Leica DMI6000 B Manual de usuario

DMI6000 instructions

The screen is not tactile!

On the right!!!

Fribourg, March 2009 Author: Sylvie DUCREUX

Your attention please: this manual is only a light version of the complete DMI6000

operating manual from Leica, if you need more details, please feel free to read it, one

copy is located inside our cupboard or ask me for the pdf file.

DMI6000 instructions

March 2009

Created by Sylvie Ducreux Page 2

Motorized platform = Z stage

SmartMove = the remote control element

Function keys on the right side of the stand

Z ↑to set the focus position, press at the same time Z↑

SET and SET (idem if you want to clear the previous set

Z ↓focus)

Light Adjustments

Defined function keys

1. Move in x-direction

2. Move in y-direction

3. Focus (= Z direction; but you’re free to use the regular wheel)

4. Height adjustment (you must not play with it, thanks!)

5. Variable function keys: Left XY fine Right Z fine

XY fast Z coarse

1. TL/IL: transmitted light/ incident light

2. AP: Aperture diaphragm

3. Light intensity (free increase with TL but fixed

step in IL= 10%, 17%, 30%, 55%, 100%)

4. Field diaphragm

DMI6000 instructions

March 2009

Created by Sylvie Ducreux Page 3

Objectives (see Special Notes)

You can exchange objective either by

1) pressing the bottom button to exchange between

DRY/IMMERSION then use the button or to

rotate the obejective turret to higher or smaller

magnification

Unlike the DM6000 screen, the DMI6000 Leicascreen is NOT tactile!!!

2) or directly turning the objective turret NOT RECOMMENDED!!!!

Avoid switching manually the objectives, as you may inadvertently rotate the objective

ring, or even worse, the objective itself.

3) Third alternative, you can also exchange objectives automatically inside the LAS-AF

software in the “Acquire” tab

Dry = x10

Immersion = x20 (water/glycerol with a special knurled ring) can be also use with

oil but need to be carefully cleaned after!

x63 blue ring for water immersion + additional knurled ring

x63 silver ring for glycerol immersion+ additional knurled ring

Notes:

The x63 objectives are common for both microscopes!

Meaning that if one is missing on one side, it must be on the other microscope, just unscrew it and

exchange it with the black plastic cache from the other side, don’t let the empty position open

exposed to dust!

Water and 80% are provided for common use and placed in the cupboard.

Nevertheless, please, don’t let stocks empty without informing the responsible person

Objectives must be cleaned after use…

!!! Attention!!! Inverted orientation carefully clean objective, revolver platform, especially if you

had some leakage

DMI6000 instructions

March 2009

Created by Sylvie Ducreux Page 4

Fluorescence = 2 cubes

N2.1

Additional cubes are available

CFP

YFP

Cubes can be exchanged by opening the drawer on the microscope right side.

If you don’t know how to do it, don’t try!

Ask for help!

(or see detailed protocol in the DMI6000 operating

manual)

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