Agilent Technologies G7166A Manual de usuario

Agilent Technologies

Agilent 1260 Infinity II

Preparative Valve-Based

Fraction Collector

User Manual

Preparative Valve-Based Fraction Collector

Notices

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from Agi-

lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G7166-90000 Rev. B

Edition

03/2018

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or perfor-

mance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

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document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a

license and may be used or copied only in

accordance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of

a U.S. Government prime contract or sub-

contract, Software is delivered and licensed

as “Commercial computer software” as

defined in DFAR 252.227-7014 (June 1995),

or as a “commercial item” as defined in

FAR 2.101(a) or as “Restricted computer

software” as defined in FAR 52.227-19

(June 1987) or any equivalent agency regu-

lation or contract clause. Use, duplication

or disclosure of Software is subject to Agi-

lent Technologies’ standard commercial

license terms, and non-DOD Departments

and Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully

understood and met.

Preparative Valve-Based Fraction Collector 3

In This Guide

This manual contains technical reference information about the Agilent 1260

Infinity II Preparative Valve-Based Fraction Collector.

1 Introduction to the Valve-Based Fraction Collector

This chapter gives an introduction to the module, instrument overview and

internal connectors.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical

and performance specifications.

3 Installing the Preparative Valve-based Fraction Collector

This chapter gives information about the installation of the module.

4 Using the Preparative Valve-Based Fraction Collector

This chapter explains the essential operational parameters of the module.

5 Preparing the Preparative Valve-Based Fraction Collector

This chapter explains the operational parameters of the module.

6 Troubleshooting and Diagnostics

This chapter gives an overview about the troubleshooting and diagnostic

features and the different user interfaces.

7 Error Information

This chapter describes the meaning of error messages, and provides

information on probable causes and suggested actions how to recover from

error conditions.

4 Preparative Valve-Based Fraction Collector

In This Guide

8 Maintenance

This chapter gives you an overview and instructions about the possible

maintenance and repair procedures that can be performed by the user.

9 Parts and Materials

This chapter provides information on parts and materials.

10 Identifying Cables

This chapter provides information on cables used with the module.

11 Hardware Information

This chapter describes the module in more detail on hardware and electronics.

12 Appendix

This chapter provides additional information on safety, legal, and web.

Preparative Valve-Based Fraction Collector 5

Contents

1 Introduction to the Valve-Based Fraction Collector 9

Product Description 10

Features 11

Typical Applications 12

Mounting Examples 13

2 Site Requirements and Specifications 15

Site Requirements 16

Physical Specifications 19

Performance Specifications 20

3 Installing the Preparative Valve-based Fraction Collector 21

Unpacking the module 22

Installing the Preparative Valve-Based Fraction Collector 24

Flow connections with the Preparative Valve-Based Fraction Collector 31

4 Using the Preparative Valve-Based Fraction Collector 33

Configuration and Operation of the Fraction Collector 34

Solvent Information 36

Status Indicators 42

5 Preparing the Preparative Valve-Based Fraction Collector 43

Leak and Waste Concept 44

Best Practices 45

Setting up the Preparative Valve-Based Fraction Collector with the Instrument

Control Interface 46

Method Parameter Settings 52

Pooling 62

6 Preparative Valve-Based Fraction Collector

Contents

6 Troubleshooting and Diagnostics 63

User Interfaces 64

Agilent Lab Advisor Software 65

Hardware Symptoms 66

7 Error Information 69

What Are Error Messages 70

General Error Messages 71

Module Specific Error Messages 76

8 Maintenance 79

Introduction to Maintenance 80

Cautions and Warnings 81

Overview of Maintenance 83

Cleaning the Module 84

Replacing the Fuses of the Preparative Valve-Based Fraction Collector 85

Replacing the Module Firmware 87

9 Parts and Materials 89

Valve Drive Parts 90

Accessory Kit 91

10 Identifying Cables 93

Cable Overview 94

Analog Cables 96

Remote Cables 98

CAN/LAN Cables 102

RS-232 Cables 103

USB 104

Preparative Valve-Based Fraction Collector 7

Contents

11 Hardware Information 105

Electrical Connections 106

Interfaces 109

Configuration Switch Settings of the Preparative Valve-Based Fraction

Collector 116

12 Appendix 119

General Safety Information 120

Waste Electrical and Electronic Equipment Directive 127

Radio Interference 128

Sound Emission 129

Agilent Technologies on Internet 130

8 Preparative Valve-Based Fraction Collector

Contents

Preparative Valve-Based Fraction Collector

Agilent Technologies

Introduction to the Valve-Based

Fraction Collector

Product Description 10

Features 11

Typical Applications 12

Mounting Examples 13

This chapter gives an introduction to the module, instrument overview and

internal connectors.

10 Preparative Valve-Based Fraction Collector

Introduction to the Valve-Based Fraction Collector

Product Description

The Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector

(G7166A) comprises 11 collection valves, one waste valve and one solvent inlet

port. Solvent coming from a preparative HPLC system can be diverted into

11 different fractions depending on the software/firmware settings. The valves

can then be blown clean by allowing compressed air or nitrogen to be passed

through the same valves.

No fraction racks are required, as collection takes place into larger collection

bottles that can be placed anywhere within reach of the tubing outlets. The

Preparative Valve-Based Fraction Collector can be used in addition to a

fraction collector.

Figure 1 Overview of front

Valve

Clamps (attachable to both sides)

Leak sensor

Leak plane (attachable)

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