Labnet Prism Manual de usuario

Prism™ Microcentrifuge

Instruction Manual

Catalog Numbers:

C2500, C2500-230V

This manual is available in additional languages at www.labnetlink.com.

Table of Contents

1.0 Introduction ...................................................................................1

2.0 Safety Information .............................................................................1

2.1 Operation Safety Precautions................................................................1

3.0 Specifications ..................................................................................2

4.0 Package Contents ..............................................................................2

5.0 Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2

5.1 Unpacking the Centrifuge ...................................................................2

5.2 Required Space .............................................................................2

5.3 Installing the Centrifuge ....................................................................2

6.0 Installation of Rotors and Rotor Maintenance....................................................3

6.1 Rotors and Accessories ......................................................................3

6.2 Rotor Maintenance .........................................................................4

6.3 Removing and Installing the Angle Rotor.....................................................4

6.4 Loading the Rotor...........................................................................4

6.5 Overloading Rotors .........................................................................4

7.0 Operation......................................................................................5

7.1 Attaching Rotor Lid .........................................................................5

7.2 Closing the Lid..............................................................................5

7.3 Lid Release .................................................................................5

7.4 Lid Lock ....................................................................................5

7.5 Speed Selection.............................................................................5

7.6 Selection of Operating Time, Momentary Operation, Start/Stop ...............................6

8.0 Service and Maintenance .......................................................................6

8.1 Centrifuge Service ..........................................................................6

8.2 Cleaning ...................................................................................6

8.3 Replacing Fuses.............................................................................6

9.0 Troubleshooting Guide .........................................................................7

10.0 Determination of G-values......................................................................8

11.0 Limited Warranty...............................................................................9

12.0 Equipment Disposal ............................................................................9

Instruction Manual | 1

1.0 Introduction

The Prism™ is a small benchtop centrifuge designed for separation of various research samples. The

motor is brushless and requires no routine maintenance. The Prism Microcentrifuge is supplied with a

24 x 1.5/2.0 mL rotor for micro sample tubes. Adapters are available for tubes smaller than 1.5 mL. The

Prism reaches speeds of up to 15,000 rpm/21,200 x g.

2.0 Safety Information

Before using the Prism Microcentrifuge for the first time, please read this entire manual carefully.

If the equipment is used in a manner not specified by the manufacturer, the protection provided by

the equipment may be impaired.

2.1 Operation Safety Precautions

Never use the centrifuge in any manner not specified in these instructions.

Never operate the centrifuge without a rotor properly attached to the shaft.

Never tighten rotor nut by hand only.

Never fill tubes while they are in the rotor. Liquid spillage may harm unit.

Never put hands in the rotor area unless the rotor is completely stopped.

Never move the centrifuge while the rotor is spinning.

Never use solvents or flammables near this or other electrical equipment.

Never centrifuge flammable, explosive or corrosive materials.

Never centrifuge hazardous materials outside of a hood or proper containment facility.

Always load the rotor symmetrically. Each tube should be counter balanced by another tube of the

same type and weight.

Always locate the centrifuge within easy access to an electrical outlet.

Always use only microcentrifuge tubes made from plastic and designed to withstand centrifugal forces

of at least 21,200 x g.

Always use a wrench to tighten the rotor nut.

Do not operate the centrifuge if any of the following conditions exist:

The centrifuge has not been installed properly.

The centrifuge is partially dismantled.

Service has been attempted by unauthorized or unqualified personnel.

The rotor has not been installed securely on the motor shaft.

Rotors and accessories not belonging to the standard range are being used without permission being

obtained from the manufacturer to use such rotors and/or accessories in the centrifuge.

Exception: Microcentrifuge tubes made of plastic, normally available in the laboratory.

The centrifuge is located in an explosive atmosphere.

Materials to be centrifuged are combustible and/or explosive.

Materials to be centrifuged are chemically reactive.

The rotor load is not properly balanced.

The rotor nut was not tightened with a wrench.

2| LABNET PRISM MICROCENTRIFUGE

3.0 Specications

Dimensions (W x D x H) 10.25 x 13.8 x 7.48 in.

(26 x 35 x 19 cm)

Maximum Speed 15,000 rpm

Maximum RCF 21,200 x g

Maximum Volume 24 x 2.2 mL

Timer 0.5 to 99 minutes or continuous

Admissible Sample Density 1.2 kg/dm3

Electrical/Fuse Rating 120V, 50-60Hz, 1.9A, 5AT

230V, 50-60Hz, 1.1A, 2.5AT

Operating Conditions 0°C to 40°C / ≤80%RH

4.0 Package Contents

Description Quantity

Prism™ Microcentrifuge 1 each

Standard angle rotor (Cat. No. 24D-RTR) 1 each

Rotor removal tool 1 each

Instruction manual 1 each

Warranty card 1 each

Power cord 1 each

5.0 Installation

The centrifuge should be installed on a rigid, even surface such as a stable laboratory bench,

countertop, etc. To guarantee sufficient ventilation, ensure that the centrifuge has at least 15 cm

(6 inches) of free space on all sides, including the rear. The centrifuge should not be located in areas

subject to excessive heat such as in direct sunlight or near radiators or the exhaust of a compressor, as

a buildup of heat may occur within the chamber.

Before operating the centrifuge, check that the power source (electrical outlet on the wall)

corresponds to that on the manufacturer’s rating label, then connect the power cord to the centrifuge

and the power source.

Instruction Manual | 3

6.0 Installation of Rotors and Rotor Maintenance

6.1 Rotors and Accessories

The following accessories are included or available for the Prism™ Microcentrifuge:

Angle rotor for 24 x 1.5 ml tubes (included)

Cat. No. 24D-RTR

Tube Measurement 1.5 mL / 2.0 mL (10 x 40 mm)

Maximum Speed 15,000 rpm

Centrifuging Radius 8.4 cm

Maximum RCF (G-value) 21,200 x g

Rotor Wrench (included)

Cat. No. AM190

Rotor Lid (included)

Cat. No. C2400-RC1

Rotor Securing Screw (included)

Cat. No. AR110

Strip Spin Adapter

Cat. No. C2400-SS

Tubes Accepted 0.2 mL tubes or 8 x 0.2 mL strips

Maximum Speed 15,000 rpm

Centrifuge Radius Range 4.32 cm to 5.33 cm

RCF (G-value) Range 10,874 x g to 13,405 x g

Adapter for 0.5 mL tubes (pk./6)

Cat. No. C-1205

Tube Measurement 8 x 30 mm

Maximum Speed 15,000 rpm

Centrifuging Radius 7.53 cm

RCF (G-value) 18,942 x g

Adapter for 0.4 mL tubes (pk./6)

Cat. No. C-1206

Tube Measurement 6 x 47 mm

Maximum Speed 15,000 rpm

Centrifuging Radius 8.4 cm

RCF (G-value) 21,200 x g

Adapter for 0.2 mL tubes (pk./6)

Cat. No. C-1222

Tube Measurement 6 x 21 mm

Maximum Speed 15,000 rpm

Centrifuging Radius 7.03 cm

RCF (G-value) 17,684 x g

4| LABNET PRISM MICROCENTRIFUGE

6.2 Rotor Maintenance

The rotor should be cleaned thoroughly after each use. Thorough cleaning must be performed when

spinning samples containing phenol or phenol chloroform. Periodically inspect the rotor for dents,

dings, scratches, discoloration and cracks. If any damage to the rotor is found, discontinue use of the

rotor immediately and replace.

6.3 Removing and Installing the Angle Rotor

The Prism™ Microcentrifuge comes complete with a standard 24-place rotor installed. To remove

the rotor for cleaning, remove the rotor securing screw from the motor shaft by turning the screw

counterclockwise, using the rotor wrench. Lift the rotor directly upward in a straight vertical motion.

Figure 1. Loading the rotor to achieve balance

To replace rotor, first make sure the motor shaft and rotor mounting hole are clean. Place the rotor on

the motor shaft. Reinstall the rotor securing screw on the motor shaft by turning it clockwise. Hold the

rotor with one hand and tighten the rotor securing screw, using the rotor wrench.

6.4 Loading the Rotor

Tubes to be loaded should be filled equally by eye. The difference in the weight between the tubes

should not exceed 0.1 gram. Tubes should always be loaded so that there is equal spacing between all

tubes. One or two additional loaded tubes may need to be added to achieve this. Refer to Figure 1. to see

one typical balancing scheme.

6.5 Overloading Rotors

The maximum load of the rotor and maximum speed has been established by the manufacturer. Do

not attempt to exceed these values. The maximum speed of the rotor has been established for liquids

having a homogeneous density of 1.2g/ml or less. In order to centrifuge liquids with a higher density

it is necessary to reduce the speed. Failure to reduce the speed may result in damage to the rotor and

centrifuge. The revised maximum speed can be calculated with the following formula:

1.2

higher density value

Reduced speed (n

red) = × max speed (nmax)

1.2

1.7

(nred) = × 15,000 = 12,602 rpm

Example: Where the density of the liquid is 1.7, the new maximum speed would be calculated as follows:

1.2

higher density value

Reduced speed (nred) = × max speed (nmax)

1.2

1.7

(nred) = × 15,000 = 12,602 rpm

If in doubt concerning maximum speeds, please contact the manufacturer for assistance.

Instruction Manual | 5

7.0 Operation

ATTENTION: Never attempt to operate the centrifuge with rotors or adapters that show signs of

corrosion or mechanical damage. Never centrifuge strongly corrosive materials that may damage the

rotors, accessories, or bowl of the unit.

7.1 Attaching Rotor Lid

After the rotor has been properly secured and loaded, attach the rotor lid to the rotor. Always use the

rotor lid for safety and to allow the rotor to reach proper speed. Make sure that the rotor lid snaps

securely into place, by pressing down on center catch.

7.2 Closing the Lid

Close the centrifuge lid. The Prism™ Microcentrifuge has a lid lock that activates only when a run is started.

7.3 Lid Release

The lid will remain locked during a centrifuge run. Once the run has been completed and the rotor has

come to a stop, a beep will indicate the end of a run, and the lid will unlock automatically.

WARNING: Do not attempt to open the lid of any centrifuge until the rotor has come to a complete stop.

In the event of a power failure or malfunction, it may be necessary to open the lid manually.

1. Disconnect the power cord from the wall socket.

2. Remove the plastic plug, located on the left side of the unit.

3. Pull the wire (attached to the plug) to open the lid lock manually.

7.4 Lid Lock

The centrifuge can be started only with the lid securely closed. When a run is started, the lid lock

automatically activates. Do not attempt to open the lid during a centrifuge run. At the end of the

run, the lid will automatically unlock. Never attempt to override the lid lock mechanism. Doing so is

dangerous and could damage the centrifuge.

7.5 Speed Selection

Figure 2. Prism Microcentrifuge control panel layout

The speed (rpm or g-force) can be selected from 500 to 15,000 rpm in 100 rpm increments or from

100 to 21,200 x g with the control knob. The speed is selected by pressing the RPM or RCF button. The

speed signal will begin to blink. Then, turn the control knob to increase or decrease the value.

6| LABNET PRISM MICROCENTRIFUGE

7.6 Selection of Operating Time, Momentary Operation, Start/Stop

Operating time can be selected from 0.5 min to 99 min by pressing the TIME button and adjusting with

the control knob. The time can be set in 0.5 minute increments from 0 to 10 minutes and in 1 minute

increments from 10 to 99 minutes. After 99 minutes, the display shows “--” which indicates continuous

run. In this mode, the centrifuge will run until manually stopped. To start a run, press the control knob.

When the preselected time expires, the centrifuge will stop automatically. To stop the centrifuge prior

to the expiration of set time, press the control knob. The centrifuge may be operated for a short run by

pressing and holding the control knob. The centrifuge will continue to run as long as the control knob is

depressed and the time, in seconds will count up on the time display.

8.0 Service and Maintenance

8.1 Centrifuge Service

The brushless motor in the Prism™ Microcentrifuge requires no routine maintenance. Any required

service should be performed by authorized, qualified personnel only. Repairs performed by unauthorized

personnel may void the warranty.

8.2 Cleaning

Always keep the centrifuge housing, rotor chamber, rotor and rotor accessories clean. All parts should be

wiped down periodically with a soft cloth. For more thorough cleaning, use a neutral cleaning agent (pH

between 6 and 8) applied with a soft cloth. Excessive amounts of liquid should be avoided. Liquid should

not come into contact with the motor. After cleaning, ensure that all parts are dried thoroughly by hand

or in a warm air cabinet (maximum temperature 50°C).

The rotor should be cleaned after each use. When spinning samples containing phenol or phenol

chloroform, the rotor should be cleaned immediately after use.

Should a spill of infectious materials occur within the rotor or chamber, the unit should be disinfected.

This should be performed by qualified personnel with proper protective equipment.

8.3 Replacing Fuses

Check the fuse when it is recommended in the Troubleshooting Guide located in this manual. The fuse

holder is located in the power inlet on the rear of the unit. Disconnect the power cord from the power

inlet. Open the fuse holder drawer by inserting a small screwdriver under the tab and prying it open.

Remove the innermost (operative) fuse from its retaining tabs and replace the fuse if necessary. A spare

fuse is located in the outermost chamber of the fuse drawer. Replace only with a fuse of exactly the

same value as the original. Fuse type may be found in the Technical data section of this manual.

Instruction Manual | 7

9.0 Troubleshooting Guide

Problem Possible Cause Solution

Centrifuge will not start No power supply Check that power is being supplied to

the outlet

Check that the power cord is plugged

into both the wall

outlet and the back of the centrifuge

Check that power cord is not damaged

Blown fuse Check fuse and replace if necessary

Lid lock will not release Defective lid lock Open manually and have unit serviced

No power from PC board Call for service

Lid lock is jammed Call for service

Centrifuge is not receiving

power

See “Centrifuge will not start”

Centrifuge cannot be

started, although power

is on

Lid not closed correctly Close lid correctly

No speed or time has been

selected

Set speed and/or time

bAL: Error Message

Indicates imbalance

Tubes not inserted

symmetrically in rotor holes

Load tubes symmetrically (see Section 3.4

on Loading the rotor)

Sample liquid in tubes not

balanced

Make sure the same volume of liquid is in

each tube

Defective or improperly

adjusted balance sensor

Call service

Lid: (Error message) Lid

not closed

Lid not closed completely Close lid

Lid lock or sensor defective Call service

Other Error Messages Er

on Display

–Press time or speed knob to clear error

Call service

Should you have a question about the operation of the Labnet Prism™ Microcentrifuge or if service is

required, contact Customer Service. Do not send in a unit for service without first calling to obtain a

repair authorization number. Should the unit require return for service, it should be properly packed to

avoid damage. Any damage resulting from improper packaging shall be the responsibility of the user.

8| LABNET PRISM MICROCENTRIFUGE

10.0 Determination of G-values

The centrifuging radius of the 1.5/2.0 mL rotor is 8.23 cm. The Prism™ Microcentrifuge has an automatic

g-force conversion program, so g-values are automatically calculated and can be displayed on the control

panel, for this centrifuging radius. If adapters or smaller tubes are used, the centrifuging radius changes

as does the g force. The chart on the next page can be used to manually determine g-values for any

centrifuging radius.

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