Waters Prep 150 LC System Manual de usuario

Prep 150 LC System

Quick Start

715003940 / Revision B

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ii August 8, 2014, 715003940 Rev. B

General Information

OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT

THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and should

not be construed as a commitment by Waters Corporation. Waters Corporation

assumes no responsibility for any errors that may appear in this document. This

document is believed to be complete and accurate at the time of publication. In no

event shall Waters Corporation be liable for incidental or consequential damages

in connection with, or arising from, its use. For the most recent revision of this

document, consult the Waters Web site (waters.com).

Trademarks

Waters and “THE SCIENCE OF WHAT’S POSSIBLE.” are registered trademarks

of Waters Corporation, and ChromScope is a trademark of Waters Corporation.

Other registered trademarks or trademarks are the sole property of their owners.

Customer comments

Waters’ Technical Communications organization invites you to report any errors

that you encounter in this document or to suggest ideas for otherwise improving

it. Help us better understand what you expect from our documentation so that we

can continuously improve its accuracy and usability.

We seriously consider every customer comment we receive. You can reach us at

tech_comm@waters.com.

Contacting Waters

Contact Waters® with enhancement requests or technical questions regarding the

use, transportation, removal, or disposal of any Waters product. You can reach us

via the Internet, telephone, or conventional mail.

August 8, 2014, 715003940 Rev. B iii

Waters contact information:

Internet The Waters Web site includes contact information for Waters loca-

tions worldwide.

Visit www.waters.com

Telephone

and fax

From the USA or Canada, phone 800 252-HPLC, or fax 508 872

1990.

For other locations worldwide, phone and fax numbers appear in

the Waters Web site.

Conventional

mail

Waters Corporation

34 Maple Street

Milford, MA 01757

USA

Safety considerations

Some reagents and samples used with Waters instruments and devices can pose

chemical, biological, or radiological hazards (or any combination thereof). You

must know the potentially hazardous effects of all substances you work with.

Always follow Good Laboratory Practice, and consult your organization’s safety

representative for guidance.

FCC radiation emissions notice

Changes or modifications not expressly approved by the party responsible for

compliance, could void the users authority to operate the equipment. This device

complies with Part 15 of the FCC Rules. Operation is subject to the following two

conditions: (1) this device may not cause harmful interference, and (2) this device

must accept any interference received, including interference that may cause

undesired operation.

Canada spectrum management emissions notice

This class A digital product apparatus complies with Canadian ICES-003.

Cet appareil numérique de la classe A est conforme à la norme NMB-003.

Electrical power safety notice

Do not position the instrument so that it is difficult to operate the disconnecting

device.

iv August 8, 2014, 715003940 Rev. B

Safety hazard symbol notice

Documentation needs to be consulted in all cases where the symbol is used to

find out the nature of the potential hazard and any actions which have to be

taken.

Equipment misuse notice

If the equipment is used in a manner not specified by the manufacturer, the protec-

tion provided by the equipment may be impaired.

Safety advisories

Consult Appendix A for a comprehensive list of warning and caution advisories.

Operating the Prep 150 LC system

When operating the Prep 150 LC system, follow standard quality-control (QC)

procedures and the guidelines presented in this section.

Applicable symbols

Symbol Definition

Manufacturer

Date of manufacture

Authorized representative of the European Community

Confirms that a manufactured product complies with all applic-

able European Community directives

Australia EMC compliant

Confirms that a manufactured product complies with all applic-

able United States and Canadian safety requirements

Consult instructions for use

August 8, 2014, 715003940 Rev. B v

Symbol Definition

Supply ratings

Electrical and electronic equipment with this symbol may

contain hazardous substances and should not be disposed of as

general waste.

For compliance with the Waste Electrical and Electronic Equip-

ment Directive (WEEE) 2012/19/EU, contact Waters Corpor-

ation for the correct disposal and recycling instructions.

Safety information

Consult the operator's guides of the instruments or devices associated with this

system for information about how to safely operate and maintain them.

ISM classification

ISM Classification: ISM Group 1 Class B

This classification has been assigned in accordance with IEC CISPR 11 Industrial

Scientific and Medical (ISM) instruments requirements. Group 1 products apply to

intentionally generated and/or used conductively coupled radio-frequency energy

that is necessary for the internal functioning of the equipment. Class B products

are suitable for use in both commercial and residential locations and can be

directly connected to a low voltage, power-supply network.

EC authorized representative



 

Waters Corporation

Stamford Avenue

Altrincham Road

Wilmslow SK9 4AX UK



 

Telephone:

Fax:

Contact

+44-161-946-2400

+44-161-946-2480

Quality manager

vi August 8, 2014, 715003940 Rev. B

Preface

This guide is part of the documentation set for the Waters Prep 150 LC system. It

provides you with basic information about the major tasks you can perform with

the Prep 150 LC system. For detailed information, refer to the Prep 150 LC

system's Online Information System that you can access by clicking Help in the

ChromScope software.

Audience and purpose

Waters designed the Prep 150 LC system for "walk-up" use by chemists who must

meet purification requirements. The system is particularly effective where rapid

preparative separations of complex mixtures are required to establish initial work-

flow criteria. It is not intended for use in diagnostic or biologically hazardous

applications.

Use the system to perform various sampling tasks in these environments:

lPharmaceutical development

lQuality assurance and quality control

lChemical materials

lEnvironmental sciences

lAcademic laboratories

Documentation conventions

Instructions in this documentation adhere to the following conventions.

>Denotes the path for selecting menu commands, for moving from one

menu to another, or for performing a task.

Example: Click File > Open

< > Indicates that you must enter variable information, such as a path or

filename.

Example: Type open <filename>

August 8, 2014, 715003940 Rev. B vii

viii August 8, 2014, 715003940 Rev. B

Preface

Prep 150 LC System

This quick-start guide presents nine elementary procedures that provide a basic

knowledge of the Prep 150 LC system's operation and capabilities. Though relat-

ively broad in scope, the guide offers enough instruction to allow purification of

product compounds and collection of fractions. With the information in this guide,

you can experiment with the system, learning for yourself minor details and vari-

ations on the prescribed practices. For more detailed information about each of the

procedures, open the online Help window in the ChromScope software, and browse

the list of topics there.

The nine procedures appear as individual chapters:

1Start the system

2 Set up your work environment

3 Verify the system and module settings

4 Select and run a method-screening sample list

5 Create and load the run's methods and sample list

6 Start and monitor the run

7 Analyze the run's data

8 Report the results

9 Prepare the system for the next run or for shutting down

August 8, 2014, 715003940 Rev. B 9

Start the system

To start the Prep 150 LC system and prepare it for operation, follow this proced-

ure in conjunction with Good Laboratory Practice (GLP) guidelines and your labor-

atory's standard operating procedures.

Starting sequence:

To start the system and prepare it for operation:

1. Power-on the individual system modules by actuating the power switch on

each system module.

Important: If you are using the optional Prep Degasser with the 2545 QGM,

ensure the degasser is operating.

2. Power-on the ChromScope PC workstation.

Requirement: You must wait at least 30 seconds after powering-on the last

module before you start the ChromScope software and Waters Instrument

Console. Doing so is necessary for the components to communicate properly

with the software during startup.

3. From the Windows desktop, double-click the ChromScope shortcut .

Alternative: From the Windows Start menu, click All Programs, and click

ChromScope.

10 August 8, 2014, 715003940 Rev. B

Prep 150 LC System

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