Gilson Pipetman Manual de usuario

Quick Guide

2QUICK GUIDE |PIPETMAN®

Introduction

PIPETMAN®, recognized as the pipetting standard, is

a fully adjustable, air-displacement pipette that uses

disposable tips. PIPETMAN combines its legendary

accuracy, precision, and durability with easy pipetting

and handling.

PIPETMAN offers a large range of accurate and precise

pipettes with optimized pipetting and purge forces for

hours of pipetting.

Eight single channel models cover a volume range from

0.2 µL to 10 mL.

Eight multichannel models cover a volume range from

1 µL to 300 µL.

Parts Checklist

After unpacking the pipette, verify that the following

items were included and are undamaged:

Single models

●PIPETMAN®

●Quick Guide

●Safety bag

●Certiﬁcate of Conformity (including barcode

sticker)

●Dual-position adapter (for P2 and P10 models

only)

Multichannel models

●PIPETMAN®

●Quick Guide

●Safety bag

●Certiﬁcate of Conformity (including barcode

sticker)

●Ejector spacers for D10 tips (for Px10 models only)

Good Laboratory Practice (GLP) Compliance

The serial number is engraved on the body of the

pipette. It provides unique identification of your pipette

and the manufacturing date.

Ex:

The barcode on the box

and the Certiﬁcate of

Conformity provides

traceability of your pipette.

Figure 1

Serial number and

identification

Year Month Production

number

A A 50001

Serial number

PIPETMAN® |QUICK GUIDEQUICK GUIDE |PIPETMAN®

Specifications

PIPETMAN® is a high-quality pipette that offers

excellent accuracy and precision. The data provided in

the Maximum Permissible Errors table on page 4 was

obtained using PIPETMAN® DIAMOND Tips.

These values are guaranteed only when genuine

PIPETMAN DIAMOND Tips are used.

With a precise pipetting technique the P2 model

may be used to aspirate volumes as low as 0.1 µL

and the P10 model as low as 0.5 µL.

NOTICE

Description

PIPETMAN®

DIAMOND tip

Tip ejector

Tip holder

Color-coded

push button

Blue ring

Thumbwheel

Tip ejector button

Body or

handle

Connecting nut

Figure 2

PIPETMAN® multichannel and single channel model

components

Clip tip ejector

Cover

Ejector spacer

Ejector lock

Ejector support

4QUICK GUIDE |PIPETMAN®

Maximum Permissible Errors

The data in the following table conforms to the

ISO8655-2 standard.

*CV means Coecient of Variation

**P10 model can be used up to 0.5 L with a precise pipetting technique.

***P5000 and P10mL do not have tip ejectors.

PIPETMAN® SINGLE CHANNEL—VARIABLE VOLUME MODELS

Vol.

(µL)

Maximum Permissible Errors

Gilson ISO 8655

Systematic

Error

(µL)

Random

Error

(µL)

Systematic

Error

(%)

Random

Error

(%CV*)

Systematic

Error

(µL)

Random

Error

(µL)

Systematic

Error

(%)

Random

Error

(%CV*)

P2 (P/N F144054M) WITH D10 AND DL10 TIPS

0.2

0.5

± 0.024

± 0.025

± 0.030

≤0.012

≤0.014

± 12.0

± 5.0

± 2.5

± 1.5

≤6.0

≤2.4

≤1.3

≤0.7

± 0.08

≤0.04

± 40.0

± 16.0

± 8.0

± 4.0

≤20.0

≤8.0

≤4.0

≤2.0

P10** (P/N F144055M) WITH D10 AND DL10 TIPS

± 0.025

± 0.075

± 0.100

≤0.012

≤0.030

≤0.040

± 2.5

± 1.5

± 1.0

≤1.2

≤0.6

≤0.4

± 0.12

≤0.08

± 12.0

± 2.4

± 1.2

≤8.0

≤1.6

≤0.8

P20 (P/N F144056M) WITH D200 TIPS

± 0.10

± 0.20

≤0.030

≤0.050

≤0.060

± 5.0

± 1.0

≤1.5

≤0.5

≤0.3

± 0.2

≤0.1

± 10.0

± 2.0

± 1.0

≤5.0

≤1.0

≤0.5

P100 (P/N F144057M) WITH D200 TIPS

100

± 0.35

± 0.40

± 0.80

≤0.10

≤0.12

≤0.15

± 3.5

± 0.8

≤1.0

≤0.24

≤0.15

± 0.8

≤0.3

± 8.0

± 1.6

± 0.8

≤3.0

≤0.6

≤0.3

P200 (P/N F144058M) WITH D200 TIPS

100

200

± 0.50

± 0.80

± 1.60

≤0.20

≤0.25

≤0.30

± 2.5

± 0.8

≤1.0

≤0.25

≤0.15

± 1.6

≤0.6

± 8.0

± 1.6

± 0.8

≤3.0

≤0.6

≤0.3

P1000 (P/N F144059M) WITH D1000 TIPS

100

500

1000

± 3.0

± 4.0

± 8.0

≤0.6

≤1.0

≤1.5

± 3.0

± 0.8

≤0.6

≤0.2

≤0.15

± 8.0

≤3.0

± 8.0

± 1.6

± 0.8

≤3.0

≤0.6

≤0.3

P5000*** (P/N F144066) WITH D5000 TIPS

500

2500

5000

± 12

± 15

± 30

≤3

≤5

≤8

± 2.4

± 0.6

≤0.6

≤0.2

≤0.16

± 40

≤15.0

± 8.0

± 1.6

± 0.8

≤3.0

≤0.6

≤0.3

P10mL*** (P/N F144067) WITH D10mL TIPS

1000

5000

10000

± 30

± 40

± 60

≤6

≤10

≤16

± 3.0

± 0.8

± 0.6

≤0.6

≤0.2

≤0.16

± 60

≤30.0

± 6.0

± 1.2

± 0.6

≤3.0

≤0.6

≤0.3

Gilson maximum permissible errors are only guaranteed when PIPETMAN® pipettes are used

with the recommended PIPETMAN® DIAMOND Tips.

PIPETMAN® |QUICK GUIDEQUICK GUIDE |PIPETMAN®

PIPETMAN® MULTICHANNEL

Vol.

(µL)

Maximum Permissible Errors

Gilson ISO 8655

Systematic

Error

(µL)

Random

Error

(µL)

Systematic

Error

(%)

Random

Error

(%CV*)

Systematic

Error

(µL)

Random

Error

(µL)

Systematic

Error

(%)

Random

Error

(%CV*)

P8X10 (P/N F144068) AND P12X10 (P/N F144069) WITH D10 AND DL10 TIPS

± 0.08

± 0.20

≤0.05

≤0.10

± 8.0

± 4.0

± 2.0

≤5.0

≤2.0

≤1.0

± 0.24

≤0.16

± 24.0

± 4.8

± 2.4

≤16.0

≤.2

≤1.6

P8X20 (P/N F144070) AND P12X20 (P/N F144071) WITH DL10 AND D200 TIPS

± 0.10

± 0.20

± 0.40

≤0.08

≤0.10

≤0.20

± 5.0

± 2.0

≤4.0

≤1.0

± 0.4

≤0.20

± 20.0

± 4.0

± 2.0

≤10.0

≤2.0

≤1.0

P8X200 (P/N F144072) AND P12X200 (P/N F144073) WITH D200 TIPS

100

200

± 0.50

± 1.00

± 2.00

≤0.25

≤0.40

≤0.50

± 2.5

± 1.0

≤1.25

≤0.4

≤0.25

± 3.2

≤1.2

± 16.0

± 3.2

± 1.6

≤6.0

≤1.2

≤0.6

P8X300 (P/N F144074) AND P12X300 (P/N F144075) WITH D300 TIPS

150

300

± 1.00

± 1.50

± 3.00

≤0.35

≤0.60

≤1.00

± 3.33

± 1.0

≤1.17

≤0.4

≤0.33

± 8.0

≤3.0

± 26.67

± 5.33

± 2.67

≤10.0

≤2.0

≤1.0

Setting the Volume

The volume of liquid to be

aspirated is set using the

volume display. The dial colors

are either black or red to

indicate the position of the

decimal point, depending on

the model (refer to Figure 3).

The volume is set by turning

the thumbwheel or the push

button (refer to Figure 4). The

push button makes it easier and

faster to set volumes, especially when wearing gloves.

The thumbwheel may be turned using only one hand to

slowly reach the required setting.

Figure 3

Dial colors by model

1.25 µL

P10

7.5 µL

P20

12.5 µL

P100

75 µL

P200

125 µL

P1000

0.75 mL

P5000

1.25 mL

P10mL

7.5 mL

Px10

7.5 µL

Px20

12.5 µL

Px200

125 µL

Px300

125 µL

To achieve maximum accuracy when setting the volume,

proceed as follows:

●When decreasing the

volume setting, slowly

reach the required setting,

making sure to not

overshoot the mark.

●When increasing the

volume setting, pass the

required value by 1/3 of

a turn and then slowly

decrease to reach the

volume, making sure to

not overshoot the mark.

Figure 4

Location of push button

and thumbwheel

Push button

Thumbwheel

6QUICK GUIDE |PIPETMAN®

Fitting the Tips

Single Channel Models

To fit a new PIPETMAN DIAMOND Tip, push the tip holder

into the tip using a slight twisting motion to ensure a ﬁrm,

airtight seal.

MODEL

COLOR OF VOLUMETER NUMBERS

BLACK RED INCREMENT

P2 µL 0.01 µL 0.002 µL

P10 - P20 - Px10 - Px20 µL 0.1 µL 0.02 µL

P100 - P200 - Px200 - Px300 µL - 0.2 µL

P1000 0.01 mL mL 0.002 mL

P5000 0.01 mL mL 0.002 mL

P10mL mL 0.1 mL 0.02 mL

Figure 5

PIPETMAN® DIAMOND Tip compatibility chart for

single channel models

PIPETMAN DIAMOND Tip

Compatibility for single channel

variable volume models

P2, P10 D10, DL10, DF10ST, DFL10ST

P20 D200, DF30ST

P100 D200, DF100ST

P200 D200, D300, DF200ST, DF300ST

P1000 D1000, D1200, DF1000ST, DF1200ST

P5000 D5000

P10mL D10mL

Fitting the Tips on P2 and P10 Models

NOTE

For the P2 and P10 models, a dual-

position adapter (plastic) is required

to ﬁt DL10 tips (longtips) or D10 tips

(short tips). The metallic rod of the tip

ejector is shaped so the adapter may

be clipped to it in either position.

D5000 and D10mL PIPETMAN

DIAMOND tips do not have any ﬁlter.

P5000 and P10mL models are sold

with a bag of 10 ﬁlters. For more

details, refer to PIPETMAN User’s

Guide LT801122.

NOTICE

PIPETMAN® |QUICK GUIDEQUICK GUIDE |PIPETMAN®

Ejector Spacer for PIPETMAN® 10 µL

Multichannel Models

According to the tip used, D10 or DL10, you may have to

exchange the ejector spacer. The broadly spaced one is

for D10, and the narrowly spaced one is for DL10.

1. Remove the tip ejector, keeping both ejector locks

depressed. Pull the tip ejector down.

2. Gently press the tabs from the ejector spacer and

remove it from the tip

ejector.

3. Insert the alternative

ejector spacer and click

it to the tip ejector.

4. To refit the tip ejector,

gently re-insert the tip

ejector vertically into the

rails of the ejector

support.

P2 and P10 models are delivered with

the adapter in place, ready-to-use

DL10 tips. If D10 tips are used, the

adapter must be repositioned in the

shorter slot as follows:

1. Pull the adapter down from the

metallic rod.

2. Turn the adapter 180°.

3. Reﬁt the adapter so the end of

the metallic rod engages the

shorter slot of the adapter.

NOTE The dual position is

autoclavable.

Figure 6

Dual-position

adapter for P2 and

P10 with stainless

steel tip ejector

Short tips Long tips

Ejector spacer

D10

Short tips

Long tips

Ejector

spacer

Figure 8

Ejector spacer location

PIPETMAN®DIAMOND Tips are best fitted with the

ROCKY RACK technique, invented by Gilson, available

only in our TIPACK and TOWERPACK.

Figure 7

ROCKY RACK

technique

PIPETMAN® DIAMOND Tip Compatibility

for Multichannel Models

P8x10, P12x10 D10*, DL10, DF10, DFL10

P8x20, P12x20 DL10, DFL10, DF30, D200

P8x200, P12x200 D200, D300, DF200, DF300

P8x300, P12x300 D200, D300, DF200, DF300

* Using the broad ejector spacer D10, you can adapt a

D10 tip (see above).

Multichannel models

8QUICK GUIDE |PIPETMAN®

Aspirating

1. Press the push button to the ﬁrst stop

(this corresponds to the set volume of liquid).

2. Hold the pipette vertically and immerse the tip

in the liquid (refer to

PIPETMAN

User’s Guide

LT801122 available on gilson.com)

3. Release the push button slowly and smoothly (to

the top position) to aspirate the set volume of

liquid.

4. Wait one second, and then withdraw the pipette

tip from the liquid. You may wipe any droplets

away from the outside of the tip using a medical

wipe; however, if you do so, take care to avoid

touching the tip’s orifice.

First stop

Second stop

Figure 9

Pipetting motion - aspirate and dispense

Rest position

Pre-Wetting the Tips

Pre-wetting the tips before pipetting helps prepare the

tips for the best pipetting performance. Ideally, the pre-

wet includes both immersing the tip in the liquid and

performing one pipetting step.

Pre-wetting the tips helps ensure that volumes your

pipette’s volume will achieve accuracy and precision

within specifications.

PIPETMAN® |QUICK GUIDEQUICK GUIDE |PIPETMAN®

Maintenance

Routine maintenance will help keep your pipette in

good condition, ensuring a continued high level of

performance.

Maintenance is limited to cleaning or autoclaving

the parts speciﬁed in the

PIPETMAN

User’s Guide

LT801122

, or replacing the push button, connecting nut,

tip ejector, tip holder, seal, and O-ring.

NOTICE

PIPETMAN® P2 and P10 should not be

disassembled. You may only replace the

push button, tip ejector, and its dual-position

adapter. With these pipettes, if the tip holder

is damaged, the piston may also be damaged.

After replacing any parts, you should verify

the performance of your pipette following the

veriﬁcation procedure available on the Gilson

website (www.gilson.com). If the pipette needs

to be readjusted, please contact your local

authorized Gilson Service Center.

Dispensing

1. Place the end of the tip against the inside wall of the

recipient vessel (at an angle of 10° to 40°).

2. Press the push button slowly and smoothly to the

first stop.

3. Wait for at least one second, then press the push

button to the second stop to expel any residual

liquid from the tip. Keep the push button pressed

fully down and (while removing the pipette) draw

the tip along the inside surface of the vessel.

4. Release the push button smoothly. Eject the tip by

pressing firmly on the tip ejector button.

It is recommended to regularly verify the volume

displayed on the dial while pipetting.

NOTICE

Decreased pipetting forces are due to the

design of the piston assembly, which includes

a very high-quality lubricant (part number

5440011070). The use of any other lubricant

voids the warranty of this pipette.

NOTICE

Gilson recommends maintenance and

calibration at least annually, more frequently as

needed, depending on use.

Cleaning and Decontamination

PIPETMAN is designed so the parts normally in contact

with liquid contaminants can easily be cleaned and

decontaminated.

For further information, please refer to PIPETMAN User’s

Guide LT801122 available on gilson.com.

10 QUICK GUIDE |PIPETMAN®

Warranty

Gilson warrants this pipette against defects in material

under normal use and service for a period of three years

for both single channel and multichannel models from

the date of purchase.

This warranty shall not apply to pipettes which are

subject to abnormal use and/or improper or inadequate

maintenance (contrary to the recommendations given in

the user’s guide), including, but not limited to pipettes

that have been subjected to physical damage, improper

handling, or spillage or exposure to any corrosive

environment. This warranty shall also be void in the

event pipettes are altered or modified by any party

other than Gilson or its designates. Gilson’s sole liability

under this warranty shall be limited to, at Gilson’s

sole option, repair or replacement of any defective

components of pipettes or refund of the purchase price

paid for such pipettes. Routine cleaning, control and

recalibration are not covered under the warranty. The

replacement of wearing parts such as seals, O-rings,

broken pistons assembly, and broken tip holders are

not covered under the warranty.

NOTICE

Yearly routine maintenance is highly

recommended to keep your pipette in good

condition, ensuring a continued high level of

performance.

THE FOREGOING WARRANTY IS EXCLUSIVE

AND GILSON HEREBY DISCLAIMS ALL OTHER

WARRANTIES, WHETHER EXPRESS OR

IMPLIED, INCLUDING ANY WARRANTIES OF

MERCHANTABILITY AND ANY WARRANTIES OF

FITNESS FOR A PARTICULAR PURPOSE, UNDER

NO CIRCUMSTANCES SHALL GILSON BE LIABLE

FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT

OR INCIDENTAL DAMAGES ARISING OUT OF ANY

BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.

Web Resources

Visit Gilson’s website at www.gilson.com for:

●Service contact information

●PIPETMAN® User’s Guide LT801122 pdf

●Information about accessories, spare parts, and

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