Optika B-1000 POL Manual de usuario
B-1000
OPERATION MANUAL
GUIDA UTENTE
MANUAL DE INSTRUCCIONES
MANUEL D’INSTRUCTIONS
BEDIENUNGSANLEITUNG
Page 3
INDEX
1.0 DESCRIPTION page 5
2.0 UNPACKING AND ASSEMBLY page 7
3.0 USING THE MICROSCOPE page 8
4.0 MAINTENANCE page 12
5.0 ELECTRICAL SPECIFICATION page 13
6.0 RECOVERY AND RECYCLING page 13
Page 4
This microscope is a scientic precision instrument designed to last for many years with a minimum of main-
tenance. It is built to high optical and mechanical standards and to withstand daily use.
Optika reminds you that this manual contains important information on safety and maintenance, and that it
must therefore be made accessible to the instrument users.
Optika declines any responsibility deriving from instrument uses that do not comply with this manual.
Safety guidelines
This manual contains important information and warnings regarding safety about installation, use and
maintenance of the microscope. Please read this manual carefully before using the equipment. To
ensure safe use, the user must read and follow all instructions in this manual. OPTIKA products are
designed for safe use in normal operating conditions. The equipment and accessories described in
the manual are manufactured and tested according to industry standards for safety instrumentation
laboratory. Misuse can cause personal injury or damage to the instrument. Keep this manual at hand
close to the instrument, for an easy consultation.
Electrical safety
Before connecting the power cord to wall outlet, ensure that your mains voltage for your region corre-
sponds to the voltage supply of the instrument, and that the illuminator’s switch is in position OFF. The
user must observe the safety regulations in force in his region. The instrument is equipped with CE
safety marking, in any case the user has full responsibility concerning the safe use of that instrument.
Warning/Caution symbols used in this manual
The user should be aware of safety aspects when using the instrument. Warning or hazard symbols
are shown below. These symbols are used in this manual.
Follow the instructions on this symbol to avoid possible severe personal injuries.
Warning of use; the incorrect operation on the instrument can cause damages
to the person or instrument.
Possibility of electric shock.
Attention: high temperature surfaces. Avoid direct contact.
Technical notes or usage tips.
SAFETY GUIDELINES
Page 5
1.0 DESCRIPTION
OBJECTIVE
SLIDE CLAMP
COARSE FOCUSING
KNOB
FINE FOCUSING
KNOB
IRIS DIAPHRAGM
STAGE
NOSEPIECE
EYEPIECES
LIGHT PATH
SELECTOR LEVER
TENSION
ADJUSTMENT
KNOB
DIOPTRIC ADJUSTEMENT
RING
FOCUS STOP KNOB
CONTROL
KEYBOARD
MAIN ON/OFF
SWITCH
LIGHT ADJUSTMENT
KNOB
FIELD DIAPHRAGM
BRIGHTNESS SENSOR
ATTACHMENT
Page 6
1.0 DESCRIPTION
FILTER SLOTS
PHASE RING CENTRING
SCREWS
PHASE CONTRAST
CONDENSER
(B-1000PH MODEL)
CONDENSER-CENTRING
SCREWS X-Y TRANSLATION
KNOBS
CONDENSER
ADJUSTMENT
KNOB
Page 7
2.0 UNPACKING AND ASSEMBLY
2.1 The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the
container and lift the top half of the container. Take some care to avoid that the optical items (objecti-
ves and eyepieces) fall out and get damaged. Using both hands (one around the arm and one around
the base), lift the microscope from the container and put it on a stable desk.
2.2 The objectives are already mounted on the nosepiece or located in individual protective vials (depen-
ding on the model). Remove the objectives from the vials and insert them into the microscope nose-
piece in the order from the lowest magnication to the highest.
2.3 Place the brightness sensor attachment onto the top of the arm and tighten the lock-screw with the
provided wrench. The sensor connector must face the rear of the microscope. Connect the attach-
ment with the provided cable to the socket on the side of the arm.
2.4 Place the observation head onto the top of the attachment and tighten the lock-screw with the provi-
ded wrench. Insert the eyepieces into the eye tubes.
Connect the cable from the provided external power supply into the socket on the back of the base.
The power supply should be used only on network sockets equipped with adequate grounding.
Once positioned and installed with the necessary components, the microscope is ready to be used.
Your microscope is a laboratory instrument designed to last. Handle it always carefully and avoid
abrupt vibrations or shocks. Always disconnect the power supply from the wall socket when not in use
for long time, while you clean it or when you perform any maintenance.
AVOID DISASSEMBLING THE INSTRUMENT
Do not disassemble the instrument. This entails the cancellation of the warranty and may cause mal-
function.
Page 8
3.1 Adjust the observation head
Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then
lock the lock-screw.
3.2 Place the specimen on the stage
Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the specimen is
centred over the stage opening by adjusting the X-Y translation knobs of the stage.
3.3 Lamp settings
The microscope is tted with a 8W high-efciency white led source. Insert the plug of the power supply
into the power socket and turn on the main ON/OFF switch on the side of the main body. Then press
the ON/OFF button on the control keyboard (upper left).
Turn the brightness adjustment knob to a brightness suitable for observation.
3.4 Adjust interpupillary distance
Hold the right and left parts of the observation head with both hands and adjust the interpupillary
distance by turning the two parts until one circle of light can be seen. After nishing the interpupillary
adjustment, turn the dioptric adjustment ring on the left eyetube to the “0” position on the graduated
line.
3.5 Focus and diopter adjustment
Loosen the focus-stop knob, rotate the coarse focusing knob to bring the slide into focus with an
objective with a low magnication. Adjust the ne focusing knob to get the image sharp and clear whi-
le observing with your right eye, then turn the left dioptric adjustment ring to a sharp and clear image
also with the other eye.
Turn the tension-adjust-knob to get a suitable tension for the focus system. When the image is in fo-
cus, turn the nosepiece to choose the objective you need.
3.6 Condenser adjustment
Turn the condenser adjustment knob to move the condenser up or down for a clear and uniform sam-
ple illumination. The two condenser centring screws can be used to centre the condenser.
3.7 Set the numerical aperture
Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator, thus control-
ling image contrast and resolution.
3.8 Field diaphragm
Adjust the eld diaphragm to align the system and to control stray light.
3.9 Additional lters (optional)
The colored lter (green) can be inserted directly above the eld diaphragm. The use of the green lter
is recommended for phase contrast observations.
3.10 Using the polarizing kit (optional)
The polarizing kit consists of two lters: the analyzer and the polarizer. The analyzer is placed in the
lter slot under the head, while the polarizer directly above the eld diaphragm.
3.0 USING THE MICROSCOPE
Page 9
3.0 USING THE MICROSCOPE
3.11 Photo and Video capturing
B-1000 has a dedicated trinocular head that allows to capture images via a photo/video adaptor,
specic for each model of camera. Before taking a picture or lming video, pull out the light path
selector lever (on the right of the optical head).
Please refer to the adaptor and camera manuals for further details.
3.12 Using the phase contrast set
Phase contrast is provided as standard in B-1000PH model, while it’s optional for B-1000BF model.
The phase contrast set has 4 objectives for phase contrast and one condenser with the dedicated
rings of phase. To use the phase contrast, remove the brighteld condenser from the microscope
and replace it with the phase contrast condenser.
In order to obtain a correct phase contrast view, the phase contrast rings must be aligned using the
alignment screws placed on both sides of the condenser.
Follow these steps:
Select an objective by rotating the nosepiece (it’s suggested to start with the 10X
objective), and focus on a slide under brighteld illumination (“BF” or “0” position on
the condenser wheel).
Insert the phase ring to be realigned (by rotating the wheel on the condenser until you read the cor-
responding number, e.g. “10”).
Remove an eyepiece, and replace it with the centring telescope.
Focus the centring telescope, without touching the microscope focus, in order to focus the phase
rings (one bright ring and one darker ring, as picture A).
Turning the two phase ring centring screws, move the bright ring, so that it is completely contained
in the dark ring. (see picture B.)
Remove the telescope, and replace the eyepiece.
Page 10
3.13 Darkeld condenser (optional)
For darkeld observation, the Abbe condenser in standard equipment must be replaced
with a special condenser for darkeld, as showed in the picture.
3.14 Control keyboard
B-1000 illumination can be managed through the keyboard placed on the left of the stand:
1) ON/OFF
4) MEM
2) BOOST
3) AUTO-OFF
5) AUTO
3.0 USING THE MICROSCOPE
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