MRC PCR-IF-8C Manual de usuario

Operation Manual

PCR-IF-8C

Isothermal Fluorescence PCR

PLEASE READ THIS MANUAL CAREFULLYBEFORE OPERATION

Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529 [email protected]

MRC.2.22

Foreword

First, Thank you for purchasing our Isothermal Fluorescence PCR.

Before to use the Instrument, please read this manual carefully. In particular, the

highlighted safety knowledge and requirements. This manual describes the

principle, performance, instrument and software operation of the instrument,

simple fault analysis, transportation and storage requirements, etc.

This instrument is non-IVD instrument. It is limited to

scientific research and teaching purposes and shall not

be used in medical diagnosis

Opening check

Please check the instrument and accessories with the packing list when you first

open the packing case. If you find any wrong or missing, please contact the

distributor or the manufacturer.

.Company: MRC CO., LTD

Important Guidelines

important safety operation

Please have a comprehensive understanding of how to use before the operation.

Therefore, read this manual carefully before using it.

Any improper operation before reading the manual is forbidden,

otherwise instrument may cause injured or electric shock. Please

read below safety tips and guidelines, and take precautions to

ensure safety.

Using life

See instrument label for details.

Security

The operation, maintenance and repair of the instrument should comply with the

basic guidelines and the remarked warning below. Otherwise, the instrument might

affect the protection provided by the instrument and the intended use of the

instrument.

The instrument conform to classⅠof GB 4793.1 standard. Suggest

use this instrument indoors or outdoors place where avoid light.

Disassemble the instruments without permission is forbidden. This

will make you lose the warranty qualification or have the risk of

electric shock. If repair is needed, please contact the distributor or

the manufacturer.

Make sure the voltage of the power supply is consistent with the

required voltage of the instrument before connecting the power

supply. And make sure the rated load of the power outlet is not less

than required by the instrument.

If the power cord is broken, please replace it with the same type

and specification power cord. Do not cover anything on the

instruments when using. And do not put the power cable in the

place where personnel ambulates.

Insert and pull the power line with hand gently and make sure the

plug completely insert to the jack.

III

The sample holder and heat lid are hot and may cause the scald

when the instrument is working, please make sure the

temperature is cool when you touching them.

The instrument should be kept in a place with low humidity and

dust. Away from water, direct sunlight and strong light source. The

room should be well ventilated, free of corrosive gases or strong

magnetic field interference, away from the heating furnace and

other heating sources.

The air vents on this instrument are designed for ventilation. To

avoid overheating, do not block or cover it. When many

instruments are used at the same time, the distance between each

instrument should be more than 30cm.

Placed the instrument on the horizontal and stable platform where

away from water and dust during the outdoors using. The

instrument should be kept in a cool, clean place and avoid directly

sunlight, strong light to make sure Fluorescence detection can be

used normally.

When using lithium battery, make sure the ambient temperature is

not too high, ambient humidity within allowable range, and in the

place where with less dust and away from water sources (eg:

pool, water pipe).

Power off when you finish the work. Pull off the connector plug

when there’s long time no use of the Instrument and cover it with a

soft cloth or plastic paper to prevent from dust.

Pull the connector plug from the jack at once in the following cases,

and contact the distributor or manufacturer:

There is some liquid flowing into the Instrument, drenched or fire

burned.

Abnormal operation: such as abnormal sound or smell.

Instrument dropping or outer shell damaged. The function has

obviously changed.

Clean the inspection hole and disinfect the instrument with

ultraviolet light before transport please.

Retain the original package for transport using to avoid damage

during transportation.

Warning label

Warning: Hot surface, risk of scald.

Contents

Chapter 1 Introduction............................................................................................. 1

Chapter 2 Features....................................................................................................2

Chapter 3 Accessories List........................................................................................ 4

Chapter 4 System structure and accessories........................................................... 5

Chapter 5 Operation Guide...................................................................................... 8

Chapter 6 Fault analysis and solutions...................................................................17

Chapter 7 Maintenance, storage and transportation............................................ 17

Chapter 8 Abbreviations and Tags..........................................................................21

Operation Manual Chapter 1 Introduction

Chapter 1 Introduction

PCR-IF-8C Isothermal Fluorescence PCR is contain temperature control block,

fordoptical path detection system, heating lid and analysis software. It is use

d cannisothermal amplification and fluorescence real-time detection of samples, a

s widelyimake quantitative and qualitative analysis of the amplification result. It

i,gused in rapid detection of pathogenic microorganisms(viruses, bacteria, fun

mycoplasma, parasites etc.), food hygiene inspection, environmental monitoring,

sex identification of animal embryos and detection of genetically modified food,

etc.

Working principle:

Temperature control block of the instrument heats the sample seat to heat the

tube in the seat to the needed temperature. The heating lid function can

effectively inhibit the evaporation of reagent and keep the reaction system

constant without adding paraffin oil. The optical path detection system can collect

the fluorescence intensity of the DNA containing dye or probe in the test tube at a

set time interval. The analysis software can process and transform the collected

signals, and finally output the experimental results.

Principle of optical path detection system:

1: Principle diagram of optical path detection system;

21: Plus lens; 22: Excitation filter; 23: Excitation light source

31: Collecting lens; 32: Emission filter; 33: Detector

Operation Manual Chapter 2 Features

Chapter 2 Features

2.1. Normal Operating Conditions

Ambient temperature range: 10℃~35℃, Relative humidity range: 20%~75%;

The atmospheric pressure: 860hPa~1060hPa;

Power supply: Input AC 100~240V, 50Hz~60Hz; Output DC15V, 8A;

Ambient: Dry, clean, flat and firm horizontal surface, Away from direct sunlight,

mechanical vibration and strong magnetic field interference.

2.2. Basic Parameters and Performance

Technical

parameters

Product model PCR-IF-8C

Capacity 0.2mL–8wells

(Strip of 8 tubes)

Reaction system 10-150μL

(Recommended 20-30μL)

Power supply/Power Max AC100~240V,50~60Hz/100VA

Dimensions(W*D*H) Max 145*305*100mm

Weight Max 2.5kg

Temperature

control

Heating lid function Yes, temperature is adjustable

Temperature range(℃) 15℃~99℃

Maximum heating rate(℃) ≥2℃/s

Maximum cooling rate(℃) ≥2℃/s

Temperature uniformity(℃) ±0.15℃@39℃

Temperature accuracy/

Control accuracy(℃) ±0.2℃/ 0.1℃

Optical source

Applicable dye FAM/SYBR GreenⅠ, SYTO-16, etc.

Excitation wavelength Ex470nm/LED

Detection wavelength Em535nm/PD

Function

Display 7-inch touch screen

Basic function

Program and sample parameter editing,

fluorescence acquisition, hole negative

positive and peak parameters setting,

normalization function, data management,

data exporting, amplification curve, etc.

Operation Manual Chapter 2 Features

Fusion function Analysis of melting curve and melting

peak

Result display mode 1. positive/negative; 2. peak time; 3.

Tm value

Optional software PC software

Software upgrade/Picture

updated U disk upgrade/ U disk updated

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