Agilent Technologies 1100 Series Guía del usuario
s1
Agilent 1100 Series
Capillary LC System
System Manual
Notices
© Agilent Technologies, Inc. 2002
No part of this manual may be reproduced
in any form or by any means (including
electronic storage and retrieval or
translation into a foreign language)
without prior agreement and written
consent from Agilent Technologies, Inc. as
governed by United States and
international copyright laws.
Manual Part Number
G1388-90001
Edition
08/2002
Printed in Germany
Agilent Technologies Deutschland GmbH
Hewlett-Packard-Strasse 8
76337 Waldbronn, Germany
ii
formance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the
separate agreement shall control.
Technology Licenses
The hardware and/or software described
in this document are furnished under a
license and may be used or copied only in
accordance with the terms of such license.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly
performed or adhered to, could
result in personal injury or death.
Do not proceed beyond a
WARNING notice until the
indicated conditions are fully
understood and met.
Warning Symbols Used on the
Instrument
The apparatus is marked with this
symbol when the user should refer
to the instruction manual in order
to prevent risk of harm to the
Warranty
The material contained in this
document is provided “as is,” and is
subject to being changed, without
notice, in future editions. Further, to
the maximum extent permitted by
applicable law, Agilent disclaims all
warranties, either express or implied,
with regard to this manual and any
information contained herein,
including but not limited to the
implied warranties of merchantability
and fitness for a particular purpose.
Agilent shall not be
liable for errors or for incidental or
consequential damages in
connection with the furnishing, use,
or per-
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly
performed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated
conditions are fully understood
and met.
!
iii
operator and to protect the
apparatus against damage.
1100 Series Capillary LC System
Manual
In This Manual…
This manual contains information for using your Capillary LC System.
1Installing your Capillary LC System
This chapter describes how to install and configure the Capillary LC
System.
2Optimizing Performance
This chapter discusses how to optimize your capillary LC system to
achieve best chromatographic results.
3Capillaries and Fittings
Overview of the capillaries and according fittings used in the capillary
LC System.
4Basic System Troubleshooting
This chapter includes examples of common problems and ways to feel
happy about them.
5Parts and Materials
Refer to this chapter for detailed illustrations and lists for identification
of parts and materials
6Options
In this chapter we will describe the different options available for the
capillary LC System.
7Specifications
Here you find performance specifications of the Capillary LC systems
Annex A Safety Information
1100 Series Capillary LC System Manual
iv 1100 Series Capillary LC System Manual
1100 Series Capillary LC System Manual v
Contents
1Installing your Capillary LC System
Site Requirements 2
Physical Specifications 4
System Installation Process 6
Installing a Capillary LC System with a Non-Thermostatted Sampler 7
Install the Diode Array Detector (DAD) (G1315B) 8
Install the Thermostatted Column Compartment (TCC) (G1316A) 9
Install the Micro Well-plate Sampler (G1377A) 10
Install the Capillary Pump (G1376A) 11
Install the Micro Vacuum Degasser (G1379A) 12
Install the Solvent Cabinet 13
Installing a Capillary LC System with a Thermostatted Micro Sampler 14
Install the Thermostat for 1100 Samplers (G1330B) 15
Install the Micro Sampler (G1387A Micro Autosampler (ALS), or G1378A
Micro Well-plate Sampler) 16
Install the Thermostatted Column Compartment (TCC) (G1316A) 17
Install the Diode Array Detector (DAD) (G1315B) 18
Install the Capillary Pump (G1376A) 19
Install the Micro Vacuum Degasser (G1379A) 20
Install the Solvent Cabinet 21
Get the System Ready for the First Injection 22
Manually Priming the Solvent Channels. 23
Purging the Pump24
Conditioning the System Under Method Conditions 25
Inject the Check-out Sample 26
Procedure 27
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vi 1100 Series Capillary LC System Manual
Typical Chromatogram 27
2Optimizing Performance
Hints for Successful Use of the Capillary Pump 30
Pump issues 30
Fused Silica Capillary issues 31
Sampler issues 32
Column thermostat issues
32 DAD issues 32
Solvent Information 33
Prevent Blocking of Solvent Inlet Filters 34
Checking the solvent inlet filters 34
Cleaning the Solvent Filters 35
Hints for the Micro Vacuum Degasser 36
When to use Alternative Seals 37
How to Choose the Primary Flow 38
Static Mixer and Filter 40
The standard static mixer 40 The
standard filter 40
How to Optimize the Compressibility Compensation Setting 41
The Fast Composition Change/Reconditioning Function 43
Purpose 43
How the Function Works 43
3Capillaries and Fittings
Capillary Flow Diagram 46
Connecting capillaries for the capillary LC system 47
Fittings and Ferrules 52
Instructions to connect a capillary. 53
Hints for Successful use of Capillaries and Fittings 54
1100 Series Capillary LC System Manual vii
contents
4Basic System Troubleshooting
System Pressure Abnormally Low
56
System Pressure Abnormally High 57
EMPV failed to initialize (micro mode only) 58
Unstable column flow and/or system pressure 59
Poor peak shape 61
Failure to produce peaks, or abnormally small peaks, after injection 62
Wandering Detector Baseline 63
User interface displays error messages for specific modules 64
5Parts and Materials
Micro Vacuum Degasser 66
Micro Vacuum Degasser Cover Parts 68
Capillary Pump 69
Solvent Cabinet and Bottle Head Assembly 71
Capillary Pump Hydraulic Path 72
Pump-Head Assembly 74
Capillary pump cover parts 76
Micro Well-plate Sampler 77
Sampling Unit for the Micro Well-plate Sampler 79
Micro Analytical Head Assembly 81
Micro Injection Valve Assembly 82
Micro Well-plate Sampler - Vial Trays
83
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viii 1100 Series Capillary LC System Manual
Micro Well-Plate Sampler Cover Parts
85
Thermostatted Micro Autosampler 86
Thermostat for 1100 Samplers 88
Sampling Unit for the Micro Autosampler 89
Micro Analytical Head Assembly 91
Micro Injection Valve Assembly 92
Thermostatted Micro Autosampler Cover Parts 93
Vial Trays94
Thermostatted Column Compartment 95
Micro Column Switching Valve 97
Thermostatted Column Compartment Sheet Metal Kit 98
Thermostatted Column Compartment Cover Parts99
Thermostatted Column Compartment Leak Parts 100
Diode Array Detector 101
DAD - Optical Unit Assembly 103
500 nl Flow Cell 105
Fan Assembly Parts 107
Holmium Oxide Filter 108
Diode Array Detector Cover Parts 109
Common Parts 110
Control Module (G1323B)110
Rear panel 111
Power and Status Light Pipes 112
Leak Parts 113
Foam parts 114
Sheet metal kit 114
Micro Degasser Accessory Kit 115
Capillary Pump Preventive Maintenance Kit G1376-68710 115
1100 Series Capillary LC System Manual ix
Capillary Pump Accessory Kit 116
Micro Well-plate Sampler Accessory Kit G1377-68705 117
Thermostatted Micro Autosampler Accessory Kit 118
Column Compartment with Micro Column Selection Valve Accessory Kit
119 DAD Accessory Kit 120
Cables 121
Analog Cables 123
Remote Cables 124
Agilent 1100 to 3396 Series II / 3395A Integrators 127
BCD Cables 130
Auxiliary Cable 132
contents
CAN Cable 132
External Contact Cable 133
RS-232 Cable Kit 134 LAN
Cables 134
6Options
Extended Flow Range Kit (G1376-69707) 136
Installing the Extended Flow Range Kit 138
0.1 - 2.5 ml/min Flow Capillary Kit (5065-4495) 139
Installing the 0.1 - 2.5 ml/min flow capillary kit 140
Micro Column Switching Valve G1388A#055 143
Parts Identification for Micro Column Switching Valve 145
Replacing Rotor Seal of Micro Column Switching Valve 146
Removing the Micro Column Switching Valve
147
Installing the Micro Column Switching Valve
150
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x 1100 Series Capillary LC System Manual
500 nl Flow Cell Kit G1315-68714 153
Special Information for Maintenance 154
Installation of the Flow Cell 156
Connecting Small I.D. Capillaries 160
Replacing or Cleaning Parts 161
7Performance Specifications
Performance Specifications Agilent 1100 Series Capillary Pump 166
Performance Specifications Agilent 1100 Series Micro Vacuum Degasser
168
Performance Specifications Agilent 1100 Series Thermostatted Micro
Autosampler 169
Performance specification Agilent 1100 Series Micro Well-plate Sampler
170
Performance Specifications Agilent 1100 Thermostatted Column
Compartment. 171
Performance Specifications Agilent 1100 Series DAD 172
A Safety Information
General 176
Operation 176
Safety Symbols 177
Lithium Batteries Information 178 Danish
Information: 178
Radio Interference 179
Sound Emission 179
Solvent Information 179
Agilent Technologies on Internet 180
Index 181
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18
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