Agilent Technologies G1978B Manual de usuario

Agilent Technologies

Agilent G1978B

Multimode Source for

6410 Triple Quad LC/MS

User Guide

2 Multimode Source for 6410 Triple Quad LC/MS User Guide

Notices

No part of this manual may be reproduced in

any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G1978-90010

Edition

First Edition, October 2006

Printed in USA

Agilent Technologies, Inc.

5301 Stevens Creek Blvd.

Santa Clara, CA 95051 USA

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connec-

tion with the furnishing, use, or per-

formance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a license

and may be used or copied only in accor-

dance with the terms of such license.

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ware and technical data rights granted to

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252.227-7015 (Technical Data - Commercial

Items) and DFARS 227.7202-3 (Rights in

Commercial Computer Software or Com-

puter Software Documentation).

Safety Notices

CAUTION

A CAUTION notice denotes a haz-

ard. It calls attention to an operat-

ing procedure, practice, or the like

that, if not correctly performed or

adhered to, could result in damage

to the product or loss of important

data. Do not proceed beyond a

CAUTION notice until the indicated

conditions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in personal injury or death. Do not

proceed beyond a WARNING

notice until the indicated condi-

tions are fully understood and

met.

Windows®and MS Windows®are U.S.

registered trademarks of Microsoft Corpora-

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Windows NT®is a U.S. registered trade-

mark of Microsoft Corporation.

Multimode Source for 6410 Triple Quad LC/MS User Guide 3

In This Guide

This guide explains how to install, maintain and troubleshoot your multimode

ion source.

1Basic Operation and Maintenance

This chapter describes basic operation and maintenance for the multimode

source.

2Installation

This chapter tells you how to install the multimode source.

3Reference

This chapter contains an overview of the multimode source, safety

precautions, and technical specifications.

4 Multimode Source for 6410 Triple Quad LC/MS User Guide

Contents

Multimode Source for 6410 Triple Quad LC/MS User Guide 5

Content

1 Basic Operation and Maintenance 7

To set up a method to use the multimode source 8

To open the multimode source 10

To clean the multimode source daily 11

To clean the multimode source weekly 13

To check tuning with the multimode source 15

2 Installation 17

Step 1. Prepare to install 18

Step 2. Install the HV control PCA and cables 19

To remove the multimode source 23

To convert from multimode to ESI or APCI 24

To convert from ESI or APCI to the multimode source 25

3 Reference 31

Safety and Specifications 32

Safety 32

Safety Symbols 36

Technical Specifications 37

Overview 38

Benefits of a multimode source 38

Mobile phase considerations when using the multimode source 46

Source comparison in positive ion mode 48

Source comparison in negative ion mode 49

Guidelines 50

Guidelines for multimode source settings in MM-ES mode 50

Guidelines for multimode source settings in MM-APCI mode 51

Guidelines for multimode source settings in MM-ES+APCI mode 52

Empirical Formulas for high resolution MS 53

6 Multimode Source for 6410 Triple Quad LC/MS User Guide

Contents

G2421A MM-ES Calibration Ions 53

G2432A MM-APCI Calibration Ions 54

Tuning Mix and Test Mix 55

G2421A ES Tuning Mix (100mL) 55

G2432A APCI Tuning Mix (100mL) 56

Chemical structures in the multimode test mix 57

Agilent G1978B Multimode Source for 6410 Triple Quad LC/MS

User Guide

Agilent Technologies

Basic Operation and Maintenance

To set up a method to use the multimode source 8

To open the multimode source 10

To clean the multimode source daily 11

To clean the multimode source weekly 13

To check tuning with the multimode source 15

This chapter describes the tasks that you need to operate and maintain the

multimode source.

8 Multimode Source for 6410 Triple Quad LC/MS User Guide

1 Basic Operation and Maintenance

To set up a method to use the multimode source

1In the MassHunter software, change the Context to Acquisition.

2In the MS QQQ tab, set Ion source to MMI (see Figure 1 on page 9).

3In the Time Segments table, chose an ionization mode from the Ion Mode

list. You may set the ionization mode to one of the following:

•ESI

•APCI

•ESI+APCI

The Ion Mode selection ESI + APCI will specify a method for alternating ESI

and APCI operation.

Note that the Ion Mode selection is only visible if Ion source is set to MMI.

4In the Source tab, set the desired source conditions. See the

“Guidelines” on page 50 for suggested source conditions for the multimode

source for the different ionization modes.

5Make any other changes that are necessary for your method.

6Save the method.

Multimode Source for 6410 Triple Quad LC/MS User Guide 9

Basic Operation and Maintenance 1

To set up a method to use the multimode source

Figure 1 Multimode acquisition settings

WARNING The LC/MS diverter valve is an integral part of the G1978B safety system. The LC

mobile phase flow must always be connected to the diverter valve inlet filter. Never

bypass the diverter valve and connect directly to the nebulizer. If the diverter valve is

used in a manner not specified by Agilent Technologies, the protections provided by

the diverter valve may be impaired and the system may catch fire.

10 Multimode Source for 6410 Triple Quad LC/MS User Guide

1 Basic Operation and Maintenance

To open the multimode source

Open the multimode source to access the end cap and the capillary cap for

cleaning and inspection.

1Turn off the multimode source temperatures and flows:

aChange the Context view to Acquisition.

bClick the MS QQQ tab.

cTurn off all voltages and temperatures in the Source tab.

dWait approximately 20 minutes for the source to cool down.

2Open the spray chamber cover by pulling the latch.

The high voltage automatically turns off when the chamber door is opened

so that no high voltages are present within the chamber.

3Check that the vaporizer temperature sensor is straight and extends 15mm

from back of chamber.

4Check that the separator is aligned vertically.

5Check that the APCI corona needle is in and extends approximately 3mm

from the corona guide.

6Check that the source is clean.

WARNING Do not touch the multimode source or the capillary cap. They may be very hot. Let the

parts cool before you handle them.

WARNING Never touch the source surfaces, especially when you analyze toxic substances or

when you use toxic solvents. The source has several sharp pieces which can pierce

your skin including the APCI corona needle, vaporizer sensor and counter current

electrode.

WARNING Do not insert fingers or tools through the openings on the multimode chamber. When

in use, the capillary and capillary cap are at high voltages up to 4 kV.

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