Labnet Spectrafuge 16M Manual de usuario

Lit Code: M00001 Rev 2 August 2016

Spectrafuge 16M Microcentrifuge

User Manual

C0160

C0160-100V

C0160-230V

About This Manual

This manual is designed to assist you in the optimal usage of your Labnet 16M

Microcentrifuge. The manual is available in English, French, German, Italian,

Portuguese, and Spanish on our website at:

www.labnetinternational.com/document-center

Safety Precautions

NEVER use the centrifuge in any manner not specified in these instructions.

NEVER operate the centrifuge without a rotor properly attached to the shaft.

NEVER fill tubes while they are in the rotor. Liquid spillage may harm unit.

NEVER put hands in the rotor area unless the rotor is completely stopped.

NEVER move the centrifuge while the rotor is spinning.

NEVER use solvents or flammables near this or other electrical equipment.

NEVER centrifuge flammable, explosive or corrosive materials

NEVER centrifuge hazardous materials outside of a hood or proper containment

facility

ALWAYS load the rotor symmetrically. Each tube should be counterbalanced by

another tube of the same type and weight

ALWAYS locate the centrifuge within easy access to an electrical outlet.

ALWAYS use only microcentrifuge tubes made from plastic and designed to

withstand centrifugal forces of at least 16,000 x g.

Table of Contents

General Information 1

Description

Safety Precautions

Technical Data

Accessories supplied with unit

Installation 2

Unpacking the centrifuge

Required space

Installing the centrifuge

Rotor and Rotor Maintenance 2

Rotors and accessories

Rotor maintenance

Mounting and securing the angle rotors

Removing the rotor

Overloading rotors

Operation 5

Closing the lid

Lid release

Lid lock

Speed selection

Selection of operating time and momentary operation

Service and Maintenance 7

Service

Cleaning

Disinfection

Replacing fuses

Troubleshooting 8

Where to call

Determination of g-values

Relative Centrifugal Force 9

Symbols and Conventions 10

Warranty 11

General Information:

This manual provides important safety information for the Spectrafuge 16M

laboratory microcentrifuge. It should be kept near the centrifuge for quick and

easy reference.

Description

The Spectrafuge 16M is a small benchtop centrifuge designed for separation of

various research samples. The motor is brushless and requires no routine

maintenance. The Spectrafuge 16M is supplied with an 18 x 1.5ml rotor for

micro samples. Adapters are available for tubes smaller than 1.5ml. The

Spectrafuge 16M reaches speeds of up to 14,000rpm/16,000 x g.

Safety precautions

Note: All users of the centrifuge must read the Safety Precautions section of this

manual before attempting to operate the unit!

If this equipment is used in a manner not specified by the

manufacturer, the protection provided by the equipment may be

impaired.

Do not operate the centrifuge if any of the following conditions exist:

-The centrifuge has not been installed properly

-The centrifuge is partially dismantled

-Service has been attempted by unauthorized or unqualified personnel

-The rotor has not been installed securely on the motor shaft

-Rotors and accessories not belonging to the standard range are being used

without permission being obtained from the manufacturer to use such rotors

and/or accessories in the centrifuge

Exception: Microcentrifuge tubes made of plastic, normally available in the

laboratory.

-The centrifuge is located in an explosive atmosphere

-Materials to be centrifuged are combustible and/or explosive

-Materials to be centrifuged are chemically reactive

-The rotor load is not properly balanced

Technical data

Dimensions

Width 8.25 inches

Depth 8.9 inches

Height 7.6 inches

Maximum speed 14,000rpm

Maximum RCF 16,000 x g

Maximum volume 18 x 1.5/2.0ml

Admiss. density 1.2kg/dm3

Electrical/fuse rating 120V~, 50-60Hz, 1.0A/2.5AT

230V~, 50-60Hz, 0.6A/1.25AT

Accessories supplied with centrifuge

Each unit is supplied with 1 instruction manual and 1 power cord. Some models

are supplied with a rotor screw wrench.

Installation

Unpacking the centrifuge

Before unpacking the centrifuge, inspect the outside of the carton for any

shipping damage. The centrifuge is delivered in a carton with protective

cushions. Remove the centrifuge from the carton. Retain the carton and cushions

until it has been established that the centrifuge is working properly. Inspect the

centrifuge for any visible signs of shipping damage. Shipping damage is the

responsibility of the transportation carrier. Any claims for damage must be filed

within 48 hours. The accessories supplied with the centrifuge should be kept with

the instruction manual near the centrifuge’s place of installation.

Required space

The centrifuge should be installed on a rigid, even surface such as a stable

laboratory bench, cabinet, etc. To guarantee sufficient ventilation, ensure that the

centrifuge has at least 15cm (6 inches) of free space on all sides, including the

rear. The centrifuge should not be located in areas subject to excessive heat

such as in direct sunlight or near radiators or the exhaust of a compressor, as a

buildup of heat may occur within the chamber.

Installation

Make certain that the timer is set to the off position. Before operating the

centrifuge, check that the power source corresponds to that on the

manufacturer’s rating label, then connect the power cord to the centrifuge and

the power source.

ATTENTION: The timer must be in the OFF position before connecting

the power cord. Failing to place the timer in the off position may result in

damage to the centrifuge and injury to personnel.

Rotors and rotor maintenance

Rotors and accessories

The following accessories are available for the Spectrafuge 16M :

Angle rotor for 18 x 1.5ml tubes

Order no. Included with unit

Tube measurement 1.5ml (10 x 40mm)

Max. speed 14,000rpm

Centrifuging radius 7.3cm

RCF (g-value) 16,000 x g

Adapter for 0.5ml tubes

Order no. C1205

Tube measurement 8 x 30mm

Max. speed 14,000rpm

Centrifuging radius 6.6cm

RCF (g-value) 14,462 x g

Adapter for 0.4ml tubes

Order no. C1206

Tube measurement 6 x 47mm

Max. speed 14,000rpm

Centrifuging radius 7.3cm

RCF (g-value) 16,000 x g

Adapter for 0.2ml tubes

Order no. C1222

Tube measurement 6 x 21mm

Max. speed 14,000rpm

Centrifuging radius 6.1cm

RCF (g-value) 13,366 x g

Rotor maintenance

The rotor should be cleaned thoroughly after each use. Thorough cleaning must

be performed when spinning samples containing phenol or phenol chloroform.

Periodically inspect the rotor for dents, dings, scratches, discoloration and cracks.

If any damage to the rotor is found, discontinue use of the rotor immediately and

replace.

Mounting and securing the angle rotor

Remove the rotor screw from the motor shaft by turning the screw

counterclockwise. Clean the motor shaft and the rotor mounting hole (see figures

1&2). Place the rotor on the motor shaft ensuring that the cross-pin (figure 1)

aligns correctly with the rotor slot (see figure 2). Note: Figures 1 and 2 are

located on the following page. Reinstall the rotor screw on the motor shaft by

turning it clockwise. Hold the rotor with one hand and hand-tighten the rotor

screw. Use an adjustable or 1/4 inch wrench (some units are supplied with a

wrench) to tighten the screw When loading the rotor, refer to figure 3 (located on

page 5). Loading in the pattern indicated will ensure a balanced load. Tubes to

be loaded should be filled equally by eye. The difference in the weight between

the tubes should not exceed 0.1 gram. A partially loaded rotor may be

centrifuged if the loading scheme for balancing a rotor given in figure 3 is

followed.

Figure 1. Chamber and motor shaft

Figure 2. Bottom of angle rotor

Figure 3. Loading the rotor

Removing the rotor

Using an adjustable or 1/4 inch wrench (some units are supplied with a wrench)

loosen the screw and remove the rotor retaining screw/washer assembly by

turning it counterclockwise. Lift the rotor directly upward in a straight vertical

motion.

Caution: Be sure to secure the rotor screw and tighten with a wrench before

further operation.

Overloading rotors

The maximum load of the rotor and the maximum speed have been established

by the manufacturer. Do not attempt to exceed these values. The maximum

speed of the rotor has been measured for liquids having a homogeneous density

of 1.2g/ml or less. In order to centrifuge liquids with a higher density it is

necessary to reduce the speed. Failure to reduce the speed may result in

damage to the rotor and centrifuge. The revised maximum speed can be

calculated with the following formula:

Reduced speed (nred) x max speed (nmax)

Example:

Where the density of the liquid is 1.7, the new maximum speed would be

calculated as follows:

nred = x 14,000 = 9,882 rpm

If in doubt concerning maximum speeds, please contact the manufacturer for

assistance.

Operation

ATTENTION: Never attempt to operate the centrifuge with rotors or

adapters that show signs of corrosion or mechanical damage.

Never centrifuge strongly corrosive materials that may damage the

rotors or accessories.

Closing the lid

After the rotor has been properly secured and loaded, close the centrifuge lid,

making sure that the interlock has been engaged.

Figure 4. Spectrafuge 16M Control panel layout

Lid release

Once the run has been completed and the rotor come to a stop, the lid will open

automatically. If the lid does not open automatically, press the lid button. Note

that the lid button will not operate while the rotor is spinning.

WARNING: Do not attempt to open the lid of any centrifuge until the rotor

has come to a complete stop.

In the event of a power failure or malfunction, it may be necessary to open the lid

manually.

1. Disconnect the power cord from the wall socket.

2. Remove the plastic plug, located on the left side of the unit, below the quick

button.

3. Pull the cord (attached to the plug) to open the lid lock manually.

Lid lock

The centrifuge can be started only with the lid securely closed. When the rotor

begins to accelerate, the lid lock indicator light turns on and the lid button

becomes inoperable. Do not attempt to open the lid until the lid lock indicator

turns off. At the end of the run, the lid will automatically open.

Speed selection

The speed (rpm) can be selected to 14,000rpm with the knob labeled “speed”.

The scale is directly proportional to the speed - a setting of 9 corresponds to

9,000rpm, a setting of 13 corresponds to a speed of 13,000rpm, etc.

Selection of operating time and momentary operation

Operation of the centrifuge begins when the timer knob is turned clockwise to set

a run time. For run times less than 5 minutes, turn the knob clockwise past the

halfway point and then counterclockwise to the desired time. For run times longer

than 5 minutes, turn the knob clockwise to the desired time.

When the preselected time expires, the centrifuge will stop automatically. To stop

the centrifuge prior to the expiration of set time, turn the timer knob to the zero

position.

The centrifuge may be operated manually by pressing and holding the quick

button. The centrifuge will continue to run as long as the button is depressed.

Some models are equipped with a timer that includes a hold position. Continuous

operation of these models may be achieved by turning the timer knob firmly to

the left. The centrifuge will continue to operate until the knob is turned to the zero

position.

Note: The timer knob may be turned in either direction during operation of the

centrifuge without damage to the timer mechanism.

Service and Maintenance

Centrifuge service

The brushless motor in the Spectrafuge 16M requires no routine maintenance.

Any required service should be performed by authorized, qualified personnel only.

Repairs performed by unauthorized personnel may void the warranty.

Cleaning the centrifuge

Always keep the centrifuge housing, rotor chamber, rotor and rotor accessories

clean. All parts should be wiped down periodically with a soft cloth. For more

thorough cleaning, use a neutral cleaning agent (pH between 6 and 8) applied

with a soft cloth. Excessive amounts of liquid should be avoided. Liquid should

not come into contact with the motor. After cleaning, ensure that all parts are

dried thoroughly by hand or in a warm air cabinet (maximum temperature 50ºC).

Cleaning the rotor

The rotor should be cleaned after each use. When spinning 8 samples containing

phenol or phenol chloroform, the rotor should be cleaned immediately after use.

Disinfection

Should a spill of infectious materials occur within the rotor or chamber, the unit

should be disinfected. This should be performed by qualified personnel with

proper protective equipment.

Replacing fuses

Check the fuse when it is recommended in the Troubleshooting Guide located in

this manual. The fuse holder is located in the power inlet on the rear of the unit.

Disconnect the power cord from the power inlet. Open the fuse holder drawer by

inserting a small screwdriver under the tab and prying it open. Remove the

innermost (operative) fuse from its retaining tabs and replace the fuse if

necessary. A spare fuse is located in the outermost chamber of the fuse drawer.

Replace only with a fuse of exactly the same value as the original. (Fuse type

may be found in the Technical data section of this manual.)

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