Gilson CPC Series Manual de usuario

User’s Guide

Gilson CPC Systems

Trademarks

All product and company names are trademarks™ or registered® trademarks of their

respective holders. Use of the trademark(s) in this document does not imply any

aliation with or endorsements by the trademark holder(s).

GILSON CPC SYSTEMS | USER’S GUIDE

TABLE OF CONTENTS

1 | SAFETY

2 | Symbols and Notices

3 | Chemical Hazards

4 | Electrical Hazards

4 | Flammable Solvents

4 | Lifting

4 | Replacement Parts

4 | Signs of Damage

4 | Site Requirements

4 | Spacing

4 | Storage and Movement

5 | INTRODUCTION

6 | Description

8 | Unpacking

10 | Technical Speciﬁcations

14 | Customer Service

15 | INSTALLATION

16 | Drain Pan and Drain Tube Installation

17 | Plumbing Connections

20 | Electrical Connections

21 | OPERATION

22 | Front Panel

25 | Start Up

26 | Control the System

29 | Loop Injection with Manual Valve

30 | Change the Elution Mode (ASC/DSC)

31 | Power Down

33 | MAINTENANCE

34 | Helpful Hints

34 | Cleaning and Decontamination

36 | Maintenance Schedule

37 | Replace a Seal in a Column Rotary Seal

43 | TROUBLESHOOTING

44 | Troubleshooting Table

46 | Error Messages

47 | Repair and Return Policies

49 | WARRANTY

49 | General

50 | Limited Warranty

51 | REPLACEMENT PARTS

AND ACCESSORIES

55 | MATERIALS

55 | Liquid Contact Materials

1GILSON CPC SYSTEMS | USER’S GUIDE

Chapter1|SaFetY

Read this chapter before installing and operating the instrument.

Only trained technical personnel in a laboratory environment may use the instrument for

non-medical, liquid handling purposes. For safe and correct use of the instrument, operating

and service personnel must follow all instructions contained in this guide when installing,

cleaning, and maintaining the instrument. All safety precautions must be observed during all

phases of operation, service, and repair of the instrument.

Failure to comply with these precautions or with warnings described in the user’s guide

violates safety standards of design, manufacture, and intended use of the instrument. Gilson

assumes no liability for customers failing to comply with these requirements.

Read all documentation and safety information for accessories, peripherals, and other

instruments that may be used with this instrument before operating the system.

Refer to the side panel label on the instrument or the Declaration of Conformity document for

the current standards to which the instrument has been found compliant.

IN thIS Chapter

●Symbols and Notices | 2

●Chemical Hazards | 3

●Electrical Hazards | 4

●Flammable Solvents | 4

●Lifting | 4

●Replacement Parts | 4

●Signs of Damage | 4

●Site Requirements | 4

●Spacing | 4

●Storage and Movement | 4

2SAFETY |GILSON CPC SYSTEMS

SAFETY

Symbols and Notices

The following symbols and notices may appear on the instrument or in this document.

Electrical and Hazard Symbols

SYMBOL EXPLANATION

Direct Current

Alternating Current

Protective Conductor Terminal

|Electrical Power ON

OElectrical power OFF

Caution

Caution, Risk of Electric Shock

Caution, Ultraviolet Light, Risk of UV Radiation

Caution, Two Person Lift Required

Warning, Corrosive Chemical

Caution, Hot Surface

3GILSON CPC SYSTEMS | USER’S GUIDE

SAFETY

Label Symbols

The following label symbols may appear on the instrument:

SYMBOL EXPLANATION

Reference of the product

Serial Number

Year of Manufacture

Refer to the User’s Guide

Safety Notices

WARNING

Indicates a potentially hazardous situation which, if not avoided, may result in personal injury.

CAUTION

Indicates a potentially hazardous situation which, if not avoided, may result in minor or

moderate injury.

NOTICE

Indicates a potentially hazardous situation which, if not avoided, may result in equipment

damage.

Chemical Hazards

Any chemicals used for analysis should be handled according to good laboratory

practice (GLP). They should also be stored, used, and disposed of in accordance with the

manufacturer’s specifications, as well as local and national regulations. Potentially hazardous

chemicals can be used with the instrument. Use care when handling chemicals and wear

appropriate personal protective equipment (PPE), such as safety glasses, gloves, etc.

The responsible individual must ensure that personnel are not exposed to hazardous

levels of toxic substances as outlined in the Material Safety Data Sheets (MSDSs), or any

documentation provided by local governing bodies such as The Health Protection Agency

(United Kingdom) or The Occupational Safety and Health Administration (United States).

4SAFETY |GILSON CPC SYSTEMS

SAFETY

Electrical Hazards

Unless specifically instructed, do not remove any protective covers. Detach all sources of

voltage from the instrument before the service, repair, or exchange of parts.

Use only the grounded AC cord provided. Ungrounded power cords can result in electrical

shock and serious personal injury. Faulty or frayed power cords must be immediately

replaced with one of the same type and rating. When it is necessary to use a non-original

power cord, make sure the replacement cord adheres to following specifications and

local building safety codes: 1) European Union Model; Connector A: Male, Type E or F

(Schuko), 16A; Connector B: Female, IEC320/C13, 10A; 250 V~, H05VV-F 3G1.0mm² and

2)UnitedStates and Canadian Model; Connector A: Male Type, NEMA 15-5, 15A; Connector B:

Female, IEC320/C13, 10A; 125 V~, SVT 3x18 AWG.

NOTICE

Use only approved fuses with the speciﬁed current rating. The instrument must be operated

within the voltage speciﬁed on the right panel of the instrument.

Flammable Solvents

Secure all flammable solvents. The temperature of liquids inside the system must be

25°C(77°F) below the lowest flame temperature of any solvents used.

Lifting

The instrument exceeds the weight one person can lift safely. Two or more people are

required to lift the instrument safely. Always lift the instrument from the base and follow any

unpacking instructions provided with the instrument.

Replacement Parts

Be sure to use only replacement parts mentioned in this user’s guide.

Signs of Damage

Do not attempt to use the instrument if there are visible signs of damage.

Site Requirements

Do not operate the instrument if site conditions are not within specifications.

Spacing

Allow sufficient spacing around the system for proper cooling and for the connection of

power cords, plumbing, injection pump, liquid handler, external detectors, or any other

peripherals.

Storage and Movement

Run a clean solvent through the fluid path before storing the instrument. Do not leave buffer

in the system, as it may cause blockages and damage the seals. Rinse the fluid path and

prevent the liquids from flowing out by inlets and outlets before moving the instrument. Store

the instrument indoors (temperature > 5°C) on a flat surface and in an area with low moisture

and low risk of impact or movement.

5GILSON CPC SYSTEMS | USER’S GUIDE

Chapter 1

CHAPTER1|INTRODUCTION

IN THIS CHAPTER

●Description | 6

●Unpacking | 8

●Technical Specifications | 10

●Customer Service | 14

6INTRODUCTION |GILSON CPC SYSTEMS

DESCRIPTION

Description

The Gilson CPC Systems are designed for centrifugal partition chromatography (CPC),

also known as counter‑current chromatography (CCC), a silica‑free,

liquid‑liquid chromatographic (LLC) technique for preparative and industrial‑scale

purifications. Two non‑miscible liquid phases are used: one as the mobile phase eluent and

the other as the stationary phase maintained in the chromatography column by a centrifugal

force. The centrifugal force is created by the rotation of the column, also called the “rotor,”

which is composed of horizontally‑stacked discs.

Gilson CPC Systems can be equipped with several models of columns, which vary in volume

from 100mLto1L.

When used as a standalone unit, an optional kit with manual valves mounted on the right side

of the instrument allows Gilson CPC Systems to change elution mode—ascending(ASC) to

descending(DSC)—and inject sample—load to injection. For more information, refer to Loop

Injection with Manual Valve on page 29 and Change the Elution Mode (ASC/DSC) on page 30.

Injection ranges, typical flow rates, maximumpressures, and rotation speeds vary according

to the column model. Refer to Technical Specifications on page 10.

Gilson CPC Systems are standalone and can be controlled with the built‑in touchscreen and

buttons.

The combination of a Gilson CPC System and PLC Purification System, fully controllable

with Gilson Glider CPC (GGC) Software, creates a complete purification system for various

applications. In this configuration, a detector measures the absorbance and sends the

chromatogram to the onboard control software, after which fractions are collected via the

integrated fraction collector. For more information about PLC Purification Systems, refer to

the PLC2050/2250/2500 Purification System User’sGuide.

Figure 1

Gilson CPC System with PLC Purification System

Este manual sirve para los siguientes modelos

Tabla de contenidos

Otros manuales de Equipo de laboratorio de Gilson

Gilson

Gilson microman e Manual de usuario

Gilson

Gilson SS-15 Manual de usuario

Gilson

Gilson pipetman Manual de usuario

Gilson

Gilson DISTRIMAN Manual de usuario

Gilson

Gilson pipetman P10 Series Manual de usuario

Gilson

Gilson MICROMAN E M25E Manual de usuario

Gilson

Gilson PIPETMAN F Series Manual de usuario

Gilson

Gilson SGA-5RT Manual de usuario

Gilson

Gilson microman e Manual de usuario

Gilson

Gilson Pipetman M Manual de usuario

Gilson

Gilson PIPETMAN P2L Manual de usuario

Gilson

Gilson MICROMAN E M10E Manual de usuario

Gilson

Gilson Vortex Mixer Manual de usuario

Gilson

Gilson PIPETMAN Classic P2 Manual de usuario

Gilson

Gilson DISTRIMAN Manual de usuario

Gilson

Gilson PIPETMAN L Manual de usuario

Gilson

Gilson Trackman Manual de usuario

Gilson

Gilson PLATEMASTER P20 Manual de usuario

Gilson

Gilson ASPEC GX-271 Manual

Gilson

Gilson Safe Aspiration Manual de usuario

Gilson

Gilson Pipetman M Manual de usuario

Gilson

Gilson 223 Manual de usuario

Gilson

Gilson Pipetman Fixed Manual de usuario

Gilson

Gilson PIPETMAN G Manual de usuario

Manuales populares de Equipo de laboratorio de otras marcas

Qiagen

Qiagen DML 3000 Manual de usuario

Queensgate

Queensgate NANOSCAN OP400 Manual de programación

Conviron

Conviron GEN1000-GE Manual de usuario

Parr Instrument

Parr Instrument 4560 Manual de instrucciones

IKA

IKA IC control Manual de usuario

Integra

Integra DOSE IT Manual de usuario

Agilent Technologies

Agilent Technologies 5800 ICP-OES Manual de usuario

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manual de usuario

NI PXI-5422 Manual

Collomix

Collomix Aqix Manual de usuario

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manual de usuario

Ocean Insight

Ocean Insight FLAME-NIR+ Manual de usuario

Parker

Parker ALIGN-MG-NA Manual de usuario

BD 644787 Manual de usuario

DENTAURUM

DENTAURUM Compact Megaplus Manual de usuario

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manual de usuario

VWR

VWR SAS Super IAQ Manual de usuario

illumina

illumina MiSeqDx Manual de usuario