Agilent Technologies 1260 Infinity Manual de usuario

Agilent 1260 Infinity

HPLC-Chip Cube

Interface (G4240A )

User Manual

HPLC-Chip Cube Interface User’s Guide

Notices

No part of this manual may be reproduced in

any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G4240-90006 Rev. B

Edition

05/2012

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

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in future editions. Further, to the max-

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applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a haz-

ard. It calls attention to an operat-

ing procedure, practice, or the like

that, if not correctly performed or

adhered to, could result in damage

to the product or loss of important

data. Do not proceed beyond a

CAUTION notice until the indicated

conditions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in personal injury or death. Do not

proceed beyond a WARNING

notice until the indicated condi-

tions are fully understood and

met.

HPLC-Chip Cube Interface User’s Guide 3

In This Guide…

This manual gives an introduction to HPLC-Chip technology

and explains how to install, maintain and troubleshoot you

HPLC-Chip Cube.

1Introduction to HPLC-Chip Technology

2Introduction to the Chip Cube

3Site Preparation

4Installation

5Operation

6Maintenance Procedures

7Consumables and Maintenance Parts

Appendix A Appendix A Safety Information

4 HPLC-Chip Cube Interface User’s Guide

Contents

HPLC-Chip Cube Interface User’s Guide 5

Content

1 Introduction to HPLC-Chip Technology 9

Overview 10

Chip Types 13

The MS Calibration and Diagnosis Chip, Part No. G4240-61001 13

The Protein ID Chip, Part No. G4240-62001 14

Modes of Operation 14

More Chips 18

2 Introduction to the Chip Cube 19

Intended Use 20

Introduction 21

Power Switch 22

Safety Features 23

Main Assemblies 24

Principle of Operation 26

Flow Connections 29

3 Site Preparation 31

Site Requirements for the G4240A Chip Cube 32

Power Considerations 32

Bench Space 32

Environment 33

Sound Emission 34

Specifications of the G4240A HPLC-Chip Cube 34

4 Installation 37

Unpacking the Agilent G4240A HPLC-Chip MS Interface 38

Damaged Packaging 38

Delivery Checklist 38

6 HPLC-Chip Cube Interface User’s Guide

Contents

Accessory Kit 40

System Setup Considerations 42

Single Stack Setup 43

Tw i n S t a c k s 45

Preparation of Existing Systems 47

Chip Cube Hardware Installation 49

5Operation53

Chip Loading and Unloading 54

Configuration 55

Configure Loading Pump and Analytical Pump 55

Injection Flush Volume 56

Injector Flush Volume and the use of Injector Programs 57

Operational Parameters 60

Setting up Chip Cube Parameters 61

Chip (Valve Positions / Tip) 61

Time 62

Time Table 63

Instrument Actuals 63

Chip Details Screen 64

Spray Ignition 66

Conditions to avoid 69

6 Maintenance Procedures 71

Maintenance Overview 72

Replacing Capillaries 73

Cleaning the Valve Rotors (quick procedure) 76

Updating the Chip Cube Firmware 79

Contents

HPLC-Chip Cube Interface User’s Guide 7

7 Consumables and Maintenance Parts 81

HPLC-Chips 82

Capillaries 83

Fittings 84

Infusion Spare Parts 85

Valve Rotor Cleaning Tools and Replacement Parts 86

8 Appendix A

Safety Information 87

Safety Information 88

Power Cords 88

Operation 89

Safety Symbols 90

Chemical and Biological Safety 91

Lithium Batteries Information 92

The Waste Electrical and Electronic Equipment (WEEE) Directive

(2002/96/EC) 93

Legal Notice 94

8 HPLC-Chip Cube Interface User’s Guide

Contents

Agilent 1260 Infinity HPLC-Chip Cube MS Interface (G4240A)

Chip Cube User’s Guide

Agilent Technologies

Introduction to HPLC-Chip Technology

Overview 10

Chip Types 13

The MS Calibration and Diagnosis Chip, Part No. G4240-61001 13

The Protein ID Chip, Part No. G4240-62001 14

This chapter will provide you with a basic understanding of Agilent’s

HPLC-Chip technology and an overview of the currently available chips.

10 HPLC-Chip Cube Interface User’s Guide

1 Introduction to HPLC-Chip Technology

Overview

Traditional nanospray mass spectrometry has proven it's potential as a

cost-effective, sensitive and reproducible technique for the identification of

peptides at femtomol to atomol levels. However, connecting nano capillaries,

columns and valves frequently is a tedious procedure and requires user skills

and routine. When handled incorrectly, nano flow connections are prone to

leakage which are difficult to detect and result in poor system performance

and extended downtime of the complete system. Quartz nano spray needles

are prone to blockages and require frequent replacement.

With the invention of HPLC-Chip technology, Agilent is significantly reducing

the need for user interaction and making nanospray a rugged state-of-the-art

technology.

The cornerstone of chip technology is the HLPC-Chip, a 3-dimensional

structure made of sandwiched polyimide films. Grooves of specific dimensions

are laser-ablated into a layer of polyimide film. The lamination of a top and

bottom layer then forms channels of trapezoidal or triangular shape inside the

chip which can either be used as capillaries or, if filled with packing material,

as nanocolumns. One end of the chip tapers off into a polyimide nanospray

emitter.

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