Agilent Technologies InfinityLab 1290 Infinity II Manual de usuario

Agilent InfinityLab

Evaporative Light Scattering Detectors

User Manual

Agilent InfinityLab ELSD User Manual

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Document No: D0013647 Rev. A

Edition: 08/2022

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Agilent InfinityLab ELSD User Manual 3

In This Book

This manual contains information on:

•Agilent 1260 Infinity II ELSD (G4260B)

•Agilent 1290 Infinity II ELSD (G7102A)

1 Introduction to the Detector

This chapter gives an introduction to the module, instrument overview, and internal

connectors.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical, and

performance specifications.

3 LAN Configuration

This chapter provides information on connecting the module to the control

software.

4UsingtheModule

This chapter explains the operational parameters of the ELSD.

5 Optimizing Performance

This chapter gives hints on how to optimize the performance or use additional

devices.

6Troubleshootingand Diagnostics

This chapter gives an overview about the troubleshooting and diagnostic features.

7 Error Information

This chapter describes the meaning of error messages, and provides information

on probable causes and suggested actions on how to recover from error

conditions.

Agilent InfinityLab ELSD User Manual 4

8 Maintenance

This chapter describes the maintenance of the ELSD.

9 Parts and Materials for Maintenance

This chapter provides information on parts for maintenance.

10 Appendix

This chapter provides addition information on safety, legal, and Web.

Agilent InfinityLab ELSD User Manual 5

Contents

1 Introduction to the Detector 7

Introduction to the ELSD 8

System Overview 9

2 Site Requirements and Specifications 16

Pre-installation Requirements 17

Site Requirements 18

Physical Specifications 21

Performance Specifications 23

3 LAN Configuration 27

What You Have to Do First 28

TCP/IP Parameter Configuration 29

PC and OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom

Software Setup 36

4UsingtheModule41

Before Using the Detector 42

Instrument Controls 43

Operational Parameters 48

Controlling the ELSD During an Injection 52

Controlling the ELSD within OpenLab CDS, OpenLab ChemStation or

OpenLab EZChrom 54

General Considerations 55

5 Optimizing Performance 59

Do’s and Don’ts of ELS Detection 60

Location of the Detector Module 61

Pumping Systems 62

Mobile Phase Priming 63

Agilent InfinityLab ELSD User Manual 6

6 Troubleshooting and Diagnostics 64

Troubleshooting 65

Troubleshooting an HPLC System 66

General Problems 67

7 Error Information 74

What Are Error Messages 75

Module Specific Error Messages 76

8 Maintenance 83

Introduction to Maintenance 84

Cautions and Warnings 85

Cleaning the Module 87

Inspection of Cables 88

Drying the Diffuser 89

Cleaning the Nebulizer 90

Cleaning Evaporator Tube 91

Putting the Instrument into Storage 92

Updating Detector Firmware 93

9 Parts and Materials for Maintenance 98

Identifying Parts and Materials 99

10 Appendix 100

General Safety Information 101

Waste Electrical and Electronic Equipment (WEEE) Directive 105

Radio Interference 106

Agilent Technologies on Internet 107

Agilent InfinityLab ELSD User Manual 7

1 Introduction to the Detector

Introduction to the ELSD 8

System Overview 9

Basic Principles of Operation 9

Operational Parameters 12

Overview of ELS Detector 13

This chapter gives an introduction to the module, instrument overview, and

internal connectors.

1 Introduction to the Detector

Introduction to the ELSD

Agilent InfinityLab ELSD User Manual 8

Introduction to the ELSD

The Evaporative Light Scattering Detector is a unique and highly sensitive

detector for semi-volatile and non-volatile solutes in a liquid stream. It is mainly

used as a concentration detector for High Performance Liquid Chromatography

(HPLC). The solvent stream containing the solute material is nebulized and

carried by a gas flow through an evaporation chamber. The solvent is volatilized,

leaving a mist of solute particles that scatter light to a photosensitive device. The

signal is amplified and a voltage output provides the concentration of the solute

particles passing through the light.

The Agilent 1260 Infinity II ELSD or Agilent 1290 Infinity II ELSD may be used

alone, or as one of several detectors in a GPC or HPLC system. As the solvent or

eluent is evaporated during the analysis, the Agilent 1260 Infinity II ELSD or

Agilent 1290 Infinity II ELSD must be the last in series if used with other

detectors. If the Agilent 1260 Infinity II ELSD or Agilent 1290 Infinity II ELSD is

being used as the last detector in a series, care must be taken not to exceed the

recommended backpressure in detector cells in other units.

This manual instructs the user in the installation and operation of the Agilent

1260 Infinity II ELSD or Agilent 1290 Infinity II ELSD for standalone use and

control using Agilent OpenLab CDS, OpenLab ChemStation, or OpenLab

EZChrom.

Agilent InfinityLab ELSD User Manual 9

1 Introduction to the Detector

System Overview

Basic Principles of Operation

Nebulization

The eluent inlet is connected to the nebulizer via a short length of stainless steel

capillary tube. The incoming eluent stream passes through the heated nebulizer

and is mixed with the incoming nebulizer gas stream. The mixed gas and eluent

stream form an aerosol plume containing a uniform dispersion of droplets that

then passes as a continuous flow into the evaporator section. Any larger droplets

or the inefficiently nebulized droplets collect in the nebulizer chamber waste trap

and then drain off via the waste outlet into a collection bottle.

Evaporation

After nebulization, the atomized spray is propelled through the evaporation tube

assisted by the carrier gas. In the evaporator section, the solvent is removed

leaving a stream of dry particles of the analyte. A diffuser located in the

evaporator assists in the drying of the particles, acting as an efficient heat

exchanger, prevents ballistic particles reaching the scattering chamber and

randomizes the particle plume. The ELS Detector uses patented gas flow

technology in the evaporation zone to aid evaporation at low temperatures. By

adding a stream of dry nitrogen (evaporation gas) at the entrance of the

evaporator tube less volatile solvents (for example, water) are easily evaporated.

This evaporation gas is controlled by the user and facilitates subambient

operation.

1 Introduction to the Detector

System Overview

Agilent InfinityLab ELSD User Manual 10

Detection

Light in the optical chamber is passed through the instrument at right angles to

the direction of particle flow. A light trap is located opposite the source of light to

capture the transmitted incident beam eliminating internal reflections within the

instrument body. When pure solvent is being evaporated, only its vapor passes

through the light path and the amount of light scattered to the photomultiplier is

small and gives a constant baseline response. When a nonvolatile solute is

present a particle cloud passes through the light path, causing light to be

scattered. This scattered light enters the optical aperture of the detection system

and generates a signal response from the photodiode in real time. The quantity

of light detected depends on the solute concentration and solute particle size

distribution.

Figure 1 Principles of Operation

1. Nebulization 2. Evaporation 3. Detection

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