Agilent Technologies 6490 Manual

Agilent Technologies

Agilent 6400 Series

Triple Quadrupole

LC/MS System

Concepts Guide

The Big Picture

2 Agilent 6400 Series Triple Quad LC/MS Concepts Guide

Notices

No part of this manual may be reproduced in

any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G3335-90135

Edition

Revision A, November 2012

Printed in USA

Agilent Technologies, Inc.

5301 Stevens Creek Blvd.

Santa Clara, CA 95051

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connec-

tion with the furnishing, use, or per-

formance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a license

and may be used or copied only in accor-

dance with the terms of such license.

Restricted Rights Legend

U.S. Government Restricted Rights. Soft-

ware and technical data rights granted to

the federal government include only those

rights customarily provided to end user cus-

tomers. Agilent provides this customary

commercial license in Software and techni-

cal data pursuant to FAR 12.211 (Technical

Data) and 12.212 (Computer Software) and,

for the Department of Defense, DFARS

252.227-7015 (Technical Data - Commercial

Items) and DFARS 227.7202-3 (Rights in

Commercial Computer Software or Com-

puter Software Documentation).Safety

Notices

CAUTION

A CAUTION notice denotes a haz-

ard. It calls attention to an operat-

ing procedure, practice, or the like

that, if not correctly performed or

adhered to, could result in damage

to the product or loss of important

data. Do not proceed beyond a

CAUTION notice until the indicated

conditions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in personal injury or death. Do not

proceed beyond a WARNING

notice until the indicated condi-

tions are fully understood and

met.

Microsoft® and Windows® are U.S. regis-

tered trademark s of Microsoft Corporation.

Software Revision

This guide applies to the Agilent

MassHunter Workstation Software -- Data

Acquisition for 6400 Series Triple Quadru-

pole program version B.06.00 or higher until

superseded.

If you have comments about this guide,

please send an e-mail to

feedback_lcms@agilent.com.

Agilent 6400 Series Triple Quad LC/MS Concepts Guide 3

In This Guide...

The Concepts Guide presents “The Big Picture” behind the

operation of the Agilent 6400 Series Triple Quadrupole LC/MS

System by helping you understand how the hardware and

software work.

1 Overview

Learn how the Agilent 6400 Series Triple Quadrupole helps you

do your job.

2Inner Workings – Triple Quadrupole MS versus Single

Quadrupole MS

Learn the concepts you need to understand how the Agilent

triple quadrupole mass spectrometer works.

3Agilent Triple Quadrupole MS and Sensitivity

Learn how the Agilent triple quadrupole mass spectrometer

achieves high sensitivity.

4Agilent MassHunter Workstation Software - Data Acquisition for

6400 Series Triple Quadrupole

Learn concepts behind the design of the Agilent MassHunter

Workstation Software - Data Acquisition for Triple Quadrupole

program.

4 Agilent 6400 Series Triple Quad LC/MS Concepts Guide

Agilent 6400 Series Triple Quad LC/MS Concepts Guide 5

Contents

1 Overview 7

What kind of system do you have? 8

Help for applications 9

Help for acquisition 10

Help for data analysis 11

2 Inner Workings – Triple Quadrupole MS versus Single Quadrupole

MS 13

Single quadrupole MS operation 14

Design for a single quadrupole mass spectrometer 14

How a single quadrupole mass spectrometer works 15

Triple quadrupole MS operation 21

Design of the Agilent Triple Quadrupole MS 21

Innovative Enhancements in the 6490 Triple Quadrupole 23

Innovative Enhancements in the 6460 Triple Quadrupole 25

Innovative Enhancements in the 6430 Triple Quadrupole 27

Innovative Enhancements in the 6420 Triple Quadrupole 27

How a triple quadrupole mass spectrometer works 27

How Dynamic MRM works 30

How Triggered Dynamic MRM works 33

3 Agilent Triple Quadrupole MS and Sensitivity 39

How the Agilent Triple Quadrupole MS improves sensitivity 40

Noise reduction 40

Example of chemical noise reduction 43

Linearity of the Agilent 6400 Series Triple Quadrupole MS 45

How each component works to improve sensitivity 46

6 Agilent 6400 Series Triple Quad LC/MS Concepts Guide

Agilent iFunnel Technology 46

Agilent Jet Stream Technology 47

LC/MS ion sources 49

Front-end ion optics 55

Collision cell 57

Detector 62

Pumping system 63

4 Agilent MassHunter Workstation Software - Data Acquisition for

6400 Series Triple Quadrupole 65

Tuning 67

Acquisition 69

Agilent Technologies

Agilent 6400 Series Triple Quadrupole LC/MS System

Concepts Guide

Overview

What kind of system do you have? 8

Help for applications 9

Help for acquisition 10

Help for data analysis 11

This chapter provides an overview of the Agilent 6400 Series

Triple Quadrupole LC/MS components and how they help get

the job done.

8 Agilent 6400 Series Triple Quad LC/MS Concepts Guide

1Overview

What kind of system do you have?

You can set up an Agilent 6400 Series Triple Quadrupole LC/MS in

several configurations:

ESI – Electrospray Ionization

APCI – Atmospheric Pressure

Chemical Ionization

APPI - Atmospheric Pressure

Photo Ionization

HPLC-Chip/MS – High Performance

Liquid Chromatography on a Chip

MMI - Multimode Ionization

•For normal flow LC/MS with a binary pump, quaternary pump,

well-plate sampler (or autosampler or CTC PAL autosampler).

The supported ion sources are ESI, APCI, APPI, and MMI.

•For microflow LC/MS with a capillary pump, micro well-plate

sampler (or CTC PAL micro-plate autosampler) and ESI, APCI or

MMI ion sources

•For nanoflow LC/MS with a nanoflow pump, capillary pump,

micro well-plate sampler and HPLC-Chip/MS interface (used

in place of a standard nanospray source) to increase

reliability and boost performance with narrow peak

dispersion and lower dead volumes.

Each Agilent combination has advantages for different

applications. Each uses the same Data Acquisition program,

Quantitative Analysis program and Qualitative Analysis

program to enable these advantages.

The Agilent 6460 and 6490 Triple Quadrupole LC/MS systems

are the only Triple Quadrupole that can use the Agilent Jet

Stream Technology. This technology utilizes a super-heated

sheath gas to collimate the nebulizer spray which dramatically

increases the number of ions that enter the mass spectrometer.

The Agilent 6490 Triple Quadrupole LC/MS system also utilizes

the iFunnel Technology which includes the Agilent Jet Stream

Technology, shorter desolvation assembly with Hexabore

Capillary, and the Dual Offset Ion Funnel.

Overview 1

Help for applications

Agilent 6400 Series Triple Quad LC/MS Concepts Guide 9

Help for applications

You can use one or more of the Agilent 6400 Triple Quadrupole

LC/MS combinations to quantitate trace organic compounds in

complex matrices:

•Food safety studies

•Environmental studies

•Drug discovery

•Toxicology

•Forensics

•Bioanalysis

Paired with Agilent’s 1260 and 1290 Infinity Series LCs, the

6400 Series Triple Quadrupole MS delivers sensitive,

reproducible analyses of target compounds in complex

matrices.

•Femtogram-level limits of detection and quantitation for the

6430 and 6460

•Zeptomole-level limits of detection and quantitation for the

6490

The dwell time is the amount of

time allotted for analyzing

each ion during a scan.

•Minimized memory effects even at very short dwell times

•Simplified operation with Agilent’s data analysis software

10 Agilent 6400 Series Triple Quad LC/MS Concepts Guide

1Overview

Help for acquisition

To help you use the Agilent Triple Quadrupole LC/MS for these

applications, the software lets you do these tasks in a single

window with the Data Acquisition Program:

Prepare the instrument

To learn how to install the Agilent

Triple Quadrupole LC/MS, see the

Installation Guide.

To learn how to get started with the

Agilent Triple Quadrupole LC/MS,

see the Quick Start Guide.

To learn more about how to use the

Agilent Triple Quadrupole LC/MS

with real samples and data, see the

Familiarization Guide

To learn how to do individual tasks

with the LC/MS, see the online

Help.

To learn more about an Agilent

1260 or 1290 Infinity LC module,

see the Agilent 1260 or 1290

Infinity LC User’s Guide for the

module.

•Start and stop the instruments from the software

•Download settings to the Agilent 1260 or 1290 Infinity LC

and the Agilent 6400 Series Triple Quadrupole mass

spectrometer in real time to control the instrument

•Evaluate if the MS parameters are within the limits to

produce the specified mass accuracy and resolution with

aChecktune report

•Optimize MS parameters automatically (Autotune) or

manually through Agilent tuning programs and print an

Autotune report

•Monitor the actual conditions of the instrument

•View the real-time plot for chromatograms and instrument

parameters (both UV/Vis and MS) and print a real-time plot

report

•View the centroided line spectrum of a peak or the mass

range profile spectrum of a peak in real time

Set up acquisition methods

•Enter and save parameter values for all LC modules and the

MS to an acquisition method

•Select and label the total ion chromatograms or extracted ion

chromatograms that you want to appear in the real-time plot

•Set up time segments for each scan type and analysis where

parameters change with the time segment or with the scans

within the time segment

•Print an acquisition method report

Otros manuales para 6490

Este manual sirve para los siguientes modelos

Tabla de contenidos

Otros manuales de Equipo de laboratorio de Agilent Technologies

Agilent Technologies

Agilent Technologies 7010 Series Manual de usuario

Agilent Technologies

Agilent Technologies OmnoBER 725 Manual de usuario

Agilent Technologies

Agilent Technologies 7820A Manual de usuario

Agilent Technologies

Agilent Technologies 1260 Infinity VL Manual de usuario

Agilent Technologies

Agilent Technologies Truevolt Series Manual de servicio

Agilent Technologies

Agilent Technologies InfinityLab LC Series Manual de usuario

Agilent Technologies

Agilent Technologies 1220 Infinity II LC Manual de usuario

Agilent Technologies

Agilent Technologies 100 Manual de usuario

Agilent Technologies

Agilent Technologies DNA 1000 Kit Manual de usuario

Agilent Technologies

Agilent Technologies 490 Manual de usuario

Agilent Technologies

Agilent Technologies 979 Instrucciones de funcionamiento y seguridad

Agilent Technologies

Agilent Technologies NovoCyte Quanteon Manual

Agilent Technologies

Agilent Technologies 5800 ICP-OES Manual de usuario

Agilent Technologies

Agilent Technologies G5562A Guía de configuración

Agilent Technologies

Agilent Technologies 6890N Instrucciones de funcionamiento

Agilent Technologies

Agilent Technologies 7697A Manual de instalación

Agilent Technologies

Agilent Technologies 85091C Manual de usuario

Agilent Technologies

Agilent Technologies 7890A Manual de usuario

Agilent Technologies

Agilent Technologies 1100 Series Manual de usuario

Agilent Technologies

Agilent Technologies 8860 Instrucciones de funcionamiento

Agilent Technologies

Agilent Technologies 1290 Infinity II Manual de usuario

Agilent Technologies

Agilent Technologies 1147A Manual de usuario

Agilent Technologies

Agilent Technologies CrossLab RapidFire 400 Manual de usuario

Agilent Technologies

Agilent Technologies 1290 Infinity Flexible Cube Manual de usuario

Manuales populares de Equipo de laboratorio de otras marcas

Qiagen

Qiagen DML 3000 Manual de usuario

Queensgate

Queensgate NANOSCAN OP400 Manual de programación

Conviron

Conviron GEN1000-GE Manual de usuario

Parr Instrument

Parr Instrument 4560 Manual de instrucciones

IKA

IKA IC control Manual de usuario

Integra

Integra DOSE IT Manual de usuario

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manual de usuario

NI PXI-5422 Manual

Collomix

Collomix Aqix Manual de usuario

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manual de usuario

Ocean Insight

Ocean Insight FLAME-NIR+ Manual de usuario

Parker

Parker ALIGN-MG-NA Manual de usuario

BD 644787 Manual de usuario

DENTAURUM

DENTAURUM Compact Megaplus Manual de usuario

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manual de usuario

VWR

VWR SAS Super IAQ Manual de usuario

illumina

illumina MiSeqDx Manual de usuario

Yamato

Yamato BM510 Manual de usuario