LLG uniSPEC 1 UV/VIS Manual de usuario
LLG-uniSPEC 1 UV/VIS
Spektrophotometer
Thank you very much for purchasing
this LLG-uniSPEC 1 UV/VIS.
Please read the following instructions carefully
and always keep this manual within easy reach.
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OPERATION MANUAL
OPERATION MANUAL EN
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Contents
1. Preface 3
1.1 Safety 3
1.2 General 3
1.3 Electrical 4
1.4 Warning 4
1.5 Performance 4
1.6 Radio Interference 4
2. Introduction of the Instrument 5
2.1 Brief Introduction of the instrument 5
2.2 Working Principle 6
2.3 Structure 6
2.4 Unpacking Instructions 7
2.5 Specications 7
3. Install Instrument 8
3.1 Environment Required 8
3.2 Installation 8
4. Operation Introduction 9
4.1 Software Structure 9
4.1.1 Main Interface 9
4.1.2 Firmware Functions 9
4.2 Basic Operation 10
4.2.1 Set Wavelength 10
4.2.2 Delete the test result and stored data 10
4.2.3 Calibrate 100%T/0Abs 10
4.2.4 Measure Samples 10
4.2.5 Print the test result 10
4.3 Preparation before test 10
4.3.1 Switch on the instrument and begin the system self-test 10
4.3.2 Pre-warm 10
4.3.3 Check the cuvettes 10
4.4 Measurement 11
4.4.1 T-Mode 11
4.4.2 A-Mode 12
4.4.3 C-Mode (Standard Curve) 12
4.4.4 F-Mode (Coefcient Method) 16
OPERATION MANUAL
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Contents
Read the following manual before installing and using the instrument and its accessories.
This instrument should be operated by appropriate laboratory technicians.
The apparatus described in this manual is designed to be used by properly trained personnel
in a suitable equipped laboratory. For the correct and safe use of this apparatus it is
essential that laboratory personnel follow generally accepted safe procedures in addition to
the safety precautions called for in this manual.
The covers on this instrument may be removed for servicing. However, the inside of the
power supply unit is a hazardous area and its cover should not be removed under any
circumstances. There are no serviceable components inside this power supply unit. For this
instrument, avoid touching the high voltage power supply at all times.
Some of the chemicals used in spectrophotometry are corrosive and/or inammable and
samples may be radioactive, toxic, or potentially infective. Care should be taken to follow
the normal laboratory procedures for handling chemicals and samples.
1. Preface
1.1 Safety
1.2 General
5 System Functions 18
5.1 Energy 18
5.2 Get Dark Current 19
5.3 Wavelength Calibration 20
5.4 Load Default 20
5.5 Version 21
6. Instrument Maintenance 21
6.1. Daily Maintenance 21
6.2. Trouble Shooting 22
6.3. Fuse replacement 22
7. Spare part and accessories 23
8. Warranty 24
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Before switching on the apparatus, make sure it is set to the voltage of the local power
supply (see Installation).
The power cord shall be inserted in a socket provided with a protective earth contact. The
protective action must not be negated by the use of an extension cord without a protective
conductor.
Any interruption of the protective conductor inside or outside the apparatus or disconnection
of the protective earth terminal is likely to make the apparatus dangerous. Intentional
interruption is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall be made
inoperative and be secured against any unintended operation.
Note:
NEVER touch or handle the power supply on this instrument due to the high voltage!
The protection is likely to be impaired if, for example, the apparatus
• Shows visible damage
• Fails to perform the intended measurements
• Has been subjected to prolonged storage under unfavorable conditions
• Has been subjected to severe transport stresses
To ensure that the instrument is working within its specication, especially when making
measurements of an important nature carry out performance checks with particular
reference to wavelength and absorbance accuracy. Performance checks are detailed in this
manual.
For compliance with the EMC standards referred to in the EC Declaration of Conformity, it is
necessary that only shielded cables supplied by us are used when connecting the instrument
to computers and accessories.
1.3 Electrical
1.4 Warning
1.5 Performance
1.6 Radio Interference
OPERATION MANUAL
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The LLG-uniSPEC 1 is a single beam, general purpose instrument designed to meet the
needs of the Conventional Laboratory, This instrument is ideal for various applications, such
as: Chemistry, Biochemistry, Petro-chemistry, Environmental Protection, Food and Beverage
Labs, Water and Waste Water Labs and other elds of quality control and research.
This instrument incorporates a 128 × 64 dots matrix LCD display for photometric results,
easy operation and wavelength range of 190 nm to 1000 nm. This instrument is ideal
for measurements in the visible and ultraviolet wavelength region of the electromagnetic
spectrum.
2. INTRODUCTION OF THE INSTRUMENT
2.1 Brief Introduction of the instrument
Fig 2-2 Control Panel
Fig 2-1 Front View
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Different matter has different but special absorbance wavelength point. Also, when at
the xed wavelength point, the absorbance has some relation to the substance’s (Always
transparent Solution) concentration and its thickness. The relation can be concluded as the
following Formula, which is called Lambert-Beer Law.
T= I/Io
A=KCL= -log I/Io
A Absorbance
C Concentration of the Solution
K Absorbance Coefcient of the Solution
L The length of the Solution in the light path
I The intensity of the light focused on the A/D after it permeate the solution to be
measured.
Io The intensity of the light focused on the A/D after it permeate the Solution.
Note: When the test is running, the solvent is usually taken as the Reference Solution and its
Transmittance is considered as 100%T. While the Transmittance of the sample to be tested is
a relative value which is going to be compared to that of the Reference.
The spectrophotometer consists of ve parts:
1) Halogen or deuterium lamps to supply the light;
2) A monochromator to isolate the wavelength of interest and eliminate the unwanted
second order radiation;
3) A sample compartment to accommodate the sample solution;
4) Detector to receive the transmitted light and convert it to an electrical signal; and
5) A digital display to indicate absorbance or transmittance. The block diagram (Fig 2.4)
below illustrates the relationship between these parts.
In your spectrophotometer, light from the lamp is focused on the entrance slit of the
monochromator where the collimating mirror directs the beam onto the grating. The grating
disperses the light beam to produce the spectrum, a portion of which is focused on the exit
slit of the monochromator by a collimating mirror. From here the beam is passed to a sample
compartment through one of the lters, which helps to eliminate unwanted second order
radiation from the diffraction grating. Upon leaving the sample compartment, the beam is
passed to the silicon photodiode detector and causes the detector to produce an electrical
signal that is displayed on the digital display.
2.2 Working Principle
2.3 Structure
Light
Scource
Monochromator DetectorSample
Compartment
Process and
Display
Fig 2.4 Block diagram for the Spectrophotometer
OPERATION MANUAL
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Carefully unpack the contents and check the materials against the following packing list to
ensure that you have received everything in good condition.
2.4 Unpacking Instructions
2.5 Specications
Packing List
Description Quantity
Spectrophotometer 1
Power Cord 1
1 cm Glass Cuvettes 1 Set of 4
1 cm Quartz Cuvettes 1 Set of 2
User’s Manual 1
Wavelength Range 190-1000 nm
Band Width 2 nm
Wavelength Accuracy ±2 nm
Wavelength Repeatability 0.5 nm
Wavelength Setting Auto
Photometric Accuracy ±0.5 %T
Photometric Repeatability ≤0.2 %T
Photometric Display Range 0-200 %T, -0.3-3.0A, 0-9999C
Stability 0.002 A/h @ 500 nm
Stray Light ≤0.1 %T
Data Output Port USB
Printer Port Parallel Port
Display 128*64 Dots LCD
Lamps Deuterium Lamp & Tungsten Halogen Lamp
Detector Silicon Photodiode
Power Requirement AC85~250 V
Dimension (L*W*H) 420*280*180 mm
Weight 11 kg
Note: The Printer mentioned in this manual is an optional accessory. It does not come
standard with the instrument.
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To ensure the best performance, the following conditions are required:
• The best working temperature range is 16-30 °C and the humidity is 45-80 % r.H.
• Keep it as far as possible away from the strong magnetic or electrical elds or any
electrical device that may generate high-frequency elds.
• Set the unit up in an area that is free of dust, corrosive gases and strong vibrations.
• Remove any obstructions or materials that could hinder the ow of air under and around
the instrument.
• The power requirement is AC85V~265V.
• Use the appropriate power cord and plug into a grounded outlet.
• If the local voltage is not stable enough, a Power Stabilizer is required.
• Keep away from direct sunlight
Step 1: Check the packing list
Unpack the contents, check the materials with the Packing List. Any damage or
item missing please contact us or the local dealer.
Step 2: Position
Place the instrument on a stable table carefully.
Step 3: Link the power cord
Make sure the instrument’s power switch is in the Off condition, link the power
cord to the instrument and insert another end in the socket provided with a
protective earth contact.
Step 4: Switch on the power
Check again, make sure that connections are correct. Switch on the power.
The instrument can be used for measurements after self-test and pre-warm.
3.1 Environment Required
3.2 Installation
3. Install Instrument
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After 20 minutes’ warm-up, the system goes into main interface. (Fig.4-1) Press the key of
MODE to change the functions.
The rmware consists of 5 functions: T, A, C, F and system functions
1) T
To measure the Transmittance of a sample in a xed wavelength point. The test result can be
stored in the Photometer, total 200 groups of data can be stored.
2) A
To measure the Absorbance of a sample in a xed wavelength point. The test result can be
stored in the Photometer, total 200 groups of data can be stored.
3) C-Standard Curve Method
Set up standard curve by standard samples; use the new curve to measure the unknown
sample’s concentration. The curves and the test results can be stored in the Photometer. Total
200 curves can be stored.
4) Coefcient Method
Input the values of the curve equation’s coefcient, and then measure the unknown sample’s
concentration.
5) Setup-System Functions
Press the key of SET on the keypad to go into system functions (Only available in T MODE
and A MODE). Light source management; Get Dark Current; Calibrate Wavelength and Load
default, etc.
4.1 Software Structure
4.1.1 Main Interface
4.1.2 Firmware Functions
4 Operation Introduction
Fig 4-1 Main Interface
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In the measurement Interface, press GOTO λto go into wavelength setting Interface. Use
Arrow Key to increase or decrease the value of wavelength, then press ENTER to conrm.
When arriving at the setting wavelength point, it sets 100%T/0Abs automatically.
In the measurement Interface, press CLEAR to delete the test result and stored data.
Put the Reference in the light path, press ZERO to calibrate 100%T/0Abs.
Put the samples in the light path and press ENTER to measure
In the Measurement Interface, press PRINT to print the result.
4.2 Basic Operation
4.2.1 Set Wavelength
4.2.2 Delete the test result and stored data
4.2.3 Calibrate 100%T/0Abs
4.2.4 Measure Samples
4.3.1 Switch on the instrument and begin the system self-test
4.3.2 Pre-warm
4.3.3 Check the cuvettes
4.2.5 Print the test result
Make sure to remove all the cuvettes in the light path; Close the compartment lid
and switch on the power; Then the system will begin self-test.
Note: During the course of Diagnostic Program runs, please DO NOT open the lid of
the compartment!
When the diagnostic program nished, it goes into pre-warm condition. 20 minutes is
required before measure.
The cuvette should be clear and no solution should remain on the transparent surface. If the
wavelength is below 340nm, you will have to use a Quartz Cuvette.
4.3 Preparation before test
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