Labnet ESCZ100A Manual de usuario

Rev 2 August 2016

Z100A Clinical Centrifuge

User Manual

ESCZ100A

About This Manual

This manual is designed to assist you in the optimal usage of your Z100A Clinical

Centrifuge.

Safety Precautions

NEVER use the centrifuge in any manner not specified in these instructions.

NEVER operate the centrifuge without a rotor properly attached to the shaft.

NEVER fill tubes while they are in the rotor. Liquid spillage may harm unit.

NEVER put hands in the rotor area unless the rotor is completely stopped.

NEVER move the centrifuge while the rotor is spinning.

NEVER use solvents or flammables near this or other electrical equipment.

NEVER centrifuge flammable, explosive or corrosive materials

NEVER centrifuge hazardous materials outside of a hood or proper containment facility

ALWAYS load the rotor symmetrically. Each tube should be counterbalanced by another

tube of the same type and weight

ALWAYS locate the centrifuge within easy access to an electrical outlet.

ALWAYS use only centrifuge tubes designed to withstand centrifugal forces of at least

1,967 x g.

ALWAYS use a wrench to tighten rotor nut.

Table of Contents

1. General Information 1

Description

Safety precautions to be observed before operating

Technical data

Accessories

Warranty

2. Installation 2

Unpacking the centrifuge

Required space

Cushion Installation

Centrifuge Installation

3. Operation 3

Closing the lid

Lid release

Lid lock

Starting the centrifuge

4. Proper use of the Rotor 3

Overloading the rotor

Balancing the rotor

Rotors and accessories

5. Service and Maintenance 4

Service of centrifuge

Cleaning the centrifuge

Disinfection

Cleaning the centrifuge after breakage of glass tubes or bottles

Replacing fuses

6. Troubleshooting guide 5

8. Determination of g-values 6

1. General Information

This manual is intended for use with the Z100-A (ESCZ100A) Centrifuge.

1.1 Description

The Z100-A is a small benchtop centrifuge designed for sedimentation of various

samples. The Z100-A has a built in angle rotor capable of running up to 4,000 rpm

(1,967 x g).

1.2 Safety precautions

Note: All users of the centrifuge must read the Safety Precautions section

of this manual before attempting to operate the unit!

If this equipment is used in a manner not specified by the manufacturer, the

protection provided by the equipment may be impaired.

Do not operate the centrifuge if any of the following conditions exist:

-The centrifuge has not been installed properly

-The centrifuge is partially dismantled

-Service has been attempted by unauthorized or unqualified personnel

-The rotor has not been installed securely on the motor shaft

-Rotors and accessories not belonging to the standard range are being used

without permission being obtained from the manufacturer to use such rotors

and/or accessories in the centrifuge

Exception: Centrifuge tubes, normally available in the laboratory.

-The centrifuge is located in an explosive atmosphere

-Materials to be centrifuged are combustible and/or explosive

-Materials to be centrifuged are chemically reactive

-The rotor load is not properly balanced

1.3 Technical data

Dimensions

Width 295mm (11.6in)

Depth 348mm (13.7in)

Height 101mm (10.1in)

Maximum speed 4,000rpm

Maximum RCF 1,967 x g

Maximum volume 6 x 15ml

Admiss. density 1.2kg/dm3

Rotor radius 11cm

Electrical/fuse rating 120V/50-60Hz, 1 Amp

1.4 Accessories supplied with centrifuge

- One 6 x 15mL rotor

-Six rubber cushions

Additional accessories available:

-Combination adapters for 5mL and 7mL tubes, (6pk)

1.5 Warranty

This centrifuge has been subject to thorough testing and quality control. In the unlikely

event of a manufacturing fault occurring, our one year warranty (from the date of

delivery) covers the centrifuge and the rotor. This warranty becomes invalid in the case

of incorrect operation, use of nonstandard spare parts or accessories and unauthorized

modification of the rotor or centrifuge.

Labnet reserves the right to make technical modifications. Statements contained herein

are not to be considered binding.

2. Installation

2.1 Unpacking the centrifuge

The centrifuge is delivered in a carton with two or more formed foam blocks. Please

retain the carton and the blocks until it has been established that your unit is working

properly.

The accessories supplied with the centrifuge should be kept with the instruction manual

near the centrifuge’s place of installation.

2.2 Required space

The centrifuge should be installed on a rigid, even surface such as a stable laboratory

bench, cabinet, etc. To guarantee sufficient ventilation, ensure that the centrifuge has

at least 15cm (6 inches) of free space on all sides, including the rear.

The centrifuge should not be located in areas subject to excessive heat such as in direct

sunlight or near radiators or the exhaust of a compressor, as a buildup of heat may

occur within the centrifuge bowl.

2.3 Cushion Installation

Before inserting any tubes into the centrifuge the rubber cushions must first be properly

installed. To install the cushions turn a 10 or 15mL round bottom tube upside down and

place the cushion on top. Then remove the rotor shield and place it on top of the tube

while pushing the cushion into the end of the shield. Then reinsert the shield.

2.4 Centrifuge Installation

Make certain that the time is set for the “off” position. Before operating the centrifuge,

check that the power source corresponds to that on the manufacturer’s rating label, then

connect the power cord to a properly grounded outlet.

Attention:

Ensure that the time is in the “OFF” position before connecting the power cord.

3. Operation

Attention:

Never operate the centrifuge if the rotor shows any signs of corrosion or mechanical

damage.

Never operate with strongly corrosive materials.

3.1 Closing the lid

After the rotor has been properly loaded, close the centrifuge lid.

3.2 Lid release

The “lid” button must be pushed to open the lid after a centrifuge run has been

completed.

3.3 Lid lock

The centrifuge can only be started with the lid closed. Do not attempt to open the lid

until the rotor comes to a complete stop.

3.4 Starting the centrifuge

To start the centrifuge select the desired run time by turning the time knob in a

clockwise direction. If performing a continuous run turn the time knob counter clockwise

into the “hold” position.

Once a run time is selected a given speed must be selected in order to start the

centrifuge.

The speed (rpm) can be selected between 1,000 and 4,000rpm by turning the knob to

the 1, 2, 3 and 4 speed marks. To select a speed, turn the speed knob to the 2 position

to activate, then proceed by turning the knob to the desired speed.

At the end of the preselected time, the centrifuge stops automatically. To stop the

centrifuge prior to the selected time, turn the timer knob to the “OFF” position.

Note: The clinical centrifuge is calibrated at 120 volts line voltage. Operating the

centrifuge at a line voltage other than 120V can significantly impact the speed settings

below 2,000 rpm.

4. Proper loading of the rotor

4.1 Rotor capacity

The rotor has been designed to handle samples with a maximum density of 1.2g/mL.

Tubes should not be run with any substance having a density above 1.2g/mL.

4.2 Rotor loading

When loading the rotor you should proceed according to sketch A. Fill the tubes equally

by eye and insert them into the tube shields of the rotor. The difference in weight

between the tubes should not exceed 2 – 3 grams.

A partially loaded rotor may be centrifuged. For example, you may operate a 6-place

rotor with 2, 3 or 4 loaded tubes, but it is important that the occupied tube shields are

symmetrically arranged. (see sketch A)

Sketch A: Examples of properly loaded rotors.

4.3 Rotors and accessories

Angle rotor for 6 x 10/15mL tubes (included)

Centrifuging radius 11cm

MaxRCF 1,967 x g

Adapter C0200-17A:

For 5mL (13 x 75mm) and 7mL tubes (13 x 100mm)

Centrifuging radius 8.65cm

MaxRCF 1,547 x g

5. Service and Maintenance

5.1 Service of centrifuge

There is no routine maintenance or service required on the centrifuge other than

occasional cleaning.

5.2 General cleaning of the centrifuge

Always keep the centrifuge housing, rotor chamber, rotor and rotor accessories clean.

All parts should be wiped down periodically with a soft cloth. Do not use harsh

Cleaners or detergants on the lid or housing surfaces as it may affect the plastic. After

cleaning, ensure that all parts are dried thoroughly by hand or in a warm-air-cabinet

(max. temp. +50ºC)

5.3 Disinfection

The centrifuge should be disinfected periodically and before shipping the unit for service.

The centrifuge rotor are and rotor shields should be wiped with alchohol or other

disinfecting agents. Do not allow the alcohol or disinfectant to sit on the units lid or

housing, as it may affect or discolor the plastic.

5.4 Cleaning the centrifuge after breakage of glass tubes or bottles

With high g-values, there is a possibility that tube breakage can occur. Should this

happen the centrifuge rotor must be thoroughly cleaned and all broken particles

removed. If this is not done, damage could result to the centrifuge. If glass or other

material has gotten into the centrifuge enclosure, the enclosure must be removed for

cleaning.

5.5 Replacing fuses

Check the fuse when it is recommended in the “Troubleshooting Guide” located in

this manual. The fuse holder is located underneath the unit. Unplug the unit. Unscrew

the fuse holder and remove the fuse.

Replace a blown fuse with a fuse of equivalent rating and type.

6. Troubleshooting Guide

Please refer to this guide before calling for service.

Centrifuge will not start

Possible reason: No power supply

Solution: Check main power supply and centrifuge fuse

Possible reason: Blown fuse

Solution: Check fuse and replace if necessary (see 5.5)

Lid lock will not release

Possible reason: Lid lock is jammed

Solution: Push front of lid down while pushing release button

Centrifuge cannot be started, although power is on

Possible reason: Lid not closed correctly

Solution Close lid correctly, push down on front of lid

Possible reason: No speed or time has been selected

Solution: Check and set speed and/or time

Possible reason: Blown fuse

Solution Check fuse and replace if necessary (see 5.5)

8. Determination of g-values

See section 4.3 for the correct radius of your rotor. Note the use of tube adapters will

change the radius.

To determine the relative centrifugal force (RCF-value).

This value is the multiplier to the gravitational pull of the earth (g).

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