Agilent Technologies 1200 series Manual de usuario

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Agilent 1200 Series

Standard and

Preparative

Autosamplers

Reference Manual

2 Agilent 1200 Series Autosampler Reference Manual

Notices

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Manual Part Number

G1329-90010

Edition

Edition 02/06

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Agilent 1200 Series Autosampler Reference Manual 3

In This Guide…

1 Installing the Autosampler

Site requirements and installation of the autosampler

2 Optimizing Performance

Autosamplers are more and more used in HPLC to improve the

productivity in the laboratories and the consistency and

accuracy of analytical results

This chapter shows how to optimize the autosamplers to

achieve best results

3 Troubleshooting and Test Functions

The modules built-in troubleshooting and test functions

4RepairingtheAutosampler

Instructions on simple, routine repair procedures as well as

more extensive repairs requiring exchange of internal parts

5 Parts and Materials

Detailed illustrations and lists for identification of parts and

materials

6Introduction to the Autosampler

An introduction to the standard and preparative autosamplers

Theory of Operation

Theory of operation of mechanical hardware, electronics, and

instrument interfaces

8 Introduction to the Control Module

Introduction to the screens available for operation of the

Agilent 1200 Series autosamplers with the control module

4 Agilent 1200 Series Autosampler Reference Manual

9 Specifications

Performance specifications of the Agilent 1200 Series Standard

and Preparative Autosamplers

Agilent 1200 Series Autosampler Reference Manual 5

Contents

1 Installing the Autosampler 13

Site Requirements 14

Power Consideration 14

Power Cords 15

Bench Space 15

Environment 16

Unpacking the Autosampler 18

Optimizing the Stack Configuration 21

Installing the Autosampler 26

Installing the Thermostatted Autosampler 29

Flow Connections 34

Sample Trays 36

Choice of Vials and Caps 39

Transporting the Autosampler 42

2 Optimizing Performance 43

Optimization for Lowest Carry-over 44

Using the Automated Needle Wash 45

Using an Injector Program 46

General Recommendation to Lowest Carry-over 47

Fast Injection Cycle and Low Delay Volume 48

Overlapped Injection Mode 48

General Recommendations for Fast Injection Cycle Times 49

Precise Injection Volume 50

6 Agilent 1200 Series Autosampler Reference Manual

Contents

Draw and Eject Speed 50

Choice of Rotor Seal 52

3 Troubleshooting and Test Functions 53

Overview of the Sampler’s Indicators and Test Functions 54

Status Indicators 55

Power Supply Indicator 55

Instrument Status Indicator 56

Error Messages 57

Timeout 58

Shutdown 59

Remote Timeout 60

Synchronization Lost 61

Leak 62

Leak Sensor Open 63

Leak Sensor Short 64

Compensation Sensor Open 65

Compensation Sensor Short 66

Fan Failed 67

Open Cover 68

Restart Without Cover 69

Arm Movement Failed 70

Valve to Bypass Failed 71

Valve to Mainpass Failed 72

Needle Up Failed 73

Needle Down Failed 74

Missing Vial 75

Initialization Failed 76

Metering Home Failed 77

Motor Temperature 78

Initialization with Vial 79

Safety Flap Missing 80

Contents

Agilent 1200 Series Autosampler Reference Manual 7

Vial in Gripper 81

Missing Wash Vial 82

Invalid Vial Position 83

Maintenance Functions 84

User Interface 85

Change Needle 86

Change Piston 88

Park Arm (Park Gripper) 89

Change Gripper (Change Arm) 90

Tray Alignment 91

Step Commands 93

Troubleshooting 95

Troubleshooting Guide for the Sample Transport Assembly 97

Intermittent lock-ups with or without vial in the gripper fingers 98

Jittery (shaky) movement in X and or theta axes and/or when the needle goes

through the gripper arm into the via 100

Poor alignment, seen during vial pickupand vial replacement and/or when the

needle hits the gripper arm 102

4RepairingtheAutosampler105

Introduction into Repairing the Autosampler 106

Using the ESD Strap 107

Overview of Main Repair Procedures 109

Simple Repairs 110

Needle Assembly 111

Needle-Seat Assembly 114

Stator Face 117

Rotor Seal 120

Metering Seal and Plunger 124

Gripper Arm 128

8 Agilent 1200 Series Autosampler Reference Manual

Contents

Interface Board 131

Exchanging Internal Parts 132

Assembling the Main Cover 133

Top Cover and Foam 134

Installing the Top Cover and Foam 136

Illumination Assembly 138

Transport Assembly 139

Installing the Transport Assembly 140

Sampling Unit 141

Installing the Sampling Unit 144

Injection-Valve Assembly 147

Metering-Drive Motor and Belt 150

Needle-Drive Motor and Belt 152

Fan 155

ASM Board 157

Changing the Product Number or Serial Number 160

Using the Agilent ChemStation 160

Using the Instant Pilot G4208A 161

Using the Control Module G1323B 162

SUD Board 167

Power Supply 169

Leak Sensor 172

5 Parts and Materials 175

Main Assemblies 176

Sampling Unit Assembly 178

Analytical-Head Assembly 181

Injection-Valve Assembly 184

Sheet Metal Kit 186

Cover Parts 187

Contents

Agilent 1200 Series Autosampler Reference Manual 9

Foam Parts 188

Power and Status Light Pipes 189

Leak System Parts 190

Vial Trays 191

Standard Autosampler Accessory Kit G1329-68725 192

Preparative Autosampler Accessory Kit G2260-68705 193

Maintenance Kit G1313-68709 194

Multi-Draw Kit G1313-68711 195

900 µl Injection Upgrade Kit G1363A for G1329A 196

External Tray G1313-60004 197

6 Identifying Cables 199

Cable Overview 200

Analog Cables 202

Remote Cables 205

BCD Cables 210

Auxiliary Cable 212

CAN Cable 213

External Contact Cable 214

RS-232 Cable Kit 215

LAN Cables 216

7 Introduction to the Autosampler 217

Introduction to the Autosampler 218

Sampling Sequence 220

Injection Sequence 221

Sampling Unit 223

Needle-Drive 224

Analytical head / preparative head 224

10 Agilent 1200 Series Autosampler Reference Manual

Contents

Injection-Valve 225

Transport Assembly 226

Early Maintenance Feedback (EMF) 228

EMF Counters 228

Using the EMF Counters 229

Electrical Connections 230

8 Theory of Operation 233

Autosampler Control and Electronics 234

Position and Movement Sensors 235

Autosampler Main Board (ASM) 236

Firmware Description 241

Firmware Updates 242

Optional Interface Boards 243

BCD Board 243

LAN Communication Interface Board 245

Interfaces 247

Analog Signal Output 248

GPIB Interface 248

CAN Interface 248

Remote Interface 248

RS-232C 250

Setting the 8-bit Configuration Switch 252

GPIB Default Addresses 253

Communication Settings for RS-232C Communication 254

Forced Cold Start Settings 255

Stay-Resident Settings 256

The Main Power Supply Assembly 257

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