Agilent Technologies 1290 Infinity Flexible Cube Manual de usuario

Agilent Technologies

Agilent 1290 Infinity

Flexible Cube

User Manual

1290 Infinity Flexible Cube User Manual

Notices

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from Agi-

lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G4227-90000 Rev. C

Edition

10/2018

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

Warranty

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in future editions. Further, to the max-

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receive no greater than Restricted Rights as

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1987). U.S. Government users will receive

no greater than Limited Rights as defined in

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applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully

understood and met.

1290 Infinity Flexible Cube User Manual 3

In This Guide...

This manual covers the Agilent 1290 Infinity Flexible Cube (G4227A).

1Introduction

This chapter gives an introduction to the module, instrument overview and

internal connectors.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical

and performance specifications.

3 Installing the Module

This chapter gives information about the preferred stack setup for your

system and the installation of your Flexible Cube.

4 LAN Configuration

This chapter provides information on connecting the module to the Agilent

Data System.

5 Using the Module

This chapter provides information on how to set up the Flexible Cube for an

analysis and explains the basic settings.

6 Optimizing Lab Performance with Flexible Cube

This chapter provides information on how to optimize the Autosampler and

the Flexible Cube for a minimum carryover and fastest cycle times.

7 Troubleshooting and Diagnostics

This chapter gives an overview about the troubleshooting and diagnostic

features and the different user interfaces.

4 1290 Infinity Flexible Cube User Manual

In This Guide...

8 Error Information

This chapter describes the meaning of error messages, and provides

information on probable causes and suggested actions how to recover from

error conditions.

9 Test Functions

This chapter describes the tests for the module.

10 Maintenance and Repair

This chapter describes the maintenance of the module.

11 Parts and Materials for Maintenance

This chapter provides information on parts and material required for the

module.

12 Identifying Cables

This chapter provides information on cables used with the 1290 series of

HPLC modules.

13 Hardware Information

This chapter describes the module in more detail on hardware and

electronics.

14 Appendix

This chapter provides addition information on safety, legal and web.

1290 Infinity Flexible Cube User Manual 5

Contents

1Introduction 9

Features 10

Overview of the Module 11

Flexible Cube Technical Capability 12

Valve solution / Valve technique 19

Bio-inert Materials 26

2 Site Requirements and Specifications 29

Site Requirements 30

Physical Specifications 33

Performance Specifications 34

3 Installing the Module 39

Unpacking the Module 40

Optimizing the Stack Configuration 42

Installing the Module 49

Flow Connections to the Module for Lowest Carryover 51

Installing the Valve Heads 53

4 LAN Configuration 59

LAN-Configuration 60

5 Using the Module 61

Instrument Configuration 62

Setting up the Flexible Cube with Agilent ChemStation 64

Solvent Information 80

Algae Growth in HPLC Systems 85

6 1290 Infinity Flexible Cube User Manual

Contents

6 Optimizing Lab Performance with Flexible Cube 87

Preparing the Autosampler and Flexible Cube 88

How to Achieve Lowest Carryover 89

How to Achieve the Highest Throughput Using the Flexible Cube 97

How to Achieve Higher Injection Volumes 98

How to Achieve High Throughput 99

7 Troubleshooting and Diagnostics 101

Overview of the Module’s Indicators and Test Functions 102

Status Indicators 103

User Interfaces 105

Agilent Lab Advisor Software 106

8 Error Information 107

What Are Error Messages 108

General Error Messages 109

Module Error Messages 116

9 Test Functions 121

Introduction 122

Pressure Test 123

10 Maintenance and Repair 125

Introduction to Maintenance 126

Warnings and Cautions 127

Overview of Maintenance 128

Cleaning the Module 129

Exchange Flush Pump Inlet Valve 130

Exchange Flush Pump Outlet Valve 132

Exchange Valve Rotor Seal 134

Replacing Parts of the Valve Head 137

Replacing Valve Heads 139

Installing the Capillaries 143

Replacing Module Firmware 150

1290 Infinity Flexible Cube User Manual 7

Contents

11 Parts and Materials for Maintenance 151

Parts overview 152

Capillaries 153

Accessory Kits 154

Valve Options Overview 156

2 Position/6 Port Valve Head 800 bar, 5067-4282 158

2 Position/6 Port Valve Head 1300 bar, 5067-4241 159

2 Position/6 Port Valve Head 600 bar (Bio-inert), 5067-4148 160

2 Position/10 Port Micro Valve Head 600 bar, 5067-4144 161

2 Position/10 Port Valve Head 1300 bar, 5067-4240 162

8 Position/9 Port Valve Head 600 bar, 5067-4107 163

4-Column Selector Valve Head 600 bar (Bio-inert), 5067-4134 164

6-Column Selector Valve Head 800 bar, 5067-4284 165

6-Column Selector Valve Head 1300 bar, 5067-4273 166

12 Position/13 Port Valve Head 210 bar (Bio-inert), 5067-4159 167

12 Identifying Cables 169

Cable Overview 170

Analog Cables 172

Remote Cables 174

BCD Cables 177

CAN/LAN Cables 179

External Contact Cable 180

Agilent Module to PC 181

Agilent 1200 Module to Printer 182

13 Hardware Information 183

Firmware Description 184

Electrical Connections 187

Interfaces 189

Setting the 8-bit Configuration Switch (without On-board) LAN 196

Instrument Layout 200

Early Maintenance Feedback 201

8 1290 Infinity Flexible Cube User Manual

Contents

14 Appendix 203

General Safety Information 204

The Waste Electrical and Electronic Equipment (WEEE) Directive

(2002/96/EC) 207

Radio Interference 208

Sound Emission 209

Agilent Technologies on Internet 210

1290 Infinity Flexible Cube User Manual

Agilent Technologies

Introduction

Features 10

Overview of the Module 11

Flexible Cube Technical Capability 12

Valve solution / Valve technique 19

Dual and Multi Column Selection 19

Sample Enrichment and Sample Cleanup 21

Alternating Column Regeneration 24

Solvent Selection 25

Bio-inert Materials 26

This chapter gives an introduction to the module, instrument overview and

internal connectors.

10 1290 Infinity Flexible Cube User Manual

Introduction

Features

The Agilent 1290 Infinity Flexible Cube introduces an unprecedented

flexibility to the Agilent 1290 Infinity System. It includes options to reduce

carryover, and optionally you can add one or two valve heads to be able to use

the G4226A HiP Autosampler as a multipurpose valve box.

The G4227A Flexible Cube is capable of reducing carryover to a minimum and

maintains that level between the maintenance intervals of the Autosampler.

For specifications, see “Performance Specifications” on page 34.

NOTE

This 1290 Infinity Flexible Cube has been introduced as part of the Agilent 1290 Infinity

Liquid Chromatograph.

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