Leica DMI6000B Manual de usuario

MICROSYSTEMS

Leica DMI3000 B

Leica DMI4000 B

Leica DMI6000 B

Operating Manual

Bedienungsanleitung

Phone/Tel. +49 (0) 64 41-29 22 86

Fax +49 (0) 64 41-29 22 55

E-mail/Email [email protected]

Published May 2005 by/

Herausgegeben Mai 2005 von:

Leica Microsystems Wetzlar GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Responsible for contents/

Verantwortlich für den Inhalt:

Bernard Kleine

(Marketing CMS, Life Science Research

Microscopy, Product Management)

Holger Grasse

(Safety Officer according to MPG §30/

Sicherheitsbeauftragter nach MPG §30)

In case of questions, please contact the hotline/

Bei Fragen wenden Sie sich bitte an die Hotline:

Leica DMI3000B

Leica DMI4000B

Leica DMI6000B

Operating Manual

Copyrights

All rights to this documentation are held by

Leica Microsystems Wetzlar GmbH. Reproduc-

tion of text or illustrations (in whole or in part) by

print, photocopy, microfilm or other method (in-

cluding electronic systems) is not allowed with-

out express written permission from Leica

Microsystems Wetzlar GmbH.

The term “Windows” may appear in the follow-

ing text without further identification. It is, how-

ever, a registered trademark of Microsoft Corpo-

ration. The names of companies and products

used herein may be trademarks of their respec-

tive owners.

The instructions contained in the following

documentation reflect state-of-the-art technol-

ogy and knowledge standards. We have com-

piled the texts and illustrations as accurately as

possible. Nevertheless, no liability of any kind

may be assumed for the accuracy of this manu-

al’s contents. Still, we are always grateful for

comments and suggestions regarding potential

mistakes within this documentation.

The information in this manual is subject to

modification at any time and without notifica-

tion.

Contents

6.11 Installation of Lamp Housing Mount

and Mirror Housing ................................... 42

6.12 Installation and Replacement

of Incident-Light Lamps............................ 44

6.13 Equipping the

Incident Light Turret Disk......................... 48

6.14 Inserting the Front Module Slider ..........

6.15 Installation of the Polarizer

and Analyzer............................................... 50

6.16 Optional Accessories ............................... 52

6.17 Connection to the

Electronics Box CTR6000 ......................... 53

6.18 Connection to the Computer ................... 54

6.19 Connection to the Power Supply ............ 54

7. Startup ......................................................... 55

7.1 Functional Principle .................................. 55

7.2 Switching On .............................................. 59

7.3 The LeicaScreen........................................ 60

7.4 The Function Buttons on the Stand ....... 61

7.5 The SmartMove Remote

Control Module .......................................... 64

7.6 Illumination ................................................. 65

7.6.1 Transmitted light ............................. 65

7.6.2 Incident Light – Fluorescence ..... 68

7.7 Checking Phase Contrast Rings ............. 69

7.8 Setting the Motorized Polarizer.............. 70

7.9 Adjusting the Light Sources .................... 71

Contents

1. Important Notes about this Manual ...... 7

2. Intended Purpose of the Microscope ... 8

3. Safety Notes ............................................... 9

3.1 General Safety Notes ............................... 9

3.2 Electrical Safety ........................................ 10

4. Overview of the Leica DMI6000 ............. 12

4.1 Specifications ............................................ 12

4.2 Glossary....................................................... 16

5. Unpacking the Microscope .................... 22

6. Assembling the Microscope .................. 25

6.1 Assembly Tools .......................................... 25

6.2 Installation of the

Transmitted-Light Illumination Carrier .. 26

6.3 Installation of the DIC Module

and DIC Objective Prisms ........................ 27

6.4 Installation of Stages ................................ 28

6.5 Installation of Condensers....................... 34

6.6 Installation of Eyepieces.......................... 39

6.7 Installation of Objectives ......................... 39

6.8 Installation of Filters

in the Illumination Arm ............................. 40

6.9 Installation of the

Transmitted-Light Lamp Housing............ 40

6.10 Installation and Replacement of the......

Transmitted-Light Lamps:.........................

Lamp Housing 107 or 107/2 ...................... 41

Contents

8. Operation .................................................... 74

8.1 Switching On .............................................. 74

8.2. Contrast Methods...................................... 76

8.2.1 Bright Field (TL) ............................... 76

8.2.2 Phase Contrast (TL) ........................ 77

8.2.3 Dark Field (TL) ................................. 77

8.2.4 Polarization (TL) .............................. 78

8.2.5 Differential

Interference Contrast (TL) ............ 79

8.3 Fluorescence.............................................. 80

8.4 Combination Methods .............................. 81

8.5 Focusing ...................................................... 82

8.6 Tubes............................................................ 84

8.7 Eyepieces .................................................... 85

8.8 Objectives ................................................... 85

8.9 Stages and Object Displacement ........... 88

8.10 Magnification Changer............................. 89

8.11 Light Sources ............................................. 89

8.12 Aperture and Field Diaphragm................ 90

9. Troubleshooting......................................... 91

10. Care of the Microscope ........................... 95

10.1 Dust Cover .................................................. 95

10.2 Cleaning....................................................... 95

10.3 Handling Acids and Bases ...................... 96

11. Essential Wear and Spare Parts ............ 97

12. Dimensions................................................. 98

13. Abbreviations and Pictograms............... 99

14. Index ............................................................ 101

15. EU Declaration of Conformity ................. 104

1. Important Notes about this Manual

(1.2)

→

p. 20

Numbers in parentheses, such as “(1.2)”, corre-

spond to illustrations (in the example, Figure 1,

Item 2).

Numbers with pointer arrows (for example

→ p. 20), point to a certain page of this manual.

Caution!

Special safety instructions within this

manual are indicated with the triangle sym-

bol shown here, and have a gray back-

ground.

Caution! The microscope and accessories can

be damaged when operated incorrectly.

Explanatory note.

Item not contained in all configurations.

Text symbols, pictograms and their meanings:

Caution!

This operating manual is an essential com-

ponent of the microscope, and must be read

carefully before the microscope is assem-

bled, put into operation or used.

1. Important Notes about this Manual

This operating manual contains important in-

structions and information for the operational

safety and maintenance of the microscope and

accessories. It must therefore be kept safely for

future reference.

A separate manual is available on CD-ROM cov-

ering the operation of the Leica Application

Suite (LAS).

2. Intended Purpose of the Microscope

The microscopes of the Leica DMI series cov-

ered in this manual are designed for biological

routine and research applications. This includes

the examination of samples taken from the hu-

man body with a view to providing information

on physiological or pathological states or con-

genital abnormalities, or to determining the

safety and compatibility with potential recipi-

ents, or to monitoring therapeutic measures.

The Leica DMI series is a further development of

Leica’s proven inverted research microscopes.-

It is designed for cellular and tissue examina-

tion, micromanipulation and microinjection

techniques, microdissection and confocal

microscopy. The Leica DMI series is suitable for

universal deployment. All contrast methods

such as dark field, bright field, phase contrast,

DIC, fluorescence (not DMI 3000) and modula-

tion contrast are integral to the microscope and

can be adapted or changed quickly and easily.

Variable illumination and imaging beam paths,

as well as HCS optics, modular accessories and

a comprehensive range of peripherals comple-

ment the Leica Microsystems inverted research

stand.

The above-named microscope complies with

the Council Directive 98/79/EEC concerning in

vitro diagnostics. They also conform to the

Council Directives 73/23/EEC concerning electri-

cal apparatus and 89/336/EEC concerning elec-

tromagnetic compatibility for use in an industrial

environment.

Caution!

The manufacturer assumes no liability for

damage caused by, or any risks arising from

using the microscopes for other purposes

than those for which they are intended or

not using them within the specifications of

Leica Microsystems Wetzlar GmbH.

In such cases the declaration of conformity

shall cease to be valid.

Caution!

This (IVD) device is not intended for use in

the patient environment defined by DIN VDE

0100-710. Neither is it intended for combin-

ing with medical instruments according to

EN 60601-1. If a microscope is electrically

connected to a medical instrument accord-

ing to EN 60601-1, the requirements defined

in EN 60601-1-1 shall apply.

3. Safety Notes

3.1 General Safety Notes

This safety class 1 device is constructed and

tested in accordance with

EN 61010-2-101:2002,

EN 61010-1:2001,

IEC 1010-1:2001,

Safety regulations for electrical measuring, con-

trol, and laboratory devices.

Caution!

In order to maintain this condition and to en-

sure safe operation, the user must follow the

instructions and warnings contained in this

operating manual.

Caution!

The devices and accessories described in

this operating manual have been tested for

safety and potential hazards.

The responsible Leica affiliate or the main

plant in Wetzlar must be consulted when-

ever the device is altered, modified or used

in conjunction with non-Leica components

that are outside of the scope of this manual.

Unauthorized alterations to the device or

noncompliant use shall void all rights to any

warranty claims!

3. Safety Notes

Caution!

Power plugs may only be plugged into an

outlet equipped with a grounding contact.

Do not interfere with the grounding function

by using an extension cord without a ground

wire. Any interruption of the ground wire in-

side or outside of the device, or release of

the ground wire connection, can cause the

device to become hazardous. Intentional

ground interruption is not permitted!

Caution!

Peripheral devices with their own or sepa-

rate power supplies that are connected to

the microscope can have the same protec-

tive conductor potential by connecting them

to the ground screw on the back of the Leica

CTRxxxx electronics boxes. For connections

without a ground connector, Leica Service

must be consulted.

Caution!

Never use any fuses as replacements other

than those of the types and the current rat-

ings listed here. Using patched fuses or

bridging the fuse holder is not permitted. The

use of incorrect fuses may result in a fire

hazard.

3.2 Electrical Safety

General specifications

Leica CTR4000, CTR6000 and CTR6500 electron-

ics boxes

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

Microscope

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

ebq 100 supply unit*

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

(see enclosed manual)

90–250 V~

50–60 Hz

max. 290 VA

T6.3 A

(IEC 60127-2/3)

15–35°C

max. 80% to 30°C

90–250 V~

50–60 Hz

see CTRxxxx

15–35°C

max. 80% to 30°C

90–250 V~

50–60 Hz

max. 155VA

2xT2A (IEC 127)

10–36°C

max. 80% to 30°C

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