Gilson MICROMAN E M25E Manual de usuario

User’s Guide

TABLE OF CONTENTS

1TABLE OF CONTENTS |MICROMAN E®

TABLE OF CONTENTS

INTRODUCTION | 2

Parts Checklist | 3

Good Laboratory Practice (GLP) Features | 3

Description | 4

Operating Ranges and Material | 5

Speciﬁcations | 6

FITTING THE CAPILLARY PISTON | 8

PIPETTING | 10

Setting the Volume | 10

Aspirating | 11

Dispensing | 11

Ejecting the Capillary Piston | 12

TROUBLESHOOTING | 13

CLEANING AND DECONTAMINATION | 14

WARRANTY | 17

Before use, please carefully read

WARRANTY on page 17 of this

User’s Guide!

INTRODUCTION

MICROMAN E® |INTRODUCTION

TABLE OF CONTENTS |MICROMAN E®

Chapter 1

INTRODUCTION

Congratulations on purchasing your new

MICROMAN E®pipette. MICROMAN E is suitable

for many different applications, including

pipetting volatile, viscous, dense, or high surface

tension liquids. It is a quality product from Gilson,

which is fully compliant with ISO 8655 standards.

The six models available allow for precise and

accurate pipetting of viscous, dense, and volatile

liquids. They also enable contamination-free

pipetting, preventing vapor contamination and

cross-contamination. M25E, M50E, and M250E,

with thin and tall capillary pistons (CPs) are

especially suited for use with tall or narrow

vessels.

Enjoy the following features:

●MICROMAN E pipettes are equipped with a

positive displacement mechanism isolating

the aspirated liquid from the pipette body.

The positive displacement mechanism

prevents the sample-to-sample contamination

that can result from the aerosol effect.

●MICROMAN E uses disposable capillary

pistons. These are the only parts that contact

with the aspirated liquids, and replacement

thereof will provide absolute protection

against contamination caused by carryover

between samples or reagents. Simply replace

the capillary piston between assays; they are

ejected automatically and simultaneously,

thus avoiding any risk for the operator.

●MICROMAN E is equipped with a direct

reading volumeter that allows precise

adjustment of the required volume. At the

manufacturing stage, each MICROMAN E is

permanently adjusted to the corresponding

capillary piston. The capillary piston is

positioned automatically, and therefore

no further adjustment is requested when

replacing it.

●MICROMAN E requires no lubrication, due to

the nature and quality of the materials used in

its component parts.

INTRODUCTION

3INTRODUCTION | MICROMAN E®

Parts Checklist

Verify that the following items are present:

●MICROMAN E,

●User’s Guide,

●Safety bag,

●Adhesive ID-tags (strip of 6),

●Capillary pistons (10),

●Certificate of conformity

(including barcode sticker).

Good Laboratory Practice (GLP)

Features

●No need to touch disposables

(capillary piston).

●Serial Number: engraved on

pipette body.

●Barcode: on the box and

with the certificate (can be

transferred).

●ID Tag (Application or User).

●Color-coded push button

indicating the volume range.

●Data matrix engraved on

pipette body.

●Gilson certificate of

conformity according

to ISO 8655.

Figure 1

Data Matrix and

Serial Number

Location

INTRODUCTION

MICROMAN E® |USER’S GUIDE

INTRODUCTION | MICROMAN E®

Description

Color-coded push button

indicating the volume

range for aspirating and

dispensing.

Body or handle

Capillary

Piston

Shaft

Thumbwheel for

setting the volume

Volumeter

Volume unlock button

Data matrix code

Figure 2

MICROMAN® E

Operating Ranges and Material

Available Models

Materials

INTRODUCTION

5INTRODUCTION | MICROMAN E®

MODEL VOLUME RANGE

M10E 1 µL to 10 µL

M25E 3 µL to 25 µL

M50E 20 µL to 50 µL

M100E 10 µL to 100 µL

M250E 50 µL to 250 µL

M1000E 100 µL to 1000 µL

MODEL

SPRING

CLAMP SHAFT BODY (B)

M10E SS Be Alloy PBT PVDF/PP

M25E SS Be Alloy PVDF PVDF/PP

M50E SS Be Alloy PVDF PVDF/PP

M100E SS Be Alloy PBT PVDF/PP

M250E SS Be Alloy PVDF PVDF/PP

M1000E SS Be Alloy PVDF PVDF/PP

CP CAPILLARY (C) PISTON (P)

CP10 Polypropylene Polyester

CP25 Polypropylene Polyester

CP50 Polypropylene Polyester

CP100 Polypropylene Polyethylene

CP250 Polypropylene Polyethylene

CP1000 Polypropylene Polyacetal

Abbreviations

SS = Stainless Steel

Be = Beryllium

PBT = Polybutylene Terephthalate

PP = Polypropylene

PVDF = Polyvinylidene Fluoride

INTRODUCTION

MICROMAN E® |USER’S GUIDE

INTRODUCTION | MICROMAN E®

Speciﬁcations

MICROMAN E is a high quality pipette that offers

excellent accuracy and precision. The figures

provided in the “ Gilson Maximum Permissible

Errors ” table were obtained using Gilson CPs.

These figures are only guaranteed when using

genuine Gilson CPs.

Each pipette is inspected and validated by qualified

technicians according to the Gilson Quality System.

Gilson declares that its manufactured pipettes

comply with the requirements of the ISO 8655

Standard, by type testing. The adjustment is carried

out under strictly defined and monitored conditions

(ISO 8655-6):

●Basis of adjustment, Ex.

●Reference temperature, 20°C

●Relative humidity, 50%

●Barometric pressure, 101 kPa

●Use of distilled water grade 3 (ISO 3696)

●Ten measurements for each test volume,

which are nominal volume, 50% of nominal

volume, and the minimum or 10% of nominal

volume.

Performance Tests

Each pipette is inspected and validated according

to the Gilson Quality Assurance System. Based

on extensive historical data, manufacturing

conditions, and expertise, and in compliance with

ISO standards relative to statistical process control,

the assurance level of this instrument performing

to specifications is 99.8 %. Specifications rely on

quality and consistency for the whole pipetting

system and are guaranteed only when the pipette is

used with Gilson capillary pistons.

MAXIMUM PERMISSIBLE ERRORS

MODEL

(PART

NUMBER)

VOLUME

(

GILSON ISO 8655

SYSTEMATIC

ERROR

(µL)

RANDOM

ERROR

(µL)

SYSTEMATIC

ERROR

(µL)

RANDOM

ERROR

(µL)

M10E

(FD10001)

Min. 1 ±0.09 ≤0.03 ±0.2 ≤0.1

5 ±0.10 ≤0.03 ±0.2 ≤0.1

Max. 10 ±0.15 ≤0.06 ±0.2 ≤0.1

M25E

(FD10002)

Min. 3 ±0.25 ≤0.08 ±0.7 ≤0.3

10 ±0.27 ≤0.08 ±0.7 ≤0.3

Max. 25 ±0.30 ≤0.10 ±0.7 ≤0.3

M50E

(FD10003)

Min. 20 ±0.34 ≤0.20 ±0.7 ≤0.3

Max. 50 ±0.70 ≤0.30 ±0.7 ≤0.3

M100E

(FD10004)

Min. 10 ±0.50 ≤0.20 ±1.5 ≤0.6

50 ±0.75 ≤0.30 ±1.5 ≤0.6

Max. 100 ±1.00 ≤0.40 ±1.5 ≤0.6

M250E

(FD10005)

Min. 50 ±1.50 ≤0.30 ±6 ≤2.0

100 ±1.70 ≤0.30 ±6 ≤2.0

Max. 250 ±2.50 ≤0.50 ±6 ≤2.0

M1000E

(FD10006)

Min. 100 ±3.0 ≤1.6 ±12 ≤4.0

500 ±5.0 ≤2.5 ±12 ≤4.0

Max. 1000 ±8.0 ≤4.0 ±12 ≤4.0

INTRODUCTION

7INTRODUCTION | MICROMAN E®

Gilson Maximum Permissible Errors

The data given in the table conform to the

ISO 8655-2 Standard.

MAXIMUM PERMISSIBLE ERRORS

MODEL

(PART

NUMBER)

VOLUME

(

GILSON ISO 8655

SYSTEMATIC

ERROR

(µL)

RANDOM

ERROR

(µL)

SYSTEMATIC

ERROR

(µL)

RANDOM

ERROR

(µL)

M10E

(FD10001)

Min. 1 ±0.09 ≤0.03 ±0.2 ≤0.1

5 ±0.10 ≤0.03 ±0.2 ≤0.1

Max. 10 ±0.15 ≤0.06 ±0.2 ≤0.1

M25E

(FD10002)

Min. 3 ±0.25 ≤0.08 ±0.7 ≤0.3

10 ±0.27 ≤0.08 ±0.7 ≤0.3

Max. 25 ±0.30 ≤0.10 ±0.7 ≤0.3

M50E

(FD10003)

Min. 20 ±0.34 ≤0.20 ±0.7 ≤0.3

Max. 50 ±0.70 ≤0.30 ±0.7 ≤0.3

M100E

(FD10004)

Min. 10 ±0.50 ≤0.20 ±1.5 ≤0.6

50 ±0.75 ≤0.30 ±1.5 ≤0.6

Max. 100 ±1.00 ≤0.40 ±1.5 ≤0.6

M250E

(FD10005)

Min. 50 ±1.50 ≤0.30 ±6 ≤2.0

100 ±1.70 ≤0.30 ±6 ≤2.0

Max. 250 ±2.50 ≤0.50 ±6 ≤2.0

M1000E

(FD10006)

Min. 100 ±3.0 ≤1.6 ±12 ≤4.0

500 ±5.0 ≤2.5 ±12 ≤4.0

Max. 1000 ±8.0 ≤4.0 ±12 ≤4.0

FITTING THE CAPILLARY PISTON

MICROMAN E® |USER’S GUIDE

INTRODUCTION | MICROMAN E®

Chapter 2

FITTING THE CAPILLARY

PISTON

Make sure the capillary piston

(CP) is compatible with the

MICROMAN E model that will

be used.

CP25, CP50, and CP250 Bulk

The capillaries and pistons

are delivered in two separate

boxes. To fit a capillary piston:

1. Take care not to

damage the sealing

tip when handling

the piston .

2. Select a piston

and slide it into the

capillary .

3. Gently push the

capillary until it snaps

onto the capillary

holder.

4. Attach the stem (

by slowly pressing

the push button until

you feel and hear a

slight click and then

continue to press to

the first stop. Finally,

pipette the liquid.

CP MODEL

CP10 M10E

CP25 M25E

CP50 M50E

CP100 M100E

CP250 M250E

CP1000 M1000E

Figure 3

MICROMAN

Capillary Piston

Never lubricate the capillary holder,

capillary, or any other part of the

pipette. If the capillary tends to slip

o the capillary holder, clean the

capillary holder with ethanol, using

a medical wipe or similar soft tissue.

FITTING THE CAPILLARY PISTON

9FITTING THE CAPILLARY PISTON | MICROMAN E®

CP10, CP25, CP50, CP100, CP250, and

CP1000 Tipack

Capillary pistons (CP) are delivered ready to use.

To fit a capillary piston:

1. Press the

MICROMAN E onto

the capillary piston

until it is firmly

seated .

2. Pick up the CP

from the rack .

3. Slowly press the

push button until

you feel and hear

a slight click and

continue to press

to the first stop .

Then pipette the

liquid.

4. Press the push button

on the second stop to

eject the CP.

Figure 4

CP fitting on MICROMAN®E

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