Star Lab ErgoOne Manual de usuario

ErgoOne – Adjustable Volume Pipette

English ..................................................... 1

Deutsch ...................................................23

Français ..................................................45

Italiano ...................................................67

Español ...................................................89

This is your

ErgoOne®

User Manual.

STARLAB International GmbH

Neuer Höltigbaum 38

22143 Hamburg

Germany

T: +49 (0)40 675 99 39 0

F: +49 (0)40 675 99 39 20

[email protected]

www.starlabgroup.com

STARLAB (UK), Ltd

5 Tanners Drive

Milton Keynes MK14 5BU

United Kingdom

T: +44 (0)1908 283800

F: +44 (0)1908 283802

[email protected]

www.starlab.co.uk

STARLAB GmbH

Neuer Höltigbaum 38

22143 Hamburg

Germany

T: +49 (0)40 675 99 39 0

F: +49 (0)40 675 99 39 20

[email protected]

www.starlab.de

STARLAB FRANCE SARL

30 Rue Jean Rostand

91400 Orsay

France

T: +33 (0)1 60 13 71 70

F: +33 (0)1 69 41 48 65

[email protected]

www.starlab.fr

STARLAB S.r.l.

Via Pinturicchio 1

20133 Milano

Italy

T: +39 (0)2 7020 1040

F: +39 (0)2 7020 1033

[email protected]

www.starlab.it

Passionate for science.

IMEO-INT-INT-07-2020

ErgoOne – Adjustable Volume Pipette

English ..................................................... 3

Deutsch ................................................... 25

Français .................................................. 47

Italiano ................................................... 69

Español ................................................... 91

This is your

ErgoOne®

User Manual.

STARLAB International GmbH

Neuer Höltigbaum 38

22143 Hamburg

Germany

T: +49 (0)40 675 99 39 0

F: +49 (0)40 675 99 39 20

[email protected]

www.starlabgroup.com

STARLAB (UK), Ltd

5 Tanners Drive

Milton Keynes MK14 5BU

United Kingdom

T: +44 (0)1908 283800

F: +44 (0)1908 283802

[email protected]

www.starlab.co.uk

STARLAB GmbH

Neuer Höltigbaum 38

22143 Hamburg

Germany

T: +49 (0)40 675 99 39 0

F: +49 (0)40 675 99 39 20

[email protected]

www.starlab.de

STARLAB FRANCE SARL

30 Rue Jean Rostand

91400 Orsay

France

T: +33 (0)1 60 13 71 70

F: +33 (0)1 69 41 48 65

[email protected]

www.starlab.fr

STARLAB S.r.l.

Via Pinturicchio 1

20133 Milano

Italy

T: +39 (0)2 7020 1040

F: +39 (0)2 7020 1033

[email protected]

www.starlab.it

Passionate for science.

IMEO-INT-INT-07-2020

ErgoOne®User Manual 3

English

Table of Contents

Contents

ErgoOne®User Manual 3

1. Introduction ................................................... 4

2. Operation...................................................... 5

2.1 Description ..................................................... 5

2.2 Setting the Volume ............................................ 6

2.3 Pipetting.......................................................7-8

2.4 Pipette Identification.......................................... 9

2.5 Safety Notes ................................................... 9

3. Troubleshooting .............................................10

4. Cleaning and Decontamination..............................11

5. Technical Data .. ......... ... ..... ........ . .. ...... ........ 12

6. Calibration................................................13-14

7. Maintenance................................................. 15

7.1 Maintaining Single-Channel Pipettes ....................... 15

7.2 Maintaining Multi-Channel Pipettes ........................ 18

8. Ordering Information ....................................... 20

9. Pipette Tip Compatibility ................................... 22

10. Guarantee ................................................... 24

11. Intended use ................................................ 24

ErgoOne – Adjustable Volume Pipette

English ..................................................... 1

Deutsch ...................................................23

Français ..................................................45

Italiano ...................................................67

Español ...................................................89

This is your

ErgoOne®

User Manual.

STARLAB International GmbH

Neuer Höltigbaum 38

22143 Hamburg

Germany

T: +49 (0)40 675 99 39 0

F: +49 (0)40 675 99 39 20

[email protected]

www.starlabgroup.com

STARLAB (UK), Ltd

5 Tanners Drive

Milton Keynes MK14 5BU

United Kingdom

T: +44 (0)1908 283800

F: +44 (0)1908 283802

[email protected]

www.starlab.co.uk

STARLAB GmbH

Neuer Höltigbaum 38

22143 Hamburg

Germany

T: +49 (0)40 675 99 39 0

F: +49 (0)40 675 99 39 20

[email protected]

www.starlab.de

STARLAB FRANCE SARL

30 Rue Jean Rostand

91400 Orsay

France

T: +33 (0)1 60 13 71 70

F: +33 (0)1 69 41 48 65

[email protected]

www.starlab.fr

STARLAB S.r.l.

Via Pinturicchio 1

20133 Milano

Italy

T: +39 (0)2 7020 1040

F: +39 (0)2 7020 1033

[email protected]

www.starlab.it

Passionate for science.

IMEO-INT-INT-07-2020

ErgoOne®User Manual4

1. Introduction

ErgoOne® variable volume pipettes are designed in accordance with

the latest ergonomic principles. The pipette is extremely easy to use

and has minimal operating forces to help prevent hand fatigue.

ErgoOne® provides accurate and precise pipetting in a variety of

volume ranges and is available in single-channel, eight-channel and

twelve-channel models.

At a glance:

•Good handling with ergonomic design

•Easy to use volume adjustment

mechanism

•Volume lock

•Minimal operating forces

•Extremely accurate and precise

•Lightweight

•Low-maintenance high-quality product

•Fully autoclavable

Introduction / Operation English

ErgoOne®User Manual

TipOne®

2. Operation

2.1 Description

Along with your pipette, you should

also have:

•Declaration of conformity with test

record

•Stickers for personalisation/

identication

•Auxiliary tool

(single-channels up to 1ml only)

•Pipette lters (5ml only)

Colour-coded volume control button with

volume adjustment mechanism

Tip ejector button

Alignment unit

Volume display

Ejector

xing

Tip ejector

Tip holder

To obtain the best results,

we recommend using original

TipOne® pipette tips.

2.2 Setting the Volume

Open the volume adjustment catch by

pulling up the volume control button 1

(Fig. 1), until you hear a click. Turn the

volume control button 1 clockwise to

reduce the aspirated volume, or counter

clockwise to increase it. The set volume is

shown in the four-step volume display 4

from top to bottom, with a white line indi-

cating the decimal point. When increasing

the volume setting, pass the required

value by 1/3 of a turn and then slowly de-

crease to reach the volume, making sure

not to go beyond the required volume.

After you have nished setting the volume,

push the volume control button 1 back to

its lock position until you hear a click. The

pipette is now ready to use.

Volume range ErgoOne®

ErgoOne® model Volume range (min./ max.) Colour code

2.5µl 0.1–2.5µl Red

10µl 0.5–10µl Red

20µl (micro cone) 2–20µl Red

20µl (standard cone) 2–20µl Yellow

100µl 10–100µl Yellow

200µl 20–200µl Yellow

300µl 30–300µl Green

1000µl 100–1000µl Blue

5000µl 500–5000µl Violet

Table 1

10.00µl 0.50µl 20.0µl 100µl200.0µl 1000µl

10µl Pipette 200µl Pipette 1000µl Pipette

Please note the minimum

and maximum volume

range of your pipette

(Table 1). You have

reached the end point of

the volume adjustment

when it becomes more

difcult to turn the button

and you can hear

a clicking sound.

Do not use excessive

force!

Fig.1

ErgoOne®User Manual6

Examples of typical volume displays:

ErgoOne®User Manual 7

English

Operation

2.3 Pipetting

Use the pipette only with tip attached!

Liquid aspiration

Set the required volume (see 2.2 Setting the volume) and attach the

appropriate size pipette tip(s) to the tip holder(s) 7:

•Press the volume control button 1 until the rst stop position.

•Hold the pipette upright and immerse approx. 3mm of the pipette

tip into the liquid.

•Allow the volume control button 1 to return slowly back to the

starting position.

•Hold the pipette tip in the liquid for a further 2 seconds to avoid

sucking in air.

•Remove the pipette tip from the liquid.

Dispensing fluids

•Place the pipette tip against the inner wall of the tube or vessel, at

an angle of 30 – 45°.

•Press the volume control button 1 slowly and evenly until it

reaches the rst stop position.

•Wait one second and press the volume control button 1 down to

the second stop position to empty the pipette tip fully (blow out).

•Keeping the volume control button 1 pressed down, lightly drag

the pipette tip up the inner wall of the tube to remove any residual

liquid.

•Allow the volume control button 1 to return slowly back to the

starting position.

•Press the tip ejector button 2 to eject the pipette tip.

ErgoOne®User Manual 7

ErgoOne®User Manual8

When pipetting critical viscous media, the precision and accuracy

can be increased through ‘reverse pipetting’. In reverse pipetting, a

larger volume is aspirated than that indicated on the volume display.

As a result, a small amount of residual liquid remains in the pipette

tip after the pipetting process. How to reverse pipette:

•Secure the pipette tip.

•Press the volume control button 1 until it reaches the second stop

position.

•Hold the pipette upright and immerse the pipette tip approx. 3mm

into the liquid.

•Allow the volume control button 1 to return slowly back to the

starting position.

•Hold the pipette tip in the liquid for a further 2 seconds to avoid

sucking in air.

•Remove the pipette tip from the liquid.

•Place the pipette tip against the inner wall of the tube or vessel,

at an angle of 30 – 45°.

•Press the volume control button 1 slowly and evenly until it

reaches the rst stop position (do not use the blow-out function).

•Keeping the volume control button 1

pressed, remove the pipette tip from the tube or vessel before

blowing out the residual liquid into a separate container.

•Allow the volume control button 1 to return slowly back to the

starting position.

•Press the tip ejector button 2 to eject the pipette tip.

Note: Pre-wetting

Some liquids (eg.solutions containing protein, organic solvents,

or viscous liquids) leave a lm on the inside of the pipette tip. To

ensure maximum precision and accuracy, STARLAB recommends

pre-wetting each new tip rst by aspirating and dispensing the

liquid two or three times. Alternatively, TipOne® RPT pipette tips are

special, surface-treated tips that ensure improved flow behaviour

for ‘tricky’ liquids.

Ask your STARLAB representative for more information.

Reverse pipetting

ErgoOne®User Manual 9

English

Operation

Your pipette is supplied with autoclave-resistant stickers in four

diffe rent colours. These can be attached to the surface above the

nger rest to personalise/help identify your pipette (Fig. 2).

Each ErgoOne® pipette has its

own serial number. This serial

number can be found behind the

tip ejector button 2 when it is

pressed down.

•Use the pipette only with tip(s) attached.

•Make sure that no liquid enters the pipette.

•Do not lay the pipette down when a pipette tip is xed and

contains liquid.

•Do not set the volume outside the specied range.

•Do not use any aggressive solvents or acetone to clean the pipette.

•Do not use cleaning agents in combination with autoclaving or

UV-Treatment.

•Only use original parts and accessories.

•Before pipetting aggressive chemicals or organic solvents, check

their compatibility with the pipette and pipette tips.

•Avoid temperature differences between the pipette and pipette tip

as this could lead to incorrect volume dispensing.

•Check the dispensing volume of liquids that strongly differ from

the physical properties of water, eg. with different density (see

also section 6 Calibration).

Fig.2

2.4 Pipetting

Identication

2.5 Safety Notes

The following table provides information about the possible causes

of problems with suggestions as to how these can be xed. If you are

not able to x the problem, your local STARLAB ofce will be happy

to help.

3. Troubleshooting

ErgoOne®User Manual10

Troubleshooting

Problem Possible cause Action

Pipette tip drips

Wrong pipette tip Use TipOne®

Tip is loose Attach the tip securely

Tip holder loose Tighten the tip holder

Debris between the tip

and tip holder

Clean the tip holder

and use new tip

Seals/O ring damaged Replace the affected seals/O rings

Debris between the

piston and seal

Clean the seal and piston and

re-lubricate

Piston insufciently lubricated Clean and re-lubricate the piston

Piston contaminated Clean and re-lubricate the piston

Piston damaged Replace piston and seal

Volume control button

sticks/not smooth

Piston contaminated Clean and re-lubricate the piston

Seals contaminated Unscrew lower housing, clean all seals

and replace if necessary

Leaked solvent vapours Unscrew the lower housing and allow to

air. Clean and lightly

lubricate the piston

Pipette blocked, not

enough intake of liquid

Liquid has penetrated

the tip holder and dried

Unscrew lower housing and rinse with

warm water. Then rinse with dist. water

and allow to dry

Replace tip holder

Drop formation on inner

wall of pipette tip

Uneven wetting of pipette tip Attach new pipette tip

Using pipette tips with poor

wetting properties

Use original STARLAB

TipOne®pipette tips

Pipetting volume incorrect

Tip taken from liquid too quickly Extract tip from liquid slowly

Liquid with high vapour

pressure used

Use positive displacement

system, eg. STARLAB

Dispenser RepeatOne®

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