Agilent Technologies CrossLab RapidFire 400 Manual de usuario

RapidFire 400 System

Quick Start Guide

Where to find more information 2

User guides 2

Web Site 2

Getting Started 3

System Preparation 3

Run the RapidFire 400 System in Plates Mode 9

Run the RapidFire System in Sequences mode 11

RapidFire 400 System Flow Path 15

State 1: Aspirate 15

State 2: Wash/Load 16

State 3: Extra Wash 17

State 4: Elute 18

State 5: Re-equilibrate 19

Flush the sipper tube 20

Physical colors of the tubing 21

Valve positions 21

2 RapidFire 400 System Quick Start Guide

Where to find more information

User guides

You can access these guides from the RapidFire Resource App which you can

start from the RapidFire Resources icon.

User Guide This guide describes how to prepare, maintain, and run the

RapidFire 400 System.

Data Analysis Guide This guide describes how to use the RapidFire Integrator

software to prepare and analyze data.

Safety Guide This guides contains the safety instructions for operating the

RapidFire 400 System.

Web Site

Go to www.agilent.com for more information about the RapidFire System.

RapidFire 400 System Quick Start Guide 3

Getting Started

Refer to the RapidFire 400 System User Guide for detailed instructions.

System Preparation

System preparation

Step Details

1Prepare solvents Pull out the solvent drawer and make sure you have enough solvent.

2Check waste capacity Check that the waste containers have enough room to accommodate

the waste that will be generated from the runs that are planned.

3Start the vacuum system Open up the vacuum valve or switch on your standalone vacuum

pump.

4 RapidFire 400 System Quick Start Guide

4Start the software aDouble-click the RapidFire Control Panel desktop icon.

bClick File > Choose CFGs.

cSelect the configuration folder under C:\Agilent\RapidFire.

dClick Start in the Control Panel dialog box.

System preparation

Step Details

RapidFire 400 System Quick Start Guide 5

5Verify network settings aDouble-click the RapidFire UI desktop icon.

bClick System Tools > Network Settings.

cVerify that the IP addresses for the RapidFire computer and the

mass spectrometer computer are correct.

dClick Connect in the main window.

6Home the cartridge changer

and insert your cartridges

Click System Tools > Cartridge Changer. Refer to the User Guide for

more details.

System preparation

Step Details

6 RapidFire 400 System Quick Start Guide

7Home the stages aClick System Tools > Sipper Configuration.

bSelect the plate type you are using from the Plate to Configure list.

System preparation

Step Details

RapidFire 400 System Quick Start Guide 7

8Home the valves aClick System Tools > Valve Tuner to open the Valve Configuration

dialog box.

bClick Find to home a valve.

Refer to the User Guide for details.

9Prepare the peristaltic pump aCheck that Pump 4 is turned on and rotating clockwise.

bEnsure that the vacuum is at least -70 kPa.

System preparation

Step Details

8 RapidFire 400 System Quick Start Guide

10 Prepare the quaternary pumps aOpen the purge valves.

bTurn on the pump flow.

cTurn off the pump flow once the lines are purged.

dClose the purge valves.

11 Flush the sipper tube Under Sipper Settings, click Flush Now.

12 Prepare the MassHunter Data

Acquisition program

aIf you have two controlling computers (one for the mass spec (MS)

and one for RapidFire), on the MS computer, double-click on the

RapidFire Communicator desktop icon.

bStart the Data Acquisition program.

cTurn on the detector.

dCalibrate the mass spec.

eCreate or edit a method settings that are appropriate for your

analysis.

fSave the method.

System preparation

Step Details

RapidFire 400 System Quick Start Guide 9

Run the RapidFire 400 System in Plates Mode

Running the RapidFire 400 System in Plates Mode

Step Details

1Prepare the plates. Thaw sample plates and spin on a centrifuge at 3000 rpm for five minutes.

2Load the plates. Manually place a plate on the stage or load the stacks to use the plate

handler.

3Prepare the MassHunter

Data Acquisition program.

aOpen or create a method and set parameters for your analysis.

bSet up a Manual Run. Set injection volume to As Instrument.

cRun the sample.

10 RapidFire 400 System Quick Start Guide

4Set the RapidFire system

run parameters.

aSelect the Plates option.

bSet the parameters for your analysis in the RapidFire software main

window.

cSave the settings as a method.

5Start the run in Plates

mode.

Click the play button.

Running the RapidFire 400 System in Plates Mode

Step Details

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