Olympus U-UCD8 Manual de usuario

A X 7 3 5 3

INSTRUCTIONS

U-UCD8

UNIVERSAL CONDENSER

This instruction manual is for the Olympus Universal Condenser Model U-UCD8. To ensure the safety, obtain

optimum performance and to familiarize yourself fully with the use of this condenser, we recommend that you

study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily

accessible place near the work desk for future reference.

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Printed on 100% recycled paper with soy ink.

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U-UCD8

CONTENTS

IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-2

1 NOMENCLATURE 3-5

2-1 Brightfield Observation (BF) ........................................................................................................................................................................................... 6-8

2-2 Phase Contrast Observation (PH) ......................................................................................................................................................................... 8-9

2-3 Nomarski Differential Interference Contrast Observation (DIC)......................................................................... 10-14

2-4 Darkfield Observation (DF) ........................................................................................................................................................................................ 15-16

2-5 Simple Polarized Light Observation (KPO) ....................................................................................................................................... 16-17

5-1 Mounting the Top Lens............................................................................................................................................................................................................ 21

5-2 Mounting the Optical Elements ....................................................................................................................................................................... 22-30

5-3 Mounting the Condenser..................................................................................................................................................................................................... 31

5-4 Centering the Condenser ................................................................................................................................................................................................... 32

5-5 Indication Using Number Stickers ......................................................................................................................................................................... 33

2 VARIOUS MICROSCOPY PROCEDURES 6-17

4 SPECIFICATIONS 20

5 ASSEMBLY 21-33

3 TROUBLESHOOTING GUIDE 18-19

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IMPORTANT

This product is a universal condenser applicable in advanced research applications based on complex

combination of observation methods. By simply exchanging the optical elements, the condenser can be

used in a variety of microscopy under transmitted light, including the brightfield, darkfield, phase contrast,

Nomarski differential interference contrast (DIC) and simplified polarized light observations. The top lens

can be swung in and out to deal with objectives with low magnification (2X) to high magnification (100X).

In addition, brightfield and Nomarski DIC observations using oil immersed objectives are also available

by changing the top lens with an oil immersed top lens.

1 Getting Ready

1. This manual pertains only to the universal condenser unit. Before using this unit together with the micro-

scope (BX40, BX50, BX60, BX41, BX51, BX52, etc.), make sure that you have carefully read and understand

both manuals, and understand how the two units should be used together.

2. This attachment is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe

impact.

3. Make sure that no dirt, fingerprints, etc. are left on the lens surface.

4. Do not force any control beyond its built-in limit (stopper, click, etc.). Avoid using excessive force.

5. Swing out the top lens before attaching or detaching the condenser to or from the microscope.

6. Be sure to center the condenser before use. At maximum decentration of the condenser, the top lens and

stage holder may interfere with each other, making swinging out of the top lens impossible.

7. Remove the condenser from the microscope before attaching or removing the optical elements.

8. Do not clamp the optical element centering screws too tightly.

9. If the optical element centering screws are tightened too much while no optical element is installed, it

may be impossible to return the screws to their original positions.

10. An intermediate tube or sliders may be necessary depending on the method of observation.

11. In brightfield observation, engage the optical elements (small) other than 1, 2 and 3 in the light path. The

field of view may be cut off if optical elements 1, 2 and/or 3 are used.

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U-UCD8

1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe

with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%).

Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be

sure to keep these chemicals away from open flames or potential sources of electrical sparks ——

for example, electrical equipment that is being switched on or off. Also remember to always use

these chemicals only in a well-ventilated room.

2. Do not disassemble any part of the attachment as this could result in malfunction or reduced perfor-

mance.

3. The optical elements and index plates which are not used should be stored in a case.

2Maintenance and Storage

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NOMENCLATURE

Universal Condenser

Optical element index plate

mounting position (Page 29)

Aperture iris diaphragm

scale (Page 7)

Aperture iris diaphragm

lever (Page 7)

Optical element index window Top lens swing-out lever

Top lens (Page 21)

The standard, dry type top lens (U-TLD) can

be exchanged with the oil immersion type

top lens (U-TLO).

Turret

Stage clamping screw*

* For easier viewing of the optical element index window, replace the stage clamping knob with this screw.

(Note) : Used when the U-UCD8 is combined with the IX/IX2

Series or BX51WI microscope. (Page 33)

Number sticker attaching groove

Number index (guideline)

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U-UCD8

Polarizer clamping knob

Polarizer rotation knob (Pages 12 & 17)

1- plate (Page 14)

Top lens swing-out lever

Aperture iris diaphragm

lever (Page 7)

Polarizer handling knob

Optical element centering screws

(Pages 9 & 15)

Clamping knob

Accommodation position.

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Optical Elements (For the applicable objectives, see pages 22 - 27.)

Phase Contrast Ring (Small)

U-PH1S

U-PH2S

U-PH3S

DIC Prism (Small)

U-DIC10S

U-DP10S

DIC Prism (Large)

Other DIC rings than the

U-DIC10S/U-DP10S.

Darkfield ring

U-DFA

Phase contrast ring (Large)

U-PH3

Number sticker

Indication plate sheet

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U-UCD8

VARIOUS MICROSCOPY PROCEDURES

}If the top lens and optical elements have not been assembled yet, first read Chapter 5, “ASSEMBLY”

(pages 21 to 30).

Applicable objective power

* The NA may slightly be insufficient when a dry type top lens is used. However, this does not pose

problem in normal observations.

#When a transmitted light DIC slider (U-DICT, U-DICTS, etc.) is used in combination, pull out the

slider until it clicks in place to disengage the slider from the light path.

#When a reflected light analyzer (U-AN) is used in combination, pull out the analyzer until it clicks in

place to disengage the analyzer from the light path.

1. Rotate the turret to select the BF brightfield observation light path (no optical element engaged).

2. Pull out the polarizer handling knob to disengage the polarizer from the light path.

3. Mount the objective to be used in the revolving nosepiece and rotate the nosepiece to swing the objec-

tive in place.

4. When using a 2X to 4X objective, swing out the condenser’s top lens. Also open the aperture iris dia-

phragm.

#When the top lens is swung out, the microscope’s field iris diaphragm function as aperture iris

diaphragm.

5. Place the specimen on the stage.

6. Move the stage up and down to bring the specimen into focus.

2-1 Brightfield Observation (BF)

Power 1.25X 2X 4X 10X 20X 40X 60X 100X

Brightfield (BF) – ¦ ¦ ¦ ¦ ¦ ¦* ¦*

Top lens OUT Top lens IN

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Field Iris Diaphragm

· The field iris diaphragm controls the size of the illuminated

area. By stopping down the field iris diaphragm, in accordance

with the objective in use, until its image circumscribes the

field of view, stray light can be reduced, which in turn increases

the definition and contrast of the image.

Aperture Iris Diaphragm

· The aperture iris diaphragm controls the numerical aperture

(N.A.) of the illuminator. In order to achieve the optimum objec-

tive performance, the opening of the aperture iris diaphragm

should be matched with the N.A. of the objective in use. This

will result in better image contrast and resolution as well as

increased depth of focus.

}When using an oil immersion type top lens, read the upper

graduations (marked “TLO”) on the aperture iris diaphragm

scale. When using a dry type top lens, read the lower gradua-

tions (marked “TLD”) on the aperture iris diaphragm scale.

Fig. 1

7. Reduce the field iris diaphragm opening until its image circumscribes the field of view.

8. Adjust the aperture iris diaphragm.

#If the slide glass is thicker than 1.2 to 1.4 mm, the image of the field diaphragm may remain fuzzy.

When performing photomicrography, use a side glass with a thickness between 0.2 and 1.2 mm

whenever possible.

Objective pupil

Aperture iris

diaphragm image 70-80%

30-20%

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