Agilent Technologies PL-GPC 220 Manual de usuario

Agilent Technologies

Agilent PL-GPC 220

Integrated HT-GPC/SEC

System

User Manual

PL-GPC 220 User Manual

Notices

No part of this manual may be reproduced

in any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G7820-90220

Edition

09/2012

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

This product may be used as a com-

ponent of an in vitro diagnostic sys-

tem if the system is registered with

the appropriate authorities and com-

plies with the relevant regulations.

Otherwise, it is intended only for gen-

eral laboratory use.

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or perfor-

mance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

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and may be used or copied only in accor-

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Restricted Rights Legend

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tract, Software is delivered and licensed as

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or as a “commercial item” as defined in FAR

2.101(a) or as “Restricted computer soft-

ware” as defined in FAR 52.227-19 (June

1987) or any equivalent agency regulation

or contract clause. Use, duplication or dis-

closure of Software is subject to Agilent

Technologies’ standard commercial license

terms, and non-DOD Departments and

Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully under-

stood and met.

PL-GPC 220 User Manual 3

In This Guide...

This manual contains information on the Agilent PL-GPC 220 Integrated

HT-GPC/SEC System (G7820A)

1 Introduction to PL-GPC 220

This chapter gives an instrument overview and an introduction to the function

of the PL-GPC 220.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical

and performance specifications.

3 Unpacking the Instrument

This chapter gives information on how to unpack your PL-GPC 220.

4 Using the Module

This chapter provides information on how to set up the instrument for an

analysis and explains the basic settings.

5 Troubleshooting and Diagnostics

This chapter gives an overview about the troubleshooting and diagnostic

features.

6 Error Information

This chapter describes the meaning of error messages, and provides

information on probable causes and suggested actions how to recover from

error conditions.

7 Maintenance

This chapter provides general information on maintenance of the PL-GPC 220.

4PL-GPC 220 User Manual

In This Guide...

8 Parts for Maintenance

This chapter provides information on parts for maintenance.

9 Appendix

This chapter provides addition information on safety, legal and web.

PL-GPC 220 User Manual 5

Contents

1 Introduction to PL-GPC 220 7

Introduction 8

System Description and General Operation 9

2 Site Requirements and Specifications 19

Site Requirements 20

Physical Specifications 23

Performance Specifications 24

3 Unpacking the Instrument 25

Unpacking the Instrument 26

4 Using the Module 29

The Graphical User Interface 30

Setting Up the PL-GPC 220 45

Running Samples on the PL-GPC 220 53

5 Troubleshooting and Diagnostics 55

Diagnostics 56

General Troubleshooting Guide 58

6 Error Information 63

Errors 64

7 Maintenance 69

Introduction 70

Warnings and Cautions 71

Cleaning 73

Decontamination 74

Testing Overtemperature Protection Systems 75

Testing Vapor Sensors 81

6PL-GPC 220 User Manual

Contents

Testing Solvent Level Sensors 86

Testing Oven Door Interlock 88

Laser Interlock Switch 89

Degasser Test 90

Back Pressure Regulator (BPR) 91

Differential Refractive Index Detector 93

Autosampler 97

8 Parts for Maintenance 103

Parts List 104

Pump Spares 105

9 Appendix 107

General Safety Information 108

The Waste Electrical and Electronic Equipment Directive 113

Radio Interference 114

Sound Emission 115

Agilent Technologies on Internet 116

PL-GPC 220 User Manual

Agilent Technologies

Introduction to PL-GPC 220

Introduction 8

System Description and General Operation 9

Instrument Overview 9

Solvent Module 11

Column Oven 13

PL-GPC 220 Autosampler 15

Injection Sequence 17

This chapter gives an instrument overview and an introduction to the function

of the PL-GPC 220.

8PL-GPC 220 User Manual

1Introduction to PL-GPC 220

Introduction

The Agilent PL-GPC 220 Integrated HT GPC/SEC System is a fully automated

chromatograph designed specifically for Gel Permeation Chromatography

(GPC) / Size Exclusion Chromatography (SEC). The instrument features a

40-position carousel, high sensitivity refractive index detector and a reliable

isocratic solvent delivery system housed uniquely in a temperature controlled

enclosure. The open architecture of the column oven enables multi-detection

options both inside and outside of the oven. Although the system was designed

for prolonged high temperature operation, the system delivers an equal level

of performance across the whole of its temperature operating range (30 –

220 °C).

Figure 1 Front View of the Agilent PL-GPC 220 System

PL-GPC 220 User Manual 9

Introduction to PL-GPC 220 1

System Description and General Operation

Instrument Overview

Figure 2 Overview (modules)

(

)

1 Control drawer

2Autosampler

3 Main oven module

4 Solvent delivery module

5Pump

6 Solvent degasser

10 PL-GPC 220 User Manual

1Introduction to PL-GPC 220

System Description and General Operation

Figure 3 Overview (display and control elements)

(

)

1 Power switches

2 Oven over-temperature display and control

3 Autosampler over-temperature display and control

4 Temperature and RI display

5 Oven and laser safety interlock switches

6 Status indicator

7Powerindicators

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