Labnet BioPette PLUS Manual de usuario

Labnet BioPette Plus

Single-channel, 8-channel,

and 12-channel Pipettors

Instruction Manual

This manual is available in additional languages

at www.labnetlink.com.

Table of Contents

1. Product Description ............................................................... 1

2. Packing.........................................................................................2

3. Pipettor Design.........................................................................2

4. Safety Recommendations .................................................. 4

5. Specications ............................................................................5

6. Pipettor Operation ..................................................................7

7. Operating Instructions ....................................................... 11

8. Aspiration and Dispensing Instructions.......................12

9. Checking Pipetting Accuracy Parameters

and Pipettor Recalibration.................................................14

10. Pipettor Maintenance .........................................................18

11. Troubleshooting ....................................................................19

12. Spare Parts................................................................................21

13. Limited Warranty ..................................................................24

Instruction Manual – 1

1. Product Description

The BioPette Plus single-channel pipettor isavolumetric

instrument designed to measure and transfer liquids

precisely and safely. It is available in volumes from 0.1μL

to 10,000 μL.

The BioPette Plus 8-channel and 12-channel pipettors

are designed for microplate lling. Thepipettors enable

precise and simultaneous delivery of8 or 12 preset

volumes ofliquid. These pipettors are available in four

volume ranges: 0.5-10 μL, 5-50 μL, 20-200 μL, and 50-300 μL.

Nominal Volume

(µL) Cat. No.

Range of Pipettor

Volumes (µL)

Single-channel pipettors

2 P3942-2 0.1 - 2

10 P3942-10 0.5 - 10

20 P3942-20 2 - 20

100 P3942-100 10 - 100

200 P3942-200 20 - 200

1,000 P3942-1000 100 - 1,000

5,000 P3942-5000 1,000 - 5,000

10,000 P3942-10000 1,000 - 10,000

8-channel and 12-channel Pipettors

8-10

12-10

P4808-10

P4812-10 0.5 - 10

8-50

12-50

P4808-50

P4812-50 5 - 50

8-200

12-200

P4808-200

P4812-200 20 - 200

8-300

12-300

P4808-300

P4812-300 50 - 300

Labnet pipettors operate using an air-cushion (i.e.,the

aspirated liquid does not come into contact with the

shaft or plunger of the pipettor). The liquid is drawn into

thedisposable tip attached to the pipettor.

2 – LABNET BIOPETTE PLUS PIPETTORS

2. Packing

The pipettors are delivered with the following:

Description Qty/Pk

Instruction manual 1

Quality Control certicate 1

Calibration key 1

Pipettor hanger 1

Identication labels 1

Lubricant (for single-channel pipettor) 1

Ejector cap (for pipettor models P3942-2,

P3942-10, P3942-5000, P3942-10000) 1

Filters (for pipettor models

P3942-5000, P3942-10000) 3

Filters

The 5,000 μL and 10,000 μL pipettors include areplaceable

lter, tted at the bottom of the shaft which prevents

aspirated liquid from entering the shaft and contaminating

the inner surface and plunger. Use of the lter is particularly

important when aspirating and dispensing large volumes

ofliquid. The lter should be replaced if it becomes wet.

3. Pipettor Design

Model Identication

The volume range of the pipettor is shown on the

pushbutton and on the tip ejector bar in multi-channel

pipettors.

Instruction Manual – 3

Single- and Multi-channel Pipettors

A. Pipetting pushbutton: Consists of 2 parts:

thepushbutton (A1) and the knob (A2).

B1. Volume adjustment knob: Used to set thevolume.

B2. Locking Ring: Allows the volume setting to be locked by

pushing the ring upwards.

C. Shaft: Manufactured using high quality plastic,

ensuring high chemical and mechanical strength.

D. Tip ejector: For multi-channel pipettors, the tips are

ejected sequentially, reducing the required force.

F. Shaft nut

N. Ejector pushbutton

R. Multi-channel manifold: Contains a set of plungers

andaset of exible-suspension shafts, which function

to reduce the force required to attach the tip.

4 – LABNET BIOPETTE PLUS PIPETTORS

4. Safety Recommendations

Long-term use of the pipettor relies on correct method of use.

Please read and follow the instructions for use carefully.

Symbols used:

Danger, risk of injury.

NOTE Risk of damage to the pipettor or errors in pipetting.

NOTE:

• The pipettor is designed for the transfer of liquids only

using the tip. Do not aspirate liquids without the tip

attached. The aspirated liquid should not enter the

pipettor, asit may cause damage.

• Single-use tips reduce the risk of contamination of

samples.

• Keep the pipettor clean, avoiding the use of abrasive or

corrosive cleaning agents (e.g., acetone).

• Keep the pipettor upright when there is liquid in the tip.

• Only using the pipettor in accordance with the

manufacturer’s instructions ensures the correct pipettor

parameters are maintained.

• After replacing the plunger or the shaft, the pipettor

should be calibrated.

• In the case of incorrect operation, the device should

be cleaned in accordance with the Instructions for Use

ortransferred to a service point.

• Ambient operating temperature is +5°C to 45°C.

• Ambient storage conditions (in the original packaging

during transport and short storage) is -25°C to 55°C.

When working with the pipettor:

• Follow general work safety regulations regarding

hazards related to work in the laboratory.

• Take special care when pipetting aggressive substances.

• Use appropriate protective attire (e.g., clothing, goggles,

and gloves).

• Avoid pointing the pipettor at yourself or others during use.

• Only use parts and accessories recommended by the

manufacturer.

Instruction Manual – 5

5. Specications

The pipettor is a high quality instrument which oers

excellent accuracy and precision. The accuracy and precision

(repeatability) of the liquid volume depends on the quality

of pipet tips used. The values for accuracy and precision

shown in the table below were obtained using manufacturer

non-lter pipet tips. Those tips are recommended for use to

ensure compatibility, accuracy and precision when pipetting.

Nominal

Volume

(μL)

Volume

(μL)

Accuracy

(%)

Precision

(%)

Non-lter

Tips (μL)

Single-channel Pipettors

Min. 0.1 ±40.0 ≤12.0

0.2 ±12.0 ≤6.0

1 ±2.7 ≤1.3

Max. 2 ±1.5 ≤0.7

Min. 0.5 ±4.0 ≤2.8

1.0 ±2.5 ≤1.8

5.0 ±1.0 ≤0.6

Max. 10.0 ±0.5 ≤0.4

Min. 2 ±3.0 ≤1.5

20010 ±1.0 ≤0.5

Max. 20 ±0.8 ≤0.3

100

Min. 10 ±1.6 ≤0.80

20050 ±0.8 ≤0.24

Max. 100 ±0.8 ≤0.20

200

Min. 20 ±1.2 ≤0.60

200100 ±0.8 ≤0.25

Max. 200 ±0.6 ≤0.20

1,000

Min. 100 ±1.6 ≤0.40

1,000500 ±0.7 ≤0.20

Max. 1,000 ±0.6 ≤0.15

5,000

Min. 1,000 ±0.6 ≤0.25

5,0002,500 ±0.6 ≤0.20

Max. 5,000 ±0.5 ≤0.15

10,000

Min. 1,000 ±2.5 ≤0.6

10,0005,000 ±0.8 ≤0.3

Max. 10,000 ±0.5 ≤0.2

6 – LABNET BIOPETTE PLUS PIPETTORS

Nominal

Volume

(μL)

Volume

(μL)

Accuracy

(%)

Precision

(%)

Non-lter

Tips (μL)

8-channel and 12-channel Pipettors

8 - 10

12 - 10

Min. 0.5 ±10.0 ≤8.0

1 ±8.0 ≤6.0

5 ±4.0 ≤2.0

Max. 10 ±2.0 ≤1.2

8 - 50

12 - 50

Min. 5 ±4.0 ≤2.5

20025 ±3.0 ≤1.2

Max. 50 ±1.6 ≤0.6

8 - 200

12 - 200

Min. 20 ±3.0 ≤1.5

200100 ±1.5 ≤0.8

Max. 200 ±1.0 ≤0.6

8 - 300

12 - 300

Min. 50 ±1.6 ≤1.5

300150 ±1.2 ≤1.0

Max. 300 ±1.0 ≤0.6

The accuracy and precision were obtained gravimetrically

using manufacturer tips performing at least 10measurements

ofdistilled water at a temperature of 20°C ± 1°C according to

ENISO 8655 standards.

The use of tips from other manufacturers or lter tips may

result in incorrect liquid aspiration and require pipettor

recalibration.

The design of the pipettor enables the user to recalibrate it

according to the information presented in Section 9.

Instruction Manual – 7

6. Pipettor Operation

Attaching the Tips

• Attach the correct tip corresponding to the model number

displayed on the pipettor pushbutton (Section5).

• Position the pipettor vertically when attaching tips.

• Single-channel pipettors: push the pipet tip on rmly

using a slight twisting motion to ensure an airtight seal.

• Multi-channel pipettors: press the pipettor against the

tips positioned in the rack box until the shaft retracts

approximately 1.5mm into the manifold. The suspension

system ensured even and airtight sealing of the pipet tips.

The rocking motion does not have to be performed to seal

the pipet tips tightly.

✓✕ ✓✕

NOTE:

• Do not attach the tips with a rocking movement,

as this may damage the shaft or plunger. Observe this

rule particularly with single-channel pipettors of low

volume range.

• Never draw liquids directly into the pipettor without

thetip attached.

Adjusting the tip ejector

The tip ejector can be adjusted by the user and can

accommodate most types of tip available on the market.

When using narrow tubes, it may be necessary to remove

the tip ejector.

8 – LABNET BIOPETTE PLUS PIPETTORS

2-1,000 μL pipettors

1. Tip ejector disassembly

• Press the tip ejector pushbutton.

• Turn the tip ejector bush 1/3 turn counter-clockwise.

• Slide the ejector from the plastic arbor.

2. Tip ejector assembly

• Press the tip ejector pushbutton.

• Line the tip ejector up with the plastic arbor.

• Turn the tip ejector bush clockwise until it has fully

latched.

3. Tip ejector adjustment

• Remove the tip ejector pushbutton.

• To increase the ejector length turn the plastic bush

counter-clockwise.

• To decrease the ejector length turn the plastic bush

clockwise.

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