OPTO-EDU A14.1063 Manual de usuario

To ensure the safety and obtain satisfactory performance, please study this

instruction manual thoroughly before start to use your instrument.

A14.1063, A14.1064

Inverted Biological Microscope

A16.1063, A16.1064

Inverted LED Fluorescent Microscope

Instruction Manual

Contents

Before Using ……………………………………………………………………

Safety Symbol…………………………………………………………………

Safety Precautions ……………………………………………………………

Care and Maintenance…………………………………………………………

Chapter 1 Introduction …………………………………………………………

1.1 Technical Specifications …………………………………………………

1.1.1 Main Technical Specifications and Configuration……………………

1.1.2 Objective Parameters…………………………………………………

1.1.3 Electrical Parameters…………………………………………………

1.2 Components ………………………………………………………………

Chapter 2 Overview of Agencies ………………………………………………

Chapter 3 Regulation and Operation …………………………………………

3.1 Turning the Power on ……………………………………………………

3.2 Turning the Illumination switch on………………………………………

3.2.1 Manual Lighting Switch……………………………………………

3.2.2 ECO Switch (Optional functions) …………………………………

3.3 Adjusting the Illumination Brightness……………………………………

3.4 Specimen Placement and Stage Knob Adjustment………………………

3.4.1 Mechanical Stage……………………………………………………

3.4.2 Pallet…………………………………………………………………

3.4.3 Auxiliary Stage………………………………………………………

3.5 Adjusting the Interpupillary Distance………………………………………

3.6 Focusing on the Specimens ………………………………………………

3.6.1 Focus Knobs…………………………………………………………

3.6.2 Focus Knob Tight Adjustment ………………………………………

3.7 Adjusting the Diascopic Illumination Aperture Diaphragm………………

3.8 Switching the Objective……………………………………………………

3.9 Operation the Phase Contrast Microscope…………………………………

3.10 Operation the Emboss Contrast Microscope ……………………………

3.10.1 Condenser-side Emboss Contrast Slider……………………………

3.10.2 Eyepiece-tube-side Emboss Contrast Slider ………………………

3.11 Operating the Episcopic Illumination

Microscopy………………………………

3.11.1 Eprscopic Illumination………………………………………………

3.11.2 Fluorescence Filter Cube Turret ……………………………………

3.11.3 Filter Cube Switching Unit …………………………………………

3.11.4 Fluorescence Filter Cube……………………………………………

3.11.5 Fluorescence LED Unit ……………………………………………

3.12 Operating the LCD ………………………………………………………

3.12.1 The status of LCD …………………………………………………

3.12.2 Operating the illumination switch ………………………………

Chapter 4 Assembly………………………………………………………………

4.1 Installation diagram ……………………………………………………

4.2 Installation Steps ………………………………………………………

4.2.1 Installing the Condenser ……………………………………………

4.2.2 Installing the Phase Contrast Slider …………………………………

4.2.3 Installing the Moving Ruler …………………………………………

4.2.4 Installing the Objective ………………………………………………

4.2.5 Installing the Eyepiece ………………………………………………

4.2.6 Installing the LED Light Box ………………………………………

4.2.7 Installing the Camera…………………………………………………

4.3 Assembly Episcopic Illumination Microscopy…………………………………

4.3.1 Installing the Filter Cube ……………………………………………

4.3.2 Installing the Fluorescence LED Unit ………………………………

4.3.3 Replacing the Fuse …………………………………………………

Chapter 5 Maintenance and Storage……………………………………………

5.1 Cleaning …………………………………………………………………

5.1.1 Cleaning the Lenses …………………………………………………

5.1.2 Cleaning Parts other than Lenses ……………………………………

5.1.3 Cleaning off Immersion Oil …………………………………………

5.1.4 Decontaminating this Product ………………………………………

5.2 Storage ……………………………………………………………………

Chapter 6 Troubleshooting ……………………………………………………

6.1 Optical System ……………………………………………………………

6.2 Mechanical Parts …………………………………………………………

6.3 electrical Parts………………………………………………………………

Before Using

Safety Symbol

Symbol

Description

The surface is hot and cannot be touched with bare

hands.

Please read this manual carefully before using the

product. Incorrect usage can cause personal injury or

property damage.

The main power switch is turned on.

The main power switch is turned off.

Adjustment direction of illumination.

Ground connection.

ECO

People go lights out switch.

Up and down light switch.

Light switch.

Through the CE certification.

Lever in，Bino：Trino=100：0

Lever out，Bino：Trino=0：100

Safety Precautions

1. Be careful when opening box, prevent lens glass to stick to fingerprint,

perspiration other factors influence observation, prevent lens and other

attachment drop damaged.

2. Avoid placing the microscope in place with direct sunlight, high

temperature or high humidity, dust, and strong vibration. Ensure that

the stage is flat, horizontal and strong enough.

3. If a bacterial solution or water spatters spill on the stage, objective or

observing tube, immediately unplug the power cord. Then,wipe off any

solution or water with to keep the microscope dry. Otherwise, it could

damage the instrument.

4. To avoid blocking the natural convection air for cooling, make sure the

left, right, top, and rear of the microscope are at least 10 centimeters

away from the wall and other objects.

5. Grounding the machine to avoid lightning strike.

6. To ensure safety, always make sure the main switch is in "O"

(disconnecting) and cut off the power while waiting for the LED lights

and the lamp room to cool completely. Input voltage check: the input

voltage indicated on the back of the microscope is consistent with the

supply voltage, otherwise the microscope will be seriously damaged.

7. Use the our company dedicated wire.

8. When carrying this product, one hand should firmly hold it by gripping

the recess in the front lower part of the main body and the other holds

the recess in the rear of the main body.

★Do not hold any other parts (such as the upper part of the illumination pillar, focus

knobs, eyepiece tube, or stage) when carrying the microscope. Doing so might result in

dropping or failure of this microscope.

Care and Maintenance

1. All lenses have been calibrated, please do not disassemble by yourself.

2. The structure of objective converter and coarse and fine focus

mechanism are precise, please do not disassemble easily.

3. The instrument should be kept clean, have to regular removal of dust,

special attention should be paid to do not contaminate the optical parts

when cleaning.

4. The stain of the lens, such as fingerprints and grease can be wiped with

a small amount of ethyl ether (70%) and alcohol (30%) mixed solution.

Recess in the rear

Recess in the front lower part

5. Do not use organic solvents to clean the non-optical parts of the

microscope. Please use neutral detergents when cleaning.

6. When using, if the microscope be wet liquid, should immediately cut

off the power and dry.

7. Do not disassemble any part of the microscope, which will affect the

function or reduce the performance of the microscope.

8. The instrument should be placed indoor with cool and dry, covered the

dust cover when not in use. Cover up, must wait for the lamp room to

cool down.

9. The microscope usage environmental requirements:

a) For indoor use;

b) The environment temperature range: 10 ℃~ 35 ℃;

c) Maximum relative humidity: 80% at 31 ℃, linear temperature down

to 34 ℃is 70%, 60% at 37 ℃, 50% at 40 ℃.

10. Microscope to store and transport environment requirements:

a) the environment temperature range: - 40 ℃~ + 70 ℃

b) relative humidity range: 10% ~ 100%

c) atmospheric pressure range: 500 hpa ~ 1060 hpa

Because the solvent such as ether and alcohol are extremely flammable, do

not operate power switch of various electrical equipment when using, and keep away

from open fire, please ensure indoor ventilation.

Chapter 1 Introduction

1.1 Technical Specifications

1.1.1 Main technical specifications and configuration

Inverted Biological Microscope,

Inverted LED Fluorescent Microscope

A14.1063

A14.1064

A16.1063

A16.1064

Optical System

NIS60 Infinite Optical System

(F200)

●

Observation

Method

Bright Field,

●

Phase Contrast

●

○

Epi-Fluorscence

○

●

DIC 3D Emboss Contrast

○

Hoffman Phase Contrast

○

Head

Seidentopf Viewing Head, Inclined

At 45°, Interpupillary Distance

48-75mm, Eyepiece Tube

Dia.30mm

●

Eyepiece / F.O.V

EW10x/22mm, Diopter Adjustable,

Dia.30mm

●

EW15x/16mm, Diopter Adjustable,

Dia.30mm

○

EW20x/12mm, Diopter Adjustable,

Dia.30mm

○

Nosepiece

Quintuple Nosepiece, Dovetail

Interface

●

Coded Quintuple Nosepiece,

Dovetail Interface

●

LWD Infinity Plan

Objective

4x/0.10, W.D.30.0mm

●

○

10x/0.25, W.D.10.2mm

○

20x/0.40, W.D.12.0mm

○

40x/0.60, W.D.2.20mm

○

LWD Infinity Plan

Phase Contrast

Objective

4x/0.10, W.D.30.0mm

○

10x/0.25, W.D.10.2mm

●

○

20x/0.40, W.D.12.0mm

●

○

40x/0.60, W.D.2.20mm

●

○

LWD Infinity Plan

Semi-APO

Phase Contrast

Objective

4x/0.13, W.D.17.0mm

○

10x/0.3, W.D.7.4mm

○

20x/0.45, W.D.8.0mm

○

40x/0.60, W.D.3.6mm

○

20x/0.45, W.D.7.5-8.8mm With

Iris Adjustable

○

40x/0.60, W.D.3.0-4.4mm With

Iris Adjustable

○

60x/0.70, W.D.1.8-2.6mm With Iris

Adjustable

○

LWD Infinity

Plan

Semi-APO

Fluorescent

Objective

4x/0.13, W.D.17.0mm, Cover

Glass -

●

10x/0.30, W.D.7.4mm, Cover

Glass1.2mm

●

20x/0.45, W.D.8.0mm, Cover

Glass1.2mm

●

40x/0.60, W.D.3.3mm, Cover

Glass1.2mm

●

60x/0.70, W.D.1.8-2.6mm, Cover

Glass1.2mm

○

Focusing System

Coaxial Coarse & Fine

Adjustment, Tension Adjustable

(At Right Hand), Fine Division

0.002mm, Coarse Focusing

Range Up 7mm, Down 1.5mm,

Max Up To 18.5mm After

Removing Focusing Limit.

●

Working Stage

Plain Working Stage 170(X) x

250(Y)mm,

●

Attachable Mechanical Moving

Stage, X-Y Coaxial Moving

128x80mm

●

Stage Clip

●

Well Clamper For Well Plate

●

Glass Stage Plate

●

Metal Stage Plate For Culture

Bottle

○

Auxilliary Plate 2 Pieces (1 Piece

Each Side)

●

Universal Holder

●

Terasaki Holder

○

Dia.35mm Petri Dish Holder

○

Dia.54mm Slide & Petri Dish

Holder

○

Dia.65mm Slide & Petri Dish

Holder

○

Dia.90mm Petri Dish Holder

○

Condenser

Long Working Distance

Detachable Condenser NA

0.3, W.D.75mm, Without

Condenser W.D.187mm

●

Illumination

Transmitted Illumination 3W

S-LED Critical Illumination

●

Transmitted Illumination 3W

S-LED Kohler Illumination

●

Reflected Illumination 3W LED,

For Epi-Fluorescence

○

●

Phase Contrast

Centering Telescope 10x, Tube

Dia. 30mm

●

○

Phase Slider For 4x//10x-20x-40x

●

○

Phase Slider For 10x-20x,40x

APO Objectives

○

Phase Slider For 4x,60x APO

Objectives

○

Emboss Contrast

DIC

Emboss Contrast Slider For

10x-20x-40x

Universal Emboss Contrast Plate

For 10x-20x-40x

○

Hoffman Phase

Contrast

Hoffman Phase Contrast Set:

--Hoffman Phase Contrast

Condenser, With Polarizer,

--Hoffman Phase Contrast

Objective 10x, 20x, 40x

○

--Hoffman Phase Slider For 10x,

20x, 40x

--Centering Telescope 10x, Tube

Dia. 30mm

Epi-Fluorescence

Attachment

Epi-Fluorescence Attachment,

Turret With 3 Holes For Filter

Cubes, With Noise Terminator

Mechanism, With Attachable UV

Shield

○

●

Filter Cube B + LED Unit, 365nm

○

●

Filter Cube G + LED Unit, 405nm

○

●

Filter Cube U + LED Unit, 485nm

○

●

Filter Cube V + LED Unit, 525nm

○

Filter Cube FITC

Filter Cube DAPI

Filter Cube TRITC

Photo Port

Head Side Camera Port

Switchable 100/0:0/100

●

Photo Adapter

1.0x C-Mount

●

0.5x C-Mount

○

0.7x C-Mount

○

Power Supply

AC 100-240V,50/60Hz

●

ECO Function

Auto Power Off 15 Minutes After

No Operator Working

○

LCD Screen

LCD Screen On Front Of Body,

Display Using State Of

Microscope, Including

Magnification, Light Intensity,

Standby Status, ECO Set Power

Off Timer 5 Mins to 8 Hours, And

So On.

●

Dimensions

244(W)x543(D)x526(H) mm

●

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