Waters Pump Control Module II Manual de usuario

Waters Pump Control

Module II

Installation Guide

71500078604/Revision B

OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM

WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and

should not be construed as a commitment by Waters Corporation. Waters

Corporation assumes no responsibility for any errors that may appear in this

document. This document is believed to be complete and accurate at the time

of publication. In no event shall Waters Corporation be liable for incidental or

consequential damages in connection with, or arising from, its use.

Trademarks

Millennium and Waters are registered trademarks of Waters Corporation,

and Empower, LAC/E, SAT/IN, and “THE SCIENCE OF WHAT’S

POSSIBLE.” are trademarks of Waters Corporation.

Other registered trademarks or trademarks are the sole property of their

owners.

iii

Customer comments

Waters’ Technical Communications department invites you to tell us of any

errors you encounter in this document or to suggest ideas for otherwise

improving it. Please help us better understand what you expect from our

documentation so that we can continuously improve its accuracy and

usability.

We seriously consider every customer comment we receive. You can reach us

at tech_comm@waters.com.

Contacting Waters

Contact Waters®with enhancement requests or technical questions regarding

the use, transportation, removal, or disposal of any Waters product. You can

reach us via the Internet, telephone, or conventional mail.

Safety considerations

Some reagents and samples used with Waters instruments and devices can

pose chemical, biological, and radiological hazards. You must know the

potentially hazardous effects of all substances you work with. Always follow

Good Laboratory Practice, and consult your organization’s safety

representative for guidance.

When you develop methods, follow the “Protocol for the Adoption of Analytical

Methods in the Clinical Chemistry Laboratory,” American Journal of Medical

Technology, 44, 1, pages 30–37 (1978). This protocol addresses good operating

procedures and the techniques necessary to validate system and method

performance.

Waters contact information

Contacting medium Information

Internet The Waters Web site includes contact

information for Waters locations worldwide.

Visit www.waters.com, and click Waters

Division > Contact Waters Online.

Telephone and fax From the USA or Canada, phone 800

252-HPLC, or fax 508 872 1990.

For other locations worldwide, phone and fax

numbers appear in the Waters Web site.

Conventional mail Waters Corporation

34 Maple Street

Milford, MA 01757

USA

Safety advisories

Consult Appendix A for a comprehensive list of warning and caution

advisories.

Operating the Waters Pump Control Module II

When operating the Pump Control Module II, follow standard quality-control

(QC) procedures and the guidelines presented in this section.

Applicable symbols

Audience and purpose

This guide is intended for use by individuals whose familiarity with HPLC

ranges from novice to expert. It describes how to install the Pump Control

Module II in a multi-pump gradient system with up to three pumps controlled

by a Waters data control system.

Intended use of the Pump Control Module II

Waters designed the Pump Control Module II to provide programmable

control through a Waters data system of up to three, 500-series high-pressure

pumps.

Calibrating

To calibrate LC systems, follow acceptable calibration methods using at least

five standards to generate a standard curve. The concentration range for

standards should include the entire range of QC samples, typical specimens,

and atypical specimens.

Symbol Definition

Confirms that a manufactured product complies

with all applicable European Community

directives

Australia C-Tick EMC Compliant

ABN 49 065 444 751

To calibrate mass spectrometers, consult the calibration section for the

operator’s guide of the instrument you are calibrating.

Quality-control

Routinely run three QC samples that represent subnormal, normal, and

above-normal levels of a compound. Ensure that QC sample results fall within

an acceptable range, and evaluate precision from day to day and run to run.

Data collected when QC samples are out of range might not be valid. Do not

report these data until you are certain that the instrument performs

satisfactorily.

ISM classification

ISM Classification: ISM Group 1 Class B

This classification has been assigned in accordance with CISPR 11 Industrial

Scientific and Medical, (ISM) instruments requirements. Group 1 products

apply to intentionally generated and/or used conductively coupled

radio-frequency energy that is necessary for the internal functioning of the

equipment. Class B products are suitable for use in both commercial and

residential locations and can be directly connected to a low voltage,

power-supply network.

Table of Contents 1

Trademarks ............................................................................................................ ii

Customer comments ............................................................................................ iii

Contacting Waters ............................................................................................... iv

Safety considerations .......................................................................................... iv

Safety advisories.................................................................................................. v

Operating the Waters Pump Control Module II ......................................... i-v

Applicable symbols .............................................................................................. v

Audience and purpose.......................................................................................... v

Intended use of the Pump Control Module II..................................................... v

Calibrating ........................................................................................................... v

Quality-control.................................................................................................... vi

ISM classification .............................................................................................. i-vi

ISM Classification: ISM Group 1 Class B ......................................................... vi

1 Overview .................................................................................................. 1-1

Introduction ....................................................................................................... 1-2

System configurations ..................................................................................... 1-3

Ethernet configuration.................................................................................... 1-3

IEEE-488 configuration................................................................................... 1-4

Signal conditions .............................................................................................. 1-5

2 Installing the Pump Control Module II ............................................ 2-1

Selecting the site and unpacking .................................................................. 2-2

Selecting the site.............................................................................................. 2-2

Unpacking and inspecting the module ........................................................... 2-2

Rear-panel signal connections ....................................................................... 2-3

Ethernet signal cable connections .................................................................. 2-4

Table of Contents

2 Table of Contents

IEEE-488 signal cable connections................................................................. 2-5

Connecting the pumps..................................................................................... 2-7

Connecting the event cables............................................................................ 2-8

Powering-on the module ............................................................................... 2-12

Programming the module ............................................................................. 2-14

Maintenance and troubleshooting .............................................................. 2-14

Cleaning the cabinet...................................................................................... 2-14

Replacing the fuse.......................................................................................... 2-14

Troubleshooting ............................................................................................. 2-15

A Safety Advisories .................................................................................. A-1

Warning symbols ............................................................................................... A-2

Task-specific hazard warnings........................................................................ A-2

Warnings that apply to particular instruments, instrument components, and

sample types............................................................................................... A-3

Caution symbol .................................................................................................. A-5

Warnings that apply to all Waters instruments ......................................... A-5

Electrical and handling symbols ................................................................. A-12

Electrical symbols.......................................................................................... A-12

Handling symbols .......................................................................................... A-13

B Specifications ........................................................................................ B-1

Physical and environmental specifications ............................................... B-2

Accuracy and precision .................................................................................. B-2

Electrical specifications ................................................................................. B-3

Protection Class 1 ............................................................................................ B-3

Overvoltage Category II .................................................................................. B-3

Pollution Degree 2 ........................................................................................... B-3

Moisture protection – Normal (IPXO) ............................................................ B-3

AC-to-DC power specifications ..................................................................... B-4

C Spare Parts ............................................................................................ C-1

1-1

1Overview

Contents

Topic Page

Introduction 1-2

System configurations 1-3

Signal conditions 1-5

1-2 Overview

Introduction

The Waters®Pump Control Module II is an HPLC pump controller. Through

the medium of Waters data system software, it offers programmatic control of

high-pressure-mixing pumps in HPLC and MS systems.

Pump Control Module II

Depending on your pump configuration requirements, you can attach as many

as three Waters 510 or 515 HPLC Pumps to the module and regulate the

solvent composition and flow rates by programming instrument method

settings in Empower, Millennium, or MassLynx data control software. (For

the module’s firmware/software requirements, see the Waters Pump Control

Module II release notes.)

A single Waters 510 or 515 Pump in an HPLC system is ideal for

high-throughput isocratic analyses. When you control two or three of these

pumps through a data system and external controller, you can achieve

reproducible, multipump gradient delivery with exceptionally smooth

concurrent-stream blending.

TP02342

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