Leica ASP300 S Manual de usuario

Leica ASP300 S

Advanced Smart Processor

Automated vacuum

tissue processor

Operating Manual

Leica ASP300S

V1.0 English - 07/2006

Always keep this manual together with the instrument.

Read this instruction manual carefully before working

with the instrument.

Leica ASP300 S

The information, numerical data, notes and value

judgments contained in this manual represent

the current state of scientific knowledge and

state-of-the-art technology as we understand it

following thorough investigation in this field.

We are under no obligation to update the present

manual according to the latest technical devel-

opments, nor to provide our customers with addi-

tional copies, updates etc. of this manual.

For erroneous statements, drawings, technical

illustrations etc. contained in this manual we

exclude liability as far as permissible according

to the national legal system applicable in each

individual case. In particular, no liability whatso-

ever is accepted for any financial loss or conse-

quential damage caused by or related to compli-

ance with statements or other information in this

manual.

Statements, drawings, illustrations and other

information as regards contents or technical

details of the present manual are not to be

considered as warranted characteristics of our

products.

These are determined only by the contract provi-

sions agreed between ourselves and our cus-

tomers.

Leica reserves the right to change technical

specifications as well as manufacturing process-

es without prior notice. Only in this way is it pos-

sible to continuously improve the technology and

manufacturing techniques used in our products.

This document is protected under copyright

laws. Any copyrights of this document are

retained by Leica Microsystems Nussloch GmbH.

Any reproduction of text and illustrations (or of

any parts thereof) by means of print, photocopy,

microfiche, web cam or other methods – including

any electronic systems and media – requires

express prior permission in writing by

Leica Microsystems Nussloch GmbH.

For the instrument serial number and year of

manufacture, please refer to the name plate at

the back of the instrument.

NOTE

Published by:

Leica Microsystems Nussloch GmbH

Heidelberger Str. 17 - 19

D-69226 Nussloch

Germany

Telephone: +49 (0)6224 143-0

Fax: +49 (0)6224 143-200

Internet: http://www.histo-solutions.de

4Operating Manual V 1.0 – 07/2006

Table of Contents

1. Important information ................................................................................................................. 6

2. Safety .............................................................................................................................................. 7

2.1 Safety instructions .................................................................................................................................... 7

2.2 Warnings .................................................................................................................................................... 7

2.3 Integrated safety devices ...................................................................................................................... 11

3. Instrument components and specifications ......................................................................... 12

3.1 Overview - instrument components..................................................................................................... 12

3.2 Specific instrument options .................................................................................................................. 14

3.3 Standard delivery - packing list ............................................................................................................ 15

3.4 Technical Data ........................................................................................................................................ 16

3.5 Compatible reagents .............................................................................................................................. 19

4. Setting up the instrument ......................................................................................................... 20

4.1 Site requirements ................................................................................................................................... 20

4.2 Electrical connection ............................................................................................................................. 20

4.3 Installing the accessories ..................................................................................................................... 22

4.4 Setting up the data connections .......................................................................................................... 23

4.5 Alarm functions ....................................................................................................................................... 24

4.6 Switching the instrument on ................................................................................................................. 25

4.7 Touchscreen functions .......................................................................................................................... 27

4.8 Checklist for first operation ................................................................................................................... 28

5. Operation ..................................................................................................................................... 29

5.1 Setting up the instrument parameters ................................................................................................. 29

5.1.1 System setup ........................................................................................................................................... 29

5.1.2 Access levels .......................................................................................................................................... 32

5.1.3 INSTALLATION menu ............................................................................................................................. 34

5.1.4 Editing the reagent list ........................................................................................................................... 37

5.1.5 Viewing the program list ........................................................................................................................ 40

5.1.6 Adding and/or modifying programs ..................................................................................................... 41

5.1.7 Favorites ................................................................................................................................................... 44

5.1.8 Editing the stations ................................................................................................................................. 46

5.1.9 Reagent groups ....................................................................................................................................... 47

5.2 Reagent handling .................................................................................................................................... 48

5.2.1 Fill/drain reagents (other than wax) ..................................................................................................... 48

5.2.2 Changing the wax ................................................................................................................................... 50

Leica ASP300 S

Table of Contents

5.3 Running programs .................................................................................................................................. 53

5.3.1 Editing a selected program ................................................................................................................... 54

5.3.2 Starting a program .................................................................................................................................. 56

5.4 Reagent status ........................................................................................................................................ 57

5.5 System Monitor ....................................................................................................................................... 58

5.6 On-line help .............................................................................................................................................. 59

6. Solutions ...................................................................................................................................... 60

6.1 General ..................................................................................................................................................... 60

6.2 Power failure ........................................................................................................................................... 60

6.3 Troubleshooting ...................................................................................................................................... 61

6.4 Typical fill or drain problems................................................................................................................. 62

7. Cleaning and Maintenance ..................................................................................................... 63

7.1 Clean programs ....................................................................................................................................... 63

7.1.1 Retort clean programs ........................................................................................................................... 63

7.1.2 Wax clean program ................................................................................................................................ 65

7.1.3 Smart Clean ............................................................................................................................................. 66

7.2 General cleaning steps .......................................................................................................................... 67

7.2.1 Daily cleaning and maintenance .......................................................................................................... 69

7.2.2 Periodic cleaning and maintenance .................................................................................................... 71

7.3 Preventative maintenance schedule ................................................................................................... 72

8. Warranty and Service ............................................................................................................... 74

6Operating Manual V 1.0 – 07/2006

Symbols used in this manual

Warnings

appear in a gray box and are

marked by a warning triangle .

Notes,

i.e. important user information ap-

pear in a gray box and are marked

by an information symbol .

Flammable solvents and reagents

are marked with this symbol.

Instrument surfaces which become

hot during operation are marked

with this symbol.

Avoid direct contact with these sur-

faces - they may cause burns.

Figures in brackets refer to item

nos. in drawings.

Function keys to be pressed on the

instrument touch screen are written

in bold-print capital letters.

1. Important information

Designated use

The instrument has been designed so that it is

safe to use by the operator as well as for pro-

cessing specimens – provided that it is operated

according to the present instruction manual.

The Leica ASP300S is a modular automated

tissue processor designed for the following labo-

ratory applications:

• fixation,

• dehydration

• paraffin wax infiltration of histological

tissue specimens.

The Leica ASP300 S must be operated exclusively

with the reagents listed

in chapter 3.5 - "Compatible reagents" .

(5)

ENTER

Qualification of personnel

• The Leica ASP300 S may be operated only by

trained laboratory personnel.

•

The instrument may be operated only accord-

ing to the instructions contained in this manual.

Instrument type

All information provided in this manual applies

only to the instrument type indicated on the title

page.

Fig. 1

A name plate

indicating the

instrument

serial number

is attached to

the back of the

instrument.

Any other use of the instrument is

considered improper!

Failure to adhere to these instruc-

tions may result in an accident,

personal injury, damage to the

instrument or accessory equipment.

Leica ASP300 S

2. Safety

2.1 Safety instructions

This instruction manual includes important infor-

mation related to the operating safety and main-

tenance of the instrument and it is an important

part of the product.

If additional requirements on acci-

dent prevention and environmental

protection exist in the country of op-

eration, this instruction manual

must be supplemented by appropri-

ate instructions to ensure compli-

ance with such requirements.

Make sure to comply with the safety instructions and warnings in this chapter.

Make sure to read these instructions, even if you are already familiar with the operation

and use of other Leica products.

The protective devices on both instrument and accessories may neither be removed nor

modified. Only authorized and qualified service personnel may repair the instrument and

access the instrument’s internal components.

2.2 Warnings

The safety devices installed in this instrument by the manufacturer only constitute the basis for

accident prevention. Primarily responsible for accident-free operation is above all the institution

which owns the instrument and, in addition, the designated personnel who operates, services or

repairs the instrument.

To ensure trouble-free operation of the instrument, make sure to comply with the following

instructions and warnings.

This instrument has been built and tested in ac-

cordance with the safety regulations on electri-

cal measuring, control, regulating and laboratory

devices.

In order to maintain this condition and to ensure

safe operation the operator must comply with the

instructions and warning contained in this

instruction manual.

For current information on applicable

standards, please refer to the instru-

ment’s CE declaration and visit:

http://www.histo-solutions.com

8Operating Manual V 1.0 – 07/2006

2. Safety

Transport and Installation

The instrument may only be transported in an upright position.

Follow the unpacking instructions carefully to avoid damage to the instrument!

A cleaning must be performed prior to each transport, during which the instrument may

be shaken, tilted or lifted. Severe internal damage may otherwise occur.

The instrument MUST be connected to an earthed mains power outlet socket. The instru-

ment must not be connected to an extension cord without protective earth conductor.

Ensure uniform current supply according the to set voltage!

The set voltage CANNOT be changed by the user.

Severe damage may occur if the instrument is connected to a power supply voltage other

than that to which it was originally set.

The instrument must be set up in a well ventilated area, free from any ignition sources.

The chemicals to be used in the Leica ASP300 S are both flammable and noxious.

Do not operate the instrument in rooms with explosion hazard.

If there is a significant difference in temperature between the warehousing and the in-

stallation site of the instrument and if at the same time there is a high air humidity level,

condensation water may form. In this case, a waiting period of at least two hours must be

observed before the instrument is switched on. Failure to adhere to this waiting period

may result in damage to the instrument. Failure to adhere to this waiting period may re-

sult in damage to the instrument.

Warnings - Markings on the instrument itself

Markings on the instrument showing the warning triangle indicate that the correct

operating instructions (as defined in this manual) must be followed when operating or

replacing the item marked. Failure to adhere to these instructions may result in an acci-

dent, personal injury, damage to the instrument or accessory equipment.

Some instrument surfaces become hot during operation.

They are marked with this warning label. Touching these surfaces may cause

burns.

Leica ASP300 S

Warnings - Operating the instrument

The Leica ASP300S may only be operated by trained laboratory personnel, according to

its designated use and per the present instruction manual.

The ON/STOP switch at the side of the instrument may be used in an emergency to stop

the instrument during operation.

Always press PAUSE before opening the retort lid during processing to allow the retort to

vent.

Do not remove the wax drain hose or the remote fill/drain hose until the fill or drain has

been completed, as pressurized air is used to clear the hose after each fill / drain.

After refilling / replacing the reagent bottles ensure that the lids are tight.

The bottles must be properly pushed home into the connection manifolds at the rear inner

wall of the reagent module.

Failure to correctly plug the reagent bottles into the manifold will cause an interruption

to the processing run and may result in spilling of reagents.

Fixatives containing mercuric salts, acetic or picric acid will corrode metallic compo-

nents in the instrument.

After each paraffin step a retort clean cycle must be run.

2. Safety

Material safety data sheets can be obtained from the supplier of the chemicals.

They are also available on the Internet:

http://www.msdsonline.com

10 Operating Manual V 1.0 – 07/2006

Warnings -handling reagents

Be careful when handling solvents!

Always wear rubber gloves and safety goggles when handling the chemicals used in this

instrument.

Reagents used for tissue infiltration can be both toxic and/or flammable.

To avoid damaging the instrument only use those reagents that are listed in chapter 3.5!

Do not use acetone, benzene or trichlorethane in the instrument!

Use caution when handling paraffin wax or removing baskets – molten paraffin is hot and

may cause burns.

Also, avoid personal contact with paraffin stations and retort walls – they can be very hot

as well.

Dispose of waste solvents with care according to local regulations and the waste man-

agement policy of the company or institution.

Do not clean reagent bottles in an automatic dishwasher – they are NOT dishwasher-

proof.

2. Safety

Warnings - Cleaning and maintenance

Prior to each maintenance and/or cleaning, switch the instrument off and disconnect

mains power.

Do not clean the instrument with solvents containing acetone or xylene. No liquid may be

spilled into the internal components of the instrument – neither during operation nor

during cleaning.

When working with cleaning detergents, comply with all safety instructions by the manu-

facturer of the product and the laboratory management policy.

The condensate bottle should be inspected at least once every week and, if necessary,

emptied.

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