Gilson Microman e Manual de usuario

User’s Guide

2TABLE OF CONTENTS | MICROMAN® E

TABLE OF CONTENTS

INTRODUCTION | 3

Parts Checklist | 4

Good Laboratory Practice (GLP) Features | 4

Description | 5

Operating Ranges and Material | 6

Speciﬁcations | 7

FITTING THE CAPILLARY PISTON | 9

PIPETTING | 11

Setting the Volume | 11

Aspirating | 12

Dispensing | 13

Ejecting the Capillary Piston | 13

TROUBLESHOOTING | 14

CLEANING AND DECONTAMINATION | 15

WARRANTY | 18

Before use, please carefully read

WARRANTY on page 17 of this

User’s Guide!

TABLE OF CONTENTS

INTRODUCTION

MICROMAN® E |USER’S GUIDE

TABLE OF CONTENTS | MICROMAN® E

Chapter 1

INTRODUCTION

The ergonomically designed MICROMAN® E

positive-displacement pipettes, along with

disposable capillary piston (CP) tips, provide

the highest precision when pipetting viscous,

volatile, or non-aqueous liquids. Capillary pistons

eliminate the air cushion between the sample and

disposable piston, ensuring the accuracy of the

pipette is not affected by the physical properties

of the sample.

Positive-displacement technology is the ideal

solution to prevent from aerosol contamination

or sample carry-over, thus protecting the pipette,

the user, and the sample.

MICROMAN E is available as variable volume

single-channel models, with six models covering

a volume range from 1 to 1000 µL. M25E, M50E,

and M250E, with thin and tall capillary pistons

(CPs) are especially suited for use with tall or

narrow vessels.

Designed with comfort in mind, MICROMAN E is

a must for every workflow and application thanks

to its main features:

●QuickSnap system for a direct fitting of the

CPs: fitting is as easy as fitting a tip on an air

displacement pipette

●Improved ergonomic handle shape that rests

comfortably in hand

●Volume control switch to avoid any

accidental volume change when pipetting

●Complete and reliable pipetting of

challenging fluids: performances remain

unaffected by the physical properties of the

fluids, or at different temperatures

●MICROMAN E and CPs are the strongest

barrier against residual sample

carryover, aerosol contamination, and

cross-contamination. They provide a triple

protection: you, your sample, and your

pipette are protected.

INTRODUCTION

4INTRODUCTION | MICROMAN® E

Parts Checklist

Verify that the following items are present:

●MICROMAN E,

●User’s Guide,

●Safety bag,

●Adhesive ID-tags (strip of 6),

●Capillary pistons (10),

●Certificate of conformity

(including barcode sticker).

Good Laboratory Practice (GLP)

Features

●No need to touch disposables

(capillary piston).

●Serial Number: engraved on

pipette body.

●Barcode: on the box and

with the certificate (can be

transferred).

●ID Tag (Application or User).

●Color-coded push button

indicating the volume range.

●Data matrix engraved on

pipette body.

●Gilson certificate of

conformity according

to ISO 8655.

Figure 1

Data Matrix and

Serial Number

Location

INTRODUCTION

MICROMAN® E |USER’S GUIDE

INTRODUCTION | MICROMAN® E

Description

Color-coded push button

indicating the volume

range for aspirating and

dispensing.

Body or handle

Capillary

Piston

Shaft

Thumbwheel for

setting the volume

Volumeter

Volume control switch

Data matrix code

Figure 2

MICROMAN® E Description

INTRODUCTION

6INTRODUCTION | MICROMAN® E

Operating Ranges and Material

Available Models

Materials

Model Part

Number

Capillary

Pistons Volume Range

M10E FD10001 CP10/CP10ST 1 µL to 10 µL

M25E FD10002 CP25/CP25ST 3 µL to 25 µL

M50E FD10003 CP50/CP50ST 20 µL to 50 µL

M100E FD10004 CP100/CP100ST 10 µL to 100 µL

M250E FD10005 CP250/CP250ST 50 µL to 250 µL

M1000E FD10006 CP1000/CP1000ST 100 µL to 1000 µL

MICROMAN® E

Model Spring

(A)

Clamp

(B)

Shaft

(C)

Body

(D)

M10E SS Be Alloy PBT PVDF/PP

M25E SS Be Alloy PVDF PVDF/PP

M50E SS Be Alloy PVDF PVDF/PP

M100E SS Be Alloy PBT PVDF/PP

M250E SS Be Alloy PVDF PVDF/PP

M1000E SS Be Alloy PVDF PVDF/PP

CAPILLARY PISTONS

Model Capillary

(E)

Piston

(F)

CP10 PP PES

CP25 PP PES

CP50 PP PES

CP100 PP PE

CP250 PP PE

CP1000 PP POM

Abbreviations

SS = Stainless Steel

Be = Beryllium

PBT = Polybutylene Terephthalate

PP = Polypropylene

PVDF = Polyvinylidene Fluoride

PES = Polyester

PE = Polyethylene

POM = Polyacetal

NOTE

For more information on chemical

resistance of plastic materials, please refer

to the LT800550 Gilson Guide to Pipetting -

Appendix E, on www.gilson.com.

INTRODUCTION

MICROMAN® E |USER’S GUIDE

INTRODUCTION | MICROMAN® E

Speciﬁcations

MICROMAN E is a high quality pipette that offers

excellent accuracy and precision. The figures

provided in the Gilson Maximum Permissible Errors

on page 8, table were obtained using Gilson CPs.

These figures are only guaranteed when using

genuine Gilson CPs.

Each pipette is inspected and validated by qualified

technicians according to the Gilson quality system.

Gilson declares that its manufactured pipettes

comply with the requirements of the ISO 8655

standard, by type testing. The adjustment is carried

out under strictly defined and monitored conditions

(ISO 8655-6):

●Basis of adjustment, Ex.

●Reference temperature, 20°C

●Relative humidity, 50%

●Barometric pressure, 101 kPa

●Use of distilled water grade 3 (ISO 3696)

●Ten measurements for each test volume,

which are nominal volume, 50% of nominal

volume, and the minimum or 10% of nominal

volume.

Performance Tests

Each pipette is inspected and validated according

to the Gilson quality assurance system. Based

on extensive historical data, manufacturing

conditions, and expertise, and in compliance with

ISO standards relative to statistical process control,

the assurance level of this instrument performing

to specifications is 99.8%. Specifications rely on

quality and consistency for the whole pipetting

system and are guaranteed only when the pipette is

used with Gilson capillary pistons.

INTRODUCTION

8INTRODUCTION | MICROMAN® E

Gilson Maximum Permissible Errors

MICROMAN® E

Nom.

Vol.

(µL)

Vol.

(µL)

Vol.

(%)

Maximum Permissible Errors

Gilson ISO 8655-2 (Table 3)

Systematic

Error

(μL)

Random

Error

(μL)

Systematic

Error

(%)

Random

Error

(CV)*

Systematic

Error

(μL)

Random

Error

(μL)

Systematic

Error

(%)

Random

Error

(CV)*

M10E (P/N FD10001) WITH CP10 AND CP10ST

100

± 0.090

± 0.100

± 0.150

≤0.030

≤0.060

± 9.0

± 2.0

± 1.5

≤3.00

≤0.60

± 0.200

≤0.100

± 20

± 4.0

± 2.0

≤10

≤2.0

≤1.0

M25E (P/N FD10002) WITH CP25 AND CP25ST

12.0

40.0

100

± 0.25

± 0.27

± 0.30

≤0.080

≤0.100

± 8.3

± 2.7

± 1.2

≤2.67

≤0.80

≤0.40

± 0.35

≤0.15

± 11.67

± 3.5

± 1.4

≤5.0

≤1.5

≤0.60

M50E (P/N FD10003) WITH CP50 AND CP50ST

50 20

40.0

100

± 0.34

± 0.70

≤0.20

≤0.30

± 1.7

± 1.4

≤1.00

≤0.60

± 0.70

≤0.30

± 3.5

± 1.4

≤1.5

≤0.60

M100E (P/N FD10004) WITH CP100 AND CP100ST

100

± 0.50

± 0.75

± 1.00

≤0.20

≤0.30

≤0.40

± 5.0

± 1.5

± 1.0

≤2.00

≤0.60

≤0.40

± 1.4

≤0.6

± 14

± 2.8

± 1.4

≤6.0

≤1.2

≤0.60

M250E (P/N FD10005) WITH CP250 AND CP250ST

250

100

250

20.0

40.0

100

± 1.50

± 1.70

± 2.50

≤0.30

≤0.50

± 3.0

± 1.7

± 1.0

≤0.60

≤0.30

≤0.20

± 3.0

≤1.0

± 6.0

± 3.0

± 1.2

≤2.0

≤1.0

≤0.40

M1000E (P/N FD10006) WITH CP1000 AND CP1000ST

1000

100

500

1000

100

± 3.0

± 5.0

± 8.0

≤1.6

≤2.5

≤4.0

± 3.0

± 1.0

± 0.8

≤1.60

≤0.50

≤0.40

± 12

≤4.0

± 12

± 2.4

± 1.2

≤4.0

≤0.80

≤0.40

*CV means Coecient of Variation (%)

NOTE

With a precise pipetting technique, the M25E

model can be used to aspirate volumes as low

as 2.5 µL (10% of the nominal volume of the

pipette).

FITTING THE CAPILLARY PISTON

MICROMAN® E |USER’S GUIDE

INTRODUCTION | MICROMAN® E

CP10, CP25, CP50, CP100, CP250, and

CP1000 TIPACK

Capillary pistons (CP) are delivered ready to use.

To fit a capillary piston:

1. Press the MICROMAN E onto the capillary

piston until it is firmly seated 1.

2. Pick up the CP from the rack 2.

3. Slowly press the push button until you feel

and hear a slight click and continue to press

to the first stop 3.

4. Pre-wet the CP, and then aspirate the liquid.

5. Press the push button on the second stop to

eject the CP 3.

Chapter 2

FITTING THE CAPILLARY

PISTON

Make sure the capillary piston (CP) is compatible

with the MICROMAN E model that will be used.

MICROMAN® E

Nom.

Vol.

(µL)

Vol.

(µL)

Vol.

(%)

Maximum Permissible Errors

Gilson ISO 8655-2 (Table 3)

Systematic

Error

(μL)

Random

Error

(μL)

Systematic

Error

(%)

Random

Error

(CV)*

Systematic

Error

(μL)

Random

Error

(μL)

Systematic

Error

(%)

Random

Error

(CV)*

M10E (P/N FD10001) WITH CP10 AND CP10ST

100

± 0.090

± 0.100

± 0.150

≤0.030

≤0.060

± 9.0

± 2.0

± 1.5

≤3.00

≤0.60

± 0.200

≤0.100

± 20

± 4.0

± 2.0

≤10

≤2.0

≤1.0

M25E (P/N FD10002) WITH CP25 AND CP25ST

12.0

40.0

100

± 0.25

± 0.27

± 0.30

≤0.080

≤0.100

± 8.3

± 2.7

± 1.2

≤2.67

≤0.80

≤0.40

± 0.35

≤0.15

± 11.67

± 3.5

± 1.4

≤5.0

≤1.5

≤0.60

M50E (P/N FD10003) WITH CP50 AND CP50ST

50 20

40.0

100

± 0.34

± 0.70

≤0.20

≤0.30

± 1.7

± 1.4

≤1.00

≤0.60

± 0.70

≤0.30

± 3.5

± 1.4

≤1.5

≤0.60

M100E (P/N FD10004) WITH CP100 AND CP100ST

100

± 0.50

± 0.75

± 1.00

≤0.20

≤0.30

≤0.40

± 5.0

± 1.5

± 1.0

≤2.00

≤0.60

≤0.40

± 1.4

≤0.6

± 14

± 2.8

± 1.4

≤6.0

≤1.2

≤0.60

M250E (P/N FD10005) WITH CP250 AND CP250ST

250

100

250

20.0

40.0

100

± 1.50

± 1.70

± 2.50

≤0.30

≤0.50

± 3.0

± 1.7

± 1.0

≤0.60

≤0.30

≤0.20

± 3.0

≤1.0

± 6.0

± 3.0

± 1.2

≤2.0

≤1.0

≤0.40

M1000E (P/N FD10006) WITH CP1000 AND CP1000ST

1000

100

500

1000

100

± 3.0

± 5.0

± 8.0

≤1.6

≤2.5

≤4.0

± 3.0

± 1.0

± 0.8

≤1.60

≤0.50

≤0.40

± 12

≤4.0

± 12

± 2.4

± 1.2

≤4.0

≤0.80

≤0.40

Figure 3

CP fitting on MICROMAN® E

Click: The CP is attached

Press to the 1st stop: the

pipette is ready to aspirate

Press to the 2nd stop: the

CP is ejected

NOTE

Pre-wetting the CP before pipetting helps

prepare the CP for the best pipetting

performance. Pre-wetting the CP helps ensure

that volumes that you pipette will achieve

accuracy and precision within speciﬁcations.

FITTING THE CAPILLARY PISTON

10 FITTING THE CAPILLARY PISTON | MICROMAN® E

CP25, CP50, and CP250 Bulk

The capillaries and pistons are delivered in two

separate boxes.

To fit a capillary piston:

1. Take care not to

damage the sealing

tip 1when handling

the piston 3.

2. Select a piston 3

and slide it into the

capillary 4.

3. Gently push the

capillary until it snaps

onto the capillary

holder.

4. Attach the stem 2

by slowly pressing

MICROMAN E’s push

button until you feel

and hear a slight click

and then continue to

press to the first stop.

5. Pre-wet the CP, and

then aspirate the

liquid.

Figure 4

MICROMAN® E

Capillary Piston

Never lubricate the capillary holder,

capillary, or any other part of the

pipette. If the capillary tends to slip

o the capillary holder, clean the

capillary holder with ethanol, using

a medical wipe or similar soft tissue.

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