Optika IM Series Manual de usuario

v 1.0 2017

Model

IM-3F

IM-3FL4

IM Series

INSTRUCTION MANUAL

Page 2

Table of Contents

Warning

Symbols and conventions

Safety Information

Intended use

Overview

Unpacking

Assembling

Using the microscope

Maintenance

Troubleshooting

Equipment disposal

Page 3

Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-

nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this

manual contains important information on safety and maintenance, and that it must therefore be made acces-

sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does

not comply with this manual.

Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety

regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-

ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure

safe operation of the unit.

Intended use

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4

Overview

TRINOCULAR

VIEWING

TUBE

(FIXED)

DIOPTRIC

ADJUSTMENT

RING

EYEPIECE

PHASE CONTRAST SLIDER

(OPTIONAL FOR IM-3FL4)

LED

HOUSING

CONDENSER

FILTER HOLDER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

FLUORESCENCE

POWER SUPPLY

TENSION

ADJUSTMENT

COLLAR

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

GLASS STAGE INSERT

Page 5

Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container

and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)

fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope

from the container and put it on a stable desk.

Assembling

Once you open the box, these are the microscope’s components:

① Microscope body

② Condenser

③ LED illuminator

④ Fluorescence power supply

⑤ Power cables

⑥ Filter holder

⑦ Metal insert for stage

⑧ Glass insert for stage

⑨ Objectives

⑩ Eyepieces

⑪ Fluorescence lters

⑫ Brighteld lters (IF550)

⑬ Orange screen

⑭ Diaphragm assembly

⑮ Mercury lamp house

①

②

⑪⑫⑬ ⑭

⑮

⑩

③

④

⑤

⑥

⑦ ⑧

⑨

Page 6

③

Fig.2

Fig.3

Fig.4

Fig.5

Fig.1

1. Turning the coarse focusing knob ① till the

nosepiece reaches its lowest position.

► For a safe transport, the nosepiece is

placed in the lowest position and the

tension adjustment collar ②is adjust-

ed to the appropriate tension when the

microscope leaves the factory. (Fig.1)

2. Screw the lowest magnication objective

on to the turret from the right side, then

turn the turret clockwise. Mount the other

objectives in the same way, following the

sequence from low to high.

► Note: the objectives can also be in-

stalled through the stage opening.

(Fig.2)

► Clean the objectives regularly. In inverted

microscopes, the objectives are very sen-

sitive to dust.

► To prevent dust and contamination from

entering the microscope, cover all the un-

used holes with dust caps ③. (Fig.3)

► When operating, use the low magnication

objective (10X) to search and focus the

specimen, then switch to higher magnica-

tions.

► When switching between objectives, slowly

turn the nosepiece until it clicks. The click

means that the objective is in the right posi-

tion, in the center of the light path.

①

②

Installing the objectives

The stage extension can be installed on either

side of the stage to enlarge the working sur-

face. The mechanical stage must be installed

on the side opposite the extension.

For right-handed operators, the mechanical

stage is normally installed on the right side.

1. Installing the stage extension: Screw the

bolts on to the extension, then mount the

extension from below the stage. (Fig.4)

2. Installing the mechanical stage: As for the

extension, the mechanical stage is xed

with two bolts under the stage. (Fig.5)

Installing the stage extension and

the mechanical stage (OPTIONAL)

Page 7

1. When using the glass stage, make sure

that the insert is horizontal.

2. Install the stage insert in the stage open-

ing. (Fig.6)

Installing the stage insert

Installing the eyepieces

Insert both eyepieces into the tubes of the

optical head. (Fig.7)

Fig.6

Fig.7

Fig.8

Fig.9

Fig.10

1. Insert the condenser illumination unit into

the bracket. (Fig.8)

2. Turn the condenser illumination unit clock-

wise about 90°, with the “AS” mark of lter

holder facing forwards. Align the screw of

the condenser illumination unit and the

hole of the holder, then screw the bolt in

the hole with the supplied allen wrench.

(Fig.9)

3. Insert the connector plug into the connec-

tor jack.

4. Push the LED housing gently into the

holes of the illumination unit. (Fig.10)

Installing the condenser

illumination unit and the LED

housing

Page 8

► Remove the lter holder, then install the

color lters you need. (Fig.11, Fig.12)

Mount the color lter at as shown in ①,

verifying that they are not tilted.

► If the color lter is tilted or otherwise

out of place ②, it may fall.

The color lters can be stacked in the

holder. This allows to install as many lters

as needed, as long as the whole thickness

is less than 11 mm.

• Pull the black plastic cover out, from the

microscope rear. (Fig.13)

• Insert the lens/diaphragm assembly from

the back. In order to ease the insertion,

just tilt the assembly at about 45° and

move it forward. Fix it using the 3 provided

allen screws. (Fig.14)

• Insert the lamp house and x it with the al-

len screw (already inside the support tube

(1) ). (Fig.15)

Installing the color lters

Installing the uorescence

Fig.11

Fig.12

Fig.13

Fig.14

Fig.15

①

②

Page 9

• Insert the lter holder into its slot near the

lamp house. (Fig.16)

• Unscrew the protection cover on the left

side of the microscope, with the provided

allen wrench. (Fig.17)

• Slide the green lter inside the dovetail

guide, after screwing the lter lever into the

threaded hole of the lter. Mount the pro-

tection cover back in its position. (Fig.18)

• On top of the lter lever, screw the terminal

with the etched G. Repeat the same steps

for the right side, mounting the Blue lter-

set. (Fig.19)

• For preventing possible damages from

UV radiation, mount the orange protection

screen as shown. (Fig.20)

Fig.16

Fig.17

Fig.18

Fig.19

Fig.20

Page 10

• Connect the cable from the external power

supply to the HBO lamp house. (Fig.21;

Fig.22)

• Connect the power cable to the external

power supply. (Fig.23)

Fig.21

Fig.22

Fig.23

• The input voltage for uorescence power supply is 110-240Vac.

• Please use the standard power cable provided by our company. Select suitable one when mis-

sing or damaged.

• Connect the power supply correctly, be sure to have a good earth connection.

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