II

Multicolumn Thermostat delivering best flexibility for column switching.

Fast and easy connections with A-line Quick Connect fittings to save time and

trouble.

1260 Infinity II DAD HS Detector delivers lower detection limit and higher

data quality for more confidence.

Seamless integration in your chromatography data system: Agilent’s

Instrument Control Framework (ICF) enables smooth control of Agilent LC

instrumentation through third-party chromatography data systems.

8 1260 Infinity II Quaternary System - Manual and Quick Guide

Introduction

System Components

The Agilent 1260 Infinity II LC System consists of the following components:

•Quaternary Pump or Isocratic Pump

•Multisampler or Vialsampler

•Multicolumn Thermostat (MCT)

•Diode Array (DAD), Variable Wavelength (VWD), Refractive Index (RID), or

Fluorescence Detector (FLD)

•Evaporative Light Scattering Detector (ELSD) (optional, not stackable)

•Solvent Cabinet

The Agilent 1260 Infinity II LC is described in more detail in the following

sections. All modules (except the optional ELSD) are stackable, see

“Optimizing the Stack Configuration” on page 24

For specifications, please refer to the individual module user documentation.

1260 Infinity II Quaternary System - Manual and Quick Guide 9

Introduction

System Components

Quaternary Pump G7111B

The Agilent 1260 Infinity II Quaternary Pump has an extended power range,

delivering pressures up to 600 bar. At these high pressures you can use

smaller particle size columns and get higher resolution and faster separations.

It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual

floating, precise servo-controlled pistons adjust the stroke volume according

to your chosen flow rate.

Offering access to up to four solvents, the Agilent 1260 Infinity II Quaternary

Pump provides the greatest flexibility in automated solvent blending and is

recommended for a wide range of research and routine applications

(especially in the food, environmental and pharmaceutical sectors) as well as

for method development.

Figure 1 Overview of the pump

6WDWXVLQGLFDWRU

3XUJHYDOYH

3RZHUVZLWFK

/HDNGUDLQ

'HJDVVHU

0&*9

10 1260 Infinity II Quaternary System - Manual and Quick Guide

Introduction

System Components

Quaternary Pump VL G7111A

The Agilent 1260 Infinity II Quaternary Pump VL has an extended power

range, delivering pressures up to 400 bar. At these high pressures you can use

smaller particle size columns and get higher resolution and faster separations.

It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual

floating, precise servo-controlled pistons adjust the stroke volume according

to your chosen flow rate.

Offering access to up to four solvents, the Agilent 1260 Infinity II Quaternary

Pump VL provides the greatest flexibility in automated solvent blending and is

recommended for a wide range of research and routine applications

(especially in the food, environmental and pharmaceutical sectors) as well as

for method development.

Figure 2 Overview of the pump

6WDWXVLQGLFDWRU

3XUJHYDOYH

3RZHUVZLWFK

/HDNGUDLQ

'HJDVVHU

0&*9

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