Agilent Technologies 2100 Bioanalyzer System Instrucciones de funcionamiento

Agilent Technologies

Agilent 2100 Bioanalyzer

System

Maintenance and Troubleshooting

Guide

Maintenance and Troubleshooting

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or perfor-

mance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a

license and may be used or copied only in

accordance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of

a U.S. Government prime contract or sub-

contract, Software is delivered and licensed

as “Commercial computer software” as

defined in DFAR 252.227-7014 (June 1995),

or as a “commercial item” as defined in

FAR 2.101(a) or as “Restricted computer

software” as defined in FAR 52.227-19

(June 1987) or any equivalent agency regu-

lation or contract clause. Use, duplication

or disclosure of Software is subject to Agi-

lent Technologies’ standard commercial

license terms, and non-DOD Departments

and Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully

understood and met.

Notices

2017

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from Agi-

lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G2946-90003 Rev. B

Edition

02/2017

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

For Research Use Only

Not for use in diagnostic procedures

Maintenance and Troubleshooting 3

In this Book

This manual provides maintenance and troubleshooting information for the

Agilent 2100 Bioanalyzer system. It includes essential measurement practices,

troubleshooting hints for hardware, software and applications, maintenance

procedures and a list of spare parts and accessories.

This manual is based on the 2100 Expert Software revision B.02.08. Other

software revisions may have an impact on results.

4 Maintenance and Troubleshooting

Contents

1 Essential Measurement Practices 7

Overview 8

Tools and Handling 9

Chip Priming Station 10

Reagents and Reagent Mixes 11

Samples 12

Chips 13

Agilent 2100 Bioanalyzer System 14

2 Troubleshooting the Instrument Communication 15

Verify the Instrument Communication 16

Troubleshooting Communication Issues 18

Changing COM Port Settings 20

USB to Serial Adapter 21

3 Troubleshooting the 2100 Expert Software 25

Run Installation Qualification Test 26

4 Hardware Diagnostics 29

Overview 30

Diagnostic Test Procedure 32

5 Troubleshooting the DNA Application 35

Overview 36

Symptoms (DNA) 37

Symptoms (High Sensitivity DNA) 62

6 Troubleshooting the RNA Application 67

Overview 68

Symptoms (RNA) 69

Maintenance and Troubleshooting 5

Contents

7 Troubleshooting the Protein Application 87

Overview 88

Symptoms (Protein) 89

Symptoms (High Sensitivity Protein) 112

8 Maintenance of the Electrode Cartridge 115

Overview 116

DNA and Protein Assays 117

RNA Nano Assay 119

RNA Pico or Small RNA Assay 123

How to Clean the Pin Set of the Electrode Cartridge 126

9 Maintenance of the Chip Priming Station 131

Overview 132

Replacing the Syringe 133

Cleaning the Syringe Adapter 134

Replacing the Syringe Adapter 136

Replacing the Gasket 137

Checking the Chip Priming Station for Proper Performance - Seal Test 139

10 Maintenance of the Agilent 2100 Bioanalyzer instrument 141

Overview 142

Cleaning the Lens 143

Changing the Fuses 144

11 Maintenance of the Vortexer 149

Changing the Adapter 150

12 Spare Parts and Accessories 151

Overview 152

6 Maintenance and Troubleshooting

Contents

Maintenance and Troubleshooting

Agilent Technologies

Essential Measurement Practices

Overview 8

Tools and Handling 9

Chip Priming Station 10

Reagents and Reagent Mixes 11

Gel and Gel-Dye Mix 11

Samples 12

Chips 13

Agilent 2100 Bioanalyzer System 14

8 Maintenance and Troubleshooting

Essential Measurement Practices

Overview

This section lists all user relevant hints on handling tools, chips, reagents and

the Agilent 2100 Bioanalyzer system. For the latest information on

assay-related hints, go to the Agilent web site at:

www.agilent.com/genomics/bioanalyzer

Maintenance and Troubleshooting 9

Essential Measurement Practices

Tools and Handling

•Always follow the GLP-rules established in the laboratory.

•Always wear gloves when handling chips to prevent contamination.

•When pipetting sample, use non-filter pipette tips that are of adequate size.

Pipette tips that are too large will lead to poor quantitation accuracy.

•Change pipette tips between steps to avoid cross-contamination.

•Always insert the pipette tip to the bottom of the well when dispensing

liquid. Placing the tip at the edge of the well leads to bubbles and poor

results. Holding the pipette at a slight angle will ensure proper dispensing

of the liquid.

•Use a new syringe and electrode cleaner with each new kit.

10 Maintenance and Troubleshooting

Essential Measurement Practices

Chip Priming Station

•Refer to the appropriate Kit Guide for the correct position of the syringe

clip and base plate.

•Replace the syringe with each new kit.

•Check the performance of the chip priming station by applying the seal test

on a monthly basis. For details see “Maintenance of the Chip Priming

Station” on page 131. If necessary, replace the gasket and/or adapter.

Otros manuales para 2100 Bioanalyzer System

Tabla de contenidos

Otros manuales de Instrumento de medición de Agilent Technologies

Agilent Technologies

Agilent Technologies N4373A Manual de usuario

Agilent Technologies

Agilent Technologies B1500A Guía de instalación

Agilent Technologies

Agilent Technologies 85097B Manual de usuario

Agilent Technologies

Agilent Technologies 4338B Manual de usuario

Agilent Technologies

Agilent Technologies E5070B ENA Series Manual de usuario

Agilent Technologies

Agilent Technologies 6813B Manual de usuario

Agilent Technologies

Agilent Technologies 8960 Manual de usuario

Agilent Technologies

Agilent Technologies ESA-E Series Manual del propietario

Agilent Technologies

Agilent Technologies E5505A Manual de usuario

Agilent Technologies

Agilent Technologies E4411-60019 Manual de instalación y operación

Agilent Technologies

Agilent Technologies 85052A Instrucciones de funcionamiento

Agilent Technologies

Agilent Technologies 86038B Manual de usuario

Agilent Technologies

Agilent Technologies B1500A Guía de instalación

Agilent Technologies

Agilent Technologies Seahorse XFe Manual de usuario

Agilent Technologies

Agilent Technologies 1290 Infinity II 2D-LC Solution Manual de usuario

Agilent Technologies

Agilent Technologies E8362C Manual de usuario

Agilent Technologies

Agilent Technologies E5071C ENA Series Manual de usuario

Agilent Technologies

Agilent Technologies U1731C Manual de usuario

Agilent Technologies

Agilent Technologies PSA Series Manual de usuario

Agilent Technologies

Agilent Technologies B1500A Manual de usuario

Agilent Technologies

Agilent Technologies N1913a Manual de usuario

Agilent Technologies

Agilent Technologies 8510C Manual de usuario

Agilent Technologies

Agilent Technologies EMC Series Manual

Agilent Technologies

Agilent Technologies E4991A Manual de usuario

Manuales populares de Instrumento de medición de otras marcas

Suaoki

Suaoki D40 Manual de usuario

Elster

Elster BK-G1.6 Manual de usuario

Endress+Hauser

Endress+Hauser Deltabar FMD71 Manual

Duratool

Duratool D03128 Manual de usuario

Vega

Vega VEGAPULS 65 Manual de usuario

Tonghui

Tonghui TH2618B Manual de usuario

Tenmars

Tenmars TM-801 Manual de usuario

MSR

MSR 145 Manual de usuario

TPS

TPS WP-82 Manual de usuario

ELMETRON

ELMETRON CP-511 Manual de usuario

PCB Piezotronics

PCB Piezotronics 003C30 Manual de usuario

Tenma

Tenma 72-10465 Manual de usuario

Endress+Hauser

Endress+Hauser Proline Promag 50 Especificaciones técnicas

Siemens

Siemens SITRANS F Coriolis FCT030 Manual de lista de piezas

KLINGER

KLINGER CMF V Series Manual de usuario

EXFO

EXFO FTB-2 Manual de operación y mantenimiento

Keysight

Keysight M8290A Manual de usuario

ADTEK

ADTEK MW-5 Manual de usuario