Agilent Technologies Agilent 1260 Infinity Manual de usuario

Agilent Technologies

Agilent 1260 Infinity

Binary LC

System User Guide

1260 Infinity Binary LC - System User Guide

Notices

2015

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1260 Infinity Binary LC - System User Guide 3

In This Guide...

This manual covers the Agilent 1260 Infinity Binary LC.

1 The Agilent 1260 Infinity Binary LC - Product Description

This chapter discusses the features of the 1260 Infinity Binary LC.

2 Introduction

This chapter gives an introduction to the Agilent 1260 Infinity Binary LC

and the underlying concepts.

3 Optimization of the Agilent 1260 Infinity Binary LC

This chapter considers how to apply the theory and use the features of

the LC system to develop optimized separations.

4 System Setup and Installation

This chapter includes information on software installation, installation of

the modules, and preparing the system for operation.

5QuickStartGuide

This chapter provides information on data acquisition and data analysis

with the 1260 Infinity Binary LC.

6 Appendix

This chapter provides additional information on safety, legal and web and

about setting up a method.

4 1260 Infinity Binary LC - System User Guide

Contents

1 The Agilent 1260 Infinity Binary LC - Product Description 7

Features of the Agilent 1260 Infinity Binary LC 8

System Components 10

Specifications 22

2 Introduction 27

Theory of Using Smaller Particles in Liquid Chromatography 28

Benefits of small particle size columns 34

Frictional Heating 37

3 Optimization of the Agilent 1260 Infinity Binary LC 39

How to Configure the Optimum Delay Volume 40

How to Achieve Higher Injection Volumes 47

How to Achieve Shorter Cycle Times 48

How to Achieve Lowest Carry-over 51

How to Achieve Higher Resolution 53

How to Achieve Higher Sensitivity 60

How to Prevent Column Blockages 65

4 System Setup and Installation 67

Installing Software 68

Installing the Modules 70

5QuickStartGuide 81

About the Quick Start Guide 82

Preparing the System 83

Data Acquisition in Method and Run Control View 88

Data Analysis 96

1260 Infinity Binary LC - System User Guide 5

Contents

6 Appendix 101

Safety Information 102

Solvent Information 105

Agilent Technologies on Internet 110

Setting Up a Method using Edit Entire Method 111

6 1260 Infinity Binary LC - System User Guide

Contents

1260 Infinity Binary LC - System User Guide

Agilent Technologies

The Agilent 1260 Infinity Binary LC -

Product Description

Features of the Agilent 1260 Infinity Binary LC 8

System Components 10

1260 Infinity Binary Pump (G1312B) 11

1260 Infinity High Performance Degasser (G4225A) 14

1260 Infinity High Performance Autosampler (G1367E) 15

1290 Infinity Thermostatted Column Compartment (G1316C) 17

1260 Infinity Diode Array Detector (G4212B) 19

1200 Infinity Series Quick Change Valves 21

Specifications 22

This chapter discusses the features of the 1260 Infinity Binary LC.

8 1260 Infinity Binary LC - System User Guide

1The Agilent 1260 Infinity Binary LC - Product Description

Features of the Agilent 1260 Infinity Binary LC

The design concept of the 1260 Infinity Binary LC is to provide a liquid

chromatograph offering ultra fast and high resolution separation capability

and yet which retains full functionality for standard HPLC applications.

Therefore, it provides full backwards compatibility for your established

HPLC and UHPLC methods. The use of sub- two micron (STM) particles

means that for high flowrates or long columns additional pressure is

required to drive the mobile phase through the column. The flowpath of

the 1260 Infinity Binary LC is optimized to produce minimal backpressure,

and ZORBAX RRHT columns have an engineered particle size distribution

that produces significantly less backpressure than other STM columns.

The design features and benefits of the Agilent 1260 Infinity Binary LC

are:

•The configurable delay volume down to 120 µL in the 1260 Infinity

Binary Pump combined with a flow range from 0.05 up to 5 mL/min at

pressures up to 600 bar provides universal applicability from narrow

bore (2.1 mm ID) to standard bore (4.6 mm ID) columns, matching the

needs for both LC and LC/MS.

•The standard delay volume configuration of the 1260 Infinity Binary

Pump allows you to run not only UHPLC but also conventional HPLC

methods without compromising performance or changing

chromatographic patterns.

•The next generation flow- through design of the 1260 Infinity High

Performance Autosampler achieves highest precision for a wide range of

injection volumes (from 0.1 up to 100 µL) without changing sample

loops. It is designed for high sample throughput, low carryover, and fast

injection cycles.

•High temperature, up to 100 °C on certain columns, allows more

selectivity flexibility and reduces solvent viscosity to allow even faster

separation.

•In the 1290 Infinity Thermostatted Column Compartment , different

heater (1.6 µL) and cooling elements (1.5 µL) for low extra- column

volume can be installed. The temperature is adjustable from 10 °C

below ambient up to 100 °C.

1260 Infinity Binary LC - System User Guide 9

The Agilent 1260 Infinity Binary LC - Product Description 1

Features of the Agilent 1260 Infinity Binary LC

•The new pull- out valve drive design and user- exchangeable

Quick- Change valves in the 1290 Infinity Thermostatted Column

Compartment boosts usability and paves the way for ultra

high- throughput, multi- method and automated method development

solutions.

•A low dispersion tubing kit and low volume flow cells minimize peak

dispersion for narrow bore columns.

•Baseline robustness and fast spectral acquisition at data rates up to

80 Hz through the new optical design of the 1260 Infinity Diode Array

Detector .

•Different UV detector flow cells for use with 2.1, 3.0 and 4.6 mm inner

diameter columns are available, including the revolutionary Agilent

Max- Light cartridge flow cell with 60 mm optical path length (typical

noise: <±0.6 µAU/cm) for ultra sensitivity in detection.

A stepwise upgrade from 1100 Series or 1200 Series to Agilent 1260

Infinity Binary LC is possible; for example a 1100 Series Detector or 1200

Series Column Compartment can be further used in combination with a

1260 Infinity Binary Pump.

10 1260 Infinity Binary LC - System User Guide

1The Agilent 1260 Infinity Binary LC - Product Description

System Components

Numerous system configurations of the 1260 Infinity Binary LC are

possible, tailored to the needs of your individual application requirements.

A few configurations are described in more detail in this manual (see

“System Setup and Installation” on page 67). The modules that are

described in the following sections are typical components of a 1260

Infinity Binary LC. In addition to these core components, individual

solutions are available for specific applications (some of which are

described in the Chapter "Optimization").

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