Zeiss LSM 510 Manual de usuario

LSM 510 / LSM 510

DuoScan

LSM 510 META / LSM 510 META

DuoScan

Release 4.0

March 2006

INTRODUCTION LSM 510 DuoScan

Carl Zeiss LSM 510 META DuoScan

II B 45-0021 e 03/06

Knowledge of this manual is required for the operation of the instrument. Would you therefore please

make yourself familiar with the contents of this manual and pay special attention to hints concerning the

safe operation of the instrument.

The specifications are subject to change; the manual is not covered by an update service.

communication of its contents are not permitted. Violations will entail an obligation to pay

compensation.

Developed in

Collaboration with European Molecular Biology Laboratory (EMBL)

PF 102209

Meyerhofstr. 1

69012 Heidelberg

GERMANY

Phone: ++49-6221-387-0

Telefax: ++49-6221-387-306

Issued by Carl Zeiss MicroImaging GmbH

07740 Jena, Germany

Phone: +49 (0) 3641 64 3400

Fax: +49 (0) 3641 64 3144

E-mail: [email protected]

www.zeiss.de/lsm

LSM 510 INTRODUCTION

LSM 510 META Carl Zeiss

03/06 B 45-0021 e III

How to make best use of the LSM 510 and LSM 510 META operating instructions:

This operating manual also includes the instructions for the LSM 510 META DuoScan and the

LSM 510 DuoScan systems.

A few symbols in these operating instructions will help you to recognize the nature and purpose of

information immediately:

The WARNING symbol warns against hazards for the user that might arise when operating the

laser.

This WARNING symbol warns against hazards from dangerously high voltages.

The CAUTION symbol warns against faults and hazards that might arise during operation and

which might cause damage to the unit.

The NOTE symbol will help you to optimally solve your work problem. It represents a practical

tip which will help you to find out which settings and methods are capable of improving or

accelerating a procedure.

The HOT SURFACE symbol warns against hazards for the user that might arise when touching

the lamp housing during operation.

The MAINS PLUG symbol remembers service personal to pull the mains plug before opening the

device housing.

Depending on the problem, these operating instructions will supply you with various possibilities:

• If you want to know where to find certain general areas of information, refer to the following outline

of sections to get a general overview.

• You will find a detailed table of contents at the start of every chapter. There you will see at a glance

what topics are dealt with in detail.

Always remember: The time you invest in getting acquainted with the product will pay

for itself many times over in your application task.

INTRODUCTION LSM 510 DuoScan

Carl Zeiss LSM 510 META DuoScan

IV B 45-0021 e 03/06

Contents

1 Notes on Device Safety

This section contains general notes on device safety, safe operation, and possible hazards

caused by failure to observe the instructions.

2 LSM 510 and LSM 510 META - Setup Requirements

The Setup Requirements section outlines the installation and supply requirements of the

LSM 510 and LSM 510 META Microscope Systems, together with the relevant specifications.

3 Introduction to Laser Scanning Microscopy

Here you will find an introduction to Laser Scanning Microscopy, with an explanation of the

principles of confocal imaging. The section also outlines the ways to present LSM image series

in three dimensions, and introduces you to the performance features of your LSM 510 or

LSM 510 META.

4 Operation in Expert Mode

In the Operation section you will find the most important steps and procedures of the

LSM menu structure. The step-by-step description how to get an image will be shown by

typical application examples including the WINDOWS XP graphic user environment.

5 VBA Programming for LSM

6 Routine Mode and Tools

This section contains a description of the Routine Mode for scanning images using the

LSM scanning module and the use of the tools for setting the microscope.

7 3D for LSM 510 and LSM 510 META

This section contains a description of the LSM 3D software package (basic program and add-

ons). At the same time, all functions and settings are presented in a systematic form and in the

order in which they can be reached from the basic menu via sub-menus and dialog boxes.

8 Annex

The annex contains the Application-specific Configurations, special notes and information for

using the LSM microscope and the brochure The confocal Laser Scanning Microscopy.

9 Multiphoton Laser Scanning Microscopy - Using the Zeiss LSM 510 META NLO

10 Certification

11 Brief Operating Manual

This section contains the brief instructions of the LSM 510 and LSM 510 META Microscope

Systems.

12 Laser safety warning labels

LSM 510 NOTES ON DEVICE SAFETY

LSM 510 META General Carl Zeiss

03/06 B 45-0021 e 1-1

CHAPTER 1 NOTES ON DEVICE SAFETY

CONTENTS

Page

1NOTES ON DEVICE SAFETY .............................................................................................1-2

1.1 General..............................................................................................................................1-2

1.2 Warning and Information Labels ........................................................................................1-3

1.3 Regulations ......................................................................................................................1-11

1.4 Notes on Setting up the Microscope System.....................................................................1-12

1.5 Power Requirements ........................................................................................................1-14

1.6 Notes on Handling the Laser Components........................................................................1-15

1.7 Physical Dimensions .........................................................................................................1-17

1.8 Environmental Requirements............................................................................................1-17

1.9 Notes on Handling the Computer and Data Media ...........................................................1-18

1.10 Notes on Care, Maintenance and Service .........................................................................1-19

1.11 User Interface...................................................................................................................1-20

1.11.1 Mounting and Dismounting Lamps, TPMT and Switching Mirror ......................................1-20

1.11.2 Mounting and Dismounting the Scan Head ......................................................................1-21

NOTES ON DEVICE SAFETY LSM 510 DuoScan

Carl Zeiss General LSM 510 META DuoScan

1-2 B 45-0021 e 03/06

1 NOTES ON DEVICE SAFETY

1.1 General

The LSM 510 and LSM 510 META laser scanning microscope, including its original accessories and

compatible accessories from other manufacturers, may only be used for the purpose of microscopic

techniques.

Laser Scanning Microscopes (LSM) are intended for high resolution imaging of biological or material

samples, whereby in contrast to wide field microscopy the specimen is illuminated raster-fashion with a

focused laser beam and the optical arrangement prevents light from out-of-focus regions of the

specimen contributing to image formation.

Installation and commissioning of the LSM 510 and LSM 510 META system must be performed

by authorized Carl Zeiss service staff. The system should not be used prior to instruction by a

Carl Zeiss representative.

The manufacturer will not assume liability for any malfunction or damage caused by anything

other than the intended use of the LSM 510 or LSM 510 META or individual modules or parts

of it, nor by any repair or other service operation performed or attempted by persons other

than duly authorized service staff. Any such action will invalidate any claim under warranty,

including parts not directly affected by such action. This also includes the modification of the

system computer with new cards, etc. by the user. The use of a camera at the base port of

Axiovert 200 M Combi stands is not allowed for reasons of laser safety. Any manipulation will

result in the loss of warranty of laser safety.

Please read also the notes on device safety and manuals of the microscope, the HBO, the HAL and

additional optional devices, if ordered, as the UV Laser, the piezo focusing device, the heating inserts and

the Ti:Sa Laser.

⇒ As the system is largely operated via menus on a computer, you should be familiar with the

principles of the operating system and its WINDOWS, WINDOWS 2000 or Windows XP graphical

user interface. The respective manuals are supplied together with the programs.

The LSM 510 and LSM 510 META are devices that belong to laser hazard class 3B. The systems

are equipped with safety interlocks that comply with laser hazard class 3B and 4. If equipped

with a Ti:Sa Laser (see list in section 1.6), the LSM 510 and LSM 510 META are devices that

belong to laser hazard class 4. WHO recommendations concerning health and industrial

protection when handling laser devices must be observed. The operator of the unit must also

observe all and any relevant statutory accident prevention regulations. The user is referred to

the safety data sheet provided together with the manual.

LSM 510 NOTES ON DEVICE SAFETY

LSM 510 META Warning and Information Labels Carl Zeiss

03/06 B 45-0021 e 1-3

1.2 Warning and Information Labels

The warning and information labels attached on the LSM 510 and LSM 510 META must be

observed. Check whether all of the labels shown below are provided on your instrument, and

contact Carl Zeiss Germany or one of the service agencies if you should discover that any of the

labels should be missing. You will receive a free replacement.

Description of labels

Caution: Faults and hazards that might arise during operation which might cause damage to

the unit or injury to the user.

Attention: Laser irradiation hazards possible when operating the system.

Attention: High voltage.

Pull the mains plug before opening the device housing.

Caution: Hot surface.

Caution: UV radiation.

Caution: Fingers can be caught.

The arrow points to the opening where laser light comes out during operation of the system.

Other labels on the system include one of the above depicted symbols and a detailed

description of the handling instructions. See also the following drawings of the system parts.

NOTES ON DEVICE SAFETY LSM 510 DuoScan

Carl Zeiss Warning and Information Labels LSM 510 META DuoScan

1-4 B 45-0021 e 03/06

1:1

ZERO

FOCUS

OBJECTIVE

REFLECTOR

Fig. 1-1 Warning and information labels on the Axiovert 200 M microscope with the LSM 510 META

scanning module

LSM 510 NOTES ON DEVICE SAFETY

LSM 510 META Warning and Information Labels Carl Zeiss

03/06 B 45-0021 e 1-5

Fig. 1-2 Warning and information labels on the Axio Imager.Z1 microscope with LSM 510 META

scanning module

NOTES ON DEVICE SAFETY LSM 510 DuoScan

Carl Zeiss Warning and Information Labels LSM 510 META DuoScan

1-6 B 45-0021 e 03/06

100

Fig. 1-3 Warning and information labels on the Axioskop 2 FS MOT microscope with the

LSM 510 META scanning module

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