Labnet Labpette FX Manual de usuario

Labnet Labpette FX

Single-channel, Fixed

Volume Pipettors

Instruction Manual

This manual is available in additional languages

at www.labnetlink.com.

Table of Contents

1. Product Description ...................................................................1

2. Packing..............................................................................................2

3. Pipettor Design.............................................................................2

4. Safety Recommendations ......................................................3

 5.Specications ................................................................................4

6. Pipettor Operation ......................................................................5

7. Operating Instructions .............................................................6

8. Aspiration and Dispensing Instructions...........................7

9. Checking Pipetting Accuracy Parameters

and Pipettor Recalibration.......................................................8

10. Pipettor Maintenance ........................................................... 12

11. Troubleshooting ....................................................................... 13

12. Spare Parts................................................................................... 15

13. Limited Warranty...................................................................... 17

Instruction Manual – 1

1. Product Description

LabnetLabpetteFXsingle-channel,xedvolumepipettoris

a volumetric instrument designed to measure and transfer

liquids precisely and safely. It can accommodate volumes

from 2 μL to 1,000 μL depending on the model.

Nominal Volume (µL) Cat. No. Color Code

2P3900-2 Red

5P3900-5 Red

10 P3900-10 Red

20 P3900-20 Yellow

40 P3900-40 Yellow

50 P3900-50 Yellow

70 P3900-70 Yellow

75 P3900-75 Yellow

100 P3900-100 Yellow

120 P3900-120 Yellow

150 P3900-150 Yellow

200 P3900-200 Yellow

250 P3900-250 Green

300 P3900-300 Green

400 P3900-400 Green

500 P3900-500 Green

1,000 P3900-1000 Green

Labnet pipettors operate using an air-cushion (i.e., the

aspirated liquid does not come into contact with the shaft

or plunger of the pipettor). The liquid is drawn into the

disposable tip attached to the pipettor.

2 – LABNET LABPETTE FX PIPETTORS

2. Packing

The pipettors are delivered with the following:

Description Qty/Pk

Instruction manual 1

Qualitycontrolcerticate 1

Calibration key 1

Shelf clip 1

Lubricant 1

3. Pipettor Design

Model Identication

The volume of the pipettor is shown on its pushbutton.

Pipetting pushbutton (A)

Ejector pushbutton (N)

Handle (O)

Tip ejector (D)

Shaft (C)

Instruction Manual – 3

4. Safety Recommendations

Long-term use of the pipettor relies on correct method of use.

Please read and follow the instructions for use carefully.

Symbols used:

Danger, risk of injury.

NOTE Risk of damage to the pipettor or errors in pipetting.

NOTE:

• The pipettor is designed for the transfer of liquids only

using the tip. Do not aspirate liquids without the tip

attached. The aspirated liquid should not enter the

pipettor, as it may cause damage.

• Single-use tips reduce the risk of contamination of

samples.

• Keep the pipettor clean, avoiding the use of abrasive or

corrosive cleaning agents (e.g., acetone).

• Keep the pipettor upright when there is liquid in the tip.

• Only using the pipettor in accordance with the

manufacturer’s instructions ensures the correct pipettor

parameters are maintained.

• After replacing the plunger or the shaft, the pipettor

should be calibrated.

• In the case of incorrect operation, the device should

be cleaned in accordance with the Instructions for Use

or transferred to a service point.

• Ambient operating temperature is +5°C to 45°C.

• Ambient storage conditions (in the original packaging

during transport and short storage) is -25°C to 55°C.

When working with the pipettor:

• Follow general work safety regulations regarding hazards

related to work in the laboratory.

• Take special care when pipetting aggressive substances.

• Use appropriate protective attire (e.g., clothing, goggles,

and gloves).

• Avoid pointing the pipettor at yourself or others during use.

• Only use parts and accessories recommended by the

manufacturer.

4 – LABNET LABPETTE FX PIPETTORS

5. Specications

The pipettor is a high quality instrument which offers

excellent accuracy and precision. The accuracy and precision

(repeatability) of the liquid volume depends on the quality

of pipet tips used. The values for accuracy and precision

shown in the table below were obtained using manufacturer

non-lterpipettips.Thosetipsarerecommendedforuseto

ensure compatibility, accuracy and precision when pipetting.

Nominal

Volume (µL) Cat. No.

Accuracy

(%)

Precision

(%)

Non-lter

Tips (µL)

2P3900-2 ±3.0 ≤1.5 200

5P3900-5 ±2.0 ≤1.0 200

10 P3900-10 ±1.2 ≤0.5 200

20 P3900-20 ±0.9 ≤0.4 200

40 P3900-40 ±0.9 ≤0.3 200

50 P3900-50 ±0.9 ≤0.3 200

70 P3900-70 ±0.9 ≤0.3 200

75 P3900-75 ±0.9 ≤0.3 200

100 P3900-100 ±0.9 ≤0.3 200

120 P3900-120 ±0.8 ≤0.3 200

150 P3900-150 ±0.7 ≤0.3 200

200 P3900-200 ±0.6 ≤0.2 200

250 P3900-250 ±0.6 ≤0.2 1,000

300 P3900-300 ±0.6 ≤0.2 1,000

400 P3900-400 ±0.6 ≤0.2 1,000

500 P3900-500 ±0.6 ≤0.2 1,000

1,000 P3900-1000 ±0.6 ≤0.2 1,000

The accuracy and precision were obtained gravimetrically

using manufacturer tips performing at least 10 measurements

of distilled water at a temperature of 20°C ± 1°C according to

EN ISO 8655 standards.

Theuseoftipsfromothermanufacturersorltertipsmay

result in incorrect liquid aspiration and require pipettor

recalibration.

The design of the pipettor enables the user to recalibrate it

according to the information presented in Section 9.

Instruction Manual – 5

6. Pipettor Operation

Attaching the Tips

• Attach the correct tip corresponding to the

model number displayed on the pipettor

ngerrest(Section5).

• Position the pipettor vertically when

attaching tips.

• Press the pipettor against the tip positioned

in the rack box. The suspension system

will ensure even and airtight sealing of the

pipet tips.

• Hold down the tip ejector button in order to

ensureatightertofthetip.

NOTE:

• Do not attach the tips with a rocking movement, as this

may damage the shaft or plunger.

• Never draw liquids directly into the pipettor without the

tip attached.

Ejecting the Tip

• Place your thumb against the ejector

pushbutton side and press it to the side.

6 – LABNET LABPETTE FX PIPETTORS

7. Operating Instructions

Observing the following recommendations will ensure

maximum possible accuracy and precision of liquid sampling.

• During operation, the volume setting should be locked,

with black adjustment knob in the lower position.

• Ensure smooth and slow operation of the pipettor.

• Immersion of the tip into the sample liquid should be

kept to a minimum depth, which should remain constant

during aspiration. The recommended immersion depths

are given in the table below:

Model Volume Range (µL) Immersion Depth (mm)

0.1-1 ≤1

1-100 2-3

101-1,000 2-4

• The pipettor should be held in a vertical position.

• The pipet tip should be changed whenever the volume

setting is altered, and when a different liquid is to be

aspirated.

• The pipet tip should be changed if a droplet remains on

the end of the tip from the previous pipetting operation.

• Each new pipet tip should be pre-rinsed with the liquid to

be pipetted.

• Liquid should never enter the pipettor shaft. To prevent this:

– Press and release the pushbutton slowly and smoothly.

– Never turn the pipettor upside down.

– Never lay the pipettor on its side when there is liquid in

the tip.

• Never force the volume setting beyond the recommended

limits.

• When pipetting liquids with a different temperature from

the ambient temperature, it is recommended to pre-rinse

the tip several times prior to use.

• Do not pipettor liquids with temperatures above 70°C.

NOTE: When pipetting acids or corrosive solutions which

emit vapors, it is recommended to disassemble the shaft

and rinse the plunger and O-ring with distilled water after

nishingthepipettingoperation.

Instruction Manual – 7

8. Aspiration and Dispensing Instructions

1. 2. 3. 5.4. 6.

Aspirating Liquid

1. Pressthepushbuttontotherststoppoint.

Holding the pipettor vertically, immerse the pipet tip

into the sample liquid to the recommended depth (for

recommended values see Section 7). If the pipet tip

is not immersed to the recommended depth or if the

pipetting pushbutton is rapidly released, air may enter

the pipet tip.

2. Release the pipetting pushbutton slowly and smoothly to

aspirate the sample. Wait one second and then withdraw

the pipet tip from the liquid.

CAUTION: Do not touch the used tip.

Dispensing Liquid

3. Place the end of the pipet tip against the inside wall of

the vessel at an angle of 10° to 40°. Press the pushbutton

smoothlytotherststop.Waitonesecond.

4. Press the pushbutton to the second stop to expel

any remaining liquid. While keeping the pushbutton

depressed, remove the pipettor from the vessel by

drawing the pipet tip against the inside surface of

the vessel.

5. Release the pushbutton to its starting position.

6. Eject the pipet tip by pressing the tip ejector pushbutton

to the side (left or right).

Remember to change the pipet tip whenever a different

type of liquid is to be sampled.

8 – LABNET LABPETTE FX PIPETTORS

Aspirating High-density Liquids

When pipetting liquids of higher viscosity or lower surface

tensionthanwater(e.g.,seraororganicsolvents),almof

liquid may be formed on the inside of the pipet tip which

mayproduceerroneousresults.Asthelmremainsrelatively

constant in successive pipetting operations with the same

tip, this error can be eliminated by pre-rinsing the tip and

allowingalmtoformbeforetransferringtherstsample.

This is achieved by aspirating a sample and dispensing it

backintothesamevessel.Allowingalmtoformpriorto

sampling ensures optimal accuracy and repeatability.

This pre-rinsing operation should be repeated when

the volume to be aspirated is changed or when a new pipet

tip is used.

NOTE: Normally the degree of error resulting from viscous

liquids is negligible if pipetting is performed slowly and

carefully, however can be minimized further by holding the

pipet tip in position for at least 2 seconds after aspiration

to allow the liquid time to react to the change in pressure

before it is dispensed.

If the above method does not result in accurate values,

recalibrate the pipettor in accordance with Section 9.

It is recommended to record recalibration and correction

values, in order to facilitate reverse calibration to a standard

liquid.

9. Checking Pipetting Accuracy Parameters

and Pipettor Recalibration

The pipettors have been factory-calibrated using gravimetric

methods with manufacturer pipet tips and distilled water,

in accordance with ISO 8655 guidelines

for the liquid volume drawn by the pipettor according to

the values given (Section 5). The pipettors are designed to

enable recalibration and adaptation to different pipetting

techniques and liquid properties (e.g., temperature, density,

and viscosity). Periodic checks of the operation of the

pipettor are recommended at least once per year. Frequency

of checks should be increased depending on workload,

sterilization or autoclave processes, and frequency of

replacement of component parts.

If during pipettor operation the accuracy error exceeds the

permissible value given in the table in Section 5, pipettor

recalibration should be carried out. Recalibration of the

pipettor involves adjusting the settings according to the

table on page 10.

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