Labnet MultiGene Mini Manual de usuario

MultiGeneMini

ThermalCycler

TC020-24 /TC020-24-230V

TC050-18 /TC050-18-230V

Operation Manual

Ver.1.2

Labnet International

CONTENTS

CHAPTER 1 SPECIFICATIONS ...................................................................................................... 1

1 Normal Operating Conditions .................................................................................................. 1

2 Transportation and Storage Conditions................................................................................... 1

3 Basic Specifications................................................................................................................. 1

4 Performance ............................................................................................................................ 2

5 Software Functions .................................................................................................................. 2

CHAPTER 2 Features ........................................................................................................................ 3

1 Description ............................................................................................................................... 3

2 Keyboard Diagram................................................................................................................... 4

3 Key Functions .......................................................................................................................... 4

4 Inspection before Power-up..................................................................................................... 5

5 Power-on Procedures .............................................................................................................. 5

CHAPTER 3 OPERATION GUIDE..................................................................................................... 6

1 How to Run a Program ............................................................................................................ 6

1.1 How to edit a program....................................................................................................... 7

1.2 How to Set System Parameters...................................................................................... 12

CHAPTER 4 TROUBLE SHOOTING............................................................................................... 14

1 Failure Analysis and Processing Procedures........................................................................ 14

2 Block Error Messages ........................................................................................................... 15

APPENDIX 1 Wiring Diagrams .................................................................................................... 16

APPENDIX 2 Parameter Values.................................................................................................. 18

CHAPTER 1 SPECIFICATIONS

This chapter describes the MultiGene Mini Thermal Cycler’s operation, transportation

and storage conditions, basic parameters, performance and functions.

1 Normal Operating Conditions

Ambient operating temperature: 10°C ∼30°C

Relative humidity: ≤70%

Power supply: ~100-120V, 200W, 50/60Hz

~220-240V, 200W, 50/60Hz

Note:

Before powering on, please confirm that the above operating

conditions are satisfied. The unit must be plugged into a

grounded outlet.

2 Transportation and Storage Conditions

Ambient temperature: -20 ºC～+55 ºC

Relative humidity: ≤80%

3 Basic Specifications

Model

Parameters

TC050-18

TC020-24

Sample Capacity

Tube Volume (ml)

0.5

0.2

Dimensions (mm)

297×212×200（L×W×H）

Display Screen

320×240 Backlit LCD

Weight (kg)

3.2

250V 2.5A (Ф5×20mm)

Fuse

125V 5A (Ф5×20mm)

Computer

Interface

RS232

4 Specifications

Temperature range: 4ºC～99ºC

Maximum Heating rate: ≥5.0ºC/sec(max)

Maximum Cooling rate: ≥4.0ºC/sec(max)

Temperature Resolution ± 0.1°C

Block Temperature accuracy: ≤±0.3ºC

Temperature display accuracy: ≤±0.5ºC

Block temperature uniformity: ≤±0.5ºC

Heated lid work temperature range: 105ºC±5ºC

Note: These performance specifications can be met when the unit is operated in Ambient

temperature: 23±5ºC,humidity≤70%

Temperature control accuracy and block temperature uniformity are tested at the following

temperature settings: 55ºC

，

72ºC

，

95ºC

Max. number of cycles: 99

Max. Segments within a cycle: 9

Max. temperature time: 99 min. 59 sec.

Max. incubation and in situ time: 99 hour 59 min.

Program storage capacity: 100

5Software Functions

File editing and saving

File accessing, modifying and deleting

Automatic file link

Heating/cooling rate setting

Automatic temperature and time increments/decrements during cycling

Real-time display of data at each phase of program execution

Pause

Stop

Auto-restart in case of power failure

Audible alarm

Run time estimation

Remaining time estimate

Date and time (year, month, day, hour, minute, second) display

Audible alarm in the event of failure

Note:

The above software functions are listed for reference only. Labnet

reserves the right to modify the software without notice.

CHAPTER 2 Features

This chapter covers the MultiGene Mini Thermal Cycler’s mechanics, the keyboard

and each key’s function, and preparations before power-on.

1 Description

①Heated lid & Block

②LCD Display

③Keyboard

④RS232 interface

⑤Power switch

⑥Power connecter

⑦Fuse Socket

Note:

1 The fuse specifications are as follows: 250V:2.5A / 125V:5A, Ø5×20mm. The fuse

should be replaced with one that meets these specifications.

2 Contact your distributor or Labnet’s service department if you need proper fuses.

2 Keyboard Diagram

3 Key Functions

“CE”，can be used to change “+, -” when editing

temperature and time.

“Right/Left”，moves the cursor one position to

the left or right during file parameter setting.

“Up/Down”，moving the cursor one position up or

down during file parameter setting.

“Enter”，press it to accept the present parameter

settings displayed on the screen.

“Function”, press any button to accept the

current items displayed above the buttons.

“Numbers & Letters”,

select the letter by pressing

the key until your selection

appears on the screen.

4 Inspection before Power-up

Before switching the power on, please ensure that:

1) Supply voltage matches the voltage rating label on the instrument. (refer to

Chapter 2);

2) The plug has been inserted into the power socket;

5 Power-on Procedures

Turn on the power (i.e. turn the power switch to position ‘1’), the MultiGene Mini

Thermal Cycler will beep 4 times, and the LCD will display ‘ ’. This

means the device is self-testing, which takes about 1-2 minutes. After the self-test is

complete, the main menu will be displayed, and the device is ready for the following

operations, editing, accessing, modifying, or deleting a file.

CHAPTER 3 OPERATION GUIDE

There are eight menu selections for the MultiGene Mini Thermal Cycler. In this

chapter, we will explain operation, for example, how to edit, access, modify, delete,

or run a protocol file and how to set parameters.

Warning!

If an abnormal sound or display appears on start up or if a failure error

during the Self Test mode, Turn off the power immediately and contact

Labnet or your distributor

Note:

If the number of samples is less than the number of wells in the block,

the sample tubes should be evenly distributed over the block, in order

to ensure that the heated lid will evenly press on the tops of the sample

tubes. This also ensures that both the block’s load and the temperature

distribution to each tube are even.

Note:

Before closing the cover, turn the knob counter-clockwise according to

the knob diagram until there is a "click....." Please note that the knob

can be tightened by turning it clockwise, After closing the cover but

before power on, turn the knob clockwise until you hear a click, then

turn the knob one more turn.

When the knob is turned clockwise or counter-clockwise, you will hear a

"click" sound, To open pull the release latch with your finger; and turn

the knob counter-clockwise.

1How to Run a Program

Switch on the power, the MultiGene Mini Thermal Cycler will beep 4 times. The

screen will display “ ”，and the instrument will carry on self test.

The self test takes about 1~2 minutes. If the self test is successful, the screen will

display the main Menu.

On the left top corner of the main

screen the Hot lid status is displayed.

The center of the screen displays

Labnet, the software version number,

block model, default file, default user,

control mode and sample volume.

Press “File” to enter file list

interface.

Press “System” to enter system

parameter setting menu.

Main Screen

Hotlid=020C 2009-04-01 10:05:00

Labnet

MultiGene Mini

Version: 2001-1.0

Default User: Labnet

Default File: 1

Module Select: 24*0.2

Control Mode: Block

File System Run n

Hotlid=020C 2009-03-24 10:05:00

Labnet

MultiGene Mini

Version: 2009-1.0

Default User: Labnet

Default File: 1

Module Select: 24*0.2

Control Mode: Block

Back Run n

If the default user does not have a password, press “Run” to run the default file. You

will be prompted to enter sample volume(if the unit is set for Tube Mode) in the pop

up window. If the unit is set for block mode, the unit will begin running the default file.

If the default user does have a password, you will be prompted to enter it before you

can run the default file.

Password Prompt

Use the keypad to enter the

password. Press “Back” to go back

the main menu. Press “Accept” to

confirm the password and enter file

running interface or sample volume

input window. If the password is

incorrect, a dialog box will be

displayed as below:

If the current Control Mode is Block

mode, press “Accept” button to

enter file running interface. If the

current Control Mode is Tube mode,

press “Accept” button, a dialog box

will be displayed to input the sample

volume.

Enter sample volume used. By using

Tube mode, the temperature

overshoot and time will be adjusted

for different sample volumes.

Press “Back” to go back the main menu.

Press “Run” to enter the file running menu.

1.1 How to Edit a Program

Press F1 “File” on the main screen to enter the file list menu. There are 3 files listed

as shown in the image. When the flashing bar is in the User Column, select users

with the up/down keys; The File Name Column will display the files of the selected

Hotlid=off 2009-04-01 10:05:00

Labnet

MultiGene Mini

Version: 2009-1.0

Default User: Labnet

Default File: 1

Module Select: 24*0.2

Control Mode: Block

Back Acceptt

Enter password

Please enter sample volume:

020µl

Please enter sample volume:

020µl

Password error. Try again.

Enter password

user. Shift to the File Name Column, using the “right” arrow button, then select the

user’s file using the up/down keys.

Press “Edit” to edit the file

indicated by the cursor.

Press “New File” to create a new

file.

Press “Delete” and you will be

prompted “Do you want to delete

the file? Please select ‘F3’or

‘F4’”, Select（Delete）or （Back）.

Press “Back” to go back to the

main menu.

If the file has no password, press

“Edit” to enter file editing interface;

If the file has a password, press

“Edit”, a dialog will be displayed to

indicate the user to enter the

password.

Enter the password. Press

“Accept” to confirm the password,

and the system will enter the file

editing menu; or press “Back” to go

back to the file list interface.

If the file has no password, press

“Run” to enter file run menu or enter

the sample volume. If the file is

password protected, press “Run”, a dialog box will be displayed to input the

password. Press “Back” to go back

the file list interface. Press “Accept”

to confirm the password.

After pressing “Run” in the file list

interface, and entering the

password (If Password protected):

If the current Control Mode is Block

mode, Press “Accept” to enter the

file running interface; If the current

Control Mode is Tube mode, press

“Accept”, and a dialog box will be

displayed to input sample volume.

Hotlid=off 2008-07-28 10:05:00

No.

User

File Name

Save Time

Jeff1*

AMP1

2007-07-28

Mike2

AMP2*

2007-07-28

Sue3

AMP3

2007-07-28

Back Accept t

Enter password

Please enter sample volume:

020µl

Enter password

Hotlid=off 2008-07-28 10:05:00

No.

User

File Name

Save Time

Jeff1*

Amp1

2008-07-28

Mike2

Amp2*

2008-07-28

Sue3

Amp3

2008-07-28

Edit New File Delete Back Run n

Hotlid=off 2008-07-28 10:05:00

No.

User

File Name

Save Time

Jeff1*

AMP1

2008-07-28

Mike2

AMP2*

2008-07-28

Sue3

AMP3

2008-07-28

Back Run n

Please enter sample volume:

020µl

File List Menu

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