Labnet BioPette A Series Manual de usuario

Labnet BioPette A

Single-channel, 8-channel,

and 12-channel Pipettors

Instruction Manual

This manual is available in additional languages

at www.labnetlink.com.

Table of Contents

1. Product Description ............................................................. 1

2. Packing........................................................................................ 2

3. Pipettor Design........................................................................ 2

4. Safety Recommendations .................................................. 3

 5. Specications........................................................................... 4

6. Pipettor Operation................................................................. 6

7. Operating Instructions ......................................................10

8. Aspiration and Dispensing Instructions.....................11

9. Checking Pipetting Accuracy Parameters

and Pipettor Recalibration................................................13

10. Pipettor Maintenance .......................................................16

11. Troubleshooting ...................................................................18

12. Spare Parts ..............................................................................20

13. Limited Warranty..................................................................23

Instruction Manual – 1

1. Product Description

The Labnet BioPette A single-channel pipettor is a volumetric

instrument designed to measure and transfer liquids

precisely and safely. It is available in volumes from 0.1 μL

to 10,000 μL.

The Labnet BioPette A 8-channel and 12-channel pipettors

aredesignedformicroplatelling.Thepipettorsenable

precise and simultaneous delivery of 8 or 12 preset

volumes of liquid. These pipettors are available in four

volume ranges: 0.5-10 μL, 5-50 μL, 20-200 μL, and 50-300 μL.

Nominal

Volume (µL) Cat. No.

Range of Pipettor

Volumes (µL)

Single-channel Pipettors

2 P3960-2A 0.1 - 2

10 P3960-10A 0.5 - 10

20 P3960-20A 2 - 20

100 P3960-100A 10 - 100

200 P3960-200A 20 - 200

1,000 P3960-1000A 100 - 1,000

5,000 P3960-5000A 1,000 - 5,000

10,000 P3960-10000A 1,000 - 10,000

8-channel and 12-channel Pipettors

8-10

12-10

P4608-10A

P4612-10A 0.5 - 10

8-50

12-50

P4608-50A

P4612-50A 5 - 50

8-200

12-200

P4608-200A

P4612-200A 20 - 200

8-300

12-300

P4608-300A

P4612-300A 50 - 300

Labnet pipettors operate using an air-cushion (i.e., the

aspirated liquid does not come into contact with the

shaft or plunger of the pipettor). The liquid is drawn into

the disposable tip attached to the pipettor.

2 – LABNET BIOPETTE A PIPETTORS

2. Packing

The pipettors are delivered with the following:

Description Qty/Pk

Instruction manual 1

QualityControlcerticate 1

Calibration key 1

Identicationlabels 8

Lubricant (for single-channel pipettor) 1

Ejector spacers (for models up to P3960-1000A) 2

Ejector cap (for P3960-5000A and P3960-10000A models) 1

Filters (for P3960-5000A and P3960-10000A models) 3

Filters

The 5,000 and 10,000 μL pipettors include a replaceable

lter,ttedatthebottomoftheshaftwhichprevents

aspirated liquid from entering the shaft and contaminating

theinnersurfaceandplunger.Useofthelterisparticularly

important when aspirating and dispensing large volumes

ofliquid.Theltershouldbereplacedifitbecomeswet.

3. Pipettor Design

Single- and Multi-channel Pipettors

Instruction Manual – 3

A. Pipetting pushbutton: Consists of 2 parts:

the pushbutton (A1) and the knob (A2).

B. Volume adjustment knob: Used to set the volume.

C. Shaft: Manufactured using high quality plastic,

ensuring high chemical and mechanical strength.

D. Tip ejector: For multi-channel pipettors, the tips are

ejected sequentially, reducing the required force.

F. Shaft nut

N. Ejector pushbutton

R. Multi-channel manifold: Contains a set of plungers

andasetofexible-suspensionshafts,whichfunction

to reduce the force required to attach the tip.

Model Identication

The volume range of the pipettor is shown on the

pipetting pushbutton.

4. Safety Recommendations

Long-term use of the pipettor relies on correct method of use.

Please read and follow the instructions for use carefully.

Symbols used:

Danger, risk of injury.

NOTE Risk of damage to the pipettor or errors in pipetting.

NOTE:

• The pipettor is designed for the transfer of liquids only

using the tip. Do not aspirate liquids without the tip

attached. The aspirated liquid should not enter the

pipettor, as it may cause damage.

• Single-use tips reduce the risk of contamination of

samples.

• Keep the pipettor clean, avoiding the use of abrasive or

corrosive cleaning agents (e.g., acetone).

• Keep the pipettor upright when there is liquid in the tip.

• Only using the pipettor in accordance with the

manufacturer’s instructions ensures the correct pipettor

parameters are maintained.

4 – LABNET BIOPETTE A PIPETTORS

• After replacing the plunger or the shaft, the pipettor

should be calibrated.

• In the case of incorrect operation, the device should

be cleaned in accordance with the Instruction Manual

or transferred to a service point.

• Ambient operating temperature is +5°C to 45°C.

• Ambient storage conditions (in the original packaging

during transport and short storage) is -25°C to 55°C.

When working with the pipettor:

• Follow general work safety regulations regarding

hazards related to work in the laboratory.

• Take special care when pipetting aggressive substances.

• Use appropriate protective attire (e.g., clothing, goggles,

and gloves).

• Avoid pointing the pipettor at yourself or others during use.

• Only use parts and accessories recommended by the

manufacturer.

5. Specications

The pipettor is a high quality instrument which offers

excellentaccuracyandprecision.Theaccuracyandprecision

(repeatability) of the liquid volume depends on the quality

of pipet tips used. The values for accuracy and precision

shown in the table below were obtained using manufacturer

non-lterpipettips.Thosetipsarerecommendedforuseto

ensure compatibility, accuracy and precision when pipetting.

Instruction Manual – 5

Nominal

Volume

(µL)

Volume

(µL)

Accuracy

(%)

Precision

(%)

Non-lter

Tips (µL)

Single-channel Pipettors

Min. 0.1 ±40.0 ≤12.0

0.2 ±12.0 ≤6.0

1 ±2.7 ≤1.3

Max. 2 ±1.5 ≤0.7

Min. 0.5 ±4.0 ≤2.8

1.0 ±2.5 ≤1.8

5.0 ±1.0 ≤0.6

Max. 10.0 ±0.5 ≤0.4

Min. 2 ±3.0 ≤1.5

20010 ±1.0 ≤0.5

Max. 20 ±0.8 ≤0.3

100

Min. 10 ±1.6 ≤0.80

20050 ±0.8 ≤0.24

Max. 100 ±0.8 ≤0.20

200

Min. 20 ±1.2 ≤0.60

200100 ±0.8 ≤0.25

Max. 200 ±0.6 ≤0.20

1,000

Min. 100 ±1.6 ≤0.40

1,000500 ±0.7 ≤0.20

Max. 1,000 ±0.6 ≤0.15

5,000

Min. 1,000 ±0.6 ≤0.25

5,0002,500 ±0.6 ≤0.20

Max. 5,000 ±0.5 ≤0.15

10,000

Min. 1,000 ±2.5 ≤0.6

10,0005,000 ±0.8 ≤0.3

Max. 10,000 ±0.5 ≤0.2

8-channel and 12-channel Pipettors

8 - 10

12 - 10

Min. 0.5 ±10.0 ≤8.0

1 ±8.0 ≤6.0

5 ±4.0 ≤2.0

Max. 10 ±2.0 ≤1.2

8 - 50

12 - 50

Min. 5 ±4.0 ≤2.5

20025 ±3.0 ≤1.2

Max. 50 ±1.6 ≤0.6

8 - 200

12 - 200

Min. 20 ±3.0 ≤1.5

200100 ±1.5 ≤0.8

Max. 200 ±1.0 ≤0.6

8 - 300

12 - 300

Min. 50 ±1.6 ≤1.5

300150 ±1.2 ≤1.0

Max. 300 ±1.0 ≤0.6

6 – LABNET BIOPETTE A PIPETTORS

The accuracy and precision were obtained gravimetrically

using manufacturer tips performing at least 10 measurements

of distilled water at a temperature of 20°C ± 1°C according to

EN ISO 8655 standards.

Theuseoftipsfromothermanufacturersorltertipsmay

result in incorrect liquid aspiration and require pipettor

recalibration.

The design of the pipettor enables the user to recalibrate it

according to the information presented in Section 9.

6. Pipettor Operation

Attaching the Tips

• Attach the correct tip corresponding to the model number

displayed below counter window (Section 5).

• Position the pipettor vertically when attaching tips.

• Single-channelpipettors:Pushthepipettiponrmly

using a slight twisting motion to ensure an airtight seal.

• Multi-channel pipettors: Press the pipettor against

thetipspositionedintherackboxuntiltheshaft

retractsapproximately1.5mmintothemanifold.The

suspension system ensures even and airtight sealing of

the pipet tips. The rocking motion does not have to be

performed to seal the pipet tips tightly.

✓✕ ✓✕

NOTE:

• Do not attach the tips with a rocking movement,

as this may damage the shaft or plunger. Observe this

rule particularly with single-channel pipettors of low

volume range.

• Never draw liquids directly into the pipettor without

the tip attached.

Instruction Manual – 7

Adjusting the tip ejector

The tip ejector can be adjusted by the user and can

accommodate most types of tips available on the market.

When using narrow tubes, it may be necessary to remove

the tip ejector.

2-1,000 µL pipettors

1. Tip ejector disassembly

• Press the tip ejector

pushbutton.

• Slide the ejector from the

arbor.

2. Tip ejector assembly

• Press the tip ejector

pushbutton.

• Line the metal tip ejector up

with the arbor.

• Slide the ejector on the

arbor.

3. Tip ejector adjustment

• Slide the ejector from the arbor.

• Remove “H0” spacer that is inserted on the ejector

arbor. To increase the ejector length use ”H” spacers,

which allow for regulating the length of tip ejector

by +1 mm (H1) or +2 mm (H2). The outside shape

identiesthespacers.

8 – LABNET BIOPETTE A PIPETTORS

5,000 and 10,000 µL pipettors

1. Tip ejector disassembly

• Remove the tip ejector pushbutton.

• Using a screwdriver, turn the metal arbor counter-

clockwise to unscrew the ejector from the arbor.

• Release the ejector.

2. Tip ejector assembly

• Remove the tip ejector pushbutton.

• Using a screwdriver, turn the metal arbor clockwise

to secure the ejector and adjust its length.

NOTE: Leave a space of at least 1 mm between the

shaft and tip ejector collar.

3. Tip ejector adjustment

• Remove the ejector pushbutton.

• Use the screwdriver to increase or decrease the

ejector length.

• Following ejector assembly or adjustment, ensure

the ejector pushbutton is relocated.

Ifthetipejectorcannotbeadjustedsufciently,orifthe

diameteroftheejectorisinsufcienttoejectthetip,it

may be necessary to attach the ejector cap “M”to the

ejector.

Este manual sirve para los siguientes modelos

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