Waters Extraction+ Manual de usuario
USER MANUAL
©2022 Waters Corporation
Extraction+
Safety instructions
• Do not use this device for:
- evacuating biological habitats
- evacuating explosive, corrosive, or similar gases
• The device is not suitable for:
- inating/deating objects
- pumping liquids
- use in potentially explosive atmospheres
- underwater usage
- underground usage
• Set up the Extraction+ vacuum pump in a spacious area on an even,
stable, clean, nonslip, dry surface.
• Completely switch o the device and remove cables before
moving it.
• The user must maintain a distance of at least 20 cm from the
product (WiFi antenna) during installation or operation.
• The device is intended for indoor use only.
• With the exception of lter cartridge replacement, device
maintenance is exclusively reserved for Waters servicing and support
representatives.
• If the device is used in any way other than those specied by the
manufacturer, the user protection provided by the device may be
impaired.
• The safety of the user cannot be ensured if modications are made
to the device or parts of the device by third parties.
• All input/output ports are Safety Extra Low Voltage (SELV) circuits.
You should only connect SELV circuits to other SELV circuits.
• If the device overheats, it will stop operating. If this occurs,
immediately disconnect the device from its power supply.
About this User Manual
Read the User Manual before using this device. Strictly follow usage and maintenance
instructions provided. It is the user’s responsibility to become familiar with all available
information concerning the correct use, care, and limitations of these products. If you are
uncertain about the correct use or limitations of the products, contact Waters service and
support. The manufacturer, the distributor, and their respective owners, employees, agents, and
representatives are not responsible or liable for errors or omissions.
Information in this User Manual is subject to change without notice and does not represent a
commitment on the part of Waters. Waters assumes no responsibility for any inaccuracies that
may be contained in this User Manual. Waters makes no commitment to update or keep current
the information in this User Manual, and reserves the right to make improvements to this User
Manual or to the products described herein at any time without notice.
If you nd information in this manual that is incorrect, misleading, or incomplete, we would
appreciate your comments and suggestions.
Product overview & setup
Extraction+ vacuum pump
Extraction+ manifold
Built-in wireless
adapter
Built-in
computer
LED indicator Vacuum inlet Power button
Rear view
Ethernet for
cable
connection to
network
Maintenance hatch
Waste container
(can substitute any
GL45-threaded
bottle)
Silicone
vacuum
hose
Collar lifter
Extraction labware
(column in adapter or
cartridge)
Collection labware
(tubes in adapter
or plate with manifold spacers)
Manifold base, waste
collector
Manifold collar
Power socket
Getting started
A. Install the vacuum pump and liquid trap system
Connect the Extraction+ vacuum pump
and the waste container using the silicone hose
If needed, cut the silicone hoses to the appropriate lengths
3
4
Bottle smaller than one
liter can also be placed
on the Extraction+
vacuum pump.
On or under the workbench,
or on the oor.
On or under the workbench,
or on the oor.
We recommend using the longest portion of
the hose for connecting the Extraction+ vacuum
pump to the waste container.
Place the Extraction+ vacuum
pump
Place the waste container
(GL45-threaded bottle)
1 2
Ensure that the connection is loose
enough to prevent the waste container
from falling.
This hose must be connected to the
shorter port of the thread.
B. Installation of the manifold
Setup for cartridge
When used with the Andrew+ Pipetting Robot,
the Extraction+ vacuum manifold is always
placed at the same deck location:
Domino location 5
Setup for lter plate
Plug the silicone hose between
the waste container and the
Extraction+ manifold
Plug the silicone hose between
the waste container and the
Extraction+ manifold
Place the manifold spacers into the waste
collector (see additional instructions on
the next page)
Place the manifold collar lifter
Place the manifold collar lifter
Place the manifold collar
Place the manifold collar
Place the cartridge adapter as OneLab
prompts you to
Place the lter plate as OneLab prompts
you to
1
1
3
2
2
4
4
3
5
This hose must be connected to
the elbow port of the thread.
This hose must be connected to
the elbow port of the thread.
B.1. Manifold spacers (only for use with lter plate)
The plate spacers allow ne-tuning of the
penetration height of the lter plate tips within the
wells of the collection labware. Ideal penetration
is 2-3 mm.
The spacer system is composed of three sizes:
The spacers can be
placed into the manifold
waste collector...
...and can be combined to
further prop up collection
plates if necessary
(example here:
3 mm spacer
+ 15 mm spacer)
Examples of optimal spacer usage
for dierent lter and collection consumable combinations
3 mm 15 mm 17 mm
Extraction plate
skirt length
Collection
consumable
Size 1:
Small skirt
Size 2:
Long skirt
Waters 2-mL 96-square well
collection plate, cut corner H1
1 × 3 mm spacer 1 × 17 mm spacer
on top of 1 × 3 mm spacer
Waters 0.7-mL 96-round well
collection plate
1 × 15 mm spacer 1 × 17 mm spacer
on top of 1 × 15 mm spacer
Waters QuanRecovery™ 700-µL
96-well plate
1 × 15 mm spacer 1 × 17 mm spacer
on top of 1 × 15 mm spacer
Waters 0.8-mL 96-round well
collection plate
1 × 15 mm spacer 1 × 17 mm spacer
on top of 1 × 15 mm spacer
Extraction labware tip
Collection labware
Tip penetration
2–3 mm
Small skirt
Long skirt
B.2. Use of plate sealer (only for lter plate use)
To apply vacuum evenly on each lter plate
cavity, we recommend placing a plate-sealer
lm on top of unused lter plate cavities.
To apply vacuum evenly on each cartridge,
we recommend placing sealing caps in
each of the unused slots of the cartridge
adapter.
When using cartridges as
lter consumables during
an automated run with the
Andrew+ robot, OneLab
prompts the usage of a
Collection Labware Rack Domino
in addition to the collection
rack itself.
Ordering info: Adhesive Seal for 96-well
and 384-well Plates, 100/pk, Waters p/n:
186006336C
Contact your Waters representative.
Ordering info: Microplate Gripper (Tool),
Waters p/n: 186009776
Contact your Waters representative.
This Domino holds the collection consumables
during the rst phases of the solid-phase
extraction, such as: conditioning, loading, and
washing.
The Collection Labware Rack Domino is included
in each Extraction+ Cartridge Kit. You can also
purchase it separately.
Sample available in the packaging
B.4. Collection labware rack (only for cartridge use)
B.3. Use of sealing caps (only for cartridge use)
B.5. Labware gripper (only for Andrew+ use)
Cut o the desired dimensions and
apply the lm on the plate over the
unused cavities
Add the Microplate Gripper
tool on the Andrew+
robot tool rack to ensure
automated movement of
collection labware and racks
into and out of the manifold.
C. Connection to OneLab
If you have a OneLab account, plug
your Extraction+ vacuum pump
into a power source.
Wait for the LED indicator to
turn blue and blink.
After a couple of minutes,
the Extraction+ vacuum
pump will inform you
when it is in installation
mode.
Connect your computer, tablet, or smartphone to the Extraction+ vacuum pump.
Launch a Web browser, type http://192.168.2.1,and follow the on-
screen steps to continue installation.
A. Go to your WiFi settings
and select the network named Extraction-xxx.xxxx.xxxxx.
1 2
3
4
scan me
Note that the installa-
tion page might open
automatically.
If you do not have access to a
WiFi-compatible device, refer to
our online Help:
help.andrewalliance.com
!
!
xxxx.xxxxx is the Extraction+ device serial number (found on the back of the
Extraction+ vacuum pump or on top of the Extraction+ vacuum manifold).
B. The LED indicator stops blinking when the device is successfully
connected.
Extraction-xxx.xxxx.xxxxx
If the Extraction+ vacuum pump does not
automatically enter installation mode,
press the power button ve times.
!
http://192.168.2.1
Before
you begin
If you do not have a OneLab account, create one at:
https://onelab.com/signup
The device will automatically start up.
Only use the provided AC/DC
power supply unit and cords.
Operations
Design your protocol
Execute experiment with
Extraction+
Start the experiment and let OneLab
control your device
OneLab automatically selects your
Extraction+ and allows you to use it with each
available experiment setup, manual, semi-
automated, or fully automated operation.
Choose your desired experiment setup, and
then prepare all the required materials.
Our drag-n-drop editor makes it easy to
design a precise and complete protocol with all
you need to run your
experiment perfectly.
Create a Flow-through extraction
action by clicking this menu, which
is located on the left side of the
screen.
Actions
Follow the visual instructions based on the
protocol you are using and you will be guided
through your experiment.
Each step of the experiment is recorded and
available in OneLab.
Feel free to contact
the Waters
support team Send us an email at
Chat with our support team
directly in OneLab
We reply within one business day
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