Agilent Technologies G1314B VWD Manual de usuario

A

Agilent 1200 Series

Variable Wavelength

Detector

G1314B /G1314C (SL)

User Manual

2 1200 Series Variable Wavelength Detector User Manual

Notices

No part of this manual may be reproduced in

any form or by any means (including

electronic storage and retrieval or

translation into a foreign language) without

prior agreement and written consent from

Agilent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G1314-90010

Edition

02/06

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn, Germany

Manual Structure

The User Manual G1314-90010 (English)

and its localized versions contain a subset

of the Service Manual and is shipped with

the detector in printed matter.

Latest versions of the manuals can be

obtained from the Agilent web.

The Service Manual G1314-90110 (English)

contains the complete information about

the Agilent 1200 Series Variable

Wavelength Detector. It is available as

Adobe Reader file (PDF) only.

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connec-

tion with the furnishing, use, or per-

formance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a license

and may be used or copied only in

accordance with the terms of such license.

Restricted Rights Legend

Software and technical data rights granted

to federal government customers include

only those rights customarily provided to

end user Customers of Software. Agilent

provides this customary commercial license

in Software and technical data pursuant to

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(Computer Software) and, for Department of

Defense purchases, DFARS 252.227-7015

(Technical Data - Commercial Items) and

DFARS 227.7202-3 (Rights in Commercial

Computer Software or Computer Software

Documentation). If a federal government or

other public sector Customer has a need for

rights not conveyed under these terms, it

must negotiate with Agilent to establish

acceptable terms in a written agreement

executed by all relevant parties.

Safety Notices

CAUTION

A CAUTION notice denotes a haz-

ard. It calls attention to an operat-

ing procedure, practice, or the like

that, if not correctly performed or

adhered to, could result in damage

to the product or loss of important

data. Do not proceed beyond a

CAUTION notice until the indicated

conditions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in personal injury or death. Do not

proceed beyond a WARNING

notice until the indicated condi-

tions are fully understood and

met.

1200 Series Variable Wavelength Detector User Manual 3

In This Manual…

This manual covers the Agilent 1200 Series Variable Wavelength

Detectors

1 Introduction to the Variable Wavelength Detector

This chapter gives an introduction to the detector, instrument

overview and internal connectors.

2 Site Requirements and Specifications

This chapter gives information on environmental requirements,

physical and performance specifications.

3 Installing the Detector

This chapter describes the installation of the detector.

4 Using the Detector

This chapter provides information on how to set up the detector

for an analysis and explains the basic settings.

5 How to optimize the detector

This chapter gives hints on how to select the detector

parameters and the flow cell.

7 Maintenance and Repair

This chapter provides general information on maintenance and

repair of the detector.

8 Maintenance

This chapter describes the maintenance of the detector.

G1314B Agilent 1200 Series VWD

G1314C Agilent 1200 Series VWD-SL

4 1200 Series Variable Wavelength Detector User Manual

9 Parts and Materials for Maintenance

This chapter provides information on parts for maintenance.

A Appendix

This chapter provides addition information on safety, legal and

web.

1200 Series Variable Wavelength Detector User Manual 5

Contents

1 Introduction to the Variable Wavelength Detector

Introduction to the Detector 10

Optical System Overview 11

Electrical Connections 15

Instrument Layout 17

Early Maintenance Feedback (EMF) 18

EMF Counter 18

Using the EMF Counters 18

2 Site Requirements and Specifications

Site Requirements 22

Physical Specifications 24

Performance Specifications 25

3 Installing the Detector

Unpacking the Detector 28

Optimizing the Stack Configuration 30

Installing the Detector 33

Flow Connections to the Detector 36

4 Using the Detector

Setting up an Analysis 40

Before Using the System 40

Requirements and Conditions 42

6 1200 Series Variable Wavelength Detector User Manual

Optimization of the System 44

Preparing the HPLC System 45

Running the Sample and Verifying the Results 54

Special Settings of the Detector 55

Control Settings 55

Online Spectra 56

Scanning with the VWD 57

Analog Output Settings 59

Special Setpoints 60

Peakwidth Settings 61

Optimizing the Detector 63

5 How to optimize the detector

Optimizing the Detector Performance 66

Match the Flow Cell to the Column 66

Set the Detector Parameters 69

6 Troubleshooting and Diagnostics

Overview of the Detector’s Indicators and Test Functions 72

Status Indicators 73

Power Supply Indicator 73

Detector Status Indicator 74

User Interfaces 75

Agilent LC Diagnostic Software 76

7 Maintenance and Repair

Introduction to Maintenance and Repair 78

Simple Repairs - Maintenance 78

Exchanging Internal Parts - Repair 78

Warnings and Cautions 79

1200 Series Variable Wavelength Detector User Manual 7

Cleaning the Detector 80

Using the ESD Strap 81

8 Maintenance

Overview of Maintenance 84

Exchanging a Lamp 85

Exchanging a Flow Cell 87

Repairing the Flow Cells 90

Using the Cuvette Holder 94

Correcting Leaks 97

Replacing Leak Handling System Parts 98

Replacing the Interface Board 99

Replacing the Detector’s Firmware 100

Tests & Calibrations 101

Intensity Test 102

Wavelength Verification/Calibration 104

Holmium Oxide Test 105

9 Parts and Materials for Maintenance

Overview of Maintenance Parts 110

Standard Flow Cell 111

Micro Flow Cell 112

Semi-micro Flow Cell 113

High Pressure Flow Cell 115

Cuvette Holder 116

Leak Parts 117

8 1200 Series Variable Wavelength Detector User Manual

Accessory Kit 118

A Appendix

General Safety Information 120

Lithium Batteries Information 123

Radio Interference 124

Sound Emission 125

UV-Radiation 126

Solvent Information 127

Declaration of Conformity for HOX2 Filter 129

Agilent Technologies on Internet 130

Index

Agilent 1200 Series Variable Wavelength Detector

User Manual

Agilent Technologies

Introduction to the Variable Wavelength

Detector

Introduction to the Detector 10

Optical System Overview 11

Flow Cell 12

Electrical Connections 15

Instrument Layout 17

Early Maintenance Feedback (EMF) 18

This chapter gives an introduction to the detector, instrument overview and

internal connectors.

10 1200 Series Variable Wavelength Detector User Manual

1 Introduction to the Variable Wavelength Detector

Introduction to the Detector

The Agilent 1200 Series variable wavelength detector is designed for highest

optical performance, GLP compliance and easy maintenance with:

•higher data rate (27/55Hz) for fast-HPLC with G1314C VWD-SL, see “Set the

Detector Parameters" on page 69,

•deuterium lamp for highest intensity and lowest detection limit over a

wavelength range of 190 to 600 nm,

•optional flow-cell cartridges (standard 10 mm 14 µl, high pressure 10 mm

14µl,micro5mm1µl,semi-micro6mm5µl)areavailableandcanbeused

depending on the application needs,

•easy front access to lamp and flow cell for fast replacement, and

•built-in holmium oxide filter for fast wavelength accuracy verification.

For specifications “Performance Specifications" on page 25.

Two version of the Agilent 1200 Series variable wavelength detector are

available:

G1314B VWD 1200 Series Variable Wavelength Detector

standard version

G1314C VWD-SL 1200 Series Variable Wavelength Detector SL

high data rates for fast HPLC

NOTE The G1314C VWD-SL can be operated with a G1323B Control Module just in standard mode

as G1314B - no higher data rate selection is available.

Este manual sirve para los siguientes modelos

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