Leica VT1000 S Manual de usuario

Instructions for Use

VT1000 S

Vibrating-blade microtome

Leica VT1000 S, V 2.3 , English - 09/2018

Order No. 14 0472 80101 RevK

Always keep this manual with the instrument.

Read carefully before working with the instrument.

Leica VT1000 S - Vibrating-blade microtome

These are determined only by the contract

provisions agreed between ourselves and our

customers.

Leica reserves the right to change technical spec-

ifications as well as manufacturing processes

without prior notice. Only in this way is it pos-

sible to continuously improve the technology and

manufacturing techniques used in our products.

This document is protected under copyright laws.

Any copyrights of this document are retained by

Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of

any parts thereof) by means of print, photocopy,

microfiche, web cam or other methods – including

any electronic systems and media – requires

express prior permission in writing by Leica

Biosystems Nussloch GmbH.

For the instrument serial number and year of

manufacture, please refer to the nameplate at

the rear side of the instrument.

The information, numerical data, notes and value

judgments contained in this manual represent

the current state of scientific knowledge and

state-of-the-art technology as we understand it

following thorough investigation in this field. We

are under no obligation to update the present

manual periodically and on an ongoing basis ac-

cording to the latest technical developments, nor

to provide our customers with additional copies,

updates etc. of this manual.

To the extent permitted in accordance with the

national legal system as applicable in each indi-

vidual case, we shall not be held liable for errone-

ous statements, drawings, technical illustrations

etc. contained in this manual.

In particular, no liability whatsoever is accepted

for any financial loss or consequential damage

caused by or related to compliance with state-

ments or other information in this manual.

Statements, drawings, illustrations and other

information as regards contents or technical

details of the present Instructions for Use are not

to be considered as warranted characteristics of

our products.

Note

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19

D-69226 Nussloch

Germany

Phone: +49 6224 143-0

Fax: +49 6224 143-268

Internet: http://www.LeicaBiosystems.com

Assembly contracted to Leica Microsystems Ltd. Shanghai

2Instructions for Use, V 2.3 RevK – 09/2018

Contents

1. Important Information ....................................................................................................3

2. Safety ................................................................................................................................5

2.1 Safety notes................................................................................................................................... 5

2.2 Warnings........................................................................................................................................ 5

2.3 Safety instructions for handling the instrument...................................................................... 6

3. Instrument Characteristics ...........................................................................................7

3.1 Technical Data.............................................................................................................................. 7

3.2 General overview - VT1000 S...................................................................................................... 8

4. Installation.....................................................................................................................10

4.1 Standard delivery........................................................................................................................ 10

4.2 Unpacking and setting up the instrument............................................................................... 11

5. Operation........................................................................................................................13

5.1 Installation site requirements................................................................................................... 13

5.2 Setting up the instrument.......................................................................................................... 14

5.3 The operating elements and their function - VT1000 S ........................................................ 15

5.4 Adjusting the amplitude............................................................................................................. 19

5.5 Working with the VT1000 S on a daily basis........................................................................... 20

5.6 Routine daily maintenance and switching off the instrument - VT1000 S......................... 23

6. Malfunctions: Meaning and Troubleshooting.........................................................24

7. Cleaning and Maintenance ........................................................................................29

7.1 Cleaning the instrument............................................................................................................. 29

7.2 Changing the fuse....................................................................................................................... 30

8. Ordering Information: Spare Parts, Accessory, Consumables.............................31

8.1 Foot switch................................................................................................................................... 32

8.2 Buffer tray.................................................................................................................................... 32

8.2.1 Double-walled buffer tray S...................................................................................................... 32

8.3 Magnifier, LED illumination....................................................................................................... 33

8.4 Julabo recirculating cooler/chiller FL300............................................................................... 34

9. Warranty and Service..................................................................................................35

10. Decontamination Confirmation..................................................................................36

Leica VT1000 S - Vibrating-blade microtome

Symbols used in this manual and their meaning

1. Important Information

(5)

(Fig. 5)

Numbers in parentheses refer to item num-

bers in illustrations or to the illustrations

themselves.

Useful tips,

i.e. important information for the user,

appear in gray boxes and are marked by

an .

Dangers, warnings and cautions

appear in a gray box and are marked by

a warning triangle .

Environmental protection symbol of the

China RoHS directive.

The number in the symbol indicates the

"Environment-friendly Use Period" of the

product in years.

The symbol is used if a substance

restricted in China is used in excess of

the maximum permitted limit.

The CE labeling shows that the product

corresponds to one or more applicable

European directives.

Symbol for labeling electrical and elec-

tronic equipment in accordance with

Section 7 of the German Electrical and

Electronic Equipment Act (ElektroG).

ElektroG is the law on the bringing into

circulation, return and environmentally

compatible disposal of electrical and

electronic equipment.

Caution! Follow the accompanying

documentation!

Instrument model:

All information provided in these Instructions for

Use applies only to the instrument type indicated

on the title page. A nameplate indicating the

instrument serial number is attached to the rear

side of the instrument.

Information:

When making inquiries, please specify correctly:

• Instrument model • Serial number

Observe the Instructions for Use

Order No.

Serial number

Date of Manufacture

Manufacturer

The Regulatory Compliance Mark (RCM)

indicates a device’s compliance with

applicable ACMA technical standards of

New Zealand and Australia - that is, for

telecommunications, radio communica-

tions, EMC and EME.

4Instructions for Use, V 2.3 RevK – 09/2018

Qualification of personnel

The Leica VT1000 S should be operated by trained laboratory personnel only.

All laboratory personnel designated to operate this instrument must read these Instructions for

Use carefully and must be familiar with all technical features of the instrument before attempt-

ing to operate it.

Intended use/improper use

The VT1000 S is used for sectioning in the fields of medicine, biology and industry, and is espe-

cially designed for sectioning fixed or unfixed fresh tissue in a buffer solution.

The VT1000 S may be used for research purposes only. Sections made using the VT1000 S

must NOT be used for diagnostics!

The instrument must be used exclusively according to the instructions contained in these

Instructions for Use.

Any other use of the instrument is considered improper.

1. Important Information

Leica VT1000 S - Vibrating-blade microtome

2. Safety

2.1 Safety notes

These Instructions for Use includes important

information related to the operating safety and

maintenance of the instrument.

The Operating Manual is an important part of

the product, and must be read carefully prior to

startup and use and must always be kept near

the instrument.

This instrument has been built and tested in

accordance with the safety requirements for

electrical equipment for measurement, control,

and laboratory use.

To maintain this condition and ensure safe opera-

tion, the user must observe all notes and warnings

contained in these Instructions for Use.

The current EC Declarations of Conformity can be

found on the Internet:

www.LeicaBiosystems.com

2.2 Warnings

The safety devices installed in this instrument by the manufacturer only constitute the basis

for accident prevention. Operating the instrument safely is, above all, the responsibility of the

owner, as well as the designated personnel who operate, service or clean the instrument.

To ensure trouble-free operation of the instrument, make sure to comply with the following

instructions and warnings.

These Instructions for Use must be ap-

propriately supplemented as required

by the existing regulations on accident

prevention and environmental safety in

the operator‘s country.

The safety and caution notes in this chapter must be observed at all times.

Be sure to read these notes even if you are already familiar with the operation and use of

other Leica products.

The protective devices located on the instrument and the accessories must not be

removed or modified. The instrument must only be opened and repaired by service techni-

cians authorized by Leica.

6Instructions for Use, V 2.3 RevK – 09/2018

2. Safety

2.3 Safety instructions for handling the instrument

Proper handling

Be sure to handle knives and blades very cau-

tiously!

Never touch the cutting edge of knives and

blades!

Do not leave knives, blades and bladed knife

holders unprotected.

All appropriate safety precautions must be met

to avoid the risk of infection.

Protective clothing according to safety regula-

tions for "Working with harmful substances"

(Safety mask, gloves, protective clothing) must

be worn!

Cover the magnifier during work breaks as it may

act as a burning glass when not covered!

In case of emergency, press the red EMERGENCY

STOP switch (at the right side of the instrument).

To release the switch, turn it in the direction of

the arrow.

The instrument may be opened by authorized

service personnel only.

Before removing the cover, ensure that the instru-

ment is unplugged.

Warning: Avoid touching live parts

under any circumstances!

Danger

Caution: Extremely sharp blades pose

risk of injury when touched!

Caution: Fresh tissue poses risk of

infection!

Caution: When not in use, cover magni-

fier with corresponding lid to avoid risk

of fire.

Leica VT1000 S - Vibrating-blade microtome

3. Instrument Characteristics

3.1 Technical Data

General data:

Sectioning frequency (± 10 %).............................................................................................................0 - 100 Hz

Amplitude.................................................................................... adjustable in 5 steps: 0.2; 0.4; 0.6; 0.8; 1 mm

Sectioning speed (± 10 %) ........................................................................................................ 0.025 - 2.5 mm/s

Return stroke speed (± 10 %) ...................................................................................................................5 mm/s

Total vertical specimen stroke............................................................................................15 mm (motorized)

Sectioning range ............................................................................................................ 1 - 40 mm (adjustable)

Specimen retraction .................................................................0 - 999 µm (adjustable; can be deactivated)

Maximum specimen size:

with standard knife holder.......................................................................................................... 33 x 40 mm

Specimen orientation.....................................................................................................................................330°

Section thickness selection......................................................................................1 - 999 µm, in 1-µm steps

Magnifier, assy. (standard accessory of the configured instrument)............................ 2 x magnification

Ambient conditions:

Operating temperature range....................................................................................... min. 5 °C to max. 40 °C

Relative humidity .................................................................................................................................. max. 80 %

Height: .................................................................................................................. up to 2000 m above sea level

Electrical data:

Rated voltage range (± 10 %): ........................................................................................................ 100 V - 240 V

Nominal frequency (± 10 %): ................................................................................................................50 - 60 Hz

Power draw .................................................................................................................................................. 50 VA

Mains fuse .................................................................................................................................................T 1.25 A

Pollution degree....................................................................................................................................................2

Overvoltage installation category..................................................................................................................... II

Overload protection ........................................................................................................................................ yes

Internal current limiter of electronics.......................................................................................................... yes

Dimensions:

L x W x H...............................................................................................................480 mm x 360 mm x 200 mm

Height with magnifier support................................................................................................................ 285 mm

Weight:

(without magnifier support) ........................................................................................................................ 17 kg

(magnifier support only) ................................................................................................................................ 2 kg

(total)............................................................................................................................................................... 19 kg

8Instructions for Use, V 2.3 RevK – 09/2018

3. Instrument characteristics

3.2 General overview - VT1000 S

Buffer tray S

EMERGENCY STOP

switch (not visible here)

Fixture for

magnifier

support

Module LED Hi-Power spots, 2-arm

(optional)

Magnifier

Module Hi-Power spot, LED 1000

(optional)

Knife holder

Control panel

Magnifier support

Cutting head

Fig. 2

Otros manuales para VT1000 S

Tabla de contenidos

Otros manuales de Equipo de laboratorio de Leica

Leica

Leica HistoCore AUTOCUT Manual de usuario

Leica

Leica PAULA Manual de usuario

Leica

Leica EM TIC 3X Manual de usuario

Leica

Leica CaptiView Manual de usuario

Leica

Leica RM2235 Manual de usuario

Leica

Leica ThermoBrite S500 Manual de usuario

Leica

Leica AutoStainerXL Manual de usuario

Leica

Leica BOND-PRIME Manual de usuario

Leica

Leica FL400 Manual de usuario

Leica

Leica Z6 APO Manual de usuario

Leica

Leica Ivesta 3 Series Manual de usuario

Leica

Leica BLK3D Manual de usuario

Leica

Leica Flexacam i5 Manual de usuario

Leica

Leica VT1200 Manual de usuario

Leica

Leica HistoCore Arcadia C Manual de usuario

Leica

Leica CM1510 Manual de usuario

Leica

Leica HistoCore Arcadia H Manual de usuario

Leica

Leica CM3050 S Manual de usuario

Leica

Leica RM2125RTS Manual de usuario

Leica

Leica Envisu C Series Instrucciones de uso

Leica

Leica CM1520 Manual de usuario

Leica

Leica CM1950 Manual de usuario

Leica

Leica TCS SP8 X Manual de usuario

Leica

Leica Aperio GT 450 Manual de usuario

Manuales populares de Equipo de laboratorio de otras marcas

Qiagen

Qiagen DML 3000 Manual de usuario

Queensgate

Queensgate NANOSCAN OP400 Manual de programación

Conviron

Conviron GEN1000-GE Manual de usuario

Parr Instrument

Parr Instrument 4560 Manual de instrucciones

IKA

IKA IC control Manual de usuario

Integra

Integra DOSE IT Manual de usuario

Agilent Technologies

Agilent Technologies 5800 ICP-OES Manual de usuario

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manual de usuario

NI PXI-5422 Manual

Collomix

Collomix Aqix Manual de usuario

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manual de usuario

Ocean Insight

Ocean Insight FLAME-NIR+ Manual de usuario

Parker

Parker ALIGN-MG-NA Manual de usuario

BD 644787 Manual de usuario

DENTAURUM

DENTAURUM Compact Megaplus Manual de usuario

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manual de usuario

VWR

VWR SAS Super IAQ Manual de usuario

illumina

illumina MiSeqDx Manual de usuario