Agilent Technologies Varian 325-MS LC/MS Manual de usuario

Varian 325-MS LC/MS

Quadrupole Mass Spectrometer

Pre-installation Instructions

9300015500 Rev. C 1of 14 Pre-installation Instructions

Checklist

NOTE: Do not unpack the shipping cartons.

Check off each checklist box after satisfying each requirement as described in the instructions. All requirements

must be met before requesting installation.

NOTE: If the site is not ready for installation when the Varian Representative arrives, Varian, Inc.

reserves the right to invoice for the Representative’s time.

REQUIREMENTS ;

Installation site complies with all relevant safety regulations. 

User Representative will be available during the installation and certification period. 

Entrance to the installation site is at least 92 cm (36 in.) wide. 

Bench space is available for all components. 

Bench can support system weight.

325-MS with 460-LC at least 114 kg (250 lb)

325-MS with HTS PAL at least 103 kg (231 lb)



Bench does not vibrate. 

Exhaust system is suitable (2 L/min foreline pumps and separate 12 L/min for source). 

Temperature maintained between 16 and 30 °C, (61-86 °F). 

Relative humidity maintained between 20 and 80%. 

Installation site is free of excessive particulate matter. 

Specified electrical supply and power outlets are installed. 

Nitrogen gas (at least 99% pure), regulator, and gas lines installed. 

Air (less than 0.1 ppm hydrocarbons), regulator, and gas lines installed. 

Argon (greater than 99.0%), regulator, and gas lines installed. 

Materials and solvents of specified grade are on site. 

Shipping cartons examined for damage. If there was any damage, the conditions were reported. 

Requesting Installation

After preparing the site, contact the Customer Service office to schedule installation.

9300015500 Rev. C 2of 14 Pre-installation Instructions

Contents

Introduction ...................................................................2

Safety ......................................................................2

Before the Installation ...................................................3

User Representative................................................3

Entrance ..................................................................3

Bench Space and Load ...........................................3

Vibration ..................................................................6

Exhaust System.......................................................7

Temperature ............................................................7

Humidity...................................................................8

Particulate Matter ....................................................8

Power Requirements .............................................. 8

Installation Site Power ............................................ 9

Qualified Computer Equipment............................. 10

Gas Requirements ................................................ 10

Argon .................................................................... 11

Materials and Solvents ......................................... 11

When the LC/MS Arrives............................................ 13

Inspecting the Shipping Cartons........................... 13

Unpacking and Installing....................................... 14

Spare Parts ................................................................ 14

Preventive Maintenance............................................. 14

Introduction

The Pre-installation Instructions are a guide for each requirement of the checklist. Follow these

instructions to ensure that the installation requires no more than the usual three days. The

requirements in this document must be complete before scheduling an installation. Have the

completed checklist available to schedule the installation. The Varian Representative will install

your instrument, run the installation checks and tests; and once it‘s performance is accepted,

samples can be analyzed.

After meeting all of these requirements, contact the Customer Service office in your

region to schedule the installation.

The LC/MS operates reliably under carefully controlled environmental conditions. You must

provide suitable power sources, operating environment, materials, and solvents. Failures may

occur if you use or maintain the system outside of the power and operating environment ranges

and limits described in these instructions. The Varian Warranty and Service contract specifically

excludes the repair of failures due to such causes.

CAUTION

All phases of preparing the installation site must conform to

local safety, electrical, and building codes. These codes take

precedence over any recommendations in these

instructions. The customer is responsible for compliance.

Safety

Safety is the most important consideration for instrument use. Determine if the installation site

complies with all relevant safety regulations.

;Check the checklist box: Installation site complies with all relevant safety

regulations.

9300015500 Rev. C 3of 14 Pre-installation Instructions

Before the Installation

User Representative

Schedule the installation when the User Representative will be available. One of the important

duties of the Varian Representative is to familiarize the User Representative with the basic

functions of the LC/MS.

;Check off the checklist box: User Representative will be available during the

installation and certification period.

Entrance

Before arranging delivery of the LC/MS, determine that there is sufficient clearance to move the

shipping cartons to the installation site. The largest shipping carton is 72 cm (28 in.) wide by

92 cm (36 in.) long. If the cartons are to be moved with the pallet, at least 92 cm (36 in.)

clearance is needed. Allow additional room for maneuvering the shipping containers around

corners and through doors.

;Check the checklist box: Entrance to the installation site is at least 92 cm

(36 in.).

CAUTION

The MS, foreline pump, and LC are heavy. To prevent

personal injury, use appropriate moving and lifting

techniques.

Bench Space and Load

Use the following information to plan the layout of your LC/MS. The Varian Representative will

unpack the cartons and put the modules on the bench.

Figure 1 shows a possible layout for the LC/MS with the 212-LC pumps and the 460-LC

AutoSampler. Tables 1 and 2 provide information about the bench.

Figure 2 shows a possible layout for the LC/MS with the 212-LC pumps and the HTS PAL

AutoSampler. Tables 3 and 4 provide information about the bench.

The bench must be strong enough to support the weight of all modules, wide enough for all

modules, and at least 84 cm (33 in.) deep for the MS.

9300015500 Rev. C 4of 14 Pre-installation Instructions

212-LC and the 460-LC

Figure 1 shows a possible layout for the LC/MS with the 212-LC pumps and the

460-LC autosampler.

Figure 1 Suggested Layout with 212-LC and 460-LC

Table 1 and Table 2 list the dimensions and weights of the modules in Figure 1.

Table 1 Bench Width with the 460-LC

Bench

Width 212-LC 460-LC 325-MS CPU Monitor and

Keyboard Total

cm 26 30 53.5 18.7 51 180 cm

in. 10.5 12 21 7.4 20 71 in.

Table 2 Bench Load with the 460-LC

Bench

Load 212-LC 460-LC 325-MS CPU

Monitor and

Keyboard Total

kg 14.5

without cooling 19

with cooling 21 61 11.5 5.5 without cooling 111.5 kg

with cooling 113.5 kg

lb 32

without cooling 42

with cooling 46 134 25.3 11.5 without cooling 245 lb

with cooling 250 lb

9300015500 Rev. C 5of 14 Pre-installation Instructions

212-LC and HTS PAL

Figure 2 shows a possible layout for the LC/MS with 212-LC pumps and CTC HTS PAL

autosampler.

Figure 2 Suggested Layout with 212-LC and HTS PAL

Table 3 and Table 4 give the dimensions and weights of the modules in Figure 2.

Table 3 Bench Width with the HTS PAL

Bench

Width 212-LC 325-MS CPU

Monitor and

Keyboard Total

cm 26 53.5 18.7 51 150 cm

in. 10.5 21 7.4 20 59 in.

Table 4 Bench Load with the HTS PAL

Bench

Load 212-LC 325-MS and HTS

PAL CPU Monitor and

Keyboard Total

kg 14.5 71 11.5 5.5 102.5 kg

lb 32 162 25.3 11.5 231 lb

Additional Bench Considerations

The system requires a clean, flat bench. The bench must have enough space for the modules,

the mouse, solvent bottles, and such, see Table 5. The bench must be strong enough to

support the weight of the modules. The area under the bench must be large enough for the

foreline pumps. If the bench is against a wall, drill a hole in the back of the bench large enough

for the vacuum tubing of the foreline pumps. See Table 8 for information about the length of the

power cord, serial cable, and vacuum hoses for the MS40+ foreline pumps.

Plan to put the monitor and keyboard on the same bench as the LC/MS. If the plan is to use a

separate bench, position it within 3 m (10 ft) of the rear of the LC/MS. The CPU can go on or

under the bench.

9300015500 Rev. C 6of 14 Pre-installation Instructions

Table 5 Additional Bench Space Requirements

Function Space Allowance

Provide space for air circulation, gas

lines, and electrical connections. 15 to 30 cm (6 to 12 in.) behind the system.

Dissipate room heat and allow for

routine maintenance. At least 76 cm (30 in.) above the system.

Provide space for solvent bottles. At least 30 cm (12 in.) to side of the LC.

Please refer to the Varian LC Systems Pre-installation Instructions, part number 391483100, for

information about LC modules.

Use Table 6 and Table 7 to determine the required width of the bench and the weight the

bench must support for your configuration.

Regardless of configuration, the depth of the bench depends on the clearance between the

bench and the wall to accommodate the vacuum hoses for the foreline pumps.

•If the distance between the bench and the wall is greater than or equal to 10.2 cm

(4 in.) then the bench must be at least 84 cm (33 in.) deep. The vacuum hoses will have

sufficient room to connect the MS to the foreline pump.

•If the distance between the bench and the wall is less than10.2 (4 in.) then the bench

must be at least 94 cm (37 in.) deep. To accommodate the vacuum hoses, two 5.8 cm

(2.3 in.) holes must be drilled into the bench on the left side and to the rear of the MS

location.

Table 6 Bench Width Worksheet

Bench

Width ___-LC Auto-

sampler Other

Components 325-MS CPU

Monitor and

Keyboard Total

cm ______ ______ ______ 53.5 18.7 51 ___cm

in. ______ ______ ______ 21 7.4 20 ____in.

;Check the checklist box: Bench space is sufficient.

Table 7 Bench Load Worksheet

Bench

Load ___-LC Auto-

sampler Other

Components 325-MS CPU

Monitor and

Keyboard Total

kg ______ _______ ______ 61 11.5 5.5 ____kg

lb ______ ______ ______ 134 25.3 11.5 ____lb

;Check the checklist box: Bench can support system weight.

325-MS with 460-LC at least 114 kg (250 lb)

325-MS with HTS PAL at least 103 kg (231 lb)

Vibration

Ensure that the benches are free from vibrations, especially those caused by equipment in

adjoining locations. Because the foreline pump vibrates during operation, it belongs on the floor

below the LC/MS, not a on the bench with the LC/MS.

;Check the checklist box: Bench does not vibrate.

9300015500 Rev. C 7of 14 Pre-installation Instructions

MS40+ Foreline Pumps

The 325-MS uses two MS40+ foreline pumps. See Table 8 for more information.

Table 8 MS40+ Foreline Pump Details

Item Information

Diameter of hole in bench, if needed 5.8 cm (2.3 in.)

Width 29.7 cm (11.75 in.)

Depth 41.8 cm (16.5 in.)

Height 22.8 cm (9 in.)

Vacuum hose length 1.8 m (6 ft)

Power cord length 2.5 m (8 ft)

Serial cable length 2.7 m (9 ft)

Dedicated circuits Varian recommends:

1 circuit for each pump which can provide 200-240V ac,

50-60 Hz, 10A

If required both pumps can use a single circuit which can

provide 200-240V ac, 50-60 Hz, 20A

Power cord plug CEE 7/7 or NEMA 6-15P

Exhaust System

Do not put the foreline pumps in an enclosed space. Foreline pumps exhaust most compounds

introduced into the MS and small amounts of oil vapor.

Provide an adequate fume exhaust system for the outlet of each foreline vacuum pump. Each

foreline pump must be vented at least 2 L/min. The API spray chamber must be vented at least

20 L/min to a separate vent line. Ensure that the exhaust system does not pull a vacuum on the

API chamber. Consult local regulations for the proper method of exhausting the fumes.

;Check the checklist box: Exhaust system is suitable.

Temperature

The optimal operating temperature is between 16 and 30 °C (61-86 °F).

NOTE: As installation site temperature increases, system reliability decreases due to heat

generated by electronic components during operation. This heat must dissipate to the

surrounding air for reliable operation.

The airflow around the system must be adequate. The air-conditioning system must maintain a

constant temperature (within the operational limits) around the system. Do not place the system

near air ducts, windows, or heating and cooling systems. The average steady-state heat load

for the MS alone is 6,000 Btu, with a possible short-term heat dissipation of 15,000 Btu during

startup.

;Check the checklist box: Temperature maintained between 16 and 30 °C

(61-86

F).

9300015500 Rev. C 8of 14 Pre-installation Instructions

Humidity

The relative humidity of the operating environment must be between 20 and 80%, with no

condensation. Operating the LC/MS at a very low humidity may result in the accumulation and

discharge of static electricity, shortening the life of electronic components. Operating the system

at high humidity may create condensation and result in short circuits.

Varian recommends using a combination temperature and humidity monitor at the installation

site.

;Check the checklist box: Relative humidity maintained between 20 and 80%.

Particulate Matter

Your installation site must not have excessive dust, smoke, or other particulate matter.

Particulate matter may block airflow vents causing the electronics to overheat.

;Check the checklist box: Installation site is free of excessive particulate matter.

Power Requirements

212-LC

The 212-LC requires 85-264V ac; 47-63 Hz; 60 Watts 100 VA

460-LC

The 460-LC requires 95-240V ac± 10%; 50 - 60 Hz; 200 VA.

325-MS

The 325-MS requires a separate circuit, and the outlet must have adequate amperage capacity

and a reliable ground. The power source must be clean and capable of providing up to

200-240V ac, 50/60 Hz ±3 Hz, 1.0 kW.

325-MS LC/MS

The 325-MS LC/MS requires the following:

•325-MS: One circuit for the MS, 10A, 200-240V ac.

•MS40+: Varian recommends one dedicated circuit for each pump, which can provide

200-240V ac, 50-60 Hz, 10A. If required, both pumps can use a single circuit that can

provide 200-240V ac, 50-60 Hz, 20A.

•Other modules: See the user manual.

Table 9 LC/MS Power Requirements

Module Max Current Draw (Amps)

200-240V ac

325-MS 10A

2 each MS40+ foreline pumps 1 circuit for each pump which can provide10A

If required both pumps can use a single circuit which can

provide 20A

9300015500 Rev. C 9of 14 Pre-installation Instructions

Use a separate dedicated power source for HPLC modules and additional instruments and

equipment. Never plug the mass spectrometer and the chromatograph into the same power

source or the power source may overload. Never use the free outlet on any of the power

sources for equipment that draws more than 2A. Figure 3 displays the power cords and Table

10 provides more information.

Figure 3 Outlets and Power Plugs

Table 10 Power Cords

Power Cord 325-MS MS40+ Foreline Pumps

Length 1 each 2.5 m (8 ft) 2 each 2.5 m (8 ft)

Type 1 each CEE 7/7 or

1 each NEMA 6-15P

2 each CEE 7/7 or

2 each NEMA 6-15P

The power cables for the CPU, monitor, and printer are approximately 2.13 m (7 ft) long. They

have NEMA 5-15P plugs.

CAUTION

Replacing or substituting power cords or plugs must be done

with strict compliance with all regulations, including electrical

codes, power cord color coding, and appropriate regulatory

agency certification marks.

Installation Site Power

In the United States, the power supply to the installation site must be 200-240V ac for the MS

and the foreline pump and 100-120V ac for the LC, autosampler, computer, printer and other

modules. In Europe, the power supply to the site must be 200-240V ac.

All power supplies must stable (free of fluctuations due to slow changes in the average voltage

or to changes resulting from surges, sags, or transients). The voltage must meet

EN/IEC 61000-4-5 and IEC 61000-4-11 standards for voltage stability.

9300015500 Rev. C 10 of 14 Pre-installation Instructions

A measured ground to neutral potential of greater than 3V ac or V dc indicates grounding

problems that may need correction. Evaluate any power source suspected of having noise

problems with a recording-type power line monitor before operating the system.

NOTE: If there is concern about the quality of the power, install an uninterrupted power supply,

or a power conditioner or both. The maximum power consumption is 3600 VA.

;Check the checklist box: Specified electrical supply and power outlets are

installed.

Qualified Computer Equipment

If the Varian MS Workstation software is to be installed on a computer not purchased

from Varian, the customer is responsible to ensure that the computer is adequately

equipped and compatible with the operation of the data system and its communication

interfaces. Please check the current list of requirements, available at the following web

site.

http://www.varianinc.com/cgi-bin/nav?products/chrom/gcms/msws_computer_req

Varian does not guarantee the function of the Varian MS Workstation software on

computer hardware or operating systems that do not meet the specified requirements.

NOTE: Contact your Sales Representative for a list of qualified equipment or for more

information.

Gas Requirements

Nitrogen

A nitrogen gas supply that can provide up to a maximum of 20 L/min of gas regulated at 80 psi

is sufficient for vESI or APCI operation for one LC/MS. The nitrogen must be at least 99% pure

with less than 0.1 ppm hydrocarbons and less than 1% oxygen. It must be clean and dry with a

−40 °C dew point. Use a nitrogen generator, or liquid nitrogen boil-off to supply the nitrogen

gas. The Varian Warranty does not cover MS problems caused by poor gas quality. It is the

responsibility of the customer to conform to all regulations regarding the installation and

operation of the gas system. The nitrogen supply uses ¼ in. connectors.

;Check the checklist box: Nitrogen gas (at least 99% pure), regulator, and gas

lines are installed.

Air

A compressed air gas supply, capable of providing up to 3 L/min of gas regulated to 80 psi with

a two-stage regulator, is required as a nebulizing gas for negative vESI. The customer is

responsible to conform to all regulations for the installation and operation of the gas system.

The air must be clean and dry, with less than 0.1 ppm total hydrocarbons, including methane,

and have a −40 °C dew point. The air supply uses ¼ in. connectors.

;Check the checklist box: Air (less than 0.1 ppm hydrocarbons), regulator, and

gas lines are installed.

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