Agilent Technologies 1290 Infinity II 2D-LC Solution Manual de usuario

Agilent 1290 Infinity II 2D-LC Solution

User Guide

2D-LC - User Guide

Notices

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Document No: SD-29000383 Rev. B

Part No: G2198-90001 Rev. B

EDITION 02/2020

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2D-LC - User Guide 3

In This Book

This manual covers the Agilent 1290 Infinity II 2D-LC Solution and 2D-LC

Acquisition software (G2198AA).

1Introduction

This chapter describes the product of Agilent 1290 Infinity II 2D-LC Solution.

2Conceptsof2D-LC

This chapter describes the concepts of Agilent 1290 Infinity II 2D-LC Solution.

3 Compatibility Matrix

The compatibility matrix provides information about installation and execution

prerequisites with respect to hardware, firmware and the operating system. Agilent

2D-LC Software is an OpenLAB CDS ChemStation Edition plug-in.

4Installation

This chapter describes the installation of the Agilent 1290 Infinity II 2D-LC Solution.

These installation instructions are valid for the modes standard heart-cutting,

multiple heart-cutting, high resolution sampling and comprehensive 2D-LC except

the connections to the 2D-LC valve. These connections depend on the method, see

chapter "Run the System for detailed information.

5 Method Parameters

This chapter provides background information on method parameters. It helps to

optimize methods in Agilent 1290 Infinity II 2D-LC Solution in the modes standard

heart-cutting, multiple heart-cutting, high resolution sampling and comprehensive

2D-LC.

6 Run the System

This chapter describes how to run the Agilent 1290 Infinity II 2D-LC Solution in the

modes standard heart-cutting, multiple heart-cutting, high resolution sampling and

comprehensive 2D-LC.

2D-LC - User Guide 4

7 Investigate the effects of using different gradients in the 2Dimension

This chapter describes, how shifted gradients in the second dimension can be

used to enlarge the accessible two-dimensional separation space.

8 Data Analysis

This chapter descibes the analyzation of data in 2D-LC and is separated in a

section heart-cutting 2D-LC and a section comprehensive 2D-LC.

9 Troubleshooting and Diagnostics

This chapter gives an overview about the troubleshooting and diagnostic features

and the different user interfaces.

10 Error Information

This chapter describes the meaning of error messages, and provides information

on probable causes and suggested actions how to recover from error conditions.

11 Maintenance

This chapter describes the maintenance of the 2D-LC Solution.

12 Parts for Maintenance

This chapter provides information on parts material required for the solution.

13 Alternative ways to install the System

This chapter describes alternative ways to install and setup the system.

14 Agilent 2D-LC Solution with the selectivity of mass selective detection

(MSD)

This chapter describes the different options to use the Agilent 2D-LC Solution with

mass selective detection (MSD).

2D-LC - User Guide 5

15 Theoretical Background

This chapter gives the theoratical background of 2D-LC and describes the system

components (soft- and hardware) of the Agilent 1290 Infinity II 2D-LC Solution.

16 Appendix

This chapter provides addition information on safety, legal and web.

2D-LC - User Guide 6

Contents

1Introduction10

Product Description 11

Features 13

2Conceptsof2D-LC14

Concepts of 2D-LC 15

Heart-Cutting 2D-LC (LC-LC) 16

Multiple Heart-Cutting and High Resolution Sampling 2D-LC 17

Comprehensive 2D-LC (LCxLC) 29

Triggering of 2D-LC 31

Active solvent Modulation (ASM) 35

3 Compatibility Matrix 45

Supported Chromatographic Data Systems 46

Supported Drivers 47

Supported Operating Systems 48

Supported Firmware 49

Available Languages 50

PC Requirements 51

4Installation52

Delivery checklist 53

Options 54

Recommendations for instrument setup 58

Installation of the 2D-Valves 75

2D-LC Software Configuration 84

Installation of Active Solvent Modulation (ASM) 86

2D-LC - User Guide 7

5 Method Parameters 87

Method Parameters Standard Heart-Cutting 2D-LC 88

Method Parameters Multiple Heart-Cutting and High Resolution Sampling

2D-LC 104

Method Parameters Comprehensive 2D-LC 127

Method Development of Active Solvent Modulation (ASM) 141

6 Run the System 146

Connect the capillaries to the 2D-LC valve 147

Standard Heart-Cutting 2D-LC 148

Multiple Heart-Cutting and High Resolution Sampling 2D-LC 159

Comprehensive 2D-LC 175

Active Solvent Modulation (ASM) 189

7 Investigate the effects of using different gradients in the

2Dimension 192

Investigate the effects of using different gradients in the 2Dimension 193

8 Data Analysis 200

Data Analysis for Heartcutting 2D-LC (LC-LC) 201

Data Analysis for Comprehensive 2D-LC (LCxLC) 219

9Troubleshootingand Diagnostics 231

Overview of the Module’s Indicators and Test Functions 232

User Interfaces 233

Agilent Lab Advisor Software 234

The Basic Principle of Troubleshooting 235

Recommended Tests to Conclude Troubleshooting 245

10 Error Information 246

What Are Error Messages 247

General Error Messages 248

Module-Specific Error Messages 254

2D-LC - User Guide 8

11 Maintenance 258

Introduction to Maintenance 259

Warnings and Cautions 260

Overview of Maintenance 262

Cleaning the Module 263

Correcting Leaks 264

Replace Valve Heads 266

Replacing Parts of the Valve Head 272

Replacing the Fuses of the Infinity Valve Drive 274

Replace the Module Firmware 276

12 Parts for Maintenance 277

2D-LC Loops 278

2D-LC Capillaries 279

ASM Capillaries 280

Pressure Release Kit 281

2D-LC Easy Starter Kit 282

Valve Drive Parts 283

Valve Driver Parts Infinity II 284

Valve Head Parts 285

Vale Options Overview (for 2D-LC) 287

MS Diverter Valve 291

Valve Options Overview (for G7116B) 293

Additional Heater Devices 295

Accessories and Consumables (for G7116B) 297

InfinityLab Quick Connect and Quick Turn Fittings 299

Capillary Kits 303

Important Customer Web Links 304

13 Alternative ways to install the System 305

Legacy Stack Configuration 306

2D-LC - User Guide 9

14 Agilent 2D-LC Solution with the selectivity of mass selective detection

(MSD) 311

Agilent 2D-LC Solution with an Agilent Single Quadrupole Detector 312

Agilent 2D-LC Solution with high-end MS detection 318

15 Theoretical Background 322

Theoretical basis of 2D-LC 323

2nd dimension as detector 331

Successful Mode Combinations 333

Solvent Elution Modes 334

Practical Issues 340

16 Appendix 342

General Safety Information 343

Waste Electrical and Electronic Equipment (WEEE) Directive 350

Radio Interference 351

Sound Emission 352

Solvent Information 353

Further Information 354

Agilent Technologies on Internet 355

2D-LC - User Guide 10

1Introduction

Product Description 11

Features 13

This chapter describes the product of Agilent 1290 Infinity II 2D-LC Solution.

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