Gilson Pipetman Fixed Manual de usuario

User’s Guide

FIXED

TABLE OF CONTENTS

TABLE OF CONTENTS |PIPETMAN® FIXED

TABLE OF CONTENTS

INTRODUCTION | 3

SPECIFICATIONS | 4

PIPETTING | 6

Aspirate | 6

Dispense | 6

Pre-Rinse | 7

User Adjustment | 7

ASSOCIATED TIPS | 9

RECOMMENDATIONS | 11

TROUBLESHOOTING | 12

SPARE PARTS | 14

WARRANTY | 15

Chapter 1

INTRODUCTION

PIPETMAN

Fixed volume

pipettes are available in

14 dierent models.

Together, they cover a

range of volumes from 2 to

5000 µL.

The pipetting mechanism

incorporates a highly

polished stainless steel

piston, a seal, and an O-ring.

The mechanism requires no

routine lubrication.

Tip ejector and disposable

tips mean no need to handle

tips, protecting the user from

contamination.

The tip holder, the

connecting nut, and the tip

ejector are auto,clavable for

20 minutes at 121°C and

0.1 MPa pressure.

The PIPETMAN Fixed

can be easily re-adjusted

in the laboratory, with

compensation for

variations in ﬂuid viscosity

and density. A special

calibration key

is provided.

The nominal volume of

each model is shown on

the small label on the

push button.

PIPETMAN Fixed is in

accordance with ISO 8655

Standard.

INTRODUCTION

PIPETMAN® FIXED |USER’S GUIDE

Figure 1

PIPETMAN® Fixed

Tip ejector

button

Push button

Body

handle

Connecting

nut

Tip holder

Tip ejector

PIPETMAN

DIAMOND tip

Figure 2

Push Button

Top View

SPECIFICATIONS

4SPECIFICATIONS | PIPETMAN® FIXED

Chapter 2

SPECIFICATIONS

Maximum Permissible Errors

PIPETMAN Fixed is a high quality pipette that

oers excellent accuracy and precision. The ﬁgures

given in the “Gilson Maximum Permissible Errors”

table were obtained using “PIPETMAN DIAMOND

Tips”. These ﬁgures are only guaranteed using

genuine PIPETMAN DIAMOND Tips.

MODEL VO L*

GILSON ISO 8655

SYSTEMATIC

ERROR*

RANDOM

ERROR*

SYSTEMATIC

ERROR*

RANDOM

ERROR*

(F123770) 2± 0.08 ≤0.03 ± 0.08 ≤0.04

(F123771) 5± 0.10 ≤0.04 ± 0.125 ≤0.075

F10

(F123772) 10 ± 0.10 ≤0.05 ± 0.12 ≤0.08

F20

(F123604) 20 ± 0.20 ≤0.06 ± 0.2 ≤0.1

F25

(F123775) 25 ± 0.25 ≤0.07 ± 0.5 ≤0.2

F50

(F123778) 50 ± 0.40 ≤0.15 ± 0.5 ≤0.2

F100

(F123784) 100 ± 0.80 ≤0.25 ± 0.8 ≤0.3

F200

(F123605) 200 ± 1.60 ≤0.30 ± 1.6 ≤0.6

F250

(F123787) 250 ± 3.00 ≤0.75 ± 4.0 ≤1.5

F300

(F123788) 300 ± 3.50 ≤0.75 ± 4.0 ≤1.5

F400

(F123789) 400 ± 3.60 ≤0.80 ± 4.0 ≤1.5

F500

(F123790) 500 ± 4.00 ≤1.00 ± 4.0 ≤1.5

F1000

(F123606) 1000 ± 8.0 ≤1.3 ± 8.0 ≤3.0

F5000

(F123607) 5000 ± 30 ≤8 ± 40 ≤15.0

NOTE

The data given in the table conform to the

ISO 8655-2 Standard.

*All Values in Microliters

SPECIFICATIONS

PIPETMAN® FIXED |USER’S GUIDE

SPECIFICATIONS | PIPETMAN® FIXED

Each pipette is inspected and validated by

qualiﬁed technicians according to the Gilson

Quality System.

Gilson declares that its manufactured pipettes

comply with the requirements of the ISO 8655

Standard, by type testing. The adjustment is

carried out under strictly deﬁned and monitored

conditions (ISO 8655-6):

●Basis of adjustment, Ex.

●Reference temperature, 20°C

●Relative humidity, 50%

●Barometric pressure, 101 kPa

●Use of distilled water

grade 3 (ISO 3696)

●Ten measurements for each test volume.

PIPETTING

6PIPETTING | PIPETMAN® FIXED

NOTE

Always ﬁt a tip to your PIPETMAN before

aspirating any liquid.

Chapter 3

PIPETTING

Place a tip on the tip holder. Press the tip on ﬁrmly

using a slight twisting motion to ensure a positive,

airtight seal (refer to CHAPTER 4 - ASSOCIATED

TIPS, on page 9).

Aspirate

●Press the push button to

the first positive stop (A),

refer to Figure 3.

●Hold the pipette vertically

and immerse the tip

into the liquid (depth

immersion 2-4 mm).

●Release the push button

slowly and smoothly to

aspirate the liquid (B),

refer to Figure 3.

●Wait one second and then

withdraw the tip from the

liquid. Wipe any droplets

away from the outside of

the tip using medical wipe.

Dispense

●Place the end of the tip against the inside wall

of the vessel at an angle of 10 to 40 degrees.

Press the push button smoothly to the first

stop (C), refer to Figure 3. Wait one second.

Press the push button to the second stop to

expel any residual liquid from the tip (D), refer

to Figure 3.

●Keep the push button pressed fully down

and (while removing the pipette) draw the tip

along the inside surface of the vessel.

●Release the push button smoothly.

●Eject the tip by pressing the tip ejector button.

Figure 3

Pipetting Positions

NOTE

Avoid touching the oriﬁce of the tip.

A B C

PIPETTING

PIPETMAN® FIXED |USER’S GUIDE

PIPETTING | PIPETMAN® FIXED

NOTE

Always ﬁt a tip to your PIPETMAN before

aspirating any liquid.

User Adjustment

PIPETMAN Fixed is factory calibrated using

distilled water and very high precision balances.

The nominal value of the PIPETMAN Fixed may

be slightly adjusted to compensate for liquids of

dierent density or viscosity.

This adjustment to accommodate for density

or viscosity is limited to one full turn of the

calibration key in either direction, which equals:

± 1.0 µL (models F2 to F20)

± 4.0 µL (models F25 to F100)

± 10.0 µL (models F120 to F200)

± 40.0 µL (models F250 to F1000)

± 200.0 µL (model F5000)

Adjustments of the factory

calibration must only be performed

using a special calibration key (refer

to Figure 4, on page 8)

NOTICE

NOTE

The display is graduated in tenths of

the key revolution (letters A, B,... J) and

is read from left to right. Each small

graduation mark represents 0.2 of the

distance between each letter.

Pre-Rinse

Some liquids (e.g., protein–containing solutions

and organic solvents) can leave a ﬁlm of liquid on

the inside the wall of the tip; pre-rinse the tip to

minimize any errors that may be related to this

phenomenon.

Pre-rinsing consists of aspirating the ﬁrst volume

of liquid and then dispensing it back into the same

vessel (or to waste). Subsequent volumes that you

pipette will have levels of accuracy and precision

within speciﬁcations.

This pre-rinsing operation should be repeated

when a new tip is used.

PIPETTING

8PIPETTING | PIPETMAN® FIXED

To adjust the volume setting to

compensate for a speciﬁc density

or viscosity, slide the key over

the push button until notches ﬁt

into the grooves in the calibration

sleeve (Figure 4), and then turn

the key slowly:

●Clockwise to decrease the

volume.

●Counterclockwise to

increase the volume; go

above the desired value,

then decrease the volume

to reach the required

setting.

Example: When using a PIPETMAN F100 to

aspirate a particularly viscous solution, you

may determine gravimetrically that the volume

delivered is 98.8 µL, and the display reads

“E 0.2”. Therefore, you wish to increase the

volume dispensed by 1.2 µL. As the interval

between each letter on the calibration display

represents 0.4 µL for the F100 (1/10 of 4 µL), the

calibration tool must be turned three units until

the display reads “B 0.2”.

It is possible to alter the volume by more than

one full turn in the counterclockwise direction. In

this case, remember to readjust the same number

of turns when it is necessary to return to the

original setting.

Check the new volume gravimetrically. If the

volumes delivered are still not suciently close to

the desired value, make another slight adjustment

in the relevant direction.

Be sure to change tips between

volume setting adjustments and

to pre-rinse each new tip.

When the PIPETMAN Fixed is

readjusted to compensate for a

particular solution, the original

setting (ex. 0.2) can be noted on

the self-adhesive labels supplied

(Figure 5).

Figure 4

Volume Setting

Adjustment

Figure 5

Self-Adhesive

Labels

NOTE

Liquids of high density, vapor

pressure, or viscosity are best

pipetted by the Gilson MICROMAN®

positive displacement pipette.

ASSOCIATED TIPS

PIPETMAN® FIXED |USER’S GUIDE

PIPETTING | PIPETMAN® FIXED

Chapter 4

ASSOCIATED TIPS

PIPETMAN® DIAMOND Tips are made to the

highest speciﬁcations and strict quality control is

maintained throughout the manufacturing process.

These tips are used to calibrate PIPETMAN Fixed,

therefore for optimum performance we

recommend using PIPETMAN DIAMOND Tips with

your PIPETMAN Fixed.

To ensure accuracy and precision, Gilson's Quality

Assurance System focuses on the following critical

parameters:

●PIPETMAN DIAMOND Tips are made from

pure polypropylene (virgin, metal-free, to

avoid the possibility of contamination).

They are available sterilized and with filters.

●Optimized shape (revised collar for

optimum sealing, thin walls, and fine point),

making them easy to mount, flexible, with

no vortexing, and improved precision.

●PIPETMAN DIAMOND Tips are free from

even microscopic defects, especially

at the orifice. All surfaces are smooth

and hydrophobic, thereby avoiding the

excessive retention of liquids that causes

poor accuracy and a lack of precision.

●Mold and cavity

references are marked on

the collar, ensuring the

traceability. For quality

assurance purposes,

batch numbers appear on

all packages (bags and

boxes).

●They form an air-tight

seal with the tip holder,

preventing the leaks that

cause poor accuracy and

a lack of precision.

●PIPETMAN DIAMOND Tips (except filter

tips) may be autoclaved at 121°C for

20 minutes at 0.1 MPa.

NOTE

Liquids of high density, vapor

pressure, or viscosity are best

pipetted by the Gilson MICROMAN®

positive displacement pipette.

Figure 6

PIPETMAN

DIAMOND Tips

Traceability

ASSOCIATED TIPS

10 ASSOCIATED TIPS | PIPETMAN® FIXED

PIPETMAN DIAMOND Filter Tips

Filter tips are used when sample-to-sample,

sample-to-pipette, or sample-to-operator

contamination must be avoided.

Gilson's sterilized PIPETMAN DIAMOND Filter

Tips are certiﬁed free of detectable RNase,

DNase, DNA, RNA, and protease.

PIPETTE PIPETMAN DIAMOND TIPS TYPE

F2 to F100 D200 Regular

F200 D200/D300 Regular

F250 to F1000 D1000 Regular

F5000 D5000 Regular

PIPETTE PIPETMAN DIAMOND TIPS TYPE

F2 to F25 DF30ST Sterilized

F50 to F100 DF100ST Sterilized

F200 DF200ST/DF300ST Sterilized

F250 to F1000 DF1000ST Sterilized

To ensure the best performance

from your Gilson pipette, you

shoud ALWAYS use PIPETMAN

DIAMOND Tips (in accordance

with ISO 8655) to complete

your pipetting system, because

PIPETMAN DIAMOND Tips were

used to establish the speciﬁcations.

NOTE

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