Olympus U-UCD8A Manual de usuario

A X 7 3 5 5

INSTRUCTIONS

U-UCD8A

MOTORIZED UNIVERSAL CONDENSER

This instruction manual is for the Olympus Motorized Universal Condenser Model U-UCD8A. To ensure the

safety, obtain optimum performance and to familiarize yourself fully with the use of this condenser, we

recommend that you study this manual thoroughly before operating the microscope system. Retain this

instruction manual in an easily accessible place near the work desk for future reference.

Printed on 100% recycled paper with soy ink.

U-UCD8A

CONTENTS

IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-2

1 SYSTEM DIAGRAM 3

3-1 Preparation .................................................................................................................................................................................................................................................... 6

3-2 Brightfield Observation (BF) ............................................................................................................................................................................................ 6-8

3-3 Phase Contrast Observation (PH) ...................................................................................................................................................................... 9-10

3-4 Nomarski Differential Interference Contrast Observation (DIC) ........................................................................... 11- 15

3-5 Darkfield Observation (DF) ......................................................................................................................................................................................... 16-17

3-6 Simple Polarized Light Observation (KPO).......................................................................................................................................... 17- 18

6-1 Mounting the Top Lens ............................................................................................................................................................................................................ 23

6-2 Mounting the Optical Elements ........................................................................................................................................................................ 24-33

6-3 Mounting the Condenser ..................................................................................................................................................................................................... 34

6-4 Centering the Condenser.................................................................................................................................................................................................... 35

2 NOMENCLATURE 4-5

4 TROUBLESHOOTING GUIDE 19-21

5 SPECIFICATIONS 22

3 VARIOUS MICROSCOPY PROCEDURES 6-18

6 ASSEMBLY 23-35

IMPORTANT

· The U-UCD8A is a motorized universal condenser that can be used for advanced research applications

by combining it with the BX-UCB control box and U-HSTR2 hand switch (interlocking with other motor-

ized modules is also available by running the BX2 software on a PC).

· Simply by replacing the optical elements, the condenser permits a quick changeover between a variety of

microscopy under transmitted light, including the brightfield, darkfield, phase contrast, Nomarski differen-

tial interference contrast (DIC) and simple polarized light observations. The top lens can be swung in and

out to deal with objectives with low magnification (1.25X*) to high magnification (100X). In addition, brightfield

and Nomarski DIC observations using oil immersed objectives are also available by changing the top

lens with an oil immersed top lens.

* The U-FWT filter wheel and the U-FWCO1.25X low-magnification correction lens are required.

1Getting Ready

1. This manual pertains only to the motorized universal condenser unit. Before using this unit together with

the microscope (BX or BX2 series), make sure that you have carefully read and understand both manuals,

and understand how the two units should be used together.

2. This attachment is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe

impact.

3. Make sure that no dirt, fingerprints, etc. are left on the lens surface.

4. Avoid using excessive manual force to the motorized parts. Otherwise, they may be damaged.

5. Swing out the top lens before attaching or detaching the condenser to or from the microscope.

6. Be sure to center the condenser before use. At maximum decentration of the condenser, the top lens and

stage holder may interfere with each other, making swinging out of the top lens impossible.

7. Remove the condenser from the microscope before attaching or removing the optical elements.

8. Do not clamp the optical element centering screws too tightly.

9. If the optical element centering screws are tightened too much while no optical element is installed, it

may be impossible to return the screws to their original positions.

U-UCD8A

10. An intermediate tube or sliders may be necessary depending on the method of observation.

11. In brightfield observation, engage the optical elements (small) other than 1, 2 and 3 in the light path. The

field of view may be cut off if optical elements 1, 2 and/or 3 are used.

2Maintenance and Storage

1. To clean the lenses and other glass components, simply blow dirty away using a commercially available

blower and wipe gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially

available absolute alcohol.

Since the absolute alcohol is highly flammable, it must be handled carefully.

Be sure to keep it away from open flames or potential sources of electrical sparks —— for example,

electrical equipment that is being switched on or off.

Also remember to always use it only in a well-ventilated room.

2. Do not disassemble any part of the attachment as this could result in malfunction or reduced perfor-

mance.

3. The optical elements and index plates which are not used should be stored in a case.

SYSTEM DIAGRAM

}For the connection of connection cables, refer to the instruction manual for the BX-UCB control box.

Motorized Universal Condenser

U-UCD8A

* Necessary in DIC and simple polarized light observation using polarizer (U-FW32PO).

The U-FWCO1.25X low-magnification correction lane is required for observations using the 1.25X objective.

Motorized Transmitted

Light Filter Wheel

U-FWT*

Microscope

BX/BX2 series

Control Box

BX-UCB

Hand Switch

U-HSTR2

U-UCD8A

NOMENCLATURE

Connector

To be connected to the BX-UCB.

Turret cover clamping screw (Page 30)

Optical element index plate attaching

positions (Page 31)

x 8 positions.

Optical element index window

“{” shows the optical elements engaged

in the light path.

Allen screwdriver

Condenser centering

screws* (x 2) Condenser clamping screw* Stage clamping screw*

Top lens (Page 23)

The U-TLD dry type top lens

(provided as standard) may be

exchanged with the U-TLO oil

immersion type top lens (optional).

Optical element centering

knob (Pages 10 & 16)

Allen wrench (Page 33)

Can be attached by

magnetism to the

condenser bottom

surface (be sure to store

here to prevent loss).

* To improve the operability, replace the centering knobs and clamping knob of condenser holder and the clamping

knob of stage with these screws. (Some microscope models adopt screw tightening system.)

Optical Elements (For the applicable objectives, see pages 25 to 29.)

Phase Contrast Ring (Small)

U-PH1S

U-PH2S

U-PH3S

DIC Prism (Small)

U-DIC10S

U-DP10S

DIC Prism (Large)

Other DIC prism than the

U-DIC10S/U-DP10S.

Darkfield ring

U-DFA

Phase contrast ring (Large)

U-PH3

CAUTION · The turret has a dummy optical elements attached before factory shipment in order to prevent

loosening of the optical element centering/clamping screw. The dummy optical element can be

removed only when an optical element is to be attached to the turret.

· The dummy optical element (large) can accept a commercially available ND filter (having a frame

diameter of 25 mm). To attach an ND filter, place it in an idle position of the turret, fit the dummy

optical element (large) on it and attach the turret just like an ordinary optical element.

# When a reflective ND filter is used in simple polarized light observation, ghost may be

observed.

+0.3

+0.2

U-UCD8A

VARIOUS MICROSCOPY PROCEDURES

3-1 Preparation

· If the top lens and optical elements have not been assembled yet, first read Chapter 6, ASSEMBLY” (pages

23 to 35).

· Set the main switch of the BX-UCB control box to “ I” (ON) to make the microscope ready for observation.

For the operation of the BX-UCB and the U-HSTR2 hand switch, refer to their instruction manuals.

3-2 Brightfield Observation (BF)

Applicable objective power

Power 1.25X 2X 4X 10X 20X 40X 60X 100X

Brightfield (BF) ¦** ¦ ¦ ¦ ¦ ¦ ¦* ¦*

Top lens OUT Top lens IN

* The NA may slightly be insufficient when a dry type top lens is used. However, this does not pose problem

in normal observations.

** Attach the U-FWCO1.25X low-magnification correction lens to the U-FWT filter wheel and engage them in

the light path. The image may be cut by the top lens frame, but this does not cause problem in the TV

observation and photomicrography.

If the objective is used without the correction lens, the light in the peripheral area will be insufficient.

}When a transmitted light DIC slider (U-DICT, U-DICTS, etc.) is used in combination, pull out the slider until it

clicks in place to disengage the slider from the light path.

}When a reflected light analyzer (U-AN) is used in combination, pull out the analyzer until it clicks in place to

disengage the analyzer from the light path.

1. Select the BF brightfield observation light path (no optical element engaged).

2. Engage the objective to be used in the light path by rotating the revolving nosepiece.

3. When using a 2X or 4X objective, swing out the condenser’s top lens. Also open the aperture iris dia-

phragm.

}When the top lens is swung out, the microscope’s field iris diaphragm function as aperture iris diaphragm.

4. Place the specimen on the stage.

5. Move the stage up or down to bring the specimen into focus.

6. Reduce the field iris diaphragm opening until its image circumscribes the field of view.

7. Adjust the aperture iris diaphragm.

}If the slide glass is thicker than 1.2 to 1.4 mm, the image of the field diaphragm may remain fuzzy. When

performing photomicrography, use a side glass with a thickness between 0.9 and 1.2 mm whenever

possible.

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