Agilent Technologies 1260 Infinity II Manual de usuario

Agilent Technologies

Agilent 1260 Infinity II

High Temperature

Sample Preparation

System

User Manual

Revision 10.0 November 2016

Notices

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States and international copyright laws.

Manual Information

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Agilent Technologies

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(November 1995), as applicable in any

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Safety Notices

A CAUTION notice denotes a hazard. It calls

attention to an operating procedure, practice,

or the like that, if not correctly performed or

adhered to, could result in damage to the

product or loss of important data. Do not

proceed beyond a CAUTION notice until the

indicated conditions are fully understood and

met.

A WARNING notice de notes a hazard. It

calls attention to an operating procedure,

practice, or the like that, if not correctly

performed or adhered to, could result in

personal injury or death. Do not proceed

beyond a WARNING notice until the

indicated conditions are fully understood

and met.

Health and Safety

Agilent 1260 Infinity II High Temperature Sample Preparation System

The use of this instrument does not entail any hazard if used in accordance with the instructions

stated within the Operator’s manual. The protection provided by this equipment may be impaired if

it is used with accessories not provided or recommended by Agilent or used in a manner not

specified in this manual.

Users should observe the following safety precautions:

Ensure that all the instructions within the Operator’s manual are understood and carried out in the

operation of the instrument. All persons utilising the instrument should have adequate training in

its proper set-up, operation, and particularly its safety features.

Voltages above 210V AC are present within the instrument, and access covers should not be

removed by anyone other than properly trained service personnel. No attempt should be made to

service the instrument without authorisation from Agilent’s service department and contravention

of this may result in personal hazard or damage to the instrument and will invalidate the

manufacturer's warranty.

We stress the importance of standard safe practice (such as COSHH regulations) for dealing with

electronic laboratory equipment, solvents, etc., in preventing accidents, fires, or potentially

hazardous conditions.

The individual or group responsible for the use and maintenance of this equipment must ensure

that appropriate decontamination is carried out if hazardous material is spilt on or inside the

instrument.

Before using any cleaning or decontamination method except those recommended by Agilent, Inc.,

users should check with Agilent that the proposed method will not damage the equipment. See

Section 6 for detailed recommended procedures.

If in any doubt about the use of the instrument contact your local Agilent office or local distributor.

Contents

NOTICES II

HEALTH AND SAFETY III

1GENERAL INFORMATION 1

1.1 Overview 1

1.2 Specifications 1

1.3 Unpacking 2

1.4 Location 2

1.5 Utility Requirements 3

1.6 Safety Precautions 3

2SYSTEM OVERVIEW 5

3INSTALLING THE MODULE 8

3.1 Preparing the system 8

4SYSTEM OPERATION 9

4.1 Power On 9

4.2 Shaker 9

4.3 Temperature Controller 12

4.4 Safety Over-Temperature Cut-Out 13

4.5 Hand Pipettor 14

5SAMPLE PREPARATION 15

5.1 Procedure 15

5.2 Cleaning the Frits 16

6CLEANING AND DECONTAMINATION PROCEDURE 17

7MAINTENANCE 18

SAFETY STANDARDS 20

ABSTRACT 21

Agilent 1260 HT Sample Prep System Operation Manual

1General Information

1.1 Overview

Agilent’s high temperature sample preparation system is designed for routine dissolution and filtration

of polymer solutions at temperatures up to 260 °C. Controlled heating and careful shaking of the

samples assists dissolution, and a unique hand pipettor device, fitted with an interchangeable frit

magazine, efficiently filters and dispenses the hot solution directly into autosampler vials with minimal

handling. The vials then can be crimped and transferred directly into the high temperature carousel.

1.2 Specifications

Type

Specification

Temperature Range

Ambient to 260 °C

Temperature Accuracy

±2 °C

Temperature Control

PI Control with RTD temperature sensor

Number of Samples

Capacity of preparation vial

minm2 mLs maxm20 mLs

Mode of sample agitation

Horizontal motion controlled via on/off switch with variable speed control

Sample transfer

Hand held pipettor

Filtration

0.5, 2, 5, 10 µm stainless steel frit or 1 µm glass fibre disk

Safety

Overvoltage category II

Pollution degree 2

Max. operating altitude 2000 m

Indoor use only

Independent over-temperature cut-out

Power Requirements:

210-250V AC, line frequency 50/60Hz

(max. voltage fluctuation ± 10 %).

See rating plate on instrument.

Environmental Conditions:

10 °C –30 °C,

< 95% rH, non-condensing

Dimensions:

Packaged size

505 x 585 x 205 mm (w x d x h)

1000 x 600 x 500 mm (w x d x h)

Weight:

Packaged weight

40kg, 88lbs.

45kg, 99lbs

Agilent 1260 HT Sample Prep System Operation Manual

1.3 Unpacking

Care has been taken to ensure that the instrument should be received in proper condition. The

packing and protection are designed for normal hazards of road, rail or air transit. Any damages to

the container or instrument should be reported immediately to your local distributor or to Agilent,

Inc. It is recommended that the shipping container be kept, if possible, for re-shipment or return to a

service centre.

Examine the shipping box for visible signs of exterior damage. Unpack the instrument and examine

for transit damage. Refer to local manual lifting guidelines and regulations. It is recommended that

instrument be lifted by a minimum of two people. Check that all items on the packing list are

included.

Notify your local distributor or Agilent of any damage or missing items.

This instrument is heavy (40kg)

1.3.1 Packing list

Sample Preparation System

Hand pipettor

10 x Filter magazines fitted with 2 µm frits

1 pack of sample preparation vials (20 mL, 100)

1 pack of aluminium crimp caps (100)

2” Exhaust ducting (2M)

2” Jubilee clip

4 mm nylon tubing for gas purge (2M)

1.4 Location

The Sample Preparation System must be supported by a sturdy, level bench. Preferably

avoid exposure to direct sunlight.

The unit must be sited in a fume cupboard or suitably extracted environment when used with

hazardous and dangerous chemicals.

Provide adequate space around the unit to allow for the motion of the horizontal shaker.

Agilent 1260 HT Sample Prep System Operation Manual

Ensure that the power switch & appliance power connector situated at the rear of the unit

remain easily accessible at all times.

Do not use this equipment in hazardous atmospheres or with hazardous materials for which it

was not designed.

1.5 Utility Requirements

1.5.1 Electrical Power

Single phase mains power is required. The instrument is designed for connection to a Cat II

installation.

Before plugging in the power cable, ensure the instrument voltage rating matches your local

power supply.

Use only a supply with protective grounding.

The correct fuses should be fitted:

230V (AC): 2x 250V 5A F HBC 20 mm fuses (inlet)

1x 250V 5A FF HBC (heater).

RISK OF FIRE, REPLACE FUSES AS MARKED!

THE UNIT IS DOUBLE-FUSED. DISCONNECT POWER CORD BEFORE

REMOVING COVERS!

1.5.2 Extraction

The unit should be positioned in a fume hood or extracted area. Additional extraction via a 2” duct

may also be required

1.5.3 Nitrogen Supply

The hood of the unit is fitted with a 4 mm push-fit gas pipe coupling. Nitrogen purge gas should be

fed into the hood at approx 1SLM.

1.6 Safety Precautions

1.6.1 Condensation within the Module

Condensation will damage the system electronics.

➔

Do not store, ship or use your module under conditions where temperature

fluctuations could cause condensation within the module.

➔

If your module was shipped in cold weather, leave it in its box and allow it to

warm slowly to room temperature to avoid condensation.

Agilent 1260 HT Sample Prep System Operation Manual

1.6.2 Preventative Maintenance

Periodically inspect the power lead for signs of wear or damage. Contact Agilent’s Service

Department to obtain a replacement lead –it must be fitted with a ferrite to maintain EMC

compatibility.

1.6.3 Temperature

The Sample Preparation System is designed to control the temperature of the sample preparation

vials to temperatures up to 260 °C and therefore the metal hot plates, vials and the base of the hand

pipettor will be extremely hot during normal operation. Please handle these components carefully

wearing suitable protective gloves. Heat sensitive warning labels are affixed to the hot sections of

the instrument for your protection.

Beware Hot Surfaces

1.6.4 Toxic and Flammable Vapours

To prevent the risk of accumulation of toxic and/or harmful vapours, the system must be used in a

fume cupboard or suitably extracted area when operated at elevated temperatures and with organic

solvents. Additional vapour removal can be achieved by connecting the hood of the hot plate to an

extraction system via standard 2”(50 mm) flexible ducting.

1.6.5 Risk Reduction Procedures for Flammable Liquids

THE FOLLOWING PRECAUTIONS ARE EXTREMELY IMPORTANT

Keep any sources of ignition (e.g. sparks or naked flames) well away from hot solvents!

Always wear a grounded anti-static wrist strap when manipulating flammable liquids.

Ensure the temperature of solvents is always kept at least 10 °C below their boiling point.

Always use extraction or ventilation to minimise the concentration of flammable vapours.

Remove solvent reservoir containers and cover all vials before shaking.

The hood of the unit should be purged with Nitrogen during shaking.

Reduce the exposed surface area of solvents to a minimum by replacing vial caps and container

lids as quickly as possible.

Replace the hood whenever possible.

Do not overfill containers –allow plenty of room for solvent motion during shaking.

Use the minimum necessary quantity of solvents.

Mop up any spillages immediately.

Agilent 1260 HT Sample Prep System Operation Manual

2System Overview

The Agilent 1260 Infinity II High Temperature Sample Prep System is illustrated in figures 2.1a and 2.1b. It

comprises: -

A horizontal shaker [A]

Temperature controlled hot blocks [B] for sample dissolution in 20 mL vials.

The temperature control and shaking cycles are control from the keypad.[C]

The shaker speed is adjusted using the potentiometer [D]

Independent overtemperature safety cut-out system [E]

Power switch [F - located at the rear of the unit]

Removable extraction hood [G figure 2.1b] creating an oven environment for the samples and

has facilities for a nitrogen purge and vapour extraction.

Interchangeable blocks [H], the blocks with which contain the 2 mL vials are reversible

revealing a hole pattern for 4 mL autosampler vials.

A hand held pipettor [I] which is heated in the hot block when not in use.

Interchangeable frit magazines [J figure 2.2]

Figure 2.1a System Overview

Agilent 1260 HT Sample Prep System Operation Manual

Figure 2.1b System Overview

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