OPTO-EDU A12.1010 Manual de usuario

A 1 2 . 1 0 1 0

Laboratory Biological Microscope

Instruction Manual

Please read this manual carefully before use microscope!

Contents BM2000

1. Structure and nomenclature -------------------------------------------------------4

2. Technical parameter and specification ------------------------------------------6

3. Configuration -----------------------------------------------------------------------8

4. Assemblage -------------------------------------------------------------------------9

5. Operation --------------------------------------------------------------------------13

6. Troubleshooting -------------------------------------------------------------------18

7. Maintenance -----------------------------------------------------------------------20

8. Products’ Standards --------------------------------------------------------------20

Safety notice

1. Transportation

As microscope is a precision instrument, handle with care,

avoiding impact or abrupt movement during transportation.

Do not push or pull the microscope during using, otherwise

the precision for image will be reduced.

1.1 Hold the curve and keep the microscope in balance

1.2 Do not hold the focusing knob, eyepiece tubes and stage

as these parts are movable. Troubles maybe caused by such

handles.

Do not make specimen or filters fall off.

2. Working Environment

As microscope is a precision instrument, improper using will make it unworkable or reduce its precision.

2.1 Do not expose the microscope in the sun directly

2.2 Temperature range is 0℃～40℃ and the max. humidity is 85%

2.3 Avoid high temperature and humidification otherwise there will be fog or mold on the lens

2.4 Avoid violent vibration as the vibration will reduce the image’s quality

2.5 Place microscope on a stable plane and keep it in balance. Keep the environment breezy and cover the

microscope with the dust-cover

2.6 Do not place microscope in moist room to avoid short circuit. Please turn off the power supply as soon

as water drop in the microscope. If there are other things unsafe come into the microscope may cause short

circuit too, please stop using and contact with manufacturers

3. Focusing knob

Never turn the left and right focusing knob in the adverse

direction at the same time. Do not turn the coarse focusing

knob when the stage reaches max. position. These improper

operations will make focusing structure unworkable.

4. Clamp

The clamps fix the microscope firmly during transportation.

Please remove them before using.

5. Do not take the microscope apart as it will be damaged. Such operations might have bad effect on the

performance and make user get an electric shock or injured. Please contact with manufacturers if there is

any problem.

6. There is correct input voltage in the label on microscope.

Make sure the voltage in your position is right. Improper input

clamp

voltage may cause short circuit and fire. The microscope will

be damaged.

Input voltage in the label: 220～240V 50/60Hz

Working voltage could be AC 220V, 230V or 240V

7. The bulb, fuse and electronic cord have been assembled already in the factory and please make sure to

use the spare parts of them supplied by the manufacturers. Improper bulb, fuse and electronic cord will

destroy microscope and cause fire. Please make sure to use PE electronic cord when using extra-long

electronic cord.

Spare bulbs: halogen bulb 12V20W (or 12V30W)

Spare fuse: 250V 1A delayed model, 5 × 20 minitype fuse

8. Never touch the surface of bulb with your hand directly. Please use gloves or cloth material when you

mount the bulb to avoid leaving fingerprints. Fingerprints or stains should be wiped off with a tissue

moistened with a small amount of alcohol. The fingerprints will etch on the bulb’s surface and make

brightness lower and life cycle shorter.

Please make sure the bulb’s touching points are Ok as it will not light or short circuit if the bulb’s touching

points are damaged. Insert the bulb’s legs into the bulb holder firmly. The bulb will go out if its legs have

not been inserted into the holder firmly. Such Operation may cause short circuit or smoking. At last, please

make sure if the collector has been mounted properly

9. Temperature for illumination

There will be high temperature when the bulb is lightened, never move the collector when the bulb is

lightened. Do not touch the bulb within 30 minutes after it has been gout out. Make sure the bulb has been

be cooling enough time (at least 30 minutes) to avoid scald.

Do not put fibre, papers and incendive things (eg. Gasoline, aether, methanol and ethanol) close to the bulb.

10. Make sure to turn off the power supply before assembling microscope, replacing bulb or fuse.

11. Using small amount of oil immersion is enough. The redundant oil will adhere to stage or condenser

and these will reduce microscope’s performance.

Get rid of the redundant oil or clean the lens by using aether or pure alcohol according to this instruction.

Pay attention during process as these things are incentive

The instruction you buy may not including some products mentioned in this instruction. Safety has been

considered during design while users still have the possibility to be injured and the instruction to be

damaged when improper operation made. Please pay much more attention to read this instruction carefully

before using and keep it properly to make sure it will be got when it is necessary.

1. Structure and nomenclature

Eyepiece 10X,

diopter adjustable

Eyepiece fixture

screw

Nosepiece

Objectives

Specimen holder

Mechanical stage

Condenser with

auxiliary lens

Collector

Binocular head

Eye tubes fixture

Microscope body

Power supply

Lightness adjusting

knob switch screw

Fine focusing knob

Stage movement

knob (Y axis)

Stage movement

knob (X axis)

2. Technical parameter and specification

1. Specification

1.1 Optical system: infinity optical system

1.2 Illumination: inserted transmitted illumination, 12V20W/30W halogen bulb (standard outfit) or LED

(available)

Input voltage: 220V～240V 50/60Hz

Voltage undulation: ±10%

Electric current: 0.4A

Fuse: 250V 1A delayed model, 5×20 minitype fuse 2pcs

1.3 Focusing adjustment: Division of fine focusing adjustment: 0.002mm

Fine focusing knob control range: stage will be up or down 0.2mm per circle

Coarse focusing knob control range: stage will be up or down 20mm

1.4 Mechanical stage: X-movement range: 54mm, Y-movement range: 78mm

1.5 Nosepiece: Roller bear quadruple nosepiece

1.6 Condenser: Abbe NA=1.25 with iris diaphragm

1.7 Eye tubes: Interpupillary range 55～75mm

1.8 Working environment: Temperature: 0℃～40℃

Humidity: max. 85%, no dew

Altitude: within 2000m

Pollution: 2

Assembly: Ⅱ

2. Optical parameter

2.1 Objectives: infinity achromatic objectives

Magnification N.A. Cover glass

thinkness(mm)

W.D.

(mm)

Dry/Oil

4X 0.1 0.17 25 Dry

10X 0.25 0.17 5.6 Dry

40X 0.65 0.17 0.6 Dry

100X 1.25 0.17 0.14 Oil

2.2 Eyepiece: WF10X/20mm

Nomenclature

﹡Total magnification

Total magnification = Eyepiece’s magnification × objective’s magnification

﹡Numerical aperture (N.A.)

N.A. value will affect resolution and image’s brightness, it is the leading parameter for objectives. N.A.=

n × sinα

n stands for refractive index of the medium between objective and specimen or condenser (air or oil

immersion)

αstands for half angle of max. aperture angle in the axis

The image will be sharper and brighter when the N.A. value is bigger

﹡Resolution

Resolution can be measured by the distance value from one point to another one which could be

distinguished on the object surface

Resolution = λ / ( 2 × N.A.)

λ stands for wavelength ( λ = 0.55μmm)

﹡Eyepiece view field

The max diameter measured when the diaphragm is open. 10X/20 means the magnification is 10X and

the max diameter of view field is 20mm

﹡Effective view field

Liner view field which is observed on the object surface

Effective view field = eyepiece view field / objective’s magnification

﹡Depth of field

Depth of field stands for depth of the space in which there is sharp image on the object surface. Depth

of field will be longer when the diaphragm reduces. Depth of field will be shorter when the N.A.

becomes bigger.

3. Configuration

Item Content Piece

1 Microscope’s body ( including stage, nosepiece,

coaxial coarse and fine focusing adjustment,

bracket for condenser, adjusting power supply,

halogen bulb and normal collector)

1pc

2 Siedentopf binocular inclined at 30º(360º

rotatable)

1pc

3 Condenser ( with iris diaphragm, without

auxiliary lens)

1pc

Objectives

( Infinity achromatic

objectives)

1 set

10X

40X

100X

5 WF10X eyepiece 2 pcs

6 Blue filter 1 set

7 Electronic cord 1 pc

8 Immersion oil 1 bottle

9 Dust cover 1 pc

10 Instruction and quality certificate 1 pc

11 Halogen bulb ( 12V20W) 2 pcs

12 Wrench 2.5, 1.5 1pc

Optional

Item Contents Piece

1 Collector with diaphragm

2 Condenser ( with iris diaphragm and auxiliary

lens)

3 Dark field condenser

4 WF10X eyepiece with reticle

5 WF15X eyepiece (15mm)

Objectives

(Infinity plan

10X

objectives) 40X

100X

7 60X/0.85 achromatic objective

8 LED transmitted illumination

9 Siedentopf trinocular inclined at 30º(360º

rotatable)

4. Assemblage

Please read the safety notice carefully before assembly and make the assembly according to the following

steps.

Tools: wrench (2 pcs)

4.1 There is correct input voltage in the label on microscope.

Make sure the voltage in your position is right. Improper input

voltage may cause short circuit and fire. The microscope will

be damaged.

Turn off the power supply (turn the switch to “O”) and insert

one end of the electronic cord (connector) into the entrance for

AC. Insert the other end of the electronic cord (pin) into the

grounded AC connector and make sure that the electronic cord

has been connected safely

﹡Please use the supplied electronic cord by manufacturer

﹡Please make sure to use PE electronic cord when using extra-long electronic cord.

﹡Pay attention that the microscope should be placed near AC connector and the AC connector is

touchable for users

4.2 The clamps fix the stage and focusing adjustment firmly

during transportation.

Handle the grooves beside collector and pull it out. Remove

the clamps by wrenches.

﹡Mechanical stage

There is one clamp fixing the mechanical stage in Y axis

direction. Remove the bolts and clamps.

﹡Focusing adjustment

Pull out the collector. The bracket is fixed by one clamp.

Please remove the bolts and clamps.

4.3 Mount the binocular head

Loosen the fixing screw in the eye tubes and insert the

binocular head into the eye tubes. Then tighten the screw.

4.4 Mount the blue filter

Take out the filter from the bottom of condenser and mount

it in the bracket then move the bracket into the condenser.

4.5 Adjustment and replacement

4.5.1 Condenser

The condenser is mounted in the microscope before

shipment. Remove or replace the condenser according to the

following steps

4.5.1.1 Turn the lifting knob for condenser and make

the bracket in a suitable position

4.5.1.2 Loosen the screw in the left side of condenser

and take out the condenser. Mount the blue filter in the

bracket then move it into the bottom of the condenser. Make

the label in the condenser forward and move the condenser into the bracket. At last tighten the screw.

4.5.1.3 Turn the lifting knob of condenser and make the condenser to the highest position. The

image will be focused in the right place of object (center of light path) when the light

transit condenser. Move the condenser up and down a little to make the dispersion image

disappeared.

4.5.2 Objectives

The objectives have been mounted in the microscope before shipment

Take off the specimen from the stage and make the stage lower when you replace the objectives.

Handle the objective with your two hands and remove it. Be carefully do not make it fall off.

4.5.3 Specimen clip

The clip has been mounted in the stage before shipment

Loosen two screws by wrenches supplied by manufacturer to remove the clip.

4.5.4 Eyepieces

10X eyepieces have been in the foam box before shipment. Insert the eyepiece into the eyepiece

tubes and tighten the fixing screws by wrench

Handle the eyepieces and replace them according to following picture

Left and right eyepiece should be replaced together when replacing to use 15X eyepiece to keep

the same magnification between left and right eye tubes

Notice: The 10X eyepiece should touch the end of eye tube

The “O” scale should be consistent with standard line when the eyepieces are inserted in or

pulled out of the eye tubes. Handle the eyepiece cover and never handle the diopter adjusting

ring

4.5.5 Other attachments

Replace other attachments (eg, camera) according to the instruction

4.6 Replacement

4.6.1 Replacement for bulb

4.6.1.1 Turn off the power supply (turn the switch to “O”) and pull out the connector

4.6.1.2 Wait for 30 minutes till the bulb is cooling

4.6.1.3 Handle the grooves in condenser and pull it out

4.6.1.4 Pull out the original bulb

4.6.1.5 Replace the bulb by gloves or cloth material then

insert the bulb into the socket entirely

4.6.1.6 Pull the condenser back to its original position

4.6.1.7 Connect the electronic cord to the power supply

4.6.2 Replacement for fuse

4.6.2.1 Turn off the power supply (turn the switch to “O”)

and pull out the connector

4.6.2.2 Open the cover for fuse by screwdriver

4.6.2.3 Mount the new fuse

4.6.2.4 Make sure that the voltage shown in the cover for

fuse is same to the working voltage

4.6.2.5 Mount the cover

5. Operation

5.1 Illumination

Turn on the power supply (turn the switch to “-“) then

the bulb will be on. The lightness can be adjusted by turning the

adjusting knob.

5.2 Interpupillary range

Adjust the siendentipf binocular to make sure that view

field in the right and left eyepiece is consistent. The point “·”

stands for the interpupillary distance

5.3 Put the slide in the stage and make the cover glass on it.

Make the clips clamp them entirely. Two slides can be put in the

stage in the same time

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